1582-0056

7-chloro-N-(2-methylphenyl)-4-nitro-2,1,3-benzoxadiazol-5-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1359
Screen concentration 520.0 nM
Source ChemDiv (Drug-like library)
PubChem CID 3661189
SMILES CC1=CC=CC=C1NC2=C(C3=NON=C3C(=C2)Cl)[N+](=O)[O-]
Standardized SMILES Cc1ccccc1Nc2cc(Cl)c3nonc3c2[N+](=O)[O-]
Molecular weight 304.6886
ALogP 3.99
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.85
% growth inhibition (Hom. pool) 4.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3661189
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.19|P-value:7.19E-4|Clearance:0.03||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:DYS1(YHR068W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.1||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG11(YHR007C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.09||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:LST8(YNL006W)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.05||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MEX67(YPL169C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.04||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:POL5(YEL055C)|FD-Score:-3.22|P-value:6.43E-4|Clearance:0||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRE3(YJL001W)|FD-Score:-3.58|P-value:1.71E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:QRI1(YDL103C)|FD-Score:4.47|P-value:3.84E-6|Clearance:0.6||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:SCL1(YGL011C)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.08||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC26(YDR238C)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.23||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SSY5(YJL156C)|FD-Score:3.23|P-value:6.27E-4|Clearance:0.04||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TAF5(YBR198C)|FD-Score:3.15|P-value:8.07E-4|Clearance:0.05||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:UTP8(YGR128C)|FD-Score:6.45|P-value:5.57E-11|Clearance:1.75||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VTI1(YMR197C)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.1||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:3.87|P-value:5.37E-5|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJR023C(YJR023C_d)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:ACC1(YNR016C)|FD-Score:3.19|P-value:7.19E-4|Clearance:0.03||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:DYS1(YHR068W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.1||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG11(YHR007C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.09||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:LST8(YNL006W)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.05||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MEX67(YPL169C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.04||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:POL5(YEL055C)|FD-Score:-3.22|P-value:6.43E-4|Clearance:0||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRE3(YJL001W)|FD-Score:-3.58|P-value:1.71E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:QRI1(YDL103C)|FD-Score:4.47|P-value:3.84E-6|Clearance:0.6||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:SCL1(YGL011C)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.08||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC26(YDR238C)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.23||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SSY5(YJL156C)|FD-Score:3.23|P-value:6.27E-4|Clearance:0.04||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TAF5(YBR198C)|FD-Score:3.15|P-value:8.07E-4|Clearance:0.05||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:UTP8(YGR128C)|FD-Score:6.45|P-value:5.57E-11|Clearance:1.75||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VTI1(YMR197C)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.1||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:3.87|P-value:5.37E-5|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJR023C(YJR023C_d)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3661189
Download HOP data (tab-delimited text)  (excel)
Gene:AGE1(YDR524C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif Gene:AIM45(YPR004C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ALB1(YJL122W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:AMD1(YML035C)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ARP1(YHR129C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BUD26(YDR241W_d)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COG1(YGL223C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPS1(YJL172W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CWC15(YDR163W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:EMI1(YDR512C)|FD-Score:5.96|P-value:1.25E-9||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG5(YMR015C)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAU1(YER183C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FCY1(YPR062W)|FD-Score:-4.18|P-value:1.49E-5||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FYV6(YNL133C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GCV3(YAL044C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GRR1(YJR090C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX6(YDL010W)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HPT1(YDR399W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:HRK1(YOR267C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress Gene:KRE2(YDR483W)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LDB7(YBL006C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LPP1(YDR503C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:LSM7(YNL147W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAL32(YBR299W)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCH4(YOL119C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM32(YOR147W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MRPL20(YKR085C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO5(YMR109W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NDE2(YDL085W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NFI1(YOR156C)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NPC2(YDL046W)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OCA6(YDR067C_p)|FD-Score:-4.31|P-value:8.15E-6||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PDB1(YBR221C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDR11(YIL013C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PSO2(YMR137C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD33(YML011C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RBS1(YDL189W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:SDC25(YLL016W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SHE4(YOR035C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIP1(YDR422C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SOD1(YJR104C)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STP22(YCL008C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR2(YDR297W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TRP3(YKL211C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS24(YKL041W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS3(YDR495C)|FD-Score:5.71|P-value:5.58E-9||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR023C(YCR023C)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDL119C(YDL119C_p)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR455C(YDR455C_d)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER097W(YER097W_d)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR137W(YGR137W_d)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL017W(YHL017W_p)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIR024C(YIR024C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YKL091C(YKL091C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR282C(YLR282C_d)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNR005C(YNR005C_d)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR071C(YNR071C_p)|FD-Score:5.29|P-value:6.11E-8||SGD DESC:Putative aldose 1-epimerase Gene:YOR385W(YOR385W_p)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:5.65|P-value:7.90E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZRT1(YGL255W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:AGE1(YDR524C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif