3002-2117

6-(4-methylsulfanylphenyl)imidazo[2,1-b][1,3]thiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1382
Screen concentration 298.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1497314
SMILES CSC1=CC=C(C=C1)C2=CN3C=CSC3=N2
Standardized SMILES CSc1ccc(cc1)c2cn3ccsc3n2
Molecular weight 246.3512
ALogP 2.79
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.55
% growth inhibition (Hom. pool) 4.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1497314
Download HIP data (tab-delimited text)  (excel)
Gene:CLP1(YOR250C)|FD-Score:-4.43|P-value:4.71E-6|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DPS1(YLL018C)|FD-Score:-3.28|P-value:5.15E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ERG7(YHR072W)|FD-Score:4.31|P-value:8.08E-6|Clearance:0.61||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:IMP4(YNL075W)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.88||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:PRE8(YML092C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.03||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RBA50(YDR527W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.21||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RDS3(YPR094W)|FD-Score:3.29|P-value:4.92E-4|Clearance:0.06||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RER2(YBR002C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.11||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSA4(YCR072C)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.02||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SLU7(YDR088C)|FD-Score:5.71|P-value:5.55E-9|Clearance:0.88||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SMT3(YDR510W)|FD-Score:3.5|P-value:2.37E-4|Clearance:0.17||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SNU23(YDL098C)|FD-Score:3.12|P-value:9.19E-4|Clearance:0.08||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:YDL196W(YDL196W_d)|FD-Score:-4.09|P-value:2.14E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:CLP1(YOR250C)|FD-Score:-4.43|P-value:4.71E-6|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DPS1(YLL018C)|FD-Score:-3.28|P-value:5.15E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ERG7(YHR072W)|FD-Score:4.31|P-value:8.08E-6|Clearance:0.61||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:IMP4(YNL075W)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.88||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:PRE8(YML092C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.03||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RBA50(YDR527W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.21||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RDS3(YPR094W)|FD-Score:3.29|P-value:4.92E-4|Clearance:0.06||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RER2(YBR002C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.11||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSA4(YCR072C)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.02||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SLU7(YDR088C)|FD-Score:5.71|P-value:5.55E-9|Clearance:0.88||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SMT3(YDR510W)|FD-Score:3.5|P-value:2.37E-4|Clearance:0.17||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SNU23(YDL098C)|FD-Score:3.12|P-value:9.19E-4|Clearance:0.08||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:YDL196W(YDL196W_d)|FD-Score:-4.09|P-value:2.14E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1497314
Download HOP data (tab-delimited text)  (excel)
Gene:ALE1(YOR175C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ARF1(YDL192W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ASI2(YNL159C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATG3(YNR007C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BRO1(YPL084W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BTS1(YPL069C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAT5(YOR125C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CTR1(YPR124W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DJP1(YIR004W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DOA1(YKL213C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOT6(YER088C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DSS4(YPR017C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:HAM1(YJR069C)|FD-Score:5.24|P-value:8.16E-8||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:IML3(YBR107C)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IRC14(YOR135C_d)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC7(YFR055W_p)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:LSM6(YDR378C)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MBP1(YDL056W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MEI4(YER044C-A)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MRPL38(YKL170W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:PAU8(YAL068C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions Gene:PHO85(YPL031C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLP1(YDR183W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:SHM1(YBR263W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SPO16(YHR153C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SRP40(YKR092C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSH1(YBR283C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:STE50(YCL032W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STP2(YHR006W)|FD-Score:4.83|P-value:6.92E-7||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:TGL5(YOR081C)|FD-Score:4.88|P-value:5.32E-7||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TRP1(YDR007W)|FD-Score:6.24|P-value:2.14E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TVP18(YMR071C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBP12(YJL197W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:UBP3(YER151C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS5(YOR069W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YIR020C(YIR020C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR124C(YJR124C_p)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YMR166C(YMR166C_p)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR210W(YMR210W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YOL106W(YOL106W_d)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR200W(YOR200W_d)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:ALE1(YOR175C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ARF1(YDL192W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ASI2(YNL159C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATG3(YNR007C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BRO1(YPL084W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BTS1(YPL069C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAT5(YOR125C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CTR1(YPR124W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DJP1(YIR004W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DOA1(YKL213C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOT6(YER088C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DSS4(YPR017C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:HAM1(YJR069C)|FD-Score:5.24|P-value:8.16E-8||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:IML3(YBR107C)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IRC14(YOR135C_d)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC7(YFR055W_p)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:LSM6(YDR378C)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MBP1(YDL056W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MEI4(YER044C-A)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MRPL38(YKL170W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:PAU8(YAL068C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions Gene:PHO85(YPL031C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLP1(YDR183W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:SHM1(YBR263W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SPO16(YHR153C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SRP40(YKR092C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSH1(YBR283C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:STE50(YCL032W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STP2(YHR006W)|FD-Score:4.83|P-value:6.92E-7||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:TGL5(YOR081C)|FD-Score:4.88|P-value:5.32E-7||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TRP1(YDR007W)|FD-Score:6.24|P-value:2.14E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TVP18(YMR071C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBP12(YJL197W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:UBP3(YER151C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS5(YOR069W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YIR020C(YIR020C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR124C(YJR124C_p)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YMR166C(YMR166C_p)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR210W(YMR210W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YOL106W(YOL106W_d)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR200W(YOR200W_d)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR088C5.715.55E-90.88SLU7RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
YNL075W5.201.02E-70.88IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YHR072W4.318.08E-60.61ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YDR527W3.701.07E-40.21RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YDR510W3.502.37E-40.17SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YML092C3.334.41E-40.03PRE8Alpha 2 subunit of the 20S proteasome
YPR094W3.294.92E-40.06RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YCR072C3.246.06E-40.02RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YBR002C3.226.39E-40.11RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDL098C3.129.19E-40.08SNU23Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
YJR141W_p3.030.001210.15YJR141W_pEssential protein of unknown function
YNL232W2.880.001970.11CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YKL095W2.780.002740.07YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YER012W2.700.003430.05PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YJL069C2.650.004020.06UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W6.242.14E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YJR069C5.248.16E-8HAM1Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress
YBR107C5.121.54E-7IML3Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
YOR081C4.885.32E-7TGL5Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate
YHR006W4.836.92E-7STP2Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
YEL063C4.337.34E-6CAN1Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
YHR153C4.181.44E-5SPO16Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
YFR055W_p4.141.71E-5IRC7_pBeta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner
YJL197W4.131.85E-5UBP12Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm
YPL031C4.062.48E-5PHO85Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle
YNL159C3.934.20E-5ASI2Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
YOR135C_d3.865.64E-5IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YOR125C3.855.95E-5CAT5Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
YOR069W3.816.96E-5VPS5Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
YJR124C_p3.749.37E-5YJR124C_pPutative protein of unknown function; expression induced under calcium shortage

