3909-8106

N-[2-(3,4-dimethoxyphenyl)ethyl]-2-methyl-5,6,7,8-tetrahydro-[1]benzothiolo[2,3-d]pyrimidin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1410
Screen concentration 77.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 1039210
SMILES CC1=NC(=C2C3=C(CCCC3)SC2=N1)NCCC4=CC(=C(C=C4)OC)OC
Standardized SMILES COc1ccc(CCNc2nc(C)nc3sc4CCCCc4c23)cc1OC
Molecular weight 383.5071
ALogP 4.67
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.4
% growth inhibition (Hom. pool) 8.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1039210
Download HIP data (tab-delimited text)  (excel)
Gene:CFT2(YLR115W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.09||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:DSN1(YIR010W)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.05||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FAS2(YPL231W)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:LST8(YNL006W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.13||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:PTR3(YFR029W)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.09||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RLI1(YDR091C)|FD-Score:-3.19|P-value:7.15E-4|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:ROK1(YGL171W)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.12||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RRP42(YDL111C)|FD-Score:-3.3|P-value:4.75E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:STS1(YIR011C)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.5||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:CFT2(YLR115W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.09||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:DSN1(YIR010W)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.05||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FAS2(YPL231W)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:LST8(YNL006W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.13||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:PTR3(YFR029W)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.09||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RLI1(YDR091C)|FD-Score:-3.19|P-value:7.15E-4|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:ROK1(YGL171W)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.12||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RRP42(YDL111C)|FD-Score:-3.3|P-value:4.75E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:STS1(YIR011C)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.5||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1039210
Download HOP data (tab-delimited text)  (excel)
Gene:ATG11(YPR049C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG13(YPR185W)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:BCK2(YER167W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:EFG1(YGR271C-A)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FYV6(YNL133C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GLC8(YMR311C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPB2(YAL056W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:IDP1(YDL066W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRS4(YKR019C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JHD1(YER051W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:LYS14(YDR034C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MRPL32(YCR003W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NGG1(YDR176W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PBS2(YJL128C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCL2(YDL127W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Gene:RCN2(YOR220W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RLF2(YPR018W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPS14B(YJL191W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RRT8(YOL048C_p)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress Gene:SCP1(YOR367W)|FD-Score:4.95|P-value:3.79E-7||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SET3(YKR029C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SRB8(YCR081W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSD1(YDR293C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:8.61|P-value:3.67E-18||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TRP1(YDR007W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:5.01|P-value:2.77E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS21(YOR089C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS28(YPL065W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YBR099C(YBR099C_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YEL1(YBL060W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YLF2(YHL014C)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR460C(YLR460C_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML122C(YML122C_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL095C(YNL095C_p)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNR014W(YNR014W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR065C(YNR065C_p)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:8.63|P-value:3.00E-18||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL162C(YPL162C_p)|FD-Score:4.76|P-value:9.59E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:ATG11(YPR049C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG13(YPR185W)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:BCK2(YER167W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:EFG1(YGR271C-A)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FYV6(YNL133C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GLC8(YMR311C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPB2(YAL056W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:IDP1(YDL066W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRS4(YKR019C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JHD1(YER051W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:LYS14(YDR034C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MRPL32(YCR003W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NGG1(YDR176W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PBS2(YJL128C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCL2(YDL127W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Gene:RCN2(YOR220W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RLF2(YPR018W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPS14B(YJL191W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RRT8(YOL048C_p)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress Gene:SCP1(YOR367W)|FD-Score:4.95|P-value:3.79E-7||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SET3(YKR029C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SRB8(YCR081W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSD1(YDR293C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:8.61|P-value:3.67E-18||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TRP1(YDR007W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:5.01|P-value:2.77E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS21(YOR089C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS28(YPL065W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YBR099C(YBR099C_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YEL1(YBL060W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YLF2(YHL014C)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR460C(YLR460C_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML122C(YML122C_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL095C(YNL095C_p)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNR014W(YNR014W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR065C(YNR065C_p)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:8.63|P-value:3.00E-18||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL162C(YPL162C_p)|FD-Score:4.76|P-value:9.59E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIR011C4.013.04E-50.50STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YLR115W3.512.27E-40.09CFT2Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
YGL171W3.423.11E-40.12ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YIR010W3.304.80E-40.05DSN1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
YNL006W3.255.78E-40.13LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YFR029W3.129.14E-40.09PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YDR047W3.030.001230.05HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YFL017C2.980.001450.07GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YOL094C2.910.001800.02RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR168W2.890.001920.02GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YIL083C2.870.002050.04CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YKL024C2.830.002300.05URA6Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
YPR169W2.780.002720.06JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YDL150W2.720.003272.97E-4RPC53RNA polymerase III subunit C53
YER133W2.720.003270.12GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W8.633.00E-18YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR372W8.613.67E-18SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YPR185W5.658.12E-9ATG13Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling
YBR058C-A5.012.77E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOR367W4.953.79E-7SCP1Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin
YPL162C_p4.769.59E-7YPL162C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
YDR176W4.621.91E-6NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YKR019C4.111.98E-5IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YPR049C4.072.34E-5ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)
YGR271C-A4.072.36E-5EFG1Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
YOL048C_p4.042.69E-5RRT8_pProtein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress
YDR007W4.022.97E-5TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YHL014C3.807.28E-5YLF2Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YCR081W3.502.33E-4SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YBR099C_d3.502.35E-4YBR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4

