3966-3099

6-(4-bromophenyl)-2-sulfanylidene-1,3-thiazin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1418
Screen concentration 2.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 740335
SMILES C1=CC(=CC=C1C2=CC(=O)NC(=S)S2)Br
Standardized SMILES Brc1ccc(cc1)C2=CC(=O)NC(=S)S2
Molecular weight 300.1947
ALogP 3.59
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.29
% growth inhibition (Hom. pool) 4.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 740335
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:-3.73|P-value:9.65E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:3.76|P-value:8.65E-5|Clearance:0.39||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.15|P-value:8.07E-4|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI16(YHR188C)|FD-Score:3.97|P-value:3.56E-5|Clearance:0.22||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:JAC1(YGL018C)|FD-Score:3.11|P-value:9.22E-4|Clearance:0.03||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:MTR10(YOR160W)|FD-Score:-3.14|P-value:8.34E-4|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:RER2(YBR002C)|FD-Score:5.12|P-value:1.53E-7|Clearance:1.15||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC2(YJR068W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.08||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPN3(YER021W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:-3.42|P-value:3.10E-4|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RRP14(YKL082C)|FD-Score:3.15|P-value:8.05E-4|Clearance:0||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC58(YLR033W)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RTT105(YER104W)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.05||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SDO1(YLR022C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.05||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC13(YLR208W)|FD-Score:6.67|P-value:1.28E-11|Clearance:1.55||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YRB1(YDR002W)|FD-Score:-3.26|P-value:5.50E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:CCT7(YJL111W)|FD-Score:-3.73|P-value:9.65E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:3.76|P-value:8.65E-5|Clearance:0.39||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.15|P-value:8.07E-4|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI16(YHR188C)|FD-Score:3.97|P-value:3.56E-5|Clearance:0.22||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:JAC1(YGL018C)|FD-Score:3.11|P-value:9.22E-4|Clearance:0.03||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:MTR10(YOR160W)|FD-Score:-3.14|P-value:8.34E-4|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:RER2(YBR002C)|FD-Score:5.12|P-value:1.53E-7|Clearance:1.15||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC2(YJR068W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.08||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPN3(YER021W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:-3.42|P-value:3.10E-4|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RRP14(YKL082C)|FD-Score:3.15|P-value:8.05E-4|Clearance:0||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC58(YLR033W)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RTT105(YER104W)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.05||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SDO1(YLR022C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.05||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC13(YLR208W)|FD-Score:6.67|P-value:1.28E-11|Clearance:1.55||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YRB1(YDR002W)|FD-Score:-3.26|P-value:5.50E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 740335
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADH6(YMR318C)|FD-Score:4|P-value:3.18E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AHC2(YCR082W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM26(YKL037W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ARG1(YOL058W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO1(YDR127W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP6(YLR085C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BCH1(YMR237W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BEM2(YER155C)|FD-Score:4.76|P-value:9.75E-7||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:CCM1(YGR150C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CKB1(YGL019W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:DPH1(YIL103W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ERG5(YMR015C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ERV15(YBR210W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FAR7(YFR008W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:GCN3(YKR026C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:10.8|P-value:1.53E-27||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:IDH2(YOR136W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IKI1(YHR187W)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:KTI11(YBL071W-A)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LEE1(YPL054W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LSB6(YJL100W)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MIP1(YOR330C)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MRPL23(YOR150W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NIT2(YJL126W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NPP2(YEL016C)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NSR1(YGR159C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:PAU23(YLR037C)|FD-Score:4.85|P-value:6.16E-7||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PHM8(YER037W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PSD2(YGR170W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:RKM3(YBR030W)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RPL12B(YDR418W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SER1(YOR184W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SLT2(YHR030C)|FD-Score:7.77|P-value:3.99E-15||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNA3(YJL151C)|FD-Score:5.36|P-value:4.14E-8||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SPE2(YOL052C)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SRY1(YKL218C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:TMA46(YOR091W)|FD-Score:-4.27|P-value:9.99E-6||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TOR1(YJR066W)|FD-Score:5.43|P-value:2.76E-8||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:9.24|P-value:1.18E-20||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.76|P-value:6.67E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.1|P-value:5.45E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:XYL2(YLR070C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YAL037W(YAL037W_p)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAR029W(YAR029W_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBR174C(YBR174C_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR102C(YCR102C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR008C(YDR008C_d)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function Gene:YGR201C(YGR201C_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function