1503-1303

3-(2-methoxyphenyl)-2-sulfanylidene-1,3-thiazolidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1357
Screen concentration 39.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 292895
SMILES COC1=CC=CC=C1N2C(=O)CSC2=S
Standardized SMILES COc1ccccc1N2C(=O)CSC2=S
Molecular weight 239.314
ALogP 2.71
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.1
% growth inhibition (Hom. pool) 7.51


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 292895
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:4.86|P-value:5.74E-7|Clearance:0.43||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:10.3|P-value:2.46E-25|Clearance:5.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC23(YGL128C)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.04||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:INN1(YNL152W)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.01||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:MAS1(YLR163C)|FD-Score:3.74|P-value:9.06E-5|Clearance:0.19||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MIF2(YKL089W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:MSS4(YDR208W)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.03||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:NOP56(YLR197W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSA2(YER126C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.01||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:PKC1(YBL105C)|FD-Score:5.22|P-value:8.78E-8|Clearance:0.36||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.16||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL3(YOR063W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RPP1(YHR062C)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.06||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:-3.5|P-value:2.36E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SEC24(YIL109C)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.06||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SNP1(YIL061C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TEN1(YLR010C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.19||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TIF35(YDR429C)|FD-Score:-4.7|P-value:1.30E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:4.43|P-value:4.68E-6|Clearance:0.23||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRE2(YOR256C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TRS130(YMR218C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.01||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:CCT4(YDL143W)|FD-Score:4.86|P-value:5.74E-7|Clearance:0.43||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:10.3|P-value:2.46E-25|Clearance:5.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC23(YGL128C)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.04||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:INN1(YNL152W)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.01||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:MAS1(YLR163C)|FD-Score:3.74|P-value:9.06E-5|Clearance:0.19||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MIF2(YKL089W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:MSS4(YDR208W)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.03||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:NOP56(YLR197W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSA2(YER126C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.01||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:PKC1(YBL105C)|FD-Score:5.22|P-value:8.78E-8|Clearance:0.36||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.16||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL3(YOR063W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RPP1(YHR062C)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.06||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:-3.5|P-value:2.36E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SEC24(YIL109C)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.06||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SNP1(YIL061C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TEN1(YLR010C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.19||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TIF35(YDR429C)|FD-Score:-4.7|P-value:1.30E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:4.43|P-value:4.68E-6|Clearance:0.23||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRE2(YOR256C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TRS130(YMR218C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.01||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 292895
Download HOP data (tab-delimited text)  (excel)
Gene:AIM13(YFR011C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIM36(YMR157C)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALF1(YNL148C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASI1(YMR119W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATP20(YPR020W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:BCK1(YJL095W)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CIN2(YPL241C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLB1(YGR108W)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLB5(YPR120C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COQ1(YBR003W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CST6(YIL036W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DIE2(YGR227W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ERG4(YGL012W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY1(YPR062W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FIN1(YDR130C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FKH1(YIL131C)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FMP41(YNL168C_p)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV8(YGR196C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL3(YDR009W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication Gene:GCV1(YDR019C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GDS1(YOR355W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPD2(YOL059W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:GPM3(YOL056W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GYP1(YOR070C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOS1(YPR068C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HOT13(YKL084W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HST3(YOR025W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:HTZ1(YOL012C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:HUG1(YML058W-A)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:ILM1(YJR118C)|FD-Score:5.23|P-value:8.42E-8||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:ILV1(YER086W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IOC4(YMR044W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRS4(YKR019C)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIN82(YCR091W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LDB7(YBL006C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LNP1(YHR192W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LTE1(YAL024C)|FD-Score:7.14|P-value:4.81E-13||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS1(YIR034C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAD2(YJL030W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MBA1(YBR185C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MMS1(YPR164W)|FD-Score:5.59|P-value:1.12E-8||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MMS4(YBR098W)|FD-Score:7.04|P-value:9.77E-13||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MOH1(YBL049W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MUS81(YDR386W)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPC2(YDL046W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OTU1(YFL044C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAL1(YDR348C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PEX10(YDR265W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PNG1(YPL096W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:RAD50(YNL250W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:5.81|P-value:3.22E-9||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD59(YDL059C)|FD-Score:4.34|P-value:7.26E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:REV3(YPL167C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGP1(YDR137W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT2(YDL138W)|FD-Score:-3.8|P-value:7.13E-5||SGD DESC:Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication Gene:RPA12(YJR063W)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL31A(YDL075W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.23|P-value:2.32E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT101(YJL047C)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RTT107(YHR154W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SBH1(YER087C-B)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SDP1(YIL113W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SIP18(YMR175W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SLM1(YIL105C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SNF3(YDL194W)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNO2(YNL334C)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SPF1(YEL031W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT7(YBR081C)|FD-Score:4.27|P-value:9.84E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:STP3(YLR375W)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SVL3(YPL032C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SYF2(YGR129W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAE1(YBR261C)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:6.89|P-value:2.74E-12||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI74(YDR438W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:URE2(YNL229C)|FD-Score:7.05|P-value:8.94E-13||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS3(YDR495C)|FD-Score:-4.33|P-value:7.34E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS61(YDR136C_d)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XRS2(YDR369C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR099C(YBR099C_d)|FD-Score:3.78|P-value:7.