1502-0781

5-chloro-3-(morpholin-4-ylmethyl)-1,3-benzoxazole-2-thione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1356
Screen concentration 85.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 567702
SMILES C1COCCN1CN2C3=C(C=CC(=C3)Cl)OC2=S
Standardized SMILES Clc1ccc2OC(=S)N(CN3CCOCC3)c2c1
Molecular weight 284.7618
ALogP 3.54
H-bond donor count 0
H-bond acceptor count 4
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.49
% growth inhibition (Hom. pool) 6.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 567702
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:4.01|P-value:3.05E-5|Clearance:0.05||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC45(YLR103C)|FD-Score:3.76|P-value:8.44E-5|Clearance:0.08||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:CFT1(YDR301W)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.17||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ERG11(YHR007C)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.13||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FOL1(YNL256W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.04||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:NOC3(YLR002C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.02||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:RER2(YBR002C)|FD-Score:5.01|P-value:2.77E-7|Clearance:0.85||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPA43(YOR340C)|FD-Score:3.83|P-value:6.30E-5|Clearance:0.07||SGD DESC:RNA polymerase I subunit A43 Gene:SGV1(YPR161C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.21||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:TIF35(YDR429C)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.05||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UBC1(YDR177W)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.14||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YGR265W(YGR265W_d)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:BMS1(YPL217C)|FD-Score:4.01|P-value:3.05E-5|Clearance:0.05||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC45(YLR103C)|FD-Score:3.76|P-value:8.44E-5|Clearance:0.08||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:CFT1(YDR301W)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.17||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ERG11(YHR007C)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.13||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FOL1(YNL256W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.04||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:NOC3(YLR002C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.02||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:RER2(YBR002C)|FD-Score:5.01|P-value:2.77E-7|Clearance:0.85||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPA43(YOR340C)|FD-Score:3.83|P-value:6.30E-5|Clearance:0.07||SGD DESC:RNA polymerase I subunit A43 Gene:SGV1(YPR161C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.21||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:TIF35(YDR429C)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.05||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UBC1(YDR177W)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.14||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YGR265W(YGR265W_d)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 567702
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ATG13(YPR185W)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATX1(YNL259C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:AVO2(YMR068W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BCS1(YDR375C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:COQ10(YOL008W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COS10(YNR075W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX7(YMR256C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPR5(YDR304C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:ECM18(YDR125C)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:EEB1(YPL095C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ELF1(YKL160W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:ERV14(YGL054C)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAT1(YBR041W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FRE7(YOL152W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:FUN14(YAL008W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GCS1(YDL226C)|FD-Score:6.63|P-value:1.63E-11||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GRR1(YJR090C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX6(YDL010W)|FD-Score:-4.88|P-value:5.35E-7||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GTB1(YDR221W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:GTR2(YGR163W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HXT8(YJL214W)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IRC23(YOR044W)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:JJJ2(YJL162C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KGD2(YDR148C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:KRE2(YDR483W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LAS21(YJL062W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LYS1(YIR034C)|FD-Score:5.69|P-value:6.32E-9||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MCM21(YDR318W)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MET12(YPL023C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MFM1(YPL060W)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRPL44(YMR225C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MUD2(YKL074C)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:PCK1(YKR097W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PDR15(YDR406W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PPE1(YHR075C)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PPM1(YDR435C)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRD1(YCL057W)|FD-Score:-3.32|P-value:4.42E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PSY2(YNL201C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RCK2(YLR248W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGT1(YKL038W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RLF2(YPR018W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL31A(YDL075W)|FD-Score:-5.4|P-value:3.42E-8||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:SOL2(YCR073W-A)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STE23(YLR389C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SYO1(YDL063C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP5(YGL026C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TVP38(YKR088C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UFD2(YDL190C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMA11(YPL234C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:YBR056W(YBR056W_p)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCP4(YCR004C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR051W(YCR051W_p)|FD-Score:6.11|P-value:5.10E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR476C(YDR476C_p)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YKR078W(YKR078W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YML018C(YML018C_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR279C(YMR279C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YOR019W(YOR019W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YSP1(YHR155W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:AAT2(YLR027C)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ATG13(YPR185W)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATX1(YNL259C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:AVO2(YMR068W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BCS1(YDR375C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:COQ10(YOL008W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COS10(YNR075W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX7(YMR256C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPR5(YDR304C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:ECM18(YDR125C)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:EEB1(YPL095C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