4092-0789

1-[(2-chlorophenyl)methyl]-4-[(4-ethoxyphenyl)methyl]piperazine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1435
Screen concentration 56.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 2248285
SMILES CCOC1=CC=C(C=C1)CN2CCN(CC2)CC3=CC=CC=C3Cl
Standardized SMILES CCOc1ccc(CN2CCN(Cc3ccccc3Cl)CC2)cc1
Molecular weight 344.8783
ALogP 4.44
H-bond donor count 0
H-bond acceptor count 3
Response signature plasma membrane duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.78
% growth inhibition (Hom. pool) 3.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2248285
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.09||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC5(YMR001C)|FD-Score:-4.12|P-value:1.87E-5|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:HEM12(YDR047W)|FD-Score:5.97|P-value:1.18E-9|Clearance:1.69||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.02||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:JIP5(YPR169W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MCM1(YMR043W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.02||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MCM3(YEL032W)|FD-Score:-3.74|P-value:9.13E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NOG2(YNR053C)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.11||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:OLE1(YGL055W)|FD-Score:7.18|P-value:3.47E-13|Clearance:1.69||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:ORC5(YNL261W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0.19||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPL18A(YOL120C)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN2(YIL075C)|FD-Score:6.15|P-value:3.77E-10|Clearance:1.69||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:SGV1(YPR161C)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.06||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SSC1(YJR045C)|FD-Score:-3.26|P-value:5.54E-4|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:SUI1(YNL244C)|FD-Score:-3.89|P-value:4.97E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TEN1(YLR010C)|FD-Score:-3.17|P-value:7.70E-4|Clearance:0||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:UBA1(YKL210W)|FD-Score:3.3|P-value:4.75E-4|Clearance:0.03||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:3.36|P-value:3.89E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.54|P-value:1.47E-8|Clearance:1.69||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:BRN1(YBL097W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.09||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC5(YMR001C)|FD-Score:-4.12|P-value:1.87E-5|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:HEM12(YDR047W)|FD-Score:5.97|P-value:1.18E-9|Clearance:1.69||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HYP2(YEL034W)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.02||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:JIP5(YPR169W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MCM1(YMR043W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.02||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MCM3(YEL032W)|FD-Score:-3.74|P-value:9.13E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NOG2(YNR053C)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.11||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:OLE1(YGL055W)|FD-Score:7.18|P-value:3.47E-13|Clearance:1.69||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:ORC5(YNL261W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0.19||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPL18A(YOL120C)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN2(YIL075C)|FD-Score:6.15|P-value:3.77E-10|Clearance:1.69||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:SGV1(YPR161C)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.06||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SSC1(YJR045C)|FD-Score:-3.26|P-value:5.54E-4|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:SUI1(YNL244C)|FD-Score:-3.89|P-value:4.97E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TEN1(YLR010C)|FD-Score:-3.17|P-value:7.70E-4|Clearance:0||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:UBA1(YKL210W)|FD-Score:3.3|P-value:4.75E-4|Clearance:0.03||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:3.36|P-value:3.89E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.54|P-value:1.47E-8|Clearance:1.69||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2248285
Download HOP data (tab-delimited text)  (excel)
Gene:AIM33(YML087C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:ARC18(YLR370C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG31(YDR022C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:ATP12(YJL180C)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:CAT8(YMR280C)|FD-Score:4.38|P-value:5.85E-6||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CDC50(YCR094W)|FD-Score:7.22|P-value:2.58E-13||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB2(YOR039W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLC1(YGR167W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DRS2(YAL026C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:FYV10(YIL097W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAS1(YMR307W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN4(YEL009C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:9.04|P-value:8.17E-20||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HMG1(YML075C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HXT4(YHR092C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:KTR6(YPL053C)|FD-Score:-3.81|P-value:6.95E-5||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:KXD1(YGL079W_p)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LIP5(YOR196C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LYS12(YIL094C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:NIF3(YGL221C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPL3(YDR432W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:RCY1(YJL204C)|FD-Score:10.9|P-value:3.97E-28||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM9(YMR063W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RNP1(YLL046C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL17B(YJL177W)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS27B(YHR021C)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDH2(YLL041C)|FD-Score:6.95|P-value:1.80E-12||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SHP1(YBL058W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SHU2(YDR078C)|FD-Score:6.46|P-value:5.36E-11||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNC1(YAL030W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:TPN1(YGL186C)|FD-Score:6.27|P-value:1.83E-10||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM732(YMR259C_p)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP1(YDR007W)|FD-Score:6.94|P-value:1.94E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.62|P-value:9.47E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS1(YKR001C)|FD-Score:-4.49|P-value:3.50E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-5.66|P-value:7.54E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL059W(YBL059W_p)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR174C(YBR174C_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCL075W(YCL075W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL119C(YDL119C_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR008C(YDR008C_d)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL235W(YGL235W_p)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YIR016W(YIR016W_p)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication Gene:YLR296W(YLR296W_d)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YPL257W(YPL257W_p)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR172W(YPR172W_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AIM33(YML087C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:ARC18(YLR370C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG31(YDR022C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:ATP12(YJL180C)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:CAT8(YMR280C)|FD-Score:4.38|P-value:5.85E-6||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CDC50(YCR094W)|FD-Score:7.22|P-value:2.58E-13||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB2(YOR039W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLC1(YGR167W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DRS2(YAL026C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:FYV10(YIL097W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAS1(YMR307W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN4(YEL009C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:9.04|P-value:8.17E-20||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HMG1(YML075C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HXT4(YHR092C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:KTR6(YPL053C)|FD-Score:-3.81|P-value:6.95E-5||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:KXD1(YGL079W_p)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LIP5(YOR196C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LYS12(YIL094C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:NIF3(YGL221C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPL3(YDR432W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:RCY1(YJL204C)|FD-Score:10.9|P-value:3.97E-28||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM9(YMR063W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RNP1(YLL046C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL17B(YJL177W)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS27B(YHR021C)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDH2(YLL041C)|FD-Score:6.95|P-value:1.80E-12||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SHP1(YBL058W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SHU2(YDR078C)|FD-Score:6.46|P-value:5.36E-11||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNC1(YAL030W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:TPN1(YGL186C)|FD-Score:6.27|P-value:1.83E-10||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM732(YMR259C_p)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP1(YDR007W)|FD-Score:6.94|P-value:1.94E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.62|P-value:9.47E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS1(YKR001C)|FD-Score:-4.49|P-value:3.50E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-5.66|P-value:7.54E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL059W(YBL059W_p)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR174C(YBR174C_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCL075W(YCL075W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL119C(YDL119C_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR008C(YDR008C_d)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL235W(YGL235W_p)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YIR016W(YIR016W_p)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication Gene:YLR296W(YLR296W_d)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YPL257W(YPL257W_p)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR172W(YPR172W_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W7.183.47E-131.69OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YIL075C6.153.77E-101.69RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YDR047W5.971.18E-91.69HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YMR290W-A_d5.541.47E-81.69YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YNL261W3.855.86E-50.19ORC5Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YEL034W3.671.24E-40.02HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YPR169W3.641.36E-40.03JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YPR161C3.621.50E-40.06SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YNR053C3.561.86E-40.11NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YBL097W3.452.84E-40.09BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YDL196W_d3.363.89E-40.06YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YKL210W3.304.75E-40.03UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YOL120C3.275.37E-40.14RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YGR114C_d3.138.85E-40.01YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YMR043W3.119.28E-40.02MCM1Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C10.903.97E-28RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDL226C9.048.17E-20GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YCR094W7.222.58E-13CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YLL041C6.951.80E-12SDH2Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
YDR007W6.941.94E-12TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR078C6.465.36E-11SHU2Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
YGL186C6.271.83E-10TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YKL211C5.629.47E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YML075C5.442.60E-8HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YMR280C4.385.85E-6CAT8Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
YHR021C4.356.69E-6RPS27BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
YPL257W_p4.013.01E-5YPL257W_pPutative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
YER090W3.894.91E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YJL177W3.855.85E-5RPL17BRibosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
YJL092W3.846.15E-5SRS2DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability

GO enrichment analysis for SGTC_1435
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3702.81E-191SGTC_258haloperidol 50.8 μMMiscellaneous35590.142857fatty acid desaturase (OLE1)
0.3681.61E-188SGTC_2725haloperidol 53.2 μMMiscellaneous35590.142857plasma membrane duress
0.3621.62E-182SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.213115fatty acid desaturase (OLE1)
0.3518.89E-171SGTC_10054092-0690 4.4 μMChemDiv (Drug-like library)15293430.310345plasma membrane duress
0.3472.51E-166SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.290323plasma membrane duress
0.3437.56E-163SGTC_29107948405 26.0 μMChembridge (Drug-like library)29713280.131579plasma membrane duress
0.3433.42E-162SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.171875plasma membrane duress
0.3406.68E-160SGTC_29939054257 71.4 μMChembridge (Drug-like library)64702170.351852fatty acid desaturase (OLE1)
0.3393.09E-158SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.327273fatty acid desaturase (OLE1)
0.3335.52E-153SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.452381plasma membrane duress
0.3324.65E-152SGTC_2733amorolfine 100.0 μMMiscellaneous542600.171875plasma membrane duress
0.3327.42E-152SGTC_14754092-1048 11.7 μMChemDiv (Drug-like library)28817590.126984plasma membrane duress
0.3302.81E-150SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.20339fatty acid desaturase (OLE1)
0.3304.93E-150SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.203125fatty acid desaturase (OLE1)
0.3274.37E-147SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.140351plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7064262-005746.9 μM0.641026883298ChemDiv (Drug-like library)338.48644.71903fatty acid desaturase (OLE1)
SGTC_10094106-00579.87 μM0.4909092928005ChemDiv (Drug-like library)443.580525.0314
SGTC_3031908876249.47 μM0.458333205253Chembridge (Drug-like library)349.254324.11302
SGTC_2300797669771.57 μM0.4565222236109Chembridge (Fragment library)238.756362.28912ERG2
SGTC_2784092-082123.9 μM0.452381877900ChemDiv (Drug-like library)328.87895.05102plasma membrane duress
SGTC_3084092-1169104 μM0.450982268590ChemDiv (Drug-like library)346.851123.8514plasma membrane duress
SGTC_3044909319349.47 μM0.42592625236277Chembridge (Drug-like library)334.863563.54803
SGTC_3139909502049.47 μM0.42592625236292Chembridge (Drug-like library)318.797962.98903
SGTC_1784092-080687.51 μM0.4255322169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_3140909424349.47 μM0.42105319291112Chembridge (Drug-like library)342.4072233.32204