3966-0071

methyl benzo[b][1]benzazepine-11-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1415
Screen concentration 78.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 2893185
SMILES COC(=O)N1C2=CC=CC=C2C=CC3=CC=CC=C31
Standardized SMILES COC(=O)N1c2ccccc2C=Cc3ccccc13
Molecular weight 251.2799
ALogP 3.53
H-bond donor count 0
H-bond acceptor count 2
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.56
% growth inhibition (Hom. pool) 5.1


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2893185
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCL1(YPR025C)|FD-Score:-3.8|P-value:7.38E-5|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CEP3(YMR168C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.17||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CIA2(YHR122W)|FD-Score:-3.57|P-value:1.76E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:ERG12(YMR208W)|FD-Score:4.43|P-value:4.82E-6|Clearance:0.46||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GRS1(YBR121C)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HEM4(YOR278W)|FD-Score:4.37|P-value:6.11E-6|Clearance:0.46||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MRD1(YPR112C)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.02||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NAT2(YGR147C)|FD-Score:3.16|P-value:7.80E-4|Clearance:0.05||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOP19(YGR251W)|FD-Score:-3.21|P-value:6.58E-4|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:PFS2(YNL317W)|FD-Score:3.73|P-value:9.57E-5|Clearance:0.11||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PMI40(YER003C)|FD-Score:4.65|P-value:1.67E-6|Clearance:0.46||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:4.36|P-value:6.54E-6|Clearance:0.46||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPL18A(YOL120C)|FD-Score:5.49|P-value:1.96E-8|Clearance:0.46||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRN7(YJL025W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.04||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP46(YGR095C)|FD-Score:4.49|P-value:3.58E-6|Clearance:0.46||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRS1(YOR294W)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.46||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SEC6(YIL068C)|FD-Score:-3.46|P-value:2.75E-4|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SED5(YLR026C)|FD-Score:-3.35|P-value:4.11E-4|Clearance:0||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:SPC97(YHR172W)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.46||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPT15(YER148W)|FD-Score:4.91|P-value:4.55E-7|Clearance:0.46||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUA5(YGL169W)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.04||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI1(YNL244C)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFC6(YDR362C)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.46||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIM23(YNR017W)|FD-Score:-4.05|P-value:2.59E-5|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:USO1(YDL058W)|FD-Score:3.4|P-value:3.31E-4|Clearance:0.15||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:UTR5(YEL035C_p)|FD-Score:3.21|P-value:6.61E-4|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:VHT1(YGR065C)|FD-Score:3.23|P-value:6.27E-4|Clearance:0.02||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YCL041C(YCL041C_d)|FD-Score:-3.95|P-value:3.94E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:ACT1(YFL039C)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCL1(YPR025C)|FD-Score:-3.8|P-value:7.38E-5|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CEP3(YMR168C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.17||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CIA2(YHR122W)|FD-Score:-3.57|P-value:1.76E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:ERG12(YMR208W)|FD-Score:4.43|P-value:4.82E-6|Clearance:0.46||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GRS1(YBR121C)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HEM4(YOR278W)|FD-Score:4.37|P-value:6.11E-6|Clearance:0.46||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MRD1(YPR112C)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.02||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NAT2(YGR147C)|FD-Score:3.16|P-value:7.80E-4|Clearance:0.05||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOP19(YGR251W)|FD-Score:-3.21|P-value:6.58E-4|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:PFS2(YNL317W)|FD-Score:3.73|P-value:9.57E-5|Clearance:0.11||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PMI40(YER003C)|FD-Score:4.65|P-value:1.67E-6|Clearance:0.46||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:4.36|P-value:6.54E-6|Clearance:0.46||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPL18A(YOL120C)|FD-Score:5.49|P-value:1.96E-8|Clearance:0.46||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRN7(YJL025W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.04||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP46(YGR095C)|FD-Score:4.49|P-value:3.58E-6|Clearance:0.46||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRS1(YOR294W)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.46||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SEC6(YIL068C)|FD-Score:-3.46|P-value:2.75E-4|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SED5(YLR026C)|FD-Score:-3.35|P-value:4.11E-4|Clearance:0||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:SPC97(YHR172W)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.46||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPT15(YER148W)|FD-Score:4.91|P-value:4.55E-7|Clearance:0.46||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUA5(YGL169W)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.04||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI1(YNL244C)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFC6(YDR362C)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.46||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIM23(YNR017W)|FD-Score:-4.05|P-value:2.59E-5|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:USO1(YDL058W)|FD-Score:3.4|P-value:3.31E-4|Clearance:0.15||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:UTR5(YEL035C_p)|FD-Score:3.21|P-value:6.61E-4|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:VHT1(YGR065C)|FD-Score:3.23|P-value:6.27E-4|Clearance:0.02||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YCL041C(YCL041C_d)|FD-Score:-3.95|P-value:3.94E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2893185