Gene:YGR228W(YGR228W_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YGR269W(YGR269W_d)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIA6(YIL006W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YLR124W(YLR124W_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL217W(YNL217W_p)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOR097C(YOR097C_p)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL182C(YPL182C_d)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YSP2(YDR326C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADH6(YMR318C)|FD-Score:4|P-value:3.18E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AHC2(YCR082W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM26(YKL037W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ARG1(YOL058W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO1(YDR127W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP6(YLR085C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BCH1(YMR237W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BEM2(YER155C)|FD-Score:4.76|P-value:9.75E-7||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:CCM1(YGR150C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CKB1(YGL019W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:DPH1(YIL103W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ERG5(YMR015C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ERV15(YBR210W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FAR7(YFR008W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:GCN3(YKR026C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:10.8|P-value:1.53E-27||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:IDH2(YOR136W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IKI1(YHR187W)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:KTI11(YBL071W-A)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LEE1(YPL054W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LSB6(YJL100W)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MIP1(YOR330C)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MRPL23(YOR150W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NIT2(YJL126W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NPP2(YEL016C)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NSR1(YGR159C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:PAU23(YLR037C)|FD-Score:4.85|P-value:6.16E-7||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PHM8(YER037W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PSD2(YGR170W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:RKM3(YBR030W)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RPL12B(YDR418W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SER1(YOR184W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SLT2(YHR030C)|FD-Score:7.77|P-value:3.99E-15||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNA3(YJL151C)|FD-Score:5.36|P-value:4.14E-8||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SPE2(YOL052C)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SRY1(YKL218C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:TMA46(YOR091W)|FD-Score:-4.27|P-value:9.99E-6||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TOR1(YJR066W)|FD-Score:5.43|P-value:2.76E-8||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:9.24|P-value:1.18E-20||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.76|P-value:6.67E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.1|P-value:5.45E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:XYL2(YLR070C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YAL037W(YAL037W_p)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAR029W(YAR029W_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBR174C(YBR174C_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR102C(YCR102C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR008C(YDR008C_d)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function Gene:YGR201C(YGR201C_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function Gene:YGR228W(YGR228W_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YGR269W(YGR269W_d)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIA6(YIL006W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YLR124W(YLR124W_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL217W(YNL217W_p)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOR097C(YOR097C_p)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL182C(YPL182C_d)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YSP2(YDR326C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W6.671.28E-111.55SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR002C5.121.53E-71.15RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YHR188C3.973.56E-50.22GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YGL116W3.768.65E-50.39CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YJR068W3.363.84E-40.08RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YLR022C3.285.12E-40.05SDO1Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
YER104W3.236.14E-40.05RTT105Protein with a role in regulation of Ty1 transposition
YLR033W3.187.37E-40.03RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YKL082C3.158.05E-48.24E-4RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YER133W3.158.07E-40.04GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YGL018C3.119.22E-40.03JAC1Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
YBR252W3.080.001030.01DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YGR175C3.080.001050.02ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YOR159C3.060.001120.05SME1Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
YJL086C_d3.000.001340.01YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL009C10.801.53E-27GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR007W9.241.18E-20TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YHR030C7.773.99E-15SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YKL211C6.766.67E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W6.105.45E-10TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YJR066W5.432.76E-8TOR1PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
YJL151C5.364.14E-8SNA3Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles
YGL026C5.141.38E-7TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YLR037C4.856.16E-7PAU23Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
YER155C4.769.75E-7BEM2Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence
YKL037W4.731.12E-6AIM26Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT
YIL103W4.513.26E-6DPH1Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
YOR330C4.454.31E-6MIP1Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
YNL217W_p4.102.02E-5YNL217W_pPutative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
YMR318C4.003.18E-5ADH6NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1418
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4227.91E-254SGTC_18825615643 20.0 μMMiscellaneous22530750.128571TRP & mitochondrial translation
0.4031.59E-229SGTC_24885268135 45.7 μMMiscellaneous12703560.270833
0.3836.97E-206SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0634921
0.3827.29E-205SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0634921
0.3818.95E-203SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.295455TRP & mitochondrial translation
0.3693.02E-190SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0689655
0.3681.34E-188SGTC_24785763493 32.3 μMMiscellaneous13655760.245283
0.3645.89E-185SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.0535714
0.3593.48E-179SGTC_18815652484 16.0 μMMiscellaneous22551290.147059TRP & mitochondrial translation
0.3581.97E-178SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0123457
0.3508.04E-170SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0533333
0.3432.33E-162SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.02
0.3384.75E-158SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.163636
0.3342.27E-153SGTC_14234048-0112 5.6 μMChemDiv (Drug-like library)X14230.0512821
0.3303.28E-150SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.046875

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_18835-benzylidenerhodanine20 μM0.2954551273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.2954551273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_13241418-002634.4 μM0.2857141372513ChemDiv (Drug-like library)394.06073.79712
SGTC_12740865-0114173 μM0.27907870624ChemDiv (Drug-like library)255.068081.3712
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.2708331241132ChemDiv (Drug-like library)227.32642.79414
SGTC_2488526813545.7 μM0.2708331270356Miscellaneous247.335963.30913
SGTC_20545233173129 μM0.2619056889Chembridge (Fragment library)226.026861.41812mitochondrial response to ROS
SGTC_1862st0575133.81 μM0.265393163TimTec (Natural product derivative library)317.134163.88413
SGTC_21435623677140.46 μM0.2549025375313Chembridge (Fragment library)280.2980031.96125
SGTC_2472537765249.4 μM0.2549021610903Miscellaneous261.362543.75513TRP & mitochondrial translation
SGTC_1060092-000311.58 μM0.251072148ChemDiv (Drug-like library)355.182143.67503copper-dependent oxidative stress