GO enrichment analysis for SGTC_1382
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1111.43E-17SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.101695
0.0985.01E-14SGTC_18825615643 20.0 μMMiscellaneous22530750.0506329TRP & mitochondrial translation
0.0881.22E-11SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.0441176
0.0872.06E-11SGTC_20044023728 41.9 μMChembridge (Fragment library)3600840.037037
0.0856.04E-11SGTC_1069diphenylcyclopropenone 8.5 μMNIH Clinical Collection650570.0909091
0.0831.40E-10SGTC_24885268135 45.7 μMMiscellaneous12703560.0483871
0.0831.53E-10SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0519481
0.0831.74E-10SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0645161
0.0831.94E-10SGTC_13722752-0194 270.0 μMChemDiv (Drug-like library)8233340.0684932
0.0832.20E-10SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0357143TRP & mitochondrial translation
0.0806.45E-10SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.0606061TRP & mitochondrial translation
0.0807.91E-10SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0454545
0.0782.03E-9SGTC_14234048-0112 5.6 μMChemDiv (Drug-like library)X14230.0487805
0.0782.37E-9SGTC_24545-benzylidenerhodanine 10.4 μMMiscellaneous12732100.0517241TRP & mitochondrial translation
0.0731.87E-8SGTC_410478-0632 82.6 μMChemDiv (Drug-like library)37504240.0491803

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_9332914-0560114 μM0.657895463887ChemDiv (Drug-like library)234.704682.91302
SGTC_13451488-019231.4 μM0.4375658348ChemDiv (Drug-like library)280.796263.30603
SGTC_7021488-019094 μM0.314815949357ChemDiv (Drug-like library)339.273844.1103amide catabolism
SGTC_13521496-099199 μM0.31253000039ChemDiv (Drug-like library)215.274261.98603tubulin folding & SWR complex
SGTC_11941488-0194225 μM0.2456141391452ChemDiv (Drug-like library)355.276542.93205
SGTC_8800833-048787.2 μM0.23404376075ChemDiv (Drug-like library)252.334183.54513mitochondrial processes
SGTC_12240823-0416294 μM0.2264153598164ChemDiv (Drug-like library)249.312082.68606
SGTC_2176580977433.97 μM0.222870539Chembridge (Fragment library)240.752323.38123mitochondrial processes
SGTC_13441488-009729.1 μM0.214286747966ChemDiv (Drug-like library)274.768543.57502
SGTC_14484255-0083152 μM0.2093025399660ChemDiv (Drug-like library)263.290622.13723ERG2