3470-5649

3-[(3-fluorophenyl)methylamino]-2-methylquinazolin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1393
Screen concentration 58.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 4108395
SMILES CC1=NC2=CC=CC=C2C(=O)N1NCC3=CC(=CC=C3)F
Standardized SMILES CC1=Nc2ccccc2C(=O)N1NCc3cccc(F)c3
Molecular weight 283.3003
ALogP 2.33
H-bond donor count 1
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.7
% growth inhibition (Hom. pool) 5.88


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4108395
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.14||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ALG2(YGL065C)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.12||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:CBF5(YLR175W)|FD-Score:4.32|P-value:7.96E-6|Clearance:0.15||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:LSG1(YGL099W)|FD-Score:6.81|P-value:4.84E-12|Clearance:2.5||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS1(YLR163C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NMD3(YHR170W)|FD-Score:3.99|P-value:3.24E-5|Clearance:0.46||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRE5(YMR314W)|FD-Score:-3.34|P-value:4.14E-4|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.17|P-value:7.74E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMD1(YGR074W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.17||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:-3.59|P-value:1.64E-4|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:YJL195C(YJL195C_d)|FD-Score:3.11|P-value:9.34E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:ADE13(YLR359W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.14||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ALG2(YGL065C)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.12||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:CBF5(YLR175W)|FD-Score:4.32|P-value:7.96E-6|Clearance:0.15||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:LSG1(YGL099W)|FD-Score:6.81|P-value:4.84E-12|Clearance:2.5||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS1(YLR163C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NMD3(YHR170W)|FD-Score:3.99|P-value:3.24E-5|Clearance:0.46||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRE5(YMR314W)|FD-Score:-3.34|P-value:4.14E-4|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.17|P-value:7.74E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMD1(YGR074W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.17||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:-3.59|P-value:1.64E-4|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:YJL195C(YJL195C_d)|FD-Score:3.11|P-value:9.34E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4108395
Download HOP data (tab-delimited text)  (excel)
Gene:ADE3(YGR204W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ALY1(YKR021W)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ASM4(YDL088C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:CBF1(YJR060W)|FD-Score:-14.3|P-value:2.24E-46||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COG7(YGL005C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:4.36|P-value:6.37E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSS4(YPR017C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:DYN3(YMR299C)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FCY1(YPR062W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FMT1(YBL013W)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GID8(YMR135C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:HBS1(YKR084C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HUA2(YOR284W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:LDB16(YCL005W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LGE1(YPL055C)|FD-Score:4.98|P-value:3.16E-7||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAC1(YMR021C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL32(YBR299W)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCH4(YOL119C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MLC2(YPR188C)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MRM1(YOR201C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSN1(YOL116W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MUP1(YGR055W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:PET191(YJR034W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PTC6(YCR079W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:SER2(YGR208W)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SHE1(YBL031W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIM1(YIL123W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SIS2(YKR072C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKI3(YPR189W)|FD-Score:5.39|P-value:3.50E-8||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKN7(YHR206W)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SSA2(YLL024C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STD1(YOR047C)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TRP1(YDR007W)|FD-Score:5.77|P-value:4.00E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.26|P-value:1.97E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:WHI3(YNL197C)|FD-Score:-4.3|P-value:8.61E-6||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:6.35|P-value:1.10E-10||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAF9(YNL107W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YDR340W(YDR340W_d)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLR211C(YLR211C_p)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR444C(YLR444C_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Putative protein of unknown function Gene:YMR090W(YMR090W_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR130W(YMR130W_p)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YNL303W(YNL303W_d)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR076W(YPR076W_d)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE3(YGR204W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ALY1(YKR021W)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ASM4(YDL088C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:CBF1(YJR060W)|FD-Score:-14.3|P-value:2.24E-46||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COG7(YGL005C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:4.36|P-value:6.37E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSS4(YPR017C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:DYN3(YMR299C)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FCY1(YPR062W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FMT1(YBL013W)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GID8(YMR135C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:HBS1(YKR084C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HUA2(YOR284W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:LDB16(YCL005W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LGE1(YPL055C)|FD-Score:4.98|P-value:3.16E-7||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAC1(YMR021C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL32(YBR299W)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCH4(YOL119C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MLC2(YPR188C)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MRM1(YOR201C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSN1(YOL116W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MUP1(YGR055W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:PET191(YJR034W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PTC6(YCR079W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:SER2(YGR208W)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SHE1(YBL031W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIM1(YIL123W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SIS2(YKR072C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKI3(YPR189W)|FD-Score:5.39|P-value:3.50E-8||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKN7(YHR206W)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SSA2(YLL024C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STD1(YOR047C)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TRP1(YDR007W)|FD-Score:5.77|P-value:4.00E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.26|P-value:1.97E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:WHI3(YNL197C)|FD-Score:-4.3|P-value:8.61E-6||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:6.35|P-value:1.10E-10||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAF9(YNL107W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YDR340W(YDR340W_d)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLR211C(YLR211C_p)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR444C(YLR444C_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Putative protein of unknown function Gene:YMR090W(YMR090W_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR130W(YMR130W_p)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YNL303W(YNL303W_d)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR076W(YPR076W_d)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W6.814.84E-122.50LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YLR175W4.327.96E-60.15CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YHR062C4.171.54E-50.17RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR170W3.993.24E-50.46NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YLR359W3.532.06E-40.14ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YGR074W3.403.43E-40.17SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YGL065C3.236.19E-40.12ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YJL195C_d3.119.34E-40.03YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YOR075W3.080.001030.17UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YOR020C2.910.001810.15HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YGR083C2.760.002920.08GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YPL160W2.680.003710.03CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YNR053C2.640.004120.04NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YML130C2.610.004590.03ERO1Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
YKL111C_d2.580.004950.01YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL283C6.351.10E-10WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YER090W6.261.97E-10TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR007W5.774.00E-9TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YPR189W5.393.50E-8SKI3Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YPL055C4.983.16E-7LGE1Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
YPR188C4.661.57E-6MLC2Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
YLR239C4.493.54E-6LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YIR023W4.366.37E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YBL013W4.337.50E-6FMT1Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
YKR021W4.318.16E-6ALY1Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
YCL005W4.241.10E-5LDB16Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
YKR084C4.211.28E-5HBS1GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay
YFR036W4.201.31E-5CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YOL119C4.191.38E-5MCH4Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
YNL107W3.914.66E-5YAF9Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain

GO enrichment analysis for SGTC_1393
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1805.85E-44SGTC_13833049-0032 20.7 μMChemDiv (Drug-like library)7128640.14705960S ribosome export
0.1701.63E-39SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.102941
0.1631.35E-36SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.114286
0.1541.74E-32SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.12820560S ribosome export
0.1526.58E-32SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.0958904amide catabolism
0.1491.34E-30SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.108434
0.1481.84E-30SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.095890460S ribosome export
0.1479.97E-30SGTC_1733st037357 54.9 μMTimTec (Natural product derivative library)12723390.21917860S ribosome export
0.1381.70E-26SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.11428660S ribosome export
0.1372.69E-26SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.098901160S ribosome export
0.1338.07E-25SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.08
0.1323.23E-24SGTC_11811222-0449 220.0 μMChemDiv (Drug-like library)6583180.134328
0.1301.15E-23SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.162162plasma membrane duress
0.1286.71E-23SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.11940360S ribosome export
0.1287.35E-23SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0326087

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13853091-483414.1 μM0.3684214072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion
SGTC_21685727979200 μM0.339623695210Chembridge (Fragment library)206.26422.23603
SGTC_1734st03716869.4 μM0.323077708907TimTec (Natural product derivative library)288.2770231.68904
SGTC_3393091-483371.4 μM0.3220343240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_1469k284-2957126 μM0.30882423884841ChemDiv (Drug-like library)387.253744.88314
SGTC_13993562-577225 μM0.29411823847490ChemDiv (Drug-like library)394.7829834.86305RPP1 & pyrimidine depletion
SGTC_21775811945192 μM0.2931035343475Chembridge (Fragment library)201.224540.91212
SGTC_5931203-021861.7 μM0.2881363666956ChemDiv (Drug-like library)244.33213.65302
SGTC_21785812956200 μM0.2881365403383Chembridge (Fragment library)215.251121.49212
SGTC_3084911687749.47 μM0.28571427241325Chembridge (Drug-like library)385.28814.07205