Gene:AIM45(YPR004C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ALB1(YJL122W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:AMD1(YML035C)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ARP1(YHR129C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BUD26(YDR241W_d)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COG1(YGL223C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPS1(YJL172W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CWC15(YDR163W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:EMI1(YDR512C)|FD-Score:5.96|P-value:1.25E-9||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG5(YMR015C)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAU1(YER183C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FCY1(YPR062W)|FD-Score:-4.18|P-value:1.49E-5||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FYV6(YNL133C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GCV3(YAL044C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GRR1(YJR090C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX6(YDL010W)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HPT1(YDR399W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:HRK1(YOR267C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress Gene:KRE2(YDR483W)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LDB7(YBL006C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LPP1(YDR503C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:LSM7(YNL147W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAL32(YBR299W)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCH4(YOL119C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM32(YOR147W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MRPL20(YKR085C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO5(YMR109W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NDE2(YDL085W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NFI1(YOR156C)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NPC2(YDL046W)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OCA6(YDR067C_p)|FD-Score:-4.31|P-value:8.15E-6||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PDB1(YBR221C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDR11(YIL013C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PSO2(YMR137C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD33(YML011C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RBS1(YDL189W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:SDC25(YLL016W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SHE4(YOR035C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIP1(YDR422C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SOD1(YJR104C)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STP22(YCL008C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR2(YDR297W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TRP3(YKL211C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS24(YKL041W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS3(YDR495C)|FD-Score:5.71|P-value:5.58E-9||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR023C(YCR023C)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDL119C(YDL119C_p)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR455C(YDR455C_d)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER097W(YER097W_d)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR137W(YGR137W_d)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL017W(YHL017W_p)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIR024C(YIR024C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YKL091C(YKL091C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR282C(YLR282C_d)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNR005C(YNR005C_d)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR071C(YNR071C_p)|FD-Score:5.29|P-value:6.11E-8||SGD DESC:Putative aldose 1-epimerase Gene:YOR385W(YOR385W_p)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:5.65|P-value:7.90E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZRT1(YGL255W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR128C6.455.57E-111.75UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR238C4.701.30E-60.23SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YDL103C4.473.84E-60.60QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YDL152W_d3.875.37E-50.28YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YHR007C3.591.67E-40.09ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YGL011C3.502.31E-40.08SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YHR068W3.423.08E-40.10DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YPL169C3.324.44E-40.04MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YNL006W3.285.19E-40.05LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YJL156C3.236.27E-40.04SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YNR016C3.197.19E-40.03ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YBR198C3.158.07E-40.05TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YMR197C3.119.50E-40.10VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YDL064W3.000.001340.05UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YMR211W2.950.001590.12DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR512C5.961.25E-9EMI1Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs
YDR495C5.715.58E-9VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YKL126W5.657.90E-9YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YNR071C_p5.296.11E-8YNR071C_pPutative aldose 1-epimerase
YBR299W5.151.33E-7MAL32Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
YML035C5.052.23E-7AMD1AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
YGL005C4.865.99E-7COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YEL045C_d4.731.11E-6YEL045C_dDubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress
YER097W_d4.631.86E-6YER097W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJR102C4.424.91E-6VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YJR104C4.298.98E-6SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YCR023C4.299.06E-6YCR023CVacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
YLL016W4.231.15E-5SDC25Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
YDL094C_d4.221.23E-5YDL094C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential
YIL013C4.151.69E-5PDR11ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth

GO enrichment analysis for SGTC_1359
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0952.64E-13SGTC_463ikarugamyin 104.0 μMICCB bioactive library54714554NA
0.0903.45E-12SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.111111
0.0831.46E-10SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.0705882
0.0822.79E-10SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0804598
0.0823.43E-10SGTC_2671danthron 100.0 μMMicrosource (Natural product library)29500.0892857DNA damage response
0.0814.10E-10SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.0860215azole & statin
0.0807.15E-10SGTC_1974st069448 26.9 μMTimTec (Natural product derivative library)63235870.025974
0.0808.26E-10SGTC_1573oleylamine 1.1 μMTimTec (Pure natural product library)53567890.0169492
0.0782.18E-9SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.121212
0.0765.23E-9SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.0437956
0.0757.18E-9SGTC_14133910-0535 34.4 μMChemDiv (Drug-like library)28769510.153846
0.0741.22E-8SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.115385calcium & mitochondrial duress
0.0731.68E-8SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0454545Golgi
0.0715.14E-8SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.1125Golgi
0.0707.76E-8SGTC_21966573537 42.7 μMChembridge (Fragment library)29030870.290909copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8201582-006059.9 μM0.53848840ChemDiv (Drug-like library)290.661983.50816
SGTC_300r073-00115.04 μM0.4444442735780ChemDiv (Drug-like library)282.683043.03806
SGTC_524r073-001027.8 μM0.4363642772106ChemDiv (Drug-like library)284.655861.80807
SGTC_720r052-164449.9 μM0.4210531422863ChemDiv (Drug-like library)289.652461.98118TSC3-RPN4
SGTC_8151582-002736.9 μM0.3548393128206ChemDiv (Drug-like library)286.24292.82717
SGTC_9671306-0166352 μM0.3448284437607ChemDiv (Drug-like library)246.265163.37524
SGTC_2840988-010432.6 μM0.3389834666532ChemDiv (Drug-like library)329.3525.28314heme requiring
SGTC_13191306-015543.7 μM0.3103453122360ChemDiv (Drug-like library)252.657063.06724
SGTC_260302-0163425.16 μM0.2982463100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_1764st04450293.5 μM0.2941188407TimTec (Natural product derivative library)214.223.27413