3909-7815

2-(3,4-dimethoxyphenyl)-N-[(4-fluorophenyl)methyl]quinazolin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1403
Screen concentration 62.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 6615483
SMILES COC1=C(C=C(C=C1)C2=NC3=CC=CC=C3C(=N2)NCC4=CC=C(C=C4)F)OC
Standardized SMILES COc1ccc(cc1OC)c2nc(NCc3ccc(F)cc3)c4ccccc4n2
Molecular weight 389.4222
ALogP 5.11
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.42
% growth inhibition (Hom. pool) 6.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6615483
Download HIP data (tab-delimited text)  (excel)
Gene:FCP1(YMR277W)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.15||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GCD1(YOR260W)|FD-Score:-3.1|P-value:9.83E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:3.92|P-value:4.50E-5|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM4(YOR278W)|FD-Score:3.19|P-value:7.05E-4|Clearance:0.14||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:IPI1(YHR085W)|FD-Score:4.41|P-value:5.15E-6|Clearance:0.21||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:LSG1(YGL099W)|FD-Score:6.37|P-value:9.53E-11|Clearance:1.74||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MET30(YIL046W)|FD-Score:3.93|P-value:4.18E-5|Clearance:0.02||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NMD3(YHR170W)|FD-Score:3.83|P-value:6.37E-5|Clearance:0.08||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPS2(YGL123W)|FD-Score:-3.94|P-value:4.00E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEC10(YLR166C)|FD-Score:4.63|P-value:1.86E-6|Clearance:0.22||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SPT14(YPL175W)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.27||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRP1(YNL189W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.4||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:FCP1(YMR277W)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.15||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GCD1(YOR260W)|FD-Score:-3.1|P-value:9.83E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:3.92|P-value:4.50E-5|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM4(YOR278W)|FD-Score:3.19|P-value:7.05E-4|Clearance:0.14||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:IPI1(YHR085W)|FD-Score:4.41|P-value:5.15E-6|Clearance:0.21||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:LSG1(YGL099W)|FD-Score:6.37|P-value:9.53E-11|Clearance:1.74||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MET30(YIL046W)|FD-Score:3.93|P-value:4.18E-5|Clearance:0.02||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NMD3(YHR170W)|FD-Score:3.83|P-value:6.37E-5|Clearance:0.08||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPS2(YGL123W)|FD-Score:-3.94|P-value:4.00E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEC10(YLR166C)|FD-Score:4.63|P-value:1.86E-6|Clearance:0.22||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SPT14(YPL175W)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.27||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRP1(YNL189W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.4||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6615483
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:6.33|P-value:1.22E-10||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:APM3(YBR288C)|FD-Score:10.4|P-value:1.94E-25||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:AZR1(YGR224W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BNI1(YNL271C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BST1(YFL025C)|FD-Score:4.79|P-value:8.20E-7||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD27(YFL023W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAP1(YKL007W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:-4.6|P-value:2.14E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCR4(YAL021C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CDC50(YCR094W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKA2(YOR061W)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG7(YGL005C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GLC8(YMR311C)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GTT3(YEL017W)|FD-Score:5.34|P-value:4.59E-8||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HAP5(YOR358W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HBS1(YKR084C)|FD-Score:4.41|P-value:5.22E-6||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HPR1(YDR138W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSP26(YBR072W)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HXT14(YNL318C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HXT8(YJL214W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:KRE1(YNL322C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:MCK1(YNL307C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM38(YOL027C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MUB1(YMR100W)|FD-Score:-5.4|P-value:3.32E-8||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:PDR1(YGL013C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR18(YNR070W_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PHO80(YOL001W)|FD-Score:7.66|P-value:9.01E-15||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:REG1(YDR028C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPN4(YDL020C)|FD-Score:8.33|P-value:4.13E-17||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SIM1(YIL123W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SNQ2(YDR011W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPT2(YER161C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SUR4(YLR372W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.83|P-value:4.26E-23||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR444W(YDR444W_p)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGR125W(YGR125W_p)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJR120W(YJR120W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p Gene:YLR072W(YLR072W_p)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YML082W(YML082W_p)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR160W(YMR160W_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YOL029C(YOL029C_p)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOR111W(YOR111W_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function Gene:YPR015C(YPR015C_p)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:ACE2(YLR131C)|FD-Score:6.33|P-value:1.22E-10||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:APM3(YBR288C)|FD-Score:10.4|P-value:1.94E-25||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:AZR1(YGR224W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BNI1(YNL271C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BST1(YFL025C)|FD-Score:4.79|P-value:8.20E-7||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD27(YFL023W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAP1(YKL007W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:-4.6|P-value:2.14E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCR4(YAL021C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CDC50(YCR094W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKA2(YOR061W)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG7(YGL005C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GLC8(YMR311C)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GTT3(YEL017W)|FD-Score:5.34|P-value:4.59E-8||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HAP5(YOR358W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HBS1(YKR084C)|FD-Score:4.41|P-value:5.22E-6||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HPR1(YDR138W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSP26(YBR072W)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HXT14(YNL318C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HXT8(YJL214W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:KRE1(YNL322C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:MCK1(YNL307C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM38(YOL027C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MUB1(YMR100W)|FD-Score:-5.4|P-value:3.32E-8||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:PDR1(YGL013C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR18(YNR070W_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PHO80(YOL001W)|FD-Score:7.66|P-value:9.01E-15||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:REG1(YDR028C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPN4(YDL020C)|FD-Score:8.33|P-value:4.13E-17||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SIM1(YIL123W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SNQ2(YDR011W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPT2(YER161C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SUR4(YLR372W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.83|P-value:4.26E-23||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR444W(YDR444W_p)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGR125W(YGR125W_p)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJR120W(YJR120W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p Gene:YLR072W(YLR072W_p)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YML082W(YML082W_p)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR160W(YMR160W_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YOL029C(YOL029C_p)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOR111W(YOR111W_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function Gene:YPR015C(YPR015C_p)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W6.379.53E-111.74LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YLR166C4.631.86E-60.22SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YHR085W4.415.15E-60.21IPI1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
YPL175W4.201.33E-50.27SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YIL046W3.934.18E-50.02MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YER133W3.924.50E-50.08GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YHR170W3.836.37E-50.08NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YNL189W3.758.89E-50.40SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YMR277W3.354.08E-40.15FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YOR278W3.197.05E-40.14HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YJR057W3.050.001140.06CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YER003C2.990.001410.22PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YLR223C2.760.002860.04IFH1Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
YDR327W_d2.720.003260.01YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YGR216C2.710.003380.10GPI1Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR288C10.401.94E-25APM3Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
YBR058C-A9.834.26E-23TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDL020C8.334.13E-17RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YOL001W7.669.01E-15PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
YLR131C6.331.22E-10ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YEL017W5.344.59E-8GTT3Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
YOR061W4.993.06E-7CKA2Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
YBR072W4.943.87E-7HSP26Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
YFL025C4.798.20E-7BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YLR072W_p4.434.80E-6YLR072W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene
YKR084C4.415.22E-6HBS1GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay
YGR125W_p4.395.57E-6YGR125W_pPutative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YML071C4.201.31E-5COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL013C4.201.31E-5PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YML082W_p4.102.07E-5YML082W_pPutative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene

GO enrichment analysis for SGTC_1403
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3361.53E-155SGTC_2740felodipine 17.1 μMMiscellaneous33330.097561
0.2911.34E-115SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0724638
0.2902.13E-114SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.176471TSC3-RPN4
0.2631.63E-93SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0649351
0.2464.62E-82SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0704225
0.2466.49E-82SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0704225
0.2461.15E-81SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.126761TSC3-RPN4
0.2442.24E-80SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.125TSC3-RPN4
0.2432.82E-80SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0779221TSC3-RPN4
0.2372.70E-76SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.091954TSC3-RPN4
0.2358.50E-75SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.07PDR1
0.2342.81E-74SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0263158TSC3-RPN4
0.2272.25E-69SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0731707TSC3-RPN4
0.2234.64E-67SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0704225TSC3-RPN4
0.2221.61E-66SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.13253

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14043909-782048.3 μM0.57894723741373ChemDiv (Drug-like library)371.431745.551560S ribosome export
SGTC_4253825-701713.1 μM0.4666673397440ChemDiv (Drug-like library)329.3702435.78914
SGTC_14053909-789412 μM0.4576277104060ChemDiv (Drug-like library)293.362963.7111460S ribosome export
SGTC_14063909-790611.9 μM0.442623X1406ChemDiv (Drug-like library)307.389543.7734160S ribosome export
SGTC_3287911717337.06 μM0.4406782789709Chembridge (Drug-like library)329.82393.6692460S ribosome export
SGTC_8103825-7150143 μM0.43076922330411ChemDiv (Drug-like library)415.48436.021660S ribosome export
SGTC_14073909-790912.3 μM0.3676474411780ChemDiv (Drug-like library)319.400244.2011460S ribosome export
SGTC_1983454-287316.19 μM0.366667710957ChemDiv (Drug-like library)297.782044.7461360S ribosome export
SGTC_14023909-767335.9 μM0.3559325328014ChemDiv (Drug-like library)269.728883.98313
SGTC_558k064-002721.3 μM0.338715088526ChemDiv (Drug-like library)334.79895.55113DNA damage response