GO enrichment analysis for SGTC_1410
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3081.28E-129SGTC_2740felodipine 17.1 μMMiscellaneous33330.0930233
0.2748.95E-102SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0808081
0.2441.57E-80SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.0632911
0.2361.04E-75SGTC_1982st072467 71.9 μMTimTec (Natural product derivative library)31635330.047619
0.2358.38E-75SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.333333
0.2301.15E-71SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.0842105
0.2193.28E-65SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.05TSC3-RPN4
0.1904.47E-49SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.109756
0.1871.16E-47SGTC_2627acetyl isogambogic acid 100.0 μMMicrosource (Natural product library)68577890.0472441TSC3-RPN4
0.1845.70E-46SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0933333TSC3-RPN4
0.1831.38E-45SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0133333
0.1791.12E-43SGTC_1545lovastatin 49.4 μMTimTec (Pure natural product library)129236120.0571429
0.1782.61E-43SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.147727
0.1763.96E-42SGTC_1573oleylamine 1.1 μMTimTec (Pure natural product library)53567890.0294118
0.1738.89E-41SGTC_487nimodipine 119.0 μMMiscellaneous44970.0947368

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_14083909-797648.8 μM0.5238124152374ChemDiv (Drug-like library)315.456284.12215Golgi
SGTC_8720588-0139234 μM0.3692312047100ChemDiv (Drug-like library)289.395862.8405
SGTC_11503909-798310.9 μM0.35211323965914ChemDiv (Drug-like library)327.400783.39816
SGTC_1472k841-0093193 μM0.342857X1472ChemDiv (Drug-like library)345.459113.21750
SGTC_11333473-024750 μM0.338028711311ChemDiv (Drug-like library)308.422343.35625RSC complex & mRNA processing
SGTC_12273909-801046.6 μM0.33333315982272ChemDiv (Drug-like library)249.375063.37614
SGTC_14113909-8223150 μM0.333333X1411ChemDiv (Drug-like library)468.656176.19241
SGTC_24036032726181.2 μM0.3076922881040Miscellaneous265.39763.80615
SGTC_8184072-270753.5 μM0.306452882871ChemDiv (Drug-like library)272.40844.35313
SGTC_14093909-800461.9 μM0.32119279ChemDiv (Drug-like library)273.35342.81215