3346-4487

N'-(2,5-dimethylphenyl)sulfonylbenzenecarboximidamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1388
Screen concentration 254.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3345614
SMILES CC1=CC(=C(C=C1)C)S(=O)(=O)N=C(C2=CC=CC=C2)N
Standardized SMILES Cc1ccc(C)c(c1)S(=O)(=O)N=C(N)c2ccccc2
Molecular weight 288.3647
ALogP 2.57
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.43
% growth inhibition (Hom. pool) 2.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3345614
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.29||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:APC5(YOR249C)|FD-Score:-3.86|P-value:5.59E-5|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:BET3(YKR068C)|FD-Score:-3.79|P-value:7.57E-5|Clearance:0||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CLP1(YOR250C)|FD-Score:-3.9|P-value:4.85E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:JIP5(YPR169W)|FD-Score:3.34|P-value:4.12E-4|Clearance:0.14||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MAK21(YDR060W)|FD-Score:-3.28|P-value:5.20E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:RLP7(YNL002C)|FD-Score:4.17|P-value:1.53E-5|Clearance:0.4||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:SEC13(YLR208W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0.4||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SGV1(YPR161C)|FD-Score:3.36|P-value:3.83E-4|Clearance:0.02||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SMC5(YOL034W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.09||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SNU114(YKL173W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SRP14(YDL092W)|FD-Score:-3.13|P-value:8.62E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TAM41(YGR046W)|FD-Score:-3.77|P-value:8.19E-5|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:ALG14(YBR070C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.29||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:APC5(YOR249C)|FD-Score:-3.86|P-value:5.59E-5|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:BET3(YKR068C)|FD-Score:-3.79|P-value:7.57E-5|Clearance:0||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CLP1(YOR250C)|FD-Score:-3.9|P-value:4.85E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:JIP5(YPR169W)|FD-Score:3.34|P-value:4.12E-4|Clearance:0.14||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MAK21(YDR060W)|FD-Score:-3.28|P-value:5.20E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:RLP7(YNL002C)|FD-Score:4.17|P-value:1.53E-5|Clearance:0.4||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:SEC13(YLR208W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0.4||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SGV1(YPR161C)|FD-Score:3.36|P-value:3.83E-4|Clearance:0.02||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SMC5(YOL034W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.09||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SNU114(YKL173W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SRP14(YDL092W)|FD-Score:-3.13|P-value:8.62E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TAM41(YGR046W)|FD-Score:-3.77|P-value:8.19E-5|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3345614
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADH6(YMR318C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADK2(YER170W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM4(YBR194W)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ALD5(YER073W)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:APM3(YBR288C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:BCH1(YMR237W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BEM4(YPL161C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BTS1(YPL069C)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBR1(YIL043C)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCM1(YGR150C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHS5(YLR330W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CLU1(YMR012W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COQ9(YLR201C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:CWC21(YDR482C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DSF2(YBR007C_p)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMP24(YGL200C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ENT2(YLR206W)|FD-Score:-3.14|P-value:8.50E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ETR1(YBR026C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:EXG2(YDR261C)|FD-Score:4.72|P-value:1.15E-6||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FOX2(YKR009C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GAT4(YIR013C)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GTT3(YEL017W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HOS2(YGL194C)|FD-Score:6.23|P-value:2.41E-10||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HTL1(YCR020W-B)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:KIN4(YOR233W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LDB18(YLL049W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:MCM21(YDR318W)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MEF1(YLR069C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHP1(YJL042W)|FD-Score:5|P-value:2.85E-7||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MMP1(YLL061W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MMS22(YLR320W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN11(YJL183W)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MOH1(YBL049W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSD1(YPL104W)|FD-Score:4.32|P-value:7.67E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MTC2(YKL098W)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTQ2(YDR140W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:PKR1(YMR123W)|FD-Score:6.16|P-value:3.75E-10||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PXA1(YPL147W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RPS0A(YGR214W)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS8A(YBL072C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SEA4(YBL104C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SER2(YGR208W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SGO1(YOR073W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHC1(YER096W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIM1(YIL123W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SNF2(YOR290C)|FD-Score:5.44|P-value:2.68E-8||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:THO2(YNL139C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRM9(YML014W)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TSR2(YLR435W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUL1(YKL034W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:VAM6(YDL077C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VBA5(YKR105C_p)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VMA22(YHR060W)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS63(YLR261C_d)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL180W(YDL180W_p)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR203W(YDR203W_d)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL068C(YJL068C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL150W(YJL150W_d)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL053C(YLL053C_p)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR312C(YLR312C_p)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YML009W-B(YML009W-B_d)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL146C-A(YNL146C-A_p)|FD-Score:5.34|P-value:4.68E-8||SGD DESC:Putative protein of unknown function Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL162C(YPL162C_p)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPR003C(YPR003C_p)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:ACE2(YLR131C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADH6(YMR318C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADK2(YER170W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM4(YBR194W)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ALD5(YER073W)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:APM3(YBR288C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:BCH1(YMR237W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BEM4(YPL161C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BTS1(YPL069C)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBR1(YIL043C)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCM1(YGR150C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHS5(YLR330W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CLU1(YMR012W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COQ9(YLR201C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:CWC21(YDR482C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DSF2(YBR007C_p)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMP24(YGL200C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ENT2(YLR206W)|FD-Score:-3.14|P-value:8.50E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ETR1(YBR026C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:EXG2(YDR261C)|FD-Score:4.72|P-value:1.15E-6||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FOX2(YKR009C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GAT4(YIR013C)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GTT3(YEL017W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HOS2(YGL194C)|FD-Score:6.23|P-value:2.41E-10||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HTL1(YCR020W-B)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:KIN4(YOR233W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LDB18(YLL049W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:MCM21(YDR318W)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MEF1(YLR069C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHP1(YJL042W)|FD-Score:5|P-value:2.85E-7||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MMP1(YLL061W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MMS22(YLR320W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN11(YJL183W)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MOH1(YBL049W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSD1(YPL104W)|FD-Score:4.32|P-value:7.67E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MTC2(YKL098W)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTQ2(YDR140W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:PKR1(YMR123W)|FD-Score:6.16|P-value:3.75E-10||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PXA1(YPL147W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RPS0A(YGR214W)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS8A(YBL072C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SEA4(YBL104C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SER2(YGR208W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SGO1(YOR073W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHC1(YER096W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIM1(YIL123W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SNF2(YOR290C)|FD-Score:5.44|P-value:2.68E-8||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:THO2(YNL139C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRM9(YML014W)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TSR2(YLR435W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUL1(YKL034W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:VAM6(YDL077C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VBA5(YKR105C_p)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VMA22(YHR060W)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS63(YLR261C_d)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL180W(YDL180W_p)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR203W(YDR203W_d)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL068C(YJL068C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL150W(YJL150W_d)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL053C(YLL053C_p)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR312C(YLR312C_p)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YML009W-B(YML009W-B_d)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL146C-A(YNL146C-A_p)|FD-Score:5.34|P-value:4.68E-8||SGD DESC:Putative protein of unknown function Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL162C(YPL162C_p)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPR003C(YPR003C_p)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL002C4.171.53E-50.40RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YLR208W3.855.86E-50.40SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YOL034W3.452.78E-40.09SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YPR161C3.363.83E-40.02SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YPR169W3.344.12E-40.14JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YBR070C3.206.85E-40.29ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YOL066C2.910.001810.01RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YOR254C2.900.001870.04SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YJL174W2.860.002150.04KRE9Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
YHR101C2.810.002440.10BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YLR100W2.720.003310.02ERG273-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
YBR136W2.700.003470.08MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YPL146C2.620.004460.12NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YLR103C2.500.006270.01CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
YDR413C_d2.480.006520.01YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL194C6.232.41E-10HOS2Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
YMR123W6.163.75E-10PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YML014W5.611.02E-8TRM9tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
YOR290C5.442.68E-8SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
YNL146C-A_p5.344.68E-8YNL146C-A_pPutative protein of unknown function
YJL042W5.002.85E-7MHP1Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
YDR261C4.721.15E-6EXG2Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YBR026C4.691.36E-6ETR12-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
YIL123W4.691.39E-6SIM1Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
YGR208W4.542.82E-6SER2Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
YLR320W4.454.33E-6MMS22Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
YJL183W4.415.07E-6MNN11Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
YBR007C_p4.385.86E-6DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YML009W-B_d4.356.90E-6YML009W-B_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition
YPL104W4.327.67E-6MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene

