2093-0007

3,4-dichloro-1-[2-[2-[2-(3,4-dichloro-2,5-dioxopyrrol-1-yl)ethoxy]ethoxy]ethyl]pyrrole-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1378
Screen concentration 380.0 nM
Source ChemDiv (Drug-like library)
PubChem CID 3618014
SMILES C(COCCOCCN1C(=O)C(=C(C1=O)Cl)Cl)N2C(=O)C(=C(C2=O)Cl)Cl
Standardized SMILES ClC1=C(Cl)C(=O)N(CCOCCOCCN2C(=O)C(=C(Cl)C2=O)Cl)C1=O
Molecular weight 446.0669
ALogP 0.66
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.78
% growth inhibition (Hom. pool) 1.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3618014
Download HIP data (tab-delimited text)  (excel)
Gene:CDC5(YMR001C)|FD-Score:-4.41|P-value:5.22E-6|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CET1(YPL228W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.19||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:DED1(YOR204W)|FD-Score:7.1|P-value:6.36E-13|Clearance:2.53||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DHR2(YKL078W)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.07||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FAS1(YKL182W)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:JAC1(YGL018C)|FD-Score:3.32|P-value:4.50E-4|Clearance:0.09||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:KEG1(YFR042W)|FD-Score:3.97|P-value:3.52E-5|Clearance:0.11||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MCM10(YIL150C)|FD-Score:-4|P-value:3.13E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MVD1(YNR043W)|FD-Score:-3.24|P-value:5.95E-4|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PGA3(YML125C)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.01||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:PRE7(YBL041W)|FD-Score:4.56|P-value:2.51E-6|Clearance:0.45||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP3(YDR473C)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.06||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN11(YFR004W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.05||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPT3(YDR394W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.1||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:SPT16(YGL207W)|FD-Score:-3.92|P-value:4.51E-5|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TIM17(YJL143W)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.02||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TRR1(YDR353W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.16||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UBC9(YDL064W)|FD-Score:3.81|P-value:7.00E-5|Clearance:0.1||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YDR526C(YDR526C_d)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR265W(YGR265W_d)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:CDC5(YMR001C)|FD-Score:-4.41|P-value:5.22E-6|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CET1(YPL228W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.19||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:DED1(YOR204W)|FD-Score:7.1|P-value:6.36E-13|Clearance:2.53||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DHR2(YKL078W)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.07||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FAS1(YKL182W)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:JAC1(YGL018C)|FD-Score:3.32|P-value:4.50E-4|Clearance:0.09||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:KEG1(YFR042W)|FD-Score:3.97|P-value:3.52E-5|Clearance:0.11||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MCM10(YIL150C)|FD-Score:-4|P-value:3.13E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MVD1(YNR043W)|FD-Score:-3.24|P-value:5.95E-4|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PGA3(YML125C)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.01||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:PRE7(YBL041W)|FD-Score:4.56|P-value:2.51E-6|Clearance:0.45||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP3(YDR473C)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.06||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN11(YFR004W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.05||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPT3(YDR394W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.1||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:SPT16(YGL207W)|FD-Score:-3.92|P-value:4.51E-5|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TIM17(YJL143W)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.02||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TRR1(YDR353W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.16||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UBC9(YDL064W)|FD-Score:3.81|P-value:7.00E-5|Clearance:0.1||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YDR526C(YDR526C_d)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR265W(YGR265W_d)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3618014
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ABZ1(YNR033W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CRG1(YHR209W)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSN9(YDR179C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CST6(YIL036W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CYT2(YKL087C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DBF20(YPR111W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DCC1(YCL016C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DIT2(YDR402C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:ECM7(YLR443W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:GCN20(YFR009W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GIS2(YNL255C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GRX2(YDR513W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HHT2(YNL031C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HIR1(YBL008W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HST2(YPL015C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXT8(YJL214W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IDP1(YDL066W)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IDP2(YLR174W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:LCB4(YOR171C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MNN4(YKL201C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MRPL25(YGR076C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MSB3(YNL293W)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSH3(YCR092C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NUM1(YDR150W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OM45(YIL136W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:PEX18(YHR160C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX21(YGR239C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PEX29(YDR479C