1852-0141

2,7-diamino-4-(2-fluorophenyl)-4H-chromene-3-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1368
Screen concentration 284.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2838987
SMILES C1=CC=C(C(=C1)C2C3=C(C=C(C=C3)N)OC(=C2C#N)N)F
Standardized SMILES NC1=C(C#N)C(c2ccccc2F)c3ccc(N)cc3O1
Molecular weight 281.2844
ALogP 2.32
H-bond donor count 2
H-bond acceptor count 5
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.78
% growth inhibition (Hom. pool) 2.5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2838987
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:-3.21|P-value:6.53E-4|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CDC15(YAR019C)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.03||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.01||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CRM1(YGR218W)|FD-Score:3.82|P-value:6.70E-5|Clearance:0.1||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:GWT1(YJL091C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.25||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MRD1(YPR112C)|FD-Score:4.87|P-value:5.44E-7|Clearance:0.83||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:ROK1(YGL171W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.08||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:SEC2(YNL272C)|FD-Score:3.46|P-value:2.65E-4|Clearance:0.02||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:TAF9(YMR236W)|FD-Score:-3.24|P-value:5.98E-4|Clearance:0||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TRL1(YJL087C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:YGR114C(YGR114C_d)|FD-Score:-3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR190C(YGR190C_d)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YNL181W(YNL181W_p)|FD-Score:3.37|P-value:3.76E-4|Clearance:0.08||SGD DESC:Putative oxidoreductase; required for cell viability Gene:YOR102W(YOR102W_d)|FD-Score:4.04|P-value:2.62E-5|Clearance:0.2||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ACC1(YNR016C)|FD-Score:-3.21|P-value:6.53E-4|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CDC15(YAR019C)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.03||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.01||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CRM1(YGR218W)|FD-Score:3.82|P-value:6.70E-5|Clearance:0.1||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:GWT1(YJL091C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.25||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MRD1(YPR112C)|FD-Score:4.87|P-value:5.44E-7|Clearance:0.83||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:ROK1(YGL171W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.08||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:SEC2(YNL272C)|FD-Score:3.46|P-value:2.65E-4|Clearance:0.02||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:TAF9(YMR236W)|FD-Score:-3.24|P-value:5.98E-4|Clearance:0||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TRL1(YJL087C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:YGR114C(YGR114C_d)|FD-Score:-3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR190C(YGR190C_d)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YNL181W(YNL181W_p)|FD-Score:3.37|P-value:3.76E-4|Clearance:0.08||SGD DESC:Putative oxidoreductase; required for cell viability Gene:YOR102W(YOR102W_d)|FD-Score:4.04|P-value:2.62E-5|Clearance:0.2||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2838987
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM37(YNL100W)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ASM4(YDL088C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATP22(YDR350C)|FD-Score:-4.35|P-value:6.80E-6||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BMH1(YER177W)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC26(YFR036W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHA1(YCL064C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CIR2(YOR356W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:COX13(YGL191W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:COX8(YLR395C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DDR2(YOL052C-A)|FD-Score:-4.53|P-value:2.92E-6||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DNL4(YOR005C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:DUG2(YBR281C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF6(YJR082C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:EFG1(YGR271C-A)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERG2(YMR202W)|FD-Score:14.2|P-value:2.67E-46||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.51|P-value:1.84E-8||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GEA1(YJR031C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GSP2(YOR185C)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HFM1(YGL251C)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HMI1(YOL095C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HRD1(YOL013C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HST2(YPL015C)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HTL1(YCR020W-B)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IRC18(YJL037W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISR1(YPR106W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KEL3(YPL263C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LAP3(YNL239W)|FD-Score:5.5|P-value:1.85E-8||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LCB5(YLR260W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEM3(YNL323W)|FD-Score:5.66|P-value:7.78E-9||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIF1(YGL090W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:MCK1(YNL307C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MCM21(YDR318W)|FD-Score:-5.54|P-value:1.52E-8||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MEF1(YLR069C)|FD-Score:6.11|P-value:4.97E-10||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:NIP100(YPL174C)|FD-Score:6.65|P-value:1.50E-11||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NKP2(YLR315W)|FD-Score:7.92|P-value:1.18E-15||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NOP13(YNL175C)|FD-Score:5.41|P-value:3.13E-8||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NUM1(YDR150W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PAU11(YGL261C_p)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDR11(YIL013C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PFK1(YGR240C)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PKR1(YMR123W)|FD-Score:6|P-value:9.90E-10||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POL4(YCR014C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PTR2(YKR093W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PYK2(YOR347C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:QCR6(YFR033C)|FD-Score:6.6|P-value:2.04E-11||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RCY1(YJL204C)|FD-Score:-3.9|P-value:4.84E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHB1(YCR027C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL13B(YMR142C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:5|P-value:2.81E-7||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7B(YNL096C)|FD-Score:-3.74|P-value:9.38E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTC2(YBR147W)|FD-Score:6.09|P-value:5.82E-10||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTN2(YDL204W)