1611-0397

(4-methylphenyl) N-phenylcarbamate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1362
Screen concentration 101.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 27816
SMILES CC1=CC=C(C=C1)OC(=O)NC2=CC=CC=C2
Standardized SMILES Cc1ccc(OC(=O)Nc2ccccc2)cc1
Molecular weight 227.2585
ALogP 3.66
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.82
% growth inhibition (Hom. pool) 1.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 27816
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.03||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:BET1(YIL004C)|FD-Score:3.1|P-value:9.62E-4|Clearance:0.1||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CCT8(YJL008C)|FD-Score:3.81|P-value:7.02E-5|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC28(YBR160W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.12||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:CFT1(YDR301W)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.04||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:DPS1(YLL018C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:FAS2(YPL231W)|FD-Score:4.47|P-value:3.93E-6|Clearance:0.57||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:FCF1(YDR339C)|FD-Score:-3.21|P-value:6.68E-4|Clearance:0||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:HRP1(YOL123W)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:IMP4(YNL075W)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.13||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:KEI1(YDR367W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.24||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:RIA1(YNL163C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.03||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RPN11(YFR004W)|FD-Score:-3.25|P-value:5.76E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:YLR458W(YLR458W_d)|FD-Score:-3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YOR203W(YOR203W_d)|FD-Score:4.38|P-value:6.01E-6|Clearance:0.57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YTH1(YPR107C)|FD-Score:-3.76|P-value:8.41E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:APC1(YNL172W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.03||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:BET1(YIL004C)|FD-Score:3.1|P-value:9.62E-4|Clearance:0.1||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CCT8(YJL008C)|FD-Score:3.81|P-value:7.02E-5|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC28(YBR160W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.12||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:CFT1(YDR301W)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.04||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:DPS1(YLL018C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:FAS2(YPL231W)|FD-Score:4.47|P-value:3.93E-6|Clearance:0.57||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:FCF1(YDR339C)|FD-Score:-3.21|P-value:6.68E-4|Clearance:0||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:HRP1(YOL123W)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:IMP4(YNL075W)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.13||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:KEI1(YDR367W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.24||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:RIA1(YNL163C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.03||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RPN11(YFR004W)|FD-Score:-3.25|P-value:5.76E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:YLR458W(YLR458W_d)|FD-Score:-3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YOR203W(YOR203W_d)|FD-Score:4.38|P-value:6.01E-6|Clearance:0.57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YTH1(YPR107C)|FD-Score:-3.76|P-value:8.41E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 27816
Download HOP data (tab-delimited text)  (excel)
Gene:ALD5(YER073W)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:CHO2(YGR157W)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:COG1(YGL223C)|FD-Score:-4.57|P-value:2.49E-6||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FAA1(YOR317W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FMC1(YIL098C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:FRE3(YOR381W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FSF1(YOR271C_p)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GCV3(YAL044C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GIT1(YCR098C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLG2(YJL137C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HBT1(YDL223C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HMO1(YDR174W)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IRC20(YLR247C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:KCS1(YDR017C)|FD-Score:6.51|P-value:3.68E-11||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KRE1(YNL322C)|FD-Score:4.8|P-value:7.85E-7||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KXD1(YGL079W_p)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LGE1(YPL055C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MPD2(YOL088C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRF1(YGL143C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPL20(YKR085C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSW1(YDR268W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MSY1(YPL097W)|FD-Score:4.34|P-value:7.28E-6||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTF2(YDL044C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NAM2(YLR382C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OAR1(YKL055C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PAC11(YDR488C)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PAL1(YDR348C)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PDE1(YGL248W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PDR11(YIL013C)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PHO81(YGR233C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIL1(YGR086C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PPH3(YDR075W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PUG1(YER185W)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RGP1(YDR137W)|FD-Score:5.66|P-value:7.46E-9||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL19A(YBR084C-A)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:RSC2(YLR357W)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SHE4(YOR035C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SMI1(YGR229C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SOL3(YHR163W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SSK1(YLR006C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SUR2(YDR297W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TAT1(YBR069C)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA9(YML081W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TOM6(YOR045W)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRE1(YPL176C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VAC8(YEL013W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMA13(YPR036W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA4(YOR332W)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS45(YGL095C)|FD-Score:6.28|P-value:1.70E-10||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS69(YPR087W_d)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YAP1802(YGR241C)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBR062C(YBR062C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YHR097C(YHR097C_p)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL001W(YIL001W_p)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL077W-B(YJL077W-B_p)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKR075C(YKR075C_p)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YMC1(YPR058W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YMR320W(YMR320W_d)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ALD5(YER073W)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:CHO2(YGR157W)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:COG1(YGL223C)|FD-Score:-4.57|P-value:2.49E-6||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FAA1(YOR317W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FMC1(YIL098C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:FRE3(YOR381W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FSF1(YOR271C_p)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GCV3(YAL044C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GIT1(YCR098C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLG2(YJL137C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HBT1(YDL223C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HMO1(YDR174W)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IRC20(YLR247C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:KCS1(YDR017C)|FD-Score:6.51|P-value:3.68E-11||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KRE1(YNL322C)|FD-Score:4.8|P-value:7.85E-7||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KXD1(YGL079W_p)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LGE1(YPL055C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MPD2(YOL088C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRF1(YGL143C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPL20(YKR085C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSW1(YDR268W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MSY1(YPL097W)|FD-Score:4.34|P-value:7.28E-6||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTF2(YDL044C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NAM2(YLR382C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OAR1(YKL055C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PAC11(YDR488C)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PAL1(YDR348C)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PDE1(YGL248W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PDR11(YIL013C)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PHO81(YGR233C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIL1(YGR086C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PPH3(YDR075W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PUG1(YER185W)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RGP1(YDR137W)|FD-Score:5.66|P-value:7.46E-9||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL19A(YBR084C-A)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:RSC2(YLR357W)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SHE4(YOR035C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SMI1(YGR229C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SOL3(YHR163W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SSK1(YLR006C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SUR2(YDR297W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TAT1(YBR069C)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA9(YML081W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TOM6(YOR045W)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRE1(YPL176C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VAC8(YEL013W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMA13(YPR036W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA4(YOR332W)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS45(YGL095C)|FD-Score:6.28|P-value:1.70E-10||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS69(YPR087W_d)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YAP1802(YGR241C)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBR062C(YBR062C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YHR097C(YHR097C_p)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL001W(YIL001W_p)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL077W-B(YJL077W-B_p)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKR075C(YKR075C_p)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YMC1(YPR058W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YMR320W(YMR320W_d)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL231W4.473.93E-60.57FAS2Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
YOR203W_d4.386.01E-60.57YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
YJL008C3.817.02E-50.13CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNL172W3.681.16E-40.03APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YNL163C3.651.32E-40.03RIA1Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
YDR367W3.621.48E-40.23KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YLL018C3.383.57E-40.00DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YDR301W3.383.63E-40.04CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YBR160W3.344.13E-40.12CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YNL075W3.236.23E-40.13IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YIL004C3.109.62E-40.10BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YBR002C3.000.001350.11RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDR311W2.890.001910.14TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YGR024C2.750.003000.01THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
YBR154C2.740.003060.02RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR017C6.513.68E-11KCS1Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance
YGL095C6.281.70E-10VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YDR137W5.667.46E-9RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YDR075W5.191.04E-7PPH3Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
YHR097C_p4.983.21E-7YHR097C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus
YNL322C4.807.85E-7KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YBR069C4.701.29E-6TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YOR332W4.612.04E-6VMA4Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
YIL013C4.503.36E-6PDR11ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth
YDR348C4.503.39E-6PAL1Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
YIL001W_p4.376.11E-6YIL001W_pPutative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
YPL097W4.347.28E-6MSY1Mitochondrial tyrosyl-tRNA synthetase
YER073W4.318.18E-6ALD5Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
YKR075C_p4.279.62E-6YKR075C_pProtein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YEL013W4.221.24E-5VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions