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PMD1(YER132C)|FD-Score:4.33|P-value:7.33E-6||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PTC5(YOR090C)|FD-Score:4.31|P-value:8.35E-6||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:REE1(YJL217W)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:REI1(YBR267W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFX1(YLR176C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM1(YCR028C-A)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIP1(YEL024W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RIT1(YMR283C)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL20A(YMR242C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SIC1(YLR079W)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SLX9(YGR081C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNQ2(YDR011W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SRP40(YKR092C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:STB4(YMR019W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STE20(YHL007C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUR4(YLR372W)|FD-Score:-4.1|P-value:2.02E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT2(YOL020W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TED1(YIL039W)|FD-Score:-6.56|P-value:2.71E-11||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TMA16(YOR252W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRM1(YDR120C)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UAF30(YOR295W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UGA1(YGR019W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress Gene:VPS36(YLR417W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS55(YJR044C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS74(YDR372C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:WSC4(YHL028W)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Putative protein of unknown function Gene:YBR134W(YBR134W_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR300C(YBR300C_d)|FD-Score:5.67|P-value:6.99E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDR282C(YDR282C_p)|FD-Score:-4.79|P-value:8.53E-7||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YFR057W(YFR057W_p)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Putative protein of unknown function Gene:YGL088W(YGL088W_d)|FD-Score:-3.99|P-value:3.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR226C(YGR226C_d)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YIL032C(YIL032C_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL007C(YJL007C_d)|FD-Score:-5.72|P-value:5.42E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKE2(YLR200W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR040C(YLR040C_p)|FD-Score:5.88|P-value:2.02E-9||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR297W(YLR297W_p)|FD-Score:5.21|P-value:9.26E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YLR311C(YLR311C_d)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL013W-B(YOL013W-B_d)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOR139C(YOR139C_d)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR223W(YOR223W)|FD-Score:4.37|P-value:6.23E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPL225W(YPL225W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR027C(YPR027C_p)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Putative protein of unknown function Gene:YSP2(YDR326C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ABM1(YJR108W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ABZ1(YNR033W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CRG1(YHR209W)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSN9(YDR179C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CST6(YIL036W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CYT2(YKL087C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DBF20(YPR111W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DCC1(YCL016C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DIT2(YDR402C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:ECM7(YLR443W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:GCN20(YFR009W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GIS2(YNL255C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GRX2(YDR513W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HHT2(YNL031C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HIR1(YBL008W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HST2(YPL015C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXT8(YJL214W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IDP1(YDL066W)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IDP2(YLR174W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:LCB4(YOR171C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MNN4(YKL201C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MRPL25(YGR076C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MSB3(YNL293W)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSH3(YCR092C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NUM1(YDR150W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OM45(YIL136W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:PEX18(YHR160C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX21(YGR239C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PEX29(YDR479C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PMD1(YER132C)|FD-Score:4.33|P-value:7.33E-6||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PTC5(YOR090C)|FD-Score:4.31|P-value:8.35E-6||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:REE1(YJL217W)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:REI1(YBR267W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFX1(YLR176C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM1(YCR028C-A)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIP1(YEL024W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RIT1(YMR283C)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL20A(YMR242C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SIC1(YLR079W)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SLX9(YGR081C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNQ2(YDR011W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SRP40(YKR092C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:STB4(YMR019W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STE20(YHL007C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUR4(YLR372W)|FD-Score:-4.1|P-value:2.02E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT2(YOL020W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TED1(YIL039W)|FD-Score:-6.56|P-value:2.71E-11||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TMA16(YOR252W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRM1(YDR120C)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UAF30(YOR295W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UGA1(YGR019W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress Gene:VPS36(YLR417W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS55(YJR044C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS74(YDR372C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:WSC4(YHL028W)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Putative protein of unknown function Gene:YBR134W(YBR134W_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR300C(YBR300C_d)|FD-Score:5.67|P-value:6.99E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDR282C(YDR282C_p)|FD-Score:-4.79|P-value:8.53E-7||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YFR057W(YFR057W_p)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Putative protein of unknown function Gene:YGL088W(YGL088W_d)|FD-Score:-3.99|P-value:3.