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SCT1(YBL011W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SLM6(YBR266C_d)|FD-Score:-4.82|P-value:7.07E-7||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SOK1(YDR006C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPO1(YNL012W)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPR3(YGR059W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SUR4(YLR372W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SVL3(YPL032C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TAH1(YCR060W)|FD-Score:-4.53|P-value:2.93E-6||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:TDA11(YHR159W_p)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TED1(YIL039W)|FD-Score:-6.65|P-value:1.49E-11||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRM8(YDL201W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBX2(YML013W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UBX4(YMR067C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VMA1(YDL185W)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:6.08|P-value:6.15E-10||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:5.81|P-value:3.17E-9||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS30(YPL120W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS45(YGL095C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAR035C-A(YAR035C-A_p)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR056W(YBR056W_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCK3(YER123W)|FD-Score:3.84|P-value:6.14E-5||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR001W(YCR001W_d)|FD-Score:4.4|P-value:5.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:7.64|P-value:1.05E-14||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL180W(YDL180W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR179W-A(YDR179W-A_p)|FD-Score:-4.95|P-value:3.79E-7||SGD DESC:Putative protein of unknown function Gene:YFL019C(YFL019C_d)|FD-Score:-3.98|P-value:3.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFL041W-A(YFL041W-A_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR130C(YGR130C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHL041W(YHL041W_d)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:5.8|P-value:3.38E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL222C(YKL222C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKR018C(YKR018C_p)|FD-Score:6.51|P-value:3.78E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR278C(YLR278C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YMR087W(YMR087W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR105W-A(YMR105W-A_p)|FD-Score:6.2|P-value:2.79E-10||SGD DESC:Putative protein of unknown function Gene:YOL098C(YOL098C_p)|FD-Score:-4.84|P-value:6.37E-7||SGD DESC:Putative metalloprotease Gene:YOR139C(YOR139C_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL247C(YPL247C_p)|FD-Score:6.02|P-value:8.47E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR076W(YPR076W_d)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YRB30(YGL164C)|FD-Score:4.83|P-value:6.91E-7||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:ACB1(YGR037C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM37(YNL100W)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ASM4(YDL088C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATP22(YDR350C)|FD-Score:-4.35|P-value:6.80E-6||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BMH1(YER177W)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC26(YFR036W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHA1(YCL064C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CIR2(YOR356W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:COX13(YGL191W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:COX8(YLR395C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DDR2(YOL052C-A)|FD-Score:-4.53|P-value:2.92E-6||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DNL4(YOR005C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:DUG2(YBR281C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF6(YJR082C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:EFG1(YGR271C-A)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERG2(YMR202W)|FD-Score:14.2|P-value:2.67E-46||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.51|P-value:1.84E-8||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GEA1(YJR031C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GSP2(YOR185C)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HFM1(YGL251C)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HMI1(YOL095C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HRD1(YOL013C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HST2(YPL015C)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HTL1(YCR020W-B)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IRC18(YJL037W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISR1(YPR106W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KEL3(YPL263C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LAP3(YNL239W)|FD-Score:5.5|P-value:1.85E-8||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LCB5(YLR260W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEM3(YNL323W)|FD-Score:5.66|P-value:7.78E-9||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIF1(YGL090W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:MCK1(YNL307C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MCM21(YDR318W)|FD-Score:-5.54|P-value:1.52E-8||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MEF1(YLR069C)|FD-Score:6.11|P-value:4.97E-10||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:NIP100(YPL174C)|FD-Score:6.65|P-value:1.50E-11||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NKP2(YLR315W)|FD-Score:7.92|P-value:1.18E-15||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NOP13(YNL175C)|FD-Score:5.41|P-value:3.13E-8||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NUM1(YDR150W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PAU11(YGL261C_p)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDR11(YIL013C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PFK1(YGR240C)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PKR1(YMR123W)|FD-Score:6|P-value:9.90E-10||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POL4(YCR014C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PTR2(YKR093W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PYK2(YOR347C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:QCR6(YFR033C)|FD-Score:6.6|P-value:2.04E-11||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RCY1(YJL204C)|FD-Score:-3.9|P-value:4.84E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHB1(YCR027C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL13B(YMR142C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:5|P-value:2.81E-7||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7B(YNL096C)|FD-Score:-3.74|P-value:9.38E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTC2(YBR147W)|FD-Score:6.09|P-value:5.82E-10||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTN2(YDL204W)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SCT1(YBL011W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SLM6(YBR266C_d)|FD-Score:-4.82|P-value:7.07E-7||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SOK1(YDR006C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPO1(YNL012W)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPR3(YGR059W