3474-0001

ethyl-(6-methyl-2,3,4,9-tetrahydro-1H-carbazol-1-yl)azanium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1394
Screen concentration 65.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 5017666
SMILES CC[NH2+]C1CCCC2=C1NC3=C2C=C(C=C3)C
Standardized SMILES CC[NH2+]C1CCCc2c1[nH]c3ccc(C)cc23
Molecular weight 229.3406
ALogP 2.47
H-bond donor count 2
H-bond acceptor count 0
Response signature endomembrane recycling

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.53
% growth inhibition (Hom. pool) 1.32


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5017666
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:MTW1(YAL034W-A)|FD-Score:3.83|P-value:6.45E-5|Clearance:0.28||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NEO1(YIL048W)|FD-Score:25.9|P-value:2.72E-148|Clearance:21.2||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:RHO1(YPR165W)|FD-Score:4.7|P-value:1.28E-6|Clearance:0.66||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB8(YOR224C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN3(YER021W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.28||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:SEC13(YLR208W)|FD-Score:-3.22|P-value:6.41E-4|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC26(YDR238C)|FD-Score:3.88|P-value:5.16E-5|Clearance:0.05||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMD1(YGR074W)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.16||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:ARP9(YMR033W)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:MTW1(YAL034W-A)|FD-Score:3.83|P-value:6.45E-5|Clearance:0.28||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NEO1(YIL048W)|FD-Score:25.9|P-value:2.72E-148|Clearance:21.2||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:RHO1(YPR165W)|FD-Score:4.7|P-value:1.28E-6|Clearance:0.66||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB8(YOR224C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN3(YER021W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.28||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:SEC13(YLR208W)|FD-Score:-3.22|P-value:6.41E-4|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC26(YDR238C)|FD-Score:3.88|P-value:5.16E-5|Clearance:0.05||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMD1(YGR074W)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.16||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5017666
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:7.56|P-value:2.01E-14||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM32(YML050W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ATO3(YDR384C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AVT1(YJR001W)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI1(YNL271C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:8.13|P-value:2.09E-16||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD26(YDR241W_d)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBF1(YJR060W)|FD-Score:7.43|P-value:5.46E-14||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CCP1(YKR066C)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CDC50(YCR094W)|FD-Score:7.37|P-value:8.31E-14||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDC73(YLR418C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CKB2(YOR039W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNE1(YAL058W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CTF18(YMR078C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTK3(YML112W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYS3(YAL012W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:END3(YNL084C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERI1(YPL096C-A)|FD-Score:5.1|P-value:1.74E-7||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV14(YGL054C)|FD-Score:6.06|P-value:6.95E-10||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMP10(YER182W_p)|FD-Score:4.36|P-value:6.62E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HSC82(YMR186W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:HXT4(YHR092C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:IRC5(YFR038W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:LOA1(YPR139C)|FD-Score:6.29|P-value:1.55E-10||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MOT2(YER068W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPL4(YLR439W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MUP1(YGR055W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT3(YPR131C)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NHX1(YDR456W)|FD-Score:7.17|P-value:3.63E-13||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NMA111(YNL123W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP84(YDL116W)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:ODC2(YOR222W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PEP12(YOR036W)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET111(YMR257C)|FD-Score:5.23|P-value:8.40E-8||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PIN2(YOR104W)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PMP3(YDR276C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PMT7(YDR307W_p)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RAM1(YDL090C)|FD-Score:5.84|P-value:2.54E-9||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:33.6|P-value:3.52E-247||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM9(YMR063W)|FD-Score:5.36|P-value:4.11E-8||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPN4(YDL020C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:5.25|P-value:7.75E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:10.2|P-value:1.11E-24||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCO1(YBR037C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SHE1(YBL031W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHE4(YOR035C)|FD-Score:8.07|P-value:3.63E-16||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLZ1(YNL196C_p)|FD-Score:4.34|P-value:7.26E-6||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SMI1(YGR229C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF7(YLR025W)|FD-Score:4.76|P-value:9.74E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:5.78|P-value:3.84E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOV1(YMR066W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPF1(YEL031W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPS18(YNL204C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPT2(YER161C)|FD-Score:-4.53|P-value:2.92E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SUC2(YIL162W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SWA2(YDR320C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWT21(YNL187W)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TAE2(YPL009C)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TLG2(YOL018C)|FD-Score:4.52|P-value:3.13E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:4.78|P-value:8.73E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM9(YML014W)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP2(YER090W)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS1(YKR001C)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL094C(YBL094C_d)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR184W(YBR184W_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR200W-A(YBR200W-A_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR134C(YDR134C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YDR455C(YDR455C_d)|FD-Score:8.68|P-value:1.92E-18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YKR073C(YKR073C_d)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR104W(YKR104W)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLL007C(YLL007C_p)|FD-Score:4.3|P-value:8.43E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR455W(YLR455W_p)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YPL191C(YPL191C_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR003C(YPR003C_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR053C(YPR053C_d)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR076W(YPR076W_d)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YSC83(YHR017W)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YVH1(YIR026C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:AFT1(YGL071W)|FD-Score:7.56|P-value:2.01E-14||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM32(YML050W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ATO3(YDR384C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AVT1(YJR001W)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI1(YNL271C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:8.13|P-value:2.09E-16||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD26(YDR241W_d)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBF1(YJR060W)|FD-Score:7.43|P-value:5.46E-14||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CCP1(YKR066C)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CDC50(YCR094W)|FD-Score:7.37|P-value:8.31E-14||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDC73(YLR418C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CKB2(YOR039W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNE1(YAL058W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CTF18(YMR078C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTK3(YML112W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYS3(YAL012W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:END3(YNL084C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERI1(YPL096C-A)|FD-Score:5.1|P-value:1.74E-7||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV14(YGL054C)|FD-Score:6.06|P-value:6.95E-10||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMP10(YER182W_p)|FD-Score:4.36|P-value:6.62E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HSC82(YMR186W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:HXT4(YHR092C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:IRC5(YFR038W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:LOA1(YPR139C)|FD-Score:6.29|P-value:1.55E-10||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MOT2(YER068W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPL4(YLR439W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MUP1(YGR055W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT3(YPR131C)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NHX1(YDR456W)|FD-Score:7.17|P-value:3.63E-13||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NMA111(YNL123W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP84(YDL116W)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:ODC2(YOR222W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PEP12(YOR036W)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET111(YMR257C)|FD-Score:5.23|P-value:8.40E-8||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PIN2(YOR104W)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PMP3(YDR276C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PMT7(YDR307W_p)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RAM1(YDL090C)|FD-Score:5.84|P-value:2.54E-9||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:33.6|P-value:3.52E-247||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM9(YMR063W)|FD-Score:5.36|P-value:4.11E-8||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPN4(YDL020C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:5.25|P-value:7.75E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:10.2|P-value:1.11E-24||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCO1(YBR037C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SHE1(YBL031W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHE4(YOR035C)|FD-Score:8.07|P-value:3.63E-16||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLZ1(YNL196C_p)|FD-Score:4.34|P-value:7.26E-6||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SMI1(YGR229C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF7(YLR025W)|FD-Score:4.76|P-value:9.74E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:5.78|P-value:3.84E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOV1(YMR066W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPF1(YEL031W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPS18(YNL204C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPT2(YER161C)|FD-Score:-4.53|P-value:2.92E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SUC2(YIL162W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SWA2(YDR320C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWT21(YNL187W)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TAE2(YPL009C)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TLG2(YOL018C)|FD-Score:4.52|P-value:3.13E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:4.78|P-value:8.73E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM9(YML014W)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP2(YER090W)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS1(YKR001C)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL094C(YBL094C_d)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR184W(YBR184W_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR200W-A(YBR200W-A_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR134C(YDR134C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YDR455C(YDR455C_d)|FD-Score:8.68|P-value:1.92E-18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YKR073C(YKR073C_d)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR104W(YKR104W)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLL007C(YLL007C_p)|FD-Score:4.3|P-value:8.43E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR455W(YLR455W_p)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YPL191C(YPL191C_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR003C(YPR003C_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR053C(YPR053C_d)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR076W(YPR076W_d)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YSC83(YHR017W)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YVH1(YIR026C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W25.902.72E-14821.20NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YPR165W4.701.28E-60.66RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YGR074W4.042.68E-50.16SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YDR238C3.885.16E-50.05SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YAL034W-A3.836.45E-50.28MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YER021W3.551.93E-40.28RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YOR224C3.275.42E-40.06RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YMR033W3.216.63E-40.13ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR197W3.080.001020.06NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDL153C3.020.001270.28SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YMR203W2.740.003110.05TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YLR409C2.680.003650.06UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YDR145W2.630.004310.03TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YLR163C2.600.004710.10MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YNR016C2.500.006230.04ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C33.603.52E-247RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDR129C10.201.11E-24SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YDR455C_d8.681.92E-18YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YPL069C8.132.09E-16BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YOR035C8.073.63E-16SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YGL071W7.562.01E-14AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YJR060W7.435.46E-14CBF1Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
YCR094W7.378.31E-14CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YGL095C7.331.18E-13VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YDR456W7.173.63E-13NHX1Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
YMR202W6.311.40E-10ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YPR139C6.291.55E-10LOA1Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
YGL054C6.066.95E-10ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YER090W5.901.79E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDL090C5.842.54E-9RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit

GO enrichment analysis for SGTC_1394
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6210SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.117647NEO1
0.5590SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.117647NEO1
0.5120SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.102273NEO1
0.5080SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.107692NEO1
0.4960SGTC_232clozapine 119.0 μMMiscellaneous28180.0810811NEO1
0.4710SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.115942NEO1
0.4640SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.134328NEO1
0.4582.23E-304SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.0625NEO1
0.4553.46E-299SGTC_270tamoxifen 5.5 μMMiscellaneous53760.0882353NEO1
0.4351.23E-270SGTC_11303454-2858 15.9 μMChemDiv (Drug-like library)7156280.084507NEO1
0.4312.28E-266SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.102941NEO1
0.4303.25E-264SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.105263NEO1
0.4156.70E-245SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.121622NEO1
0.3932.09E-217SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.133333NEO1-PIK1
0.3933.22E-217SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.08endomembrane recycling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2694st07768581.9 μM0.5576922838096TimTec (Natural product derivative library)244.33212.80832endomembrane recycling
SGTC_1670st0165954.04 μM0.431373597879TimTec (Natural product derivative library)265.149023.17821
SGTC_1720st0372989.48 μM0.431373597396TimTec (Natural product derivative library)266.133783.46721
SGTC_13291447-169750.6 μM0.3906252840515ChemDiv (Drug-like library)286.325722.57623
SGTC_2696st07769174.32 μM0.384615757133TimTec (Natural product derivative library)200.279542.83611
SGTC_1885st05754699.9 μM0.377358702008TimTec (Natural product derivative library)200.236482.18321
SGTC_1952st07703664.1 μM0.3606562937007TimTec (Natural product derivative library)258.315623.01412
SGTC_2698st07769767.5 μM0.3181823154499TimTec (Natural product derivative library)296.406664.12322
SGTC_1671st01659674.5 μM0.2923082829167TimTec (Natural product derivative library)268.35353.16701
SGTC_1673st01661127.3 μM0.2878795226799TimTec (Natural product derivative library)271.314385.50803