1672-3266

N-(6-methoxypyridazin-3-yl)-4-[(4-phenylmethoxyphenyl)methylideneamino]benzenesulfonamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1365
Screen concentration 55.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 6415873
SMILES COC1=NN=C(C=C1)NS(=O)(=O)C2=CC=C(C=C2)N=CC3=CC=C(C=C3)OCC4=CC=CC=C4
Standardized SMILES COc1ccc(NS(=O)(=O)c2ccc(cc2)N=Cc3ccc(OCc4ccccc4)cc3)nn1
Molecular weight 474.5316
ALogP 4.93
H-bond donor count 1
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.77
% growth inhibition (Hom. pool) 5.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6415873
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCL1(YPR025C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.05||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC23(YHR166C)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.06||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC45(YLR103C)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:CDC48(YDL126C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.04||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:ERV1(YGR029W)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.21||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:GNA1(YFL017C)|FD-Score:-3.39|P-value:3.50E-4|Clearance:0||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:INN1(YNL152W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.04||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:IRR1(YIL026C)|FD-Score:4.48|P-value:3.80E-6|Clearance:0.3||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:6.23|P-value:2.30E-10|Clearance:1.76||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MOT1(YPL082C)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.06||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MPP10(YJR002W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.05||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NOP58(YOR310C)|FD-Score:3.87|P-value:5.34E-5|Clearance:0.14||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:OLE1(YGL055W)|FD-Score:-6.6|P-value:2.08E-11|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RAT1(YOR048C)|FD-Score:-3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RPL25(YOL127W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RSC58(YLR033W)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:-3.19|P-value:7.20E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC26(YDR238C)|FD-Score:3.73|P-value:9.49E-5|Clearance:0.04||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SLD5(YDR489W)|FD-Score:-3.1|P-value:9.62E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SNP1(YIL061C)|FD-Score:-3.18|P-value:7.49E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TAF4(YMR005W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.04||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TAF9(YMR236W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:YDL196W(YDL196W_d)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR053W(YDR053W_d)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.12||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YJR023C(YJR023C_d)|FD-Score:3.3|P-value:4.77E-4|Clearance:0.08||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YLR076C(YLR076C_d)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YML6(YML025C)|FD-Score:-4.19|P-value:1.39E-5|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:ASA1(YPR085C)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCL1(YPR025C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.05||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC23(YHR166C)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.06||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC45(YLR103C)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:CDC48(YDL126C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.04||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:ERV1(YGR029W)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.21||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:GNA1(YFL017C)|FD-Score:-3.39|P-value:3.50E-4|Clearance:0||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:INN1(YNL152W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.04||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:IRR1(YIL026C)|FD-Score:4.48|P-value:3.80E-6|Clearance:0.3||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:6.23|P-value:2.30E-10|Clearance:1.76||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MOT1(YPL082C)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.06||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MPP10(YJR002W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.05||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NOP58(YOR310C)|FD-Score:3.87|P-value:5.34E-5|Clearance:0.14||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:OLE1(YGL055W)|FD-Score:-6.6|P-value:2.08E-11|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RAT1(YOR048C)|FD-Score:-3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RPL25(YOL127W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RSC58(YLR033W)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:-3.19|P-value:7.20E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC26(YDR238C)|FD-Score:3.73|P-value:9.49E-5|Clearance:0.04||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SLD5(YDR489W)|FD-Score:-3.1|P-value:9.62E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SNP1(YIL061C)|FD-Score:-3.18|P-value:7.49E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TAF4(YMR005W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.04||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TAF9(YMR236W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:YDL196W(YDL196W_d)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR053W(YDR053W_d)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.12||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YJR023C(YJR023C_d)|FD-Score:3.3|P-value:4.77E-4|Clearance:0.08||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YLR076C(YLR076C_d)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YML6(YML025C)|FD-Score:-4.19|P-value:1.39E-5|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6415873
Download HOP data (tab-delimited text)  (excel)