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SUR4(YLR372W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SVL3(YPL032C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TAH1(YCR060W)|FD-Score:-4.53|P-value:2.93E-6||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:TDA11(YHR159W_p)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TED1(YIL039W)|FD-Score:-6.65|P-value:1.49E-11||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRM8(YDL201W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBX2(YML013W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UBX4(YMR067C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VMA1(YDL185W)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:6.08|P-value:6.15E-10||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:5.81|P-value:3.17E-9||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS30(YPL120W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS45(YGL095C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAR035C-A(YAR035C-A_p)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR056W(YBR056W_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCK3(YER123W)|FD-Score:3.84|P-value:6.14E-5||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR001W(YCR001W_d)|FD-Score:4.4|P-value:5.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:7.64|P-value:1.05E-14||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL180W(YDL180W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR179W-A(YDR179W-A_p)|FD-Score:-4.95|P-value:3.79E-7||SGD DESC:Putative protein of unknown function Gene:YFL019C(YFL019C_d)|FD-Score:-3.98|P-value:3.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFL041W-A(YFL041W-A_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR130C(YGR130C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHL041W(YHL041W_d)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:5.8|P-value:3.38E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL222C(YKL222C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKR018C(YKR018C_p)|FD-Score:6.51|P-value:3.78E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR278C(YLR278C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YMR087W(YMR087W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR105W-A(YMR105W-A_p)|FD-Score:6.2|P-value:2.79E-10||SGD DESC:Putative protein of unknown function Gene:YOL098C(YOL098C_p)|FD-Score:-4.84|P-value:6.37E-7||SGD DESC:Putative metalloprotease Gene:YOR139C(YOR139C_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL247C(YPL247C_p)|FD-Score:6.02|P-value:8.47E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR076W(YPR076W_d)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YRB30(YGL164C)|FD-Score:4.83|P-value:6.91E-7||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR112C4.875.44E-70.83MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YOR102W_d4.042.62E-50.20YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YAR019C3.855.97E-50.03CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YGR218W3.826.70E-50.10CRM1Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
YJL091C3.721.01E-40.25GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YNL272C3.462.65E-40.02SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YGL171W3.452.81E-40.08ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YNL181W_p3.373.76E-40.08YNL181W_pPutative oxidoreductase; required for cell viability
YGR190C_d3.295.00E-40.19YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YOR257W3.109.69E-40.01CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YDL209C3.090.001010.15CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YIL026C2.940.001660.12IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YPL238C_d2.820.002420.02YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YBL014C2.800.002580.03RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YMR028W2.770.002830.02TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W14.202.67E-46ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YLR315W7.921.18E-15NKP2Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
YCR023C7.641.05E-14YCR023CVacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
YPL174C6.651.50E-11NIP100Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
YFR033C6.602.04E-11QCR6Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
YKR018C_p6.513.78E-11YKR018C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
YMR105W-A_p6.202.79E-10YMR105W-A_pPutative protein of unknown function
YLR069C6.114.97E-10MEF1Mitochondrial elongation factor involved in translational elongation
YBR147W6.095.82E-10RTC2Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter
YPR036W6.086.15E-10VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YPL247C_p6.028.47E-10YPL247C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
YMR123W6.009.90E-10PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YGR020C5.813.17E-9VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YJL120W_d5.803.38E-9YJL120W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YNL323W5.667.78E-9LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane

GO enrichment analysis for SGTC_1368
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2004.23E-54SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0545455ERG2
0.1972.09E-52SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0757576ERG2
0.1895.22E-48SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.0759494ERG2
0.1874.62E-47SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0657895ERG2
0.1841.03E-45SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0921053ERG2
0.1812.56E-44SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.0864198ERG2
0.1771.04E-42SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0943396
0.1763.33E-42SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.326923
0.1743.98E-41SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0909091ERG2
0.1725.58E-40SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.075ERG2
0.1625.85E-36SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.015625ERG2
0.1555.10E-33SGTC_9741326-0144 138.0 μMChemDiv (Drug-like library)54118560.0634921ERG2
0.1521.18E-31SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.0547945ERG2
0.1477.77E-30SGTC_2732terfenadine 20.0 μMMiscellaneous54050.0769231ERG2
0.1441.64E-28SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.0540541ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13691866-0067208 μM0.6938783531355ChemDiv (Drug-like library)315.7294432.98525RPP1 & pyrimidine depletion
SGTC_9221866-0035480 μM0.5686273156169ChemDiv (Drug-like library)305.373663.30924excess fatty acid
SGTC_2074521200775.4 μM0.5758611Chembridge (Fragment library)272.3007631.66826
SGTC_2691st07735660.2 μM0.4655172839272TimTec (Natural product derivative library)332.276653.56227
SGTC_1978st07176142 μM0.4385965147680TimTec (Natural product derivative library)292.331843.56224TSC3-RPN4
SGTC_1664st0142179.02 μM0.3846153982782TimTec (Natural product derivative library)413.1967034.0151660S ribosome export
SGTC_2067522462934.08 μM0.3728812835000Chembridge (Fragment library)288.728922.15414
SGTC_1668st01491451.6 μM0.3650792839556TimTec (Natural product derivative library)366.368863.50415RPP1 & pyrimidine depletion
SGTC_2035515446394.6 μM0.3508772769623Chembridge (Fragment library)254.283861.48914
SGTC_1687st02077855.36 μM0.3283584259299TimTec (Natural product derivative library)336.38443.93225