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADH7(YCR105W)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:AIM33(YML087C)|FD-Score:-3.8|P-value:7.12E-5||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARA2(YMR041C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:BMH2(YDR099W)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CMC1(YKL137W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COG7(YGL005C)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.79|P-value:8.31E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CRH1(YGR189C)|FD-Score:-4.79|P-value:8.41E-7||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTK1(YKL139W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL1(YIR027C)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DTR1(YBR180W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:DUS4(YLR405W)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EMP24(YGL200C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP3(YDL018C)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR7(YFR008W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FCY22(YER060W-A)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GAP1(YKR039W)|FD-Score:-4.68|P-value:1.45E-6||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GLG1(YKR058W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GNP1(YDR508C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HOM2(YDR158W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HST1(YOL068C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IMP1(YMR150C)|FD-Score:-4.38|P-value:6.03E-6||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:LDB19(YOR322C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MRPL32(YCR003W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MUP3(YHL036W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NOP16(YER002W)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NPP2(YEL016C)|FD-Score:-4.01|P-value:2.97E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NRM1(YNR009W)|FD-Score:-3.95|P-value:3.85E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NUM1(YDR150W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PEP7(YDR323C)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX5(YDR244W)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PPG1(YNR032W)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:RAV1(YJR033C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCF1(YML030W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RPL12B(YDR418W)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL18B(YNL301C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSF2(YJR127C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SKG3(YLR187W_p)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SSA1(YAL005C)|FD-Score:-4.48|P-value:3.78E-6||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:TAT1(YBR069C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRS85(YDR108W)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VPS45(YGL095C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL028C(YBL028C)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBL086C(YBL086C_p)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YBR090C(YBR090C_p)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR134W(YBR134W_d)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR094W(YDR094W_d)|FD-Score:-4.36|P-value:6.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YER068C-A(YER068C-A_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR022C(YGR022C_d)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Gene:YIR043C(YIR043C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKL202W(YKL202W_d)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR034C(YMR034C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR135W-A(YMR135W-A_d)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YNL042W-B(YNL042W-B_p)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function Gene:YNL146W(YNL146W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR283W(YOR283W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR127W(YPR127W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AAD4(YDL243C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADH7(YCR105W)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:AIM33(YML087C)|FD-Score:-3.8|P-value:7.12E-5||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARA2(YMR041C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:BMH2(YDR099W)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CMC1(YKL137W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COG7(YGL005C)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.79|P-value:8.31E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CRH1(YGR189C)|FD-Score:-4.79|P-value:8.41E-7||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTK1(YKL139W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL1(YIR027C)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DTR1(YBR180W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:DUS4(YLR405W)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EMP24(YGL200C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP3(YDL018C)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR7(YFR008W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FCY22(YER060W-A)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GAP1(YKR039W)|FD-Score:-4.68|P-value:1.45E-6||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GLG1(YKR058W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GNP1(YDR508C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HOM2(YDR158W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HST1(YOL068C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IMP1(YMR150C)|FD-Score:-4.38|P-value:6.03E-6||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:LDB19(YOR322C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MRPL32(YCR003W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MUP3(YHL036W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NOP16(YER002W)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NPP2(YEL016C)|FD-Score:-4.01|P-value:2.97E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NRM1(YNR009W)|FD-Score:-3.95|P-value:3.85E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NUM1(YDR150W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PEP7(YDR323C)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX5(YDR244W)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PPG1(YNR032W)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:RAV1(YJR033C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCF1(YML030W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RPL12B(YDR418W)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL18B(YNL301C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSF2(YJR127C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SKG3(YLR187W_p)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SSA1(YAL005C)|FD-Score:-4.48|P-value:3.78E-6||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:TAT1(YBR069C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRS85(YDR108W)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VPS45(YGL095C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL028C(YBL028C)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBL086C(YBL086C_p)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YBR090C(YBR090C_p)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR134W(YBR134W_d)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR094W(YDR094W_d)|FD-Score:-4.36|P-value:6.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YER068C-A(YER068C-A_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR022C(YGR022C_d)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Gene:YIR043C(YIR043C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKL202W(YKL202W_d)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR034C(YMR034C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR135W-A(YMR135W-A_d)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YNL042W-B(YNL042W-B_p)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function Gene:YNL146W(YNL146W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR283W(YOR283W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR127W(YPR127W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL120C5.491.96E-80.46RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YOR294W5.201.02E-70.46RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YER148W4.914.55E-70.46SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YDR362C4.721.19E-60.46TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YER003C4.651.67E-60.46PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YGR095C4.493.58E-60.46RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YMR208W4.434.82E-60.46ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YOR278W4.376.11E-60.46HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YGR156W4.366.54E-60.46PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YHR172W4.191.39E-50.46SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YNL317W3.739.57E-50.11PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YMR168C3.621.49E-40.17CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YJL025W3.452.81E-40.04RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YDL058W3.403.31E-40.15USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YFL039C3.255.69E-40.03ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR458C5.393.47E-8HEH2Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
YDR418W4.934.03E-7RPL12BRibosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
YML071C4.798.31E-7COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL148C4.631.83E-6ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YBR134W_d4.483.67E-6YBR134W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR058C4.454.38E-6TGL2Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
YJR033C4.201.31E-5RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YKL139W4.141.77E-5CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YGL200C4.131.83E-5EMP24Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
YDR129C4.062.48E-5SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YGL005C3.816.90E-5COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR323C3.807.26E-5PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YIR027C3.787.94E-5DAL1Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
YMR166C_p3.631.42E-4YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YDR158W3.601.57E-4HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis

GO enrichment analysis for SGTC_1415
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2666.98E-96SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0735294DNA intercalators
0.2525.15E-86SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.169492DNA intercalators
0.2251.45E-68SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.153846DNA intercalators
0.1998.23E-54SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0612245DNA intercalators
0.1971.81E-52SGTC_512prima-1 270.0 μMICCB bioactive library3229680.0638298DNA intercalators
0.1917.58E-50SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0727273DNA intercalators
0.1817.05E-45SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.166667DNA intercalators
0.1641.03E-36SGTC_470aa-861 153.0 μMICCB bioactive library19670.0566038sphingolipid biosynthesis & PDR1
0.1632.42E-36SGTC_32839109332 35.8 μMChembridge (Drug-like library)170272810.15625DNA intercalators
0.1627.34E-36SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.148148DNA intercalators
0.1547.95E-33SGTC_511blebbistatin 171.0 μMICCB bioactive library34769860.131148DNA intercalators
0.1455.47E-29SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.166667
0.1449.31E-29SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.131579DNA intercalators
0.1344.53E-25SGTC_264promethazine 12.5 mMMiscellaneous60140.195652DNA intercalators
0.1314.06E-24SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0833333Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12460422-0085105 μM0.4264295ChemDiv (Drug-like library)161.157381.26512
SGTC_7450469-0705296 μM0.3333333486736ChemDiv (Drug-like library)238.153741.86706mitochondrial processes
SGTC_10234482-388816 μM0.31253303168ChemDiv (Drug-like library)267.324062.58205TSC3-RPN4
SGTC_20335192550145 μM0.285714182073Chembridge (Fragment library)189.210541.14602
SGTC_10204335-096193.3 μM0.28669075ChemDiv (Drug-like library)267.322382.91302
SGTC_1915st06710530.6 μM0.27777869751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_23117694451200 μM0.2765962163142Chembridge (Fragment library)205.2531.31602
SGTC_1527coumarin137 μM0.27027323TimTec (Pure natural product library)146.142741.89902
SGTC_3232913287449.47 μM0.2666673784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_2775cyproheptadine69.59 μM0.2619052913Miscellaneous287.398144.36201