4112-3290

N-(3-methylphenyl)-4-pyridin-2-yl-1,3-thiazol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1442
Screen concentration 3.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 704353
SMILES CC1=CC(=CC=C1)NC2=NC(=CS2)C3=CC=CC=N3
Standardized SMILES Cc1cccc(Nc2nc(cs2)c3ccccn3)c1
Molecular weight 267.3488
ALogP 4.09
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.07
% growth inhibition (Hom. pool) 1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 704353
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.22||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:NOP58(YOR310C)|FD-Score:3.79|P-value:7.43E-5|Clearance:0.41||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RIO1(YOR119C)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.41||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:SSL1(YLR005W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.08||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:ACT1(YFL039C)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.22||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:NOP58(YOR310C)|FD-Score:3.79|P-value:7.43E-5|Clearance:0.41||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RIO1(YOR119C)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.41||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:SSL1(YLR005W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.08||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 704353
Download HOP data (tab-delimited text)  (excel)
Gene:ADY2(YCR010C)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM46(YHR199C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALK2(YBL009W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATC1(YDR184C)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CWP1(YKL096W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance Gene:ECM34(YHL043W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:EMC1(YCL045C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:ERP5(YHR110W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ESC2(YDR363W)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FYV7(YLR068W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:HBS1(YKR084C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HOG1(YLR113W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:LRE1(YCL051W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSB6(YJL100W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MCT1(YOR221C)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM31(YHR194W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MET12(YPL023C)|FD-Score:4.32|P-value:7.98E-6||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MKK1(YOR231W)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRF1(YGL143C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:NOP12(YOL041C)|FD-Score:4.27|P-value:9.67E-6||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NVJ1(YHR195W)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OCA5(YHL029C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PES4(YFR023W)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:RAD54(YGL163C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RPL40A(YIL148W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RRF1(YHR038W