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR182C-A(YBR182C-A_p)|FD-Score:5.12|P-value:1.57E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK3(YER123W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCP4(YCR004C)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR250C(YDR250C_d)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER184C(YER184C_p)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YGL010W(YGL010W_p)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR111W(YGR111W_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR126W(YGR126W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YKL031W(YKL031W_d)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLL044W(YLL044W_d)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YML096W(YML096W_p)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR090W(YMR090W_p)|FD-Score:4.4|P-value:5.45E-6||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR279C(YMR279C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL324W(YNL324W_d)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:4.89|P-value:5.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR200W(YOR200W_d)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:7.44|P-value:5.15E-14||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL191C(YPL191C_p)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR123C(YPR123C_d)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT6(YLR262C)|FD-Score:-3.17|P-value:7.49E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YSP3(YOR003W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:AIM13(YFR011C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIM36(YMR157C)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALF1(YNL148C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASI1(YMR119W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATP20(YPR020W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:BCK1(YJL095W)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CIN2(YPL241C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLB1(YGR108W)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLB5(YPR120C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COQ1(YBR003W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CST6(YIL036W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DIE2(YGR227W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ERG4(YGL012W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY1(YPR062W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FIN1(YDR130C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FKH1(YIL131C)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FMP41(YNL168C_p)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV8(YGR196C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL3(YDR009W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication Gene:GCV1(YDR019C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GDS1(YOR355W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPD2(YOL059W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:GPM3(YOL056W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GYP1(YOR070C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOS1(YPR068C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HOT13(YKL084W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HST3(YOR025W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:HTZ1(YOL012C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:HUG1(YML058W-A)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:ILM1(YJR118C)|FD-Score:5.23|P-value:8.42E-8||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:ILV1(YER086W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IOC4(YMR044W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRS4(YKR019C)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIN82(YCR091W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LDB7(YBL006C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LNP1(YHR192W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LTE1(YAL024C)|FD-Score:7.14|P-value:4.81E-13||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS1(YIR034C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAD2(YJL030W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MBA1(YBR185C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MMS1(YPR164W)|FD-Score:5.59|P-value:1.12E-8||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MMS4(YBR098W)|FD-Score:7.04|P-value:9.77E-13||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MOH1(YBL049W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MUS81(YDR386W)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPC2(YDL046W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OTU1(YFL044C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAL1(YDR348C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PEX10(YDR265W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PNG1(YPL096W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:RAD50(YNL250W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:5.81|P-value:3.22E-9||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD59(YDL059C)|FD-Score:4.34|P-value:7.26E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:REV3(YPL167C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGP1(YDR137W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT2(YDL138W)|FD-Score:-3.8|P-value:7.13E-5||SGD DESC:Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication Gene:RPA12(YJR063W)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL31A(YDL075W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.23|P-value:2.32E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT101(YJL047C)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RTT107(YHR154W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SBH1(YER087C-B)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SDP1(YIL113W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SIP18(YMR175W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SLM1(YIL105C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SNF3(YDL194W)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNO2(YNL334C)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SPF1(YEL031W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT7(YBR081C)|FD-Score:4.27|P-value:9.84E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:STP3(YLR375W)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SVL3(YPL032C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SYF2(YGR129W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAE1(YBR261C)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:6.89|P-value:2.74E-12||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI74(YDR438W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:URE2(YNL229C)|FD-Score:7.05|P-value:8.94E-13||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS3(YDR495C)|FD-Score:-4.33|P-value:7.34E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS61(YDR136C_d)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XRS2(YDR369C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR099C(YBR099C_d)|FD-Score:3.78|P-value:7.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR182C-A(YBR182C-A_p)|FD-Score:5.12|P-value:1.57E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK3(YER123W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCP4(YCR004C)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR250C(YDR250C_d)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER184C(YER184C_p)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YGL010W(YGL010W_p)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR111W(YGR111W_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR126W(YGR126W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YKL031W(YKL031W_d)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLL044W(YLL044W_d)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YML096W(YML096W_p)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR090W(YMR090W_p)|FD-Score:4.4|P-value:5.45E-6||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR279C(YMR279C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL324W(YNL324W_d)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:4.89|P-value:5.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR200W(YOR200W_d)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:7.44|P-value:5.15E-14||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL191C(YPL191C_p)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR123C(YPR123C_d)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT6(YLR262C)|FD-Score:-3.17|P-value:7.49E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YSP3(YOR003W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative precursor to the subtilisin-like protease III