GO enrichment analysis for SGTC_1388
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0863.30E-11SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.06
0.0856.49E-11SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.186441
0.0765.83E-9SGTC_7103448-4187 58.0 μMChemDiv (Drug-like library)39464120.0540541fatty acid desaturase (OLE1)
0.0756.49E-9SGTC_11841269-1718 139.0 μMChemDiv (Drug-like library)54038210.125
0.0707.75E-8SGTC_1629st005085 44.9 μMTimTec (Natural product derivative library)242070910.117647RPP1 & pyrimidine depletion
0.0708.27E-8SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.101266ERG2
0.0699.40E-8SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.116667
0.0672.42E-7SGTC_13732887-0003 58.5 μMChemDiv (Drug-like library)7769480.0694444Golgi
0.0672.58E-7SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.172414
0.0664.63E-7SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.0769231
0.0655.10E-7SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0724638
0.0647.28E-7SGTC_890302-0250 142.1 μMChemDiv (Drug-like library)42035180.0862069
0.0612.27E-6SGTC_28519015922 71.4 μMChembridge (Drug-like library)29968930.15625
0.0612.82E-6SGTC_251melphalan 456.5 μMMiscellaneous4606120.0983607DNA damage response
0.0612.96E-6SGTC_8380109-0203 71.4 μMChemDiv (Drug-like library)7594220.235294

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12640783-0040158 μM0.4883722855798ChemDiv (Drug-like library)339.207642.34613
SGTC_1691st01993384.6 μM0.3541676176082TimTec (Natural product derivative library)236.308344.67401
SGTC_770828-028612.52 μM0.296296745345ChemDiv (Drug-like library)264.275383.23803TSC3-RPN4
SGTC_5651418-000144.5 μM0.2884622862145ChemDiv (Drug-like library)245.704183.63812
SGTC_12890976-007369.1 μM0.2857143391136ChemDiv (Drug-like library)253.29582.7812
SGTC_12530550-0034176 μM0.2833335969915ChemDiv (Drug-like library)296.36363.33124
SGTC_21826325192200 μM0.27451736248Chembridge (Fragment library)226.273762.35422Golgi
SGTC_7560508-199928.3 μM0.2741946753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_20134024628200 μM0.2692315230760Chembridge (Fragment library)226.273762.35422
SGTC_3260913710949.47 μM0.26415117478346Chembridge (Drug-like library)255.311683.1561260S ribosome export