3966-0321

3-benzyl-2H-phthalazine-1,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1416
Screen concentration 25.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 582530
SMILES C1=CC=C(C=C1)CN2C(=O)C3=CC=CC=C3C(=O)N2
Standardized SMILES O=C1NN(Cc2ccccc2)C(=O)c3ccccc13
Molecular weight 252.268
ALogP 1.83
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.75
% growth inhibition (Hom. pool) 2.68


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 582530
Download HIP data (tab-delimited text)  (excel)
Gene:HRP1(YOL123W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.09||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:POP4(YBR257W)|FD-Score:3.74|P-value:9.23E-5|Clearance:0.1||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP5(YBR237W)|FD-Score:-3.76|P-value:8.54E-5|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPA43(YOR340C)|FD-Score:3.9|P-value:4.78E-5|Clearance:0.16||SGD DESC:RNA polymerase I subunit A43 Gene:RPB8(YOR224C)|FD-Score:3.29|P-value:5.09E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO31(YOR116C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.03||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPT1(YKL145W)|FD-Score:4.54|P-value:2.78E-6|Clearance:0.46||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:25.5|P-value:3.16E-144|Clearance:19.96||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:4.09|P-value:2.18E-5|Clearance:0.19||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMP3(YOR149C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.21||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SMX2(YFL017W-A)|FD-Score:3.24|P-value:5.91E-4|Clearance:0.23||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPT15(YER148W)|FD-Score:5.59|P-value:1.15E-8|Clearance:1.05||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:YLL037W(YLL037W_d)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:HRP1(YOL123W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.09||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:POP4(YBR257W)|FD-Score:3.74|P-value:9.23E-5|Clearance:0.1||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP5(YBR237W)|FD-Score:-3.76|P-value:8.54E-5|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPA43(YOR340C)|FD-Score:3.9|P-value:4.78E-5|Clearance:0.16||SGD DESC:RNA polymerase I subunit A43 Gene:RPB8(YOR224C)|FD-Score:3.29|P-value:5.09E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO31(YOR116C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.03||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPT1(YKL145W)|FD-Score:4.54|P-value:2.78E-6|Clearance:0.46||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:25.5|P-value:3.16E-144|Clearance:19.96||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:4.09|P-value:2.18E-5|Clearance:0.19||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMP3(YOR149C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.21||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SMX2(YFL017W-A)|FD-Score:3.24|P-value:5.91E-4|Clearance:0.23||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPT15(YER148W)|FD-Score:5.59|P-value:1.15E-8|Clearance:1.05||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:YLL037W(YLL037W_d)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 582530
Download HOP data (tab-delimited text)  (excel)
Gene:API2(YDR525W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APT2(YDR441C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BAG7(YOR134W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:CCS1(YMR038C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DDI3(YNL335W_p)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ENT1(YDL161W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FKS1(YLR342W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GOS1(YHL031C)|FD-Score:5.94|P-value:1.44E-9||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GRE1(YPL223C)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:HHF1(YBR009C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HTA2(YBL003C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IES6(YEL044W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRS4(YKR019C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISC10(YER180C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MDH2(YOL126C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MND2(YIR025W)|FD-Score:-4.51|P-value:3.17E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNT4(YNR059W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRP4(YHL004W)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVP1(YMR004W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:MZM1(YDR493W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NEW1(YPL226W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NGR1(YBR212W)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NUP60(YAR002W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAF1(YBR279W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PET111(YMR257C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PHO4(YFR034C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:3.82|P-value:6.68E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHR1(YOR386W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIM1(YBL022C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMT7(YDR307W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RCF2(YNR018W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RIC1(YLR039C)|FD-Score:4.86|P-value:5.91E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL12A(YEL054C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:6.72|P-value:9.10E-12||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:9.06|P-value:6.79E-20||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRI2(YOL117W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SCP1(YOR367W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SFT2(YBL102W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SNC1(YAL030W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SPF1(YEL031W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO20(YMR017W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SST2(YLR452C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:SWA2(YDR320C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TOM70(YNL121C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPK2(YPL203W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRM7(YBR061C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP2(YER090W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBX2(YML013W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VAC7(YNL054W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS61(YDR136C_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCR100C(YCR100C_p)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Putative protein of unknown function Gene:YER091C-A(YER091C-A_d)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL168W(YIL168W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL211C(YJL211C_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR366W(YLR366W_d)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR099C(YMR099C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL235C(YNL235C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOR121C(YOR121C_d)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL102C(YPL102C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:API2(YDR525W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APT2(YDR441C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BAG7(YOR134W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:CCS1(YMR038C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DDI3(YNL335W_p)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ENT1(YDL161W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FKS1(YLR342W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GOS1(YHL031C)|FD-Score:5.94|P-value:1.44E-9||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GRE1(YPL223C)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:HHF1(YBR009C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HTA2(YBL003C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IES6(YEL044W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRS4(YKR019C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISC10(YER180C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MDH2(YOL126C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MND2(YIR025W)|FD-Score:-4.51|P-value:3.17E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNT4(YNR059W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRP4(YHL004W)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVP1(YMR004W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:MZM1(YDR493W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NEW1(YPL226W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NGR1(YBR212W)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NUP60(YAR002W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAF1(YBR279W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PET111(YMR257C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PHO4(YFR034C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:3.82|P-value:6.68E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHR1(YOR386W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIM1(YBL022C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMT7(YDR307W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RCF2(YNR018W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RIC1(YLR039C)|FD-Score:4.86|P-value:5.91E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL12A(YEL054C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:6.72|P-value:9.10E-12||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:9.06|P-value:6.79E-20||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRI2(YOL117W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SCP1(YOR367W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SFT2(YBL102W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SNC1(YAL030W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SPF1(YEL031W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO20(YMR017W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SST2(YLR452C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:SWA2(YDR320C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TOM70(YNL121C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPK2(YPL203W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRM7(YBR061C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP2(YER090W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBX2(YML013W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VAC7(YNL054W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS61(YDR136C_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCR100C(YCR100C_p)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Putative protein of unknown function Gene:YER091C-A(YER091C-A_d)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL168W(YIL168W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL211C(YJL211C_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR366W(YLR366W_d)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR099C(YMR099C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL235C(YNL235C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOR121C(YOR121C_d)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL102C(YPL102C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W25.503.16E-14420.00SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YER148W5.591.15E-81.05SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YKL145W4.542.78E-60.46RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDL195W4.092.18E-50.19SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YOR340C3.904.78E-50.16RPA43RNA polymerase I subunit A43
YBR257W3.749.23E-50.10POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YOL123W3.641.37E-40.09HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YOR116C3.551.96E-40.04RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YLL037W_d3.512.24E-40.02YLL037W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
YOR149C3.492.39E-40.21SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YOR224C3.295.09E-40.04RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YFL017W-A3.245.91E-40.23SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
YML092C3.020.001280.05PRE8Alpha 2 subunit of the 20S proteasome
YER009W2.970.001510.07NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YPR183W2.900.001870.00DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL020C9.066.79E-20RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YHR010W6.729.10E-12RPL27ARibosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
YHL031C5.941.44E-9GOS1v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28
YLR039C4.865.91E-7RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
YNR018W4.691.34E-6RCF2Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
YNL054W4.261.04E-5VAC7Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock
YER090W4.241.12E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YEL044W4.241.12E-5IES6Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
YIL012W_d4.241.12E-5YIL012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR071C4.191.42E-5TVP18Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
YPL226W4.171.50E-5NEW1ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
YPL102C_d4.092.14E-5YPL102C_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4
YNL335W_p4.072.35E-5DDI3_pProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
YMR017W3.973.52E-5SPO20Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
YDR493W3.973.64E-5MZM1Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog

GO enrichment analysis for SGTC_1416
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4630SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0877193
0.4353.46E-271SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.173077
0.4303.71E-264SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.152542
0.3863.51E-209SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.116667
0.3797.38E-201SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.0847458
0.3444.54E-164SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.2
0.3427.10E-162SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.12963
0.2818.99E-108SGTC_9933909-8757 62.7 μMChemDiv (Drug-like library)42883240.0833333
0.2661.88E-96SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.127273
0.2441.18E-80SGTC_31979113331 49.5 μMChembridge (Drug-like library)166104590.212121
0.2384.81E-77SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.125
0.2278.47E-70SGTC_1168k815-0004 80.5 μMChemDiv (Drug-like library)6424330.0909091DNA damage response
0.2261.82E-69SGTC_8780828-0289 13.7 μMChemDiv (Drug-like library)7453500.133333
0.2145.73E-62SGTC_10044084-0029 199.0 μMChemDiv (Drug-like library)7168940.116667
0.2061.89E-57SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.0655738ERAD & cell cycle

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7313966-0296387 μM0.42660956ChemDiv (Drug-like library)318.367582.44314
SGTC_14173966-032790.4 μM0.42651118ChemDiv (Drug-like library)316.35171.32314
SGTC_13161272-005065.9 μM0.3953497340392ChemDiv (Drug-like library)251.283222.02213
SGTC_544k015-002052.4 μM0.3409093008304ChemDiv (Drug-like library)316.14943.20802
SGTC_1747st03749666.6 μM0.333333723305TimTec (Natural product derivative library)300.312540.64403
SGTC_2007401153971.82 μM0.33333345595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_23427990637118.86 μM0.3333332033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport
SGTC_1456k015-001213 μM0.3191492989616ChemDiv (Drug-like library)363.149873.03802
SGTC_24015128683200 μM0.304348708373Miscellaneous344.363343.86214
SGTC_1139k015-002446.9 μM0.32766652ChemDiv (Drug-like library)305.251313.40205PDR1