3909-8563

3-[2-(diethylamino)-2-oxoethyl]-5-methyl-4-oxo-N-quinolin-3-ylthieno[2,3-d]pyrimidine-6-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1412
Screen concentration 178.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 23767530
SMILES CCN(CC)C(=O)CN1C=NC2=C(C1=O)C(=C(S2)C(=O)NC3=CC4=CC=CC=C4N=C3)C
Standardized SMILES CCN(CC)C(=O)CN1C=Nc2sc(C(=O)Nc3cnc4ccccc4c3)c(C)c2C1=O
Molecular weight 449.5254
ALogP 2.45
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.14
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 23767530
Download HIP data (tab-delimited text)  (excel)
Gene:CDC42(YLR229C)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.08||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FHL1(YPR104C)|FD-Score:3.79|P-value:7.59E-5|Clearance:0.68||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:YIF1(YNL263C)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:CDC42(YLR229C)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.08||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FHL1(YPR104C)|FD-Score:3.79|P-value:7.59E-5|Clearance:0.68||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:YIF1(YNL263C)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 23767530
Download HOP data (tab-delimited text)  (excel)
Gene:ADH2(YMR303C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AHA1(YDR214W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AMS1(YGL156W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:APL4(YPR029C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:CMC1(YKL137W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:ENT2(YLR206W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ERP3(YDL018C)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR11(YNL127W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FHN1(YGR131W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:GNP1(YDR508C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GSY1(YFR015C)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GUP1(YGL084C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:ILM1(YJR118C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INP51(YIL002C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:ITT1(YML068W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:IZH1(YDR492W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:KEX1(YGL203C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KNH1(YDL049C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LYS4(YDR234W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MCM21(YDR318W)|FD-Score:4.91|P-value:4.50E-7||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:OSH2(YDL019C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PER1(YCR044C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX18(YHR160C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PSP1(YDR505C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:SOP4(YJL192C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRF1(YDL133W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSA1(YAL005C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:TVP15(YDR100W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBP5(YER144C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:YFL042C(YFL042C_p)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL242C(YGL242C_p)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YJU3(YKL094W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR282C(YLR282C_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL296W(YNL296W_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL150C(YOL150C_d)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADH2(YMR303C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AHA1(YDR214W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AMS1(YGL156W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:APL4(YPR029C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:CMC1(YKL137W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:ENT2(YLR206W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ERP3(YDL018C)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR11(YNL127W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FHN1(YGR131W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:GNP1(YDR508C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GSY1(YFR015C)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GUP1(YGL084C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:ILM1(YJR118C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INP51(YIL002C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:ITT1(YML068W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:IZH1(YDR492W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:KEX1(YGL203C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KNH1(YDL049C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LYS4(YDR234W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MCM21(YDR318W)|FD-Score:4.91|P-value:4.50E-7||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:OSH2(YDL019C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PER1(YCR044C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX18(YHR160C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PSP1(YDR505C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:SOP4(YJL192C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRF1(YDL133W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSA1(YAL005C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:TVP15(YDR100W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBP5(YER144C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:YFL042C(YFL042C_p)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL242C(YGL242C_p)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YJU3(YKL094W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR282C(YLR282C_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL296W(YNL296W_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL150C(YOL150C_d)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR104C3.797.59E-50.68FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
YLR229C3.119.50E-40.08CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YLR026C3.030.001240.09SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YGL011C2.940.001650.21SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YBR038W2.730.003150.01CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YPR108W2.720.003260.03RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YER009W2.690.003600.03NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YFL008W2.650.003970.00SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YGR246C2.650.004010.02BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YLR310C2.630.004280.08CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YGL111W2.540.005470.02NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YGL207W2.530.005720.05SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YGL245W2.480.006570.02GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YOL026C2.460.006930.04MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YDL029W2.420.007820.06ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR318W4.914.50E-7MCM21Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
YLL010C4.631.79E-6PSR1Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
YGL242C_p4.405.53E-6YGL242C_pPutative protein of unknown function; deletion mutant is viable
YFL042C_p4.385.88E-6YFL042C_pPutative protein of unknown function; YFL042C is not an essential gene
YPR029C4.151.68E-5APL4Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
YFR015C3.993.29E-5GSY1Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YER144C3.944.11E-5UBP5Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck
YDL018C3.934.25E-5ERP3Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
YJL192C3.924.41E-5SOP4ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER
YDR214W3.846.16E-5AHA1Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
YOL150C_d3.768.53E-5YOL150C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML068W3.701.08E-4ITT1Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
YGL203C3.691.11E-4KEX1Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
YKL068W-A_p3.681.19E-4YKL068W-A_pPutative protein of unknown function; identified by homology to Ashbya gossypii
YLR282C_d3.561.84E-4YLR282C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition

GO enrichment analysis for SGTC_1412
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2177.38E-64SGTC_14314049-0260 9.1 μMChemDiv (Drug-like library)38548410.0967742
0.1963.76E-52SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.0842105
0.1062.74E-16SGTC_25422-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 100.0 μMMicrosource (Natural product library)50247450.0111111
0.0992.55E-14SGTC_1753st043925 64.0 μMTimTec (Natural product derivative library)6701810.117021
0.0921.67E-12SGTC_2419paclitaxel 166.5 μMMiscellaneous46660.0955882
0.0822.25E-10SGTC_2414st077232 92.9 μMTimTec (Natural product derivative library)7298010.0795455
0.0823.37E-10SGTC_15120259-0544 12.2 μMChemDiv (Drug-like library)184760.11236
0.0756.75E-9SGTC_1911st060208 74.3 μMTimTec (Natural product derivative library)6887830.103448
0.0722.45E-8SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.0833333
0.0713.97E-8SGTC_31569099931 49.5 μMChembridge (Drug-like library)181110530.131868
0.0706.27E-8SGTC_30159080845 49.5 μMChembridge (Drug-like library)52969730.113402
0.0681.82E-7SGTC_15024534-4282 27.7 μMChemDiv (Drug-like library)32789490.11828
0.0673.05E-7SGTC_1949st076024 69.1 μMTimTec (Natural product derivative library)29219300.114583
0.0612.34E-6SGTC_1852st056254 31.4 μMTimTec (Natural product derivative library)4425830.11236
0.0604.26E-6SGTC_1563conessine 56.1 μMTimTec (Pure natural product library)4410820.0480769

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1039k821-004394 μM0.52702715992768ChemDiv (Drug-like library)347.819263.31814Golgi
SGTC_6293448-646676.7 μM0.394366573744ChemDiv (Drug-like library)254.3072.75413
SGTC_22167189485200 μM0.385714731273Chembridge (Fragment library)236.290180.95604
SGTC_3118912425049.47 μM0.26251504786Chembridge (Drug-like library)317.16944.12912
SGTC_3269913782349.47 μM0.24418617682936Chembridge (Drug-like library)309.38552.9041460S ribosome export
SGTC_3215912994249.47 μM0.23863617682944Chembridge (Drug-like library)323.412083.36114
SGTC_2159565861173.75 μM0.2375793844Chembridge (Fragment library)289.35281.9234
SGTC_2968909066333.37 μM0.23529425236811Chembridge (Drug-like library)325.224123.43413
SGTC_2913796655636.57 μM0.2333332976516Chembridge (Drug-like library)372.43813.3982560S ribosome export
SGTC_3337914434233.55 μM0.23255817682949Chembridge (Drug-like library)313.3493832.62415