GO enrichment analysis for SGTC_1362
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0903.86E-12SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0769231
0.0864.38E-11SGTC_390dec-rvkr-cmk 16.7 μMMiscellaneous99620750.0632911calcium & mitochondrial duress
0.0723.09E-8SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.125
0.0612.38E-6SGTC_29819013952 39.0 μMChembridge (Drug-like library)29963440.259259
0.0612.83E-6SGTC_8490386-0493 241.0 μMChemDiv (Drug-like library)7261190.130435
0.0588.01E-6SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.320755amide catabolism
0.0571.14E-5SGTC_1466k213-0079 57.5 μMChemDiv (Drug-like library)36579810.303571
0.0543.05E-5SGTC_7083448-5400 146.0 μMChemDiv (Drug-like library)34621950.164557
0.0526.20E-5SGTC_1517deoxycholic acid methyl ester 49.2 μMTimTec (Pure natural product library)2293460.0657895
0.0526.88E-5SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.0508475
0.0526.99E-5SGTC_28659030313 52.0 μMChembridge (Drug-like library)425627540.272727
0.0517.71E-5SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.27451
0.0518.09E-5SGTC_1901st060216 58.6 μMTimTec (Natural product derivative library)6887920.183673
0.0518.27E-5SGTC_14914138-0063 26.5 μMChemDiv (Drug-like library)238832270.046875
0.0519.01E-5SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.0967742

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8710574-0004149 μM0.4468099551173ChemDiv (Drug-like library)306.315363.83514
SGTC_3206911435049.47 μM0.42222217087243Chembridge (Drug-like library)283.321783.53513
SGTC_3260913710949.47 μM0.41860517478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_2887904478458.44 μM0.4130436465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_2882905804458.44 μM0.40425518076804Chembridge (Drug-like library)273.3021433.62513
SGTC_2794778538227.27 μM0.3921572205081Chembridge (Drug-like library)396.45953.57225
SGTC_2886905894451.95 μM0.3913044949929Chembridge (Drug-like library)299.364243.92713
SGTC_2980901434225.29 μM0.3913042996599Chembridge (Drug-like library)347.363983.94214
SGTC_3203911354349.47 μM0.38636417018012Chembridge (Drug-like library)241.28513.08412
SGTC_29087930301100 μM0.3846152223178Chembridge (Drug-like library)387.431143.00533