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR226C(YGR226C_d)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YIL032C(YIL032C_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL007C(YJL007C_d)|FD-Score:-5.72|P-value:5.42E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKE2(YLR200W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR040C(YLR040C_p)|FD-Score:5.88|P-value:2.02E-9||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR297W(YLR297W_p)|FD-Score:5.21|P-value:9.26E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YLR311C(YLR311C_d)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL013W-B(YOL013W-B_d)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOR139C(YOR139C_d)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR223W(YOR223W)|FD-Score:4.37|P-value:6.23E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPL225W(YPL225W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR027C(YPR027C_p)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Putative protein of unknown function Gene:YSP2(YDR326C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR204W7.106.36E-132.53DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YBL041W4.562.51E-60.45PRE7Beta 6 subunit of the 20S proteasome
YDR473C4.111.96E-50.06PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YKL078W4.052.58E-50.07DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YFR042W3.973.52E-50.11KEG1Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
YDR526C_d3.875.53E-50.06YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL064W3.817.00E-50.10UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YDR394W3.711.05E-40.10RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YFR004W3.611.54E-40.05RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YKL182W3.561.87E-40.03FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YJL143W3.532.10E-40.02TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YPL228W3.512.27E-40.19CET1Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YGL018C3.324.50E-40.09JAC1Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
YML125C3.236.19E-40.01PGA3Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
YDR353W3.226.41E-40.17TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR040C_p5.882.02E-9YLR040C_pProtein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
YBR300C_d5.676.99E-9YBR300C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene
YLR311C_d5.591.13E-8YLR311C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR372C5.571.24E-8VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YLR297W_p5.219.26E-8YLR297W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
YFR036W5.081.89E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YNL293W4.769.70E-7MSB3Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBR134W_d4.641.74E-6YBR134W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJR084W4.493.52E-6YJR084WProtein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
YHL028W4.483.82E-6WSC4ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
YGR239C4.415.11E-6PEX21Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress
YOR223W4.376.23E-6YOR223WSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
YER132C4.337.33E-6PMD1Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions
YOR090C4.318.35E-6PTC5Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p
YMR283C4.289.23E-6RIT12'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA

GO enrichment analysis for SGTC_1378
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0751.26E-8SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)67908400.0192308redox potentiating
0.0714.63E-8SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.0571429
0.0631.29E-6SGTC_13752910-0797 186.0 μMChemDiv (Drug-like library)7425080.0892857
0.0631.35E-6SGTC_12550566-0045 221.0 μMChemDiv (Drug-like library)27884690.0980392excess fatty acid
0.0604.33E-6SGTC_30379091026 49.5 μMChembridge (Drug-like library)252363980.0819672NEO1-PIK1
0.0587.80E-6SGTC_23117694451 200.0 μMChembridge (Fragment library)21631420.122449
0.0571.23E-5SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0784314
0.0561.44E-5SGTC_8590439-0102 54.6 μMChemDiv (Drug-like library)6101430.0625redox potentiating
0.0518.31E-5SGTC_32069114350 49.5 μMChembridge (Drug-like library)170872430.0754717
0.0518.84E-5SGTC_21245246320 200.0 μMChembridge (Fragment library)5793420.0416667heme biosynthesis & mitochondrial translocase
0.0482.04E-4SGTC_11463579-1023 7.0 μMChemDiv (Drug-like library)X11460.0677966
0.0482.38E-4SGTC_20795212404 121.0 μMChembridge (Fragment library)7584620.0454545
0.0473.39E-4SGTC_8790831-1030 162.0 μMChemDiv (Drug-like library)15492520.0697674
0.0473.48E-4SGTC_5643555-0129 330.0 μMChemDiv (Drug-like library)5632600.0727273
0.0464.11E-4SGTC_1692fluoroquinolonic acid 71.0 μMTimTec (Natural product derivative library)4831800.109091

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1817st05211664.4 μM0.2448984361398TimTec (Natural product derivative library)310.73291.85514RNA processing & uracil transport
SGTC_7864544-0069423 μM0.232558748821ChemDiv (Drug-like library)268.310442.73602
SGTC_12760871-006894.2 μM0.2307693414906ChemDiv (Drug-like library)261.319562.57104
SGTC_23369001819198.65 μM0.2195122989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_2945906768161.76 μM0.21818216456930Chembridge (Drug-like library)344.792182.40113
SGTC_12830922-012516.4 μM0.2156863360884ChemDiv (Drug-like library)425.951024.84904
SGTC_2451585622345.7 μM0.2115382179896Miscellaneous269.339942.31105
SGTC_606k015-000838.6 μM0.2093021970475ChemDiv (Drug-like library)306.143463.78802
SGTC_1814st05211031.54 μM0.208333674163TimTec (Natural product derivative library)345.57641.94313RNA processing & uracil transport
SGTC_1000086-012849.59 μM0.19565293297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress