2910-0861

(3-fluorophenyl)-[4-[(2-methoxynaphthalen-1-yl)methyl]piperazin-1-yl]methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1376
Screen concentration 41.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 1112859
SMILES COC1=C(C2=CC=CC=C2C=C1)CN3CCN(CC3)C(=O)C4=CC(=CC=C4)F
Standardized SMILES COc1ccc2ccccc2c1CN3CCN(CC3)C(=O)c4cccc(F)c4
Molecular weight 378.4393
ALogP 3.88
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.75
% growth inhibition (Hom. pool) 8.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1112859
Download HIP data (tab-delimited text)  (excel)
Gene:KRE5(YOR336W)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NUP116(YMR047C)|FD-Score:3.3|P-value:4.82E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) Gene:PDC2(YDR081C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.3||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:POP6(YGR030C)|FD-Score:3.84|P-value:6.07E-5|Clearance:0.3||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRT1(YOR361C)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.3||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:PTA1(YAL043C)|FD-Score:-3.49|P-value:2.46E-4|Clearance:0||SGD DESC:Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII Gene:SPT16(YGL207W)|FD-Score:3.95|P-value:3.96E-5|Clearance:0.3||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TAM41(YGR046W)|FD-Score:3.85|P-value:5.89E-5|Clearance:0.3||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TFC3(YAL001C)|FD-Score:3.96|P-value:3.71E-5|Clearance:0.3||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UBC1(YDR177W)|FD-Score:3.77|P-value:8.23E-5|Clearance:0.3||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UFE1(YOR075W)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.15||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:KRE5(YOR336W)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NUP116(YMR047C)|FD-Score:3.3|P-value:4.82E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) Gene:PDC2(YDR081C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.3||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:POP6(YGR030C)|FD-Score:3.84|P-value:6.07E-5|Clearance:0.3||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRT1(YOR361C)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.3||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:PTA1(YAL043C)|FD-Score:-3.49|P-value:2.46E-4|Clearance:0||SGD DESC:Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII Gene:SPT16(YGL207W)|FD-Score:3.95|P-value:3.96E-5|Clearance:0.3||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TAM41(YGR046W)|FD-Score:3.85|P-value:5.89E-5|Clearance:0.3||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TFC3(YAL001C)|FD-Score:3.96|P-value:3.71E-5|Clearance:0.3||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UBC1(YDR177W)|FD-Score:3.77|P-value:8.23E-5|Clearance:0.3||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UFE1(YOR075W)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.15||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1112859
Download HOP data (tab-delimited text)  (excel)
Gene:ACK1(YDL203C)|FD-Score:-4.33|P-value:7.58E-6||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ADA2(YDR448W)|FD-Score:4.76|P-value:9.85E-7||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE12(YNL220W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AGP3(YFL055W)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:BNA7(YDR428C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BUG1(YDL099W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CAD1(YDR423C)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CCW12(YLR110C)|FD-Score:4.95|P-value:3.66E-7||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHL1(YPL008W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:COG8(YML071C)|FD-Score:6.91|P-value:2.37E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSR2(YPR030W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CYC7(YEL039C)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAS1(YJL149W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DAT1(YML113W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DFM1(YDR411C)|FD-Score:4.32|P-value:7.76E-6||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DIC1(YLR348C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:EDC3(YEL015W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ENV10(YLR065C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FIG4(YNL325C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GIP1(YBR045C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GIS1(YDR096W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:GOR1(YNL274C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:HIT1(YJR055W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HRD3(YLR207W)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:HSE1(YHL002W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IAH1(YOR126C)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IMA5(YJL216C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IRC21(YMR073C)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KEX1(YGL203C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KRE2(YDR483W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:MAE1(YKL029C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM1(YML104C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MGR3(YMR115W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MHF1(YOL086W-A)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MHP1(YJL042W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MIS1(YBR084W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MSS2(YDL107W)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NAB6(YML117W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NGR1(YBR212W)|FD-Score:-4.41|P-value:5.15E-6||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NRK1(YNL129W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis Gene:OPI10(YOL032W)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:ORM2(YLR350W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.82|P-value:6.80E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PDR15(YDR406W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PEX12(YMR026C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHM6(YDR281C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:POX1(YGL205W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PPA2(YMR267W)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PRS1(YKL181W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSD2(YGR170W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:QRI7(YDL104C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RGD2(YFL047W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL2B(YIL018W)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL8B(YLL045C)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:-3.97|P-value:3.54E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTN2(YDL204W)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAW1(YAL027W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SEC22(YLR268W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFA1(YDL168W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOK2(YMR016C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SPE3(YPR069C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:TED1(YIL039W)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TPO5(YKL174C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:TPP1(YMR156C)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRM8(YDL201W)|FD-Score:-4.89|P-value:5.00E-7||SGD