ELF1(YKL160W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:ERV14(YGL054C)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAT1(YBR041W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FRE7(YOL152W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:FUN14(YAL008W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GCS1(YDL226C)|FD-Score:6.63|P-value:1.63E-11||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GRR1(YJR090C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX6(YDL010W)|FD-Score:-4.88|P-value:5.35E-7||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GTB1(YDR221W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:GTR2(YGR163W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HXT8(YJL214W)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IRC23(YOR044W)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:JJJ2(YJL162C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KGD2(YDR148C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:KRE2(YDR483W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LAS21(YJL062W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LYS1(YIR034C)|FD-Score:5.69|P-value:6.32E-9||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MCM21(YDR318W)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MET12(YPL023C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MFM1(YPL060W)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRPL44(YMR225C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MUD2(YKL074C)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:PCK1(YKR097W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PDR15(YDR406W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PPE1(YHR075C)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PPM1(YDR435C)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRD1(YCL057W)|FD-Score:-3.32|P-value:4.42E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PSY2(YNL201C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RCK2(YLR248W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGT1(YKL038W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RLF2(YPR018W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL31A(YDL075W)|FD-Score:-5.4|P-value:3.42E-8||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:SOL2(YCR073W-A)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STE23(YLR389C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SYO1(YDL063C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP5(YGL026C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TVP38(YKR088C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UFD2(YDL190C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMA11(YPL234C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:YBR056W(YBR056W_p)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCP4(YCR004C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR051W(YCR051W_p)|FD-Score:6.11|P-value:5.10E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR476C(YDR476C_p)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YKR078W(YKR078W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YML018C(YML018C_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR279C(YMR279C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YOR019W(YOR019W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YSP1(YHR155W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C5.012.77E-70.84RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YGR265W_d4.161.58E-50.15YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YPL217C4.013.05E-50.05BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YHR007C3.963.72E-50.13ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YOR340C3.836.30E-50.07RPA43RNA polymerase I subunit A43
YLR103C3.768.44E-50.08CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
YPR161C3.691.14E-40.21SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YDR429C3.482.52E-40.05TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YLR002C3.433.02E-40.02NOC3Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
YDR301W3.413.26E-40.17CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YDR177W3.245.95E-40.14UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YNL256W3.109.65E-40.04FOL1Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
YCR093W3.060.001090.12CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YBL092W2.940.001650.14RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YJR042W2.800.002570.03NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C6.631.63E-11GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YDR435C6.232.28E-10PPM1Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
YCR051W_p6.115.10E-10YCR051W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
YIR034C5.696.32E-9LYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity
YOL152W5.181.14E-7FRE7Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels
YPR185W5.072.02E-7ATG13Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling
YDR318W4.865.94E-7MCM21Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
YLR027C4.808.12E-7AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
YKR088C4.464.01E-6TVP38Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YKL038W4.211.27E-5RGT1Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication
YDR304C4.201.34E-5CPR5Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER
YPL232W4.171.52E-5SSO1Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
YKL160W4.151.63E-5ELF1Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
YDR476C_p4.102.04E-5YDR476C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
YDR125C3.973.61E-5ECM18Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1356
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1734.08E-41SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.057971fatty acid desaturase (OLE1)
0.1552.84E-33SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.0533333fatty acid desaturase (OLE1)
0.1498.58E-31SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.130435fatty acid desaturase (OLE1)
0.1474.98E-30SGTC_258haloperidol 50.8 μMMiscellaneous35590.173913fatty acid desaturase (OLE1)
0.1372.82E-26SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.25fatty acid desaturase (OLE1)
0.1331.63E-24SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.101449plasma membrane duress
0.1284.71E-23SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.137931fatty acid desaturase (OLE1)
0.1225.39E-21SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.212121fatty acid desaturase (OLE1)
0.1211.41E-20SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0714286fatty acid desaturase (OLE1)
0.1164.76E-19SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.121212fatty acid desaturase (OLE1)
0.1158.25E-19SGTC_13081187-1617 41.4 μMChemDiv (Drug-like library)28825700.0714286fatty acid desaturase (OLE1)
0.1132.54E-18SGTC_28939048488 26.0 μMChembridge (Drug-like library)64673380.117647fatty acid desaturase (OLE1)
0.1125.43E-18SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.112676endomembrane recycling
0.1102.04E-17SGTC_2725haloperidol 53.2 μMMiscellaneous35590.173913plasma membrane duress
0.1102.70E-17SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)39653050.0615385fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21055350118200 μM0.422133313Chembridge (Fragment library)249.3322.69913
SGTC_23539059630146.67 μM0.4130434779114Chembridge (Fragment library)212.676021.59713
SGTC_14740330-01255.35 μM0.3X1474ChemDiv (Drug-like library)405.531454.46630
SGTC_13812324-006662.1 μM0.29850710885928ChemDiv (Drug-like library)402.293766.78503
SGTC_8760720-096356.8 μM0.285714765861ChemDiv (Drug-like library)319.76285.59904
SGTC_2792753523581.82 μM0.2857141281891Chembridge (Drug-like library)376.900243.43314fatty acid desaturase (OLE1)
SGTC_23479049783174.81 μM0.2807026467988Chembridge (Fragment library)267.754521.95413
SGTC_5154092-1064102 μM0.27451717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_1065prochlorperazine maleate2.48 μM0.2741946436057NIH Clinical Collection490.01482-1.33428
SGTC_1741st03813410.23 μM0.2711861272587TimTec (Natural product derivative library)393.17774.13905