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR064W10.302.46E-255.11CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YBL105C5.228.78E-80.36PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YDL143W4.865.74E-70.43CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNL131W4.434.68E-60.23TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YHR062C4.201.35E-50.06RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR208W4.131.79E-50.03MSS4Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
YJL008C4.112.02E-50.20CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YBR192W3.914.66E-50.16RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YLR163C3.749.06E-50.19MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YIL061C3.551.90E-40.02SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YLR197W3.532.06E-40.00NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YLR010C3.532.08E-40.19TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YOR063W3.344.20E-40.03RPL3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus
YIL109C3.314.64E-40.06SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YNL152W3.255.77E-40.01INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W7.445.15E-14YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YAL024C7.144.81E-13LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YNL229C7.058.94E-13URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YBR098W7.049.77E-13MMS4Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YBR069C6.892.74E-12TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YDL020C6.232.32E-10RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR076W5.813.22E-9RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YPR164W5.591.12E-8MMS1Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
YJR118C5.238.42E-8ILM1Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
YBR182C-A_p5.121.57E-7YBR182C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YHR025W5.111.59E-7THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YDR386W5.081.86E-7MUS81Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YOL050C_d4.895.15E-7YOL050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YML096W_p4.711.25E-6YML096W_pPutative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
YIR034C4.701.31E-6LYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity

GO enrichment analysis for SGTC_1357
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2441.61E-80SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.121212
0.1583.03E-34SGTC_244nsc-207895 1.3 μMMiscellaneous426400.121212DNA damage response
0.1506.08E-31SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.111111
0.1432.13E-28SGTC_1082camptothecin 424.9 nMTimTec (Natural product library)25380.128205DNA damage response
0.1428.14E-28SGTC_2659sanguinarine 24.2 μMMicrosource (Natural product library)133941490.0769231
0.1419.45E-28SGTC_1875nocodazole 6.0 μMMiscellaneous41220.112676tubulin folding & SWR complex
0.1381.21E-26SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.119048DNA damage response
0.1361.23E-25SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.107143
0.1347.19E-25SGTC_1773st048542 73.6 μMTimTec (Natural product derivative library)32371380.15
0.1316.21E-24SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.107143
0.1301.39E-23SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.126761DNA damage response
0.1263.10E-22SGTC_22147179297 48.3 μMChembridge (Fragment library)7697440.150943superoxide
0.1248.49E-22SGTC_24305571206 200.0 μMMiscellaneous17317710.190476
0.1241.01E-21SGTC_315k915-0449 187.0 μMChemDiv (Drug-like library)209702150.173333
0.1203.41E-20SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.25

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_10234482-388816 μM0.6279073303168ChemDiv (Drug-like library)267.324062.58205TSC3-RPN4
SGTC_8630466-0191129 μM0.416667765160ChemDiv (Drug-like library)268.267381.67614cell wall
SGTC_11403229-18482.98 μM0.4130432849525ChemDiv (Drug-like library)254.285542.62105
SGTC_20645220758200 μM0.346154788518Chembridge (Fragment library)263.312221.92615
SGTC_11483909-766327.7 μM0.3207554084526ChemDiv (Drug-like library)320.38833.70205
SGTC_13021159-0335255 μM0.314815649284ChemDiv (Drug-like library)314.358963.68715
SGTC_21896047310154.43 μM0.301887711535Chembridge (Fragment library)243.300982.08903RPP1 & pyrimidine depletion
SGTC_20335192550145 μM0.297872182073Chembridge (Fragment library)189.210541.14602
SGTC_2914130-127835.5 μM0.2857141068044ChemDiv (Drug-like library)375.806223.41505Golgi
SGTC_8900929-000647.2 μM0.2857141378169ChemDiv (Drug-like library)267.324062.58325