DESC:Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP2(YER090W)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TVP18(YMR071C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VPS74(YDR372C)|FD-Score:7.36|P-value:9.32E-14||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:WAR1(YML076C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YDL027C(YDL027C_p)|FD-Score:7.98|P-value:7.40E-16||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL073W(YDL073W_p)|FD-Score:8.98|P-value:1.38E-19||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL177C(YDL177C_p)|FD-Score:4|P-value:3.14E-5||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YEL023C(YEL023C_p)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-5.43|P-value:2.84E-8||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFR018C(YFR018C_p)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Putative protein of unknown function Gene:YGL088W(YGL088W_d)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR127W(YGR127W_p)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YHL041W(YHL041W_d)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YHL042W(YHL042W_p)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL092W(YIL092W_p)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YKL100C(YKL100C_p)|FD-Score:-4.46|P-value:4.15E-6||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKL131W(YKL131W_d)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR078W(YKR078W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR169W(YLR169W_d)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR254C(YMR254C_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOL131W(YOL131W_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative protein of unknown function Gene:YOR225W(YOR225W_d)|FD-Score:4.45|P-value:4.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACK1(YDL203C)|FD-Score:-4.33|P-value:7.58E-6||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ADA2(YDR448W)|FD-Score:4.76|P-value:9.85E-7||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE12(YNL220W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AGP3(YFL055W)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:BNA7(YDR428C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BUG1(YDL099W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CAD1(YDR423C)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CCW12(YLR110C)|FD-Score:4.95|P-value:3.66E-7||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHL1(YPL008W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:COG8(YML071C)|FD-Score:6.91|P-value:2.37E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSR2(YPR030W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CYC7(YEL039C)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAS1(YJL149W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DAT1(YML113W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DFM1(YDR411C)|FD-Score:4.32|P-value:7.76E-6||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DIC1(YLR348C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:EDC3(YEL015W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ENV10(YLR065C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FIG4(YNL325C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GIP1(YBR045C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GIS1(YDR096W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:GOR1(YNL274C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:HIT1(YJR055W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HRD3(YLR207W)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:HSE1(YHL002W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IAH1(YOR126C)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IMA5(YJL216C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IRC21(YMR073C)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KEX1(YGL203C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KRE2(YDR483W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:MAE1(YKL029C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM1(YML104C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MGR3(YMR115W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MHF1(YOL086W-A)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MHP1(YJL042W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MIS1(YBR084W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MSS2(YDL107W)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NAB6(YML117W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NGR1(YBR212W)|FD-Score:-4.41|P-value:5.15E-6||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NRK1(YNL129W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis Gene:OPI10(YOL032W)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:ORM2(YLR350W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.82|P-value:6.80E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PDR15(YDR406W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PEX12(YMR026C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHM6(YDR281C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:POX1(YGL205W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PPA2(YMR267W)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PRS1(YKL181W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSD2(YGR170W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:QRI7(YDL104C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RGD2(YFL047W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL2B(YIL018W)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL8B(YLL045C)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:-3.97|P-value:3.54E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTN2(YDL204W)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAW1(YAL027W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SEC22(YLR268W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFA1(YDL168W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOK2(YMR016C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SPE3(YPR069C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:TED1(YIL039W)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TPO5(YKL174C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:TPP1(YMR156C)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRM8(YDL201W)|FD-Score:-4.89|P-value:5.00E-7||SGD