4049-0260

5-chloro-N-[4-(4-chlorophenoxy)phenyl]-1,3-dimethylpyrazole-4-sulfonamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1431
Screen concentration 9.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 3854841
SMILES CC1=NN(C(=C1S(=O)(=O)NC2=CC=C(C=C2)OC3=CC=C(C=C3)Cl)Cl)C
Standardized SMILES Cc1nn(C)c(Cl)c1S(=O)(=O)Nc2ccc(Oc3ccc(Cl)cc3)cc2
Molecular weight 412.2903
ALogP 3.93
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.76
% growth inhibition (Hom. pool) 1.35


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3854841
Download HIP data (tab-delimited text)  (excel)
Gene:CHS2(YBR038W)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.03||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:GCD10(YNL062C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.07||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:MCM6(YGL201C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.04||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:NSL1(YPL233W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:SPT16(YGL207W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.29||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TEL2(YGR099W)|FD-Score:4.82|P-value:7.14E-7|Clearance:1.06||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:WRS1(YOL097C)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.04||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YNL114C(YNL114C_d)|FD-Score:3.76|P-value:8.39E-5|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:CHS2(YBR038W)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.03||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:GCD10(YNL062C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.07||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:MCM6(YGL201C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.04||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:NSL1(YPL233W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:SPT16(YGL207W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.29||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TEL2(YGR099W)|FD-Score:4.82|P-value:7.14E-7|Clearance:1.06||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:WRS1(YOL097C)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.04||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YNL114C(YNL114C_d)|FD-Score:3.76|P-value:8.39E-5|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3854841
Download HOP data (tab-delimited text)  (excel)
Gene:AIM33(YML087C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:AIM44(YPL158C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ATC1(YDR184C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG2(YNL242W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATG8(YBL078C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:COQ5(YML110C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CPR6(YLR216C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CWH41(YGL027C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL7(YIR031C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DPB4(YDR121W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:EMC4(YGL231C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:GNP1(YDR508C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:IZH1(YDR492W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:KEX1(YGL203C)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KIN82(YCR091W)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LYS4(YDR234W)|FD-Score:6.3|P-value:1.50E-10||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MAF1(YDR005C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MLC2(YPR188C)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MNN2(YBR015C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:PLB1(YMR008C)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:RGS2(YOR107W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:ROM1(YGR070W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPS25A(YGR027C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SKT5(YBL061C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:URA5(YML106W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR222W(YDR222W_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR445C(YDR445C_d)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL242C(YGL242C_p)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YIL067C(YIL067C_p)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJU3(YKL094W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YMR057C(YMR057C_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YOL150C(YOL150C_d)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR225W(YOR225W_d)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AIM33(YML087C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:AIM44(YPL158C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ATC1(YDR184C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG2(YNL242W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATG8(YBL078C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:COQ5(YML110C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CPR6(YLR216C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CWH41(YGL027C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL7(YIR031C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DPB4(YDR121W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:EMC4(YGL231C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:GNP1(YDR508C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:IZH1(YDR492W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:KEX1(YGL203C)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KIN82(YCR091W)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LYS4(YDR234W)|FD-Score:6.3|P-value:1.50E-10||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MAF1(YDR005C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MLC2(YPR188C)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MNN2(YBR015C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:PLB1(YMR008C)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:RGS2(YOR107W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:ROM1(YGR070W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPS25A(YGR027C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SKT5(YBL061C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:URA5(YML106W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR222W(YDR222W_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR445C(YDR445C_d)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL242C(YGL242C_p)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YIL067C(YIL067C_p)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJU3(YKL094W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YMR057C(YMR057C_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YOL150C(YOL150C_d)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR225W(YOR225W_d)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR099W4.827.14E-71.06TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YNL114C_d3.768.39E-50.23YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YGL207W3.532.08E-40.29SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YNL062C3.246.07E-40.07GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YBR038W3.177.70E-40.03CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YOL097C3.148.58E-40.04WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YGL201C3.109.80E-40.04MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YEL002C3.050.001140.05WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YGR030C3.000.001340.12POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR048W2.880.001970.01UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YDR091C2.870.002030.03RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YNL002C2.840.002240.05RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YHR197W2.790.002600.03RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YPR180W2.770.002850.07AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YBR142W2.700.003490.06MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR234W6.301.50E-10LYS4Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
YGL203C5.171.20E-7KEX1Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
YGL242C_p3.963.73E-5YGL242C_pPutative protein of unknown function; deletion mutant is viable
YOR225W_d3.924.39E-5YOR225W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR188C3.816.90E-5MLC2Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
YFL042C_p3.797.51E-5YFL042C_pPutative protein of unknown function; YFL042C is not an essential gene
YOR107W3.797.55E-5RGS2Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
YCR091W3.739.57E-5KIN82Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p
YGR070W3.611.50E-4ROM1GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
YBL061C3.561.84E-4SKT5Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
YDR121W3.452.78E-4DPB4Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
YBR182C-A_p3.442.89E-4YBR182C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YBL078C3.373.70E-4ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YML110C3.334.38E-4COQ52-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
YGL027C3.314.65E-4CWH41Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress

GO enrichment analysis for SGTC_1431
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2531.08E-86SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.140845
0.2177.38E-64SGTC_14123909-8563 178.0 μMChemDiv (Drug-like library)237675300.0967742
0.1376.05E-26SGTC_15120259-0544 12.2 μMChemDiv (Drug-like library)184760.132353
0.1102.21E-17SGTC_2419paclitaxel 166.5 μMMiscellaneous46660.0672269
0.1016.66E-15SGTC_820054-0107 293.8 μMChemDiv (Drug-like library)31046970.117647
0.0979.51E-14SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.210526
0.0806.45E-10SGTC_24275545055 42.8 μMMiscellaneous53728580.121212
0.0791.31E-9SGTC_25422-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 100.0 μMMicrosource (Natural product library)50247450.0147059
0.0741.07E-8SGTC_24045161160 200.0 μMMiscellaneous28315430.135135
0.0723.01E-8SGTC_1777st045864 34.8 μMTimTec (Natural product derivative library)46914620.05
0.0691.36E-7SGTC_24805767542 37.2 μMMiscellaneous28673580.0487805
0.0672.47E-7SGTC_6470140-0236 81.0 μMChemDiv (Drug-like library)9908370.0677966
0.0672.57E-7SGTC_508roscovitine 141.0 μMICCB bioactive library1603550.0843373
0.0663.59E-7SGTC_510ag-490 170.0 μMICCB bioactive library53287790.0657895
0.0647.34E-7SGTC_23599039622 200.0 μMChembridge (Fragment library)64635960.0769231endomembrane recycling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1994063-002441.6 μM0.431373306215ChemDiv (Drug-like library)311.783863.31414
SGTC_2920798290415.77 μM0.3389836456808Chembridge (Drug-like library)329.7743233.51915
SGTC_5414513-132194 μM0.333333747515ChemDiv (Drug-like library)305.391963.51314
SGTC_3346915825443.58 μM0.32758617639500Chembridge (Drug-like library)311.783863.31414
SGTC_2820798963071.43 μM0.303032984098Chembridge (Drug-like library)416.877983.7525ERG2
SGTC_13251431-0015227 μM0.3018871103340ChemDiv (Drug-like library)352.620824.06824Golgi
SGTC_5983937-023620.2 μM0.31379313ChemDiv (Drug-like library)354.80864.33515ERG2
SGTC_14974476-297841.3 μM0.2833331528379ChemDiv (Drug-like library)331.310193.24317
SGTC_3180910928749.47 μM0.28333318891271Chembridge (Drug-like library)311.783862.97214
SGTC_3434130-282729.8 μM0.2807021118244ChemDiv (Drug-like library)354.6118834.51614