Gene:AGE2(YIL044C)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM6(YDL237W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALD6(YPL061W)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG17(YLR423C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG8(YBL078C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:ATP5(YDR298C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BLI1(YKL061W_p)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNA5(YLR231C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BRO1(YPL084W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CMR3(YPR013C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CTI6(YPL181W)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYM1(YDR430C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DOA4(YDR069C)|FD-Score:5.63|P-value:8.97E-9||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FMP52(YER004W)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FSH2(YMR222C)|FD-Score:4.95|P-value:3.80E-7||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:FUN14(YAL008W)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Mitochondrial protein of unknown function Gene:GIS2(YNL255C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLY1(YEL046C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSP2(YOR185C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAL9(YOL089C)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HUB1(YNR032C-A)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:IBA57(YJR122W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IKI3(YLR384C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:KAP104(YBR017C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:KTI11(YBL071W-A)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LSC2(YGR244C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LTV1(YKL143W)|FD-Score:4.34|P-value:7.24E-6||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MYO4(YAL029C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NFI1(YOR156C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NIP100(YPL174C)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NUR1(YDL089W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OCA6(YDR067C_p)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OPI9(YLR338W_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OYE2(YHR179W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PDB1(YBR221C)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PHO87(YCR037C)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:REI1(YBR267W)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPL40B(YKR094C)|FD-Score:4.95|P-value:3.77E-7||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS6B(YBR181C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SDP1(YIL113W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEM1(YDR363W-A)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPT10(YJL127C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SPT7(YBR081C)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SWS2(YNL081C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA3(YHR009C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TIF4631(YGR162W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBP1(YDL122W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UGA3(YDL170W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VMA4(YOR332W)|FD-Score:-4.37|P-value:6.16E-6||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YCH1(YGR203W)|FD-Score:-3.93|P-value:4.32E-5||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YEF1(YEL041W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YGR066C(YGR066C_p)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function Gene:YIL025C(YIL025C_d)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL058W(YIL058W_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL096C(YIL096C_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YIP5(YGL161C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YPK3(YBR028C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL080C(YPL080C_d)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL245W(YPL245W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR064W(YPR064W_d)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR077C(YPR077C_d)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT35(YHR105W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:AGE2(YIL044C)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM6(YDL237W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALD6(YPL061W)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG17(YLR423C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG8(YBL078C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:ATP5(YDR298C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BLI1(YKL061W_p)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNA5(YLR231C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BRO1(YPL084W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CMR3(YPR013C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CTI6(YPL181W)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYM1(YDR430C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DOA4(YDR069C)|FD-Score:5.63|P-value:8.97E-9||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FMP52(YER004W)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FSH2(YMR222C)|FD-Score:4.95|P-value:3.80E-7||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:FUN14(YAL008W)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Mitochondrial protein of unknown function Gene:GIS2(YNL255C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLY1(YEL046C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSP2(YOR185C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAL9(YOL089C)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HUB1(YNR032C-A)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:IBA57(YJR122W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IKI3(YLR384C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:KAP104(YBR017C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:KTI11(YBL071W-A)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LSC2(YGR244C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LTV1(YKL143W)|FD-Score:4.34|P-value:7.24E-6||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MYO4(YAL029C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NFI1(YOR156C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NIP100(YPL174C)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NUR1(YDL089W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OCA6(YDR067C_p)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OPI9(YLR338W_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OYE2(YHR179W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PDB1(YBR221C)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PHO87(YCR037C)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:REI1(YBR267W)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPL40B(YKR094C)|FD-Score:4.95|P-value:3.77E-7||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS6B(YBR181C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SDP1(YIL113W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEM1(YDR363W-A)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPT10(YJL127C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SPT7(YBR081C)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SWS2(YNL081C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA3(YHR009C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TIF4631(YGR162W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBP1(YDL122W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UGA3(YDL170W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VMA4(YOR332W)|FD-Score:-4.37|P-value:6.16E-6||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YCH1(YGR203W)|FD-Score:-3.93|P-value:4.32E-5||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YEF1(YEL041W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YGR066C(YGR066C_p)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function Gene:YIL025C(YIL025C_d)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL058W(YIL058W_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL096C(YIL096C_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YIP5(YGL161C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YPK3(YBR028C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL080C(YPL080C_d)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL245W(YPL245W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR064W(YPR064W_d)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR077C(YPR077C_d)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT35(YHR105W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR367W6.232.30E-101.76KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YIL026C4.483.80E-60.30IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDL126C4.181.48E-50.04CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YGR029W4.141.74E-50.21ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YHR166C3.934.22E-50.06CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YOR310C3.875.34E-50.14NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YDR238C3.739.49E-50.04SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YJR002W3.691.12E-40.05MPP10Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
YPR025C3.641.35E-40.05CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
YNL152W3.591.65E-40.04INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YMR236W3.551.90E-40.02TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YLR076C_d3.532.06E-40.11YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YDR053W_d3.423.11E-40.12YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
YJR023C_d3.304.77E-40.08YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YOL127W3.236.28E-40.08RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR037C7.331.18E-13PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YDR069C5.638.97E-9DOA4Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
YKR094C4.953.77E-7RPL40BUbiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
YMR222C4.953.80E-7FSH2Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
YBR081C4.552.68E-6SPT7Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
YBR221C4.552.71E-6PDB1E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria
YLR425W4.464.05E-6TUS1Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
YJR122W4.444.51E-6IBA57Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
YPL174C4.424.92E-6NIP100Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
YKL143W4.347.24E-6LTV1Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature
YGR162W4.241.10E-5TIF4631Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
YKL061W_p4.241.13E-5BLI1_pPutative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
YGL161C4.231.16E-5YIP5Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
YNL255C4.211.27E-5GIS2Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
YBR181C4.072.36E-5RPS6BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1365
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1194.74E-20SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0859375
0.0672.14E-7SGTC_1832st054113 55.0 μMTimTec (Natural product derivative library)12512670.0947368
0.0663.79E-7SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.0859375
0.0613.08E-6SGTC_21165511452 16.9 μMChembridge (Fragment library)8707440.0675676
0.0571.34E-5SGTC_13331482-0047 286.0 μMChemDiv (Drug-like library)38111160.0243902
0.0552.50E-5SGTC_1950st077106 7.2 μMTimTec (Natural product derivative library)138318640.0666667RNA processing & uracil transport
0.0543.26E-5SGTC_12280124-0032 52.5 μMChemDiv (Drug-like library)X12280.0704225
0.0543.42E-5SGTC_21045389443 200.0 μMChembridge (Fragment library)22445360.075
0.0518.99E-5SGTC_12540550-0035 264.0 μMChemDiv (Drug-like library)151214350.0759494
0.0501.07E-4SGTC_21645685496 200.0 μMChembridge (Fragment library)7590460.0823529
0.0501.41E-4SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.082352960S ribosome export
0.0491.66E-4SGTC_1683970-0862 530.5 μMChemDiv (Drug-like library)31351860.06
0.0482.13E-4SGTC_1867st057372 64.0 μMTimTec (Natural product derivative library)6888370.126582
0.0473.03E-4SGTC_1946st076057 65.1 μMTimTec (Natural product derivative library)9530090.0666667
0.0473.50E-4SGTC_13441488-0097 29.1 μMChemDiv (Drug-like library)7479660.0843373

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3061672-343611.6 μM0.5211276797378ChemDiv (Drug-like library)542.201162.93628Golgi
SGTC_7700389-112167.2 μM0.46753108ChemDiv (Drug-like library)464.49373.42529RPP1 & pyrimidine depletion
SGTC_15101123-020071.5 μM0.3934435378812ChemDiv (Drug-like library)313.392365.39602
SGTC_1631benzylvanillin81.9 μM0.31343375506TimTec (Natural product derivative library)242.269863.1403Golgi
SGTC_3272913741349.47 μM0.30882417614870Chembridge (Drug-like library)321.391362.6415
SGTC_6491123-0311367 μM0.304348693629ChemDiv (Drug-like library)241.28513.51613endomembrane recycling
SGTC_1994063-002441.6 μM0.294118306215ChemDiv (Drug-like library)311.783863.31414
SGTC_860417-1630471.92 μM0.2820513634867ChemDiv (Drug-like library)401.58367.402ubiquinone biosynthesis & proteasome
SGTC_9501165-050894.1 μM0.281696148369ChemDiv (Drug-like library)303.376082.59214
SGTC_1708st03323431.83 μM0.277778674664TimTec (Natural product derivative library)321.165923.88803