DESC:Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP2(YER090W)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TVP18(YMR071C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VPS74(YDR372C)|FD-Score:7.36|P-value:9.32E-14||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:WAR1(YML076C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YDL027C(YDL027C_p)|FD-Score:7.98|P-value:7.40E-16||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL073W(YDL073W_p)|FD-Score:8.98|P-value:1.38E-19||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL177C(YDL177C_p)|FD-Score:4|P-value:3.14E-5||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YEL023C(YEL023C_p)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-5.43|P-value:2.84E-8||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFR018C(YFR018C_p)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Putative protein of unknown function Gene:YGL088W(YGL088W_d)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR127W(YGR127W_p)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YHL041W(YHL041W_d)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YHL042W(YHL042W_p)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL092W(YIL092W_p)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YKL100C(YKL100C_p)|FD-Score:-4.46|P-value:4.15E-6||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKL131W(YKL131W_d)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR078W(YKR078W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR169W(YLR169W_d)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR254C(YMR254C_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOL131W(YOL131W_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative protein of unknown function Gene:YOR225W(YOR225W_d)|FD-Score:4.45|P-value:4.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YAL001C3.963.71E-50.29TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YGL207W3.953.96E-50.29SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YGR046W3.855.89E-50.29TAM41Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
YGR030C3.846.07E-50.29POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR177W3.778.23E-50.29UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YOR361C3.661.25E-40.29PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YDR081C3.601.61E-40.29PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YMR047C3.304.82E-40.17NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YOR075W3.138.71E-40.15UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YOR250C2.980.001440.08CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDL092W2.900.001880.02SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YDL087C2.880.001980.02LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YNL262W2.870.002080.03POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YKR083C2.840.002270.01DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YMR211W2.820.002370.05DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL073W_p8.981.38E-19YDL073W_pPutative protein of unknown function; YDL073W is not an essential gene
YDL027C_p7.987.40E-16YDL027C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
YDR372C7.369.32E-14VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YML071C6.912.37E-12COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YEL039C5.561.37E-8CYC7Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication
YFL055W5.022.57E-7AGP3Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition
YLR110C4.953.66E-7CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YDR448W4.769.85E-7ADA2Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
YDL168W4.621.94E-6SFA1Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
YMR026C4.473.89E-6PEX12C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
YOR225W_d4.454.39E-6YOR225W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR126C4.434.71E-6IAH1Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
YDR411C4.327.76E-6DFM1Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
YLR268W4.211.28E-5SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YJL216C4.151.65E-5IMA5Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose

GO enrichment analysis for SGTC_1376
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0903.49E-12SGTC_3660181-0206 4.3 μMChemDiv (Drug-like library)2748740.0793651Golgi
0.0832.00E-10SGTC_29789011784 43.0 μMChembridge (Drug-like library)29949340.175676
0.0813.84E-10SGTC_15120259-0544 12.2 μMChemDiv (Drug-like library)184760.12987
0.0806.34E-10SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.115385ERAD & cell cycle
0.0781.87E-9SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.047619
0.0772.52E-9SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0652174Golgi
0.0765.78E-9SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0519481Golgi
0.0691.67E-7SGTC_9963970-0799 1.8 μMChemDiv (Drug-like library)66156480.102564
0.0663.81E-7SGTC_30539095903 49.5 μMChembridge (Drug-like library)171740180.15Golgi
0.0654.77E-7SGTC_1463448-8650 53.1 μMChemDiv (Drug-like library)28914880.133333NEO1-PIK1
0.0621.67E-6SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.117647NEO1
0.0621.77E-6SGTC_7551995-0008 37.3 μMChemDiv (Drug-like library)6895330.0821918
0.0622.10E-6SGTC_12830922-0125 16.4 μMChemDiv (Drug-like library)33608840.181818
0.0612.48E-6SGTC_31889110789 49.5 μMChembridge (Drug-like library)8492170.189189iron homeostasis
0.0596.00E-6SGTC_790052-0007 94.1 μMChemDiv (Drug-like library)28014720.123288

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7294092-058594.1 μM0.5322582169061ChemDiv (Drug-like library)390.4500234.5504
SGTC_5423381-024489.3 μM0.476191112843ChemDiv (Drug-like library)360.448863.67603
SGTC_11584092-049491.8 μM0.468751148199ChemDiv (Drug-like library)416.512124.2704
SGTC_3140909424349.47 μM0.46153819291112Chembridge (Drug-like library)342.4072233.32204
SGTC_3054909439749.47 μM0.4576272598354Chembridge (Drug-like library)330.422883.69302fatty acid desaturase (OLE1)
SGTC_13752910-0797186 μM0.428571742508ChemDiv (Drug-like library)340.416162.75204
SGTC_3158910000749.47 μM0.4062519297233Chembridge (Drug-like library)373.286844.01902
SGTC_3049909222349.47 μM0.48623917Chembridge (Drug-like library)328.835843.93502
SGTC_11594092-053861.2 μM0.365079717301ChemDiv (Drug-like library)332.7997233.65403
SGTC_3040909189449.47 μM0.34782625236966Chembridge (Drug-like library)328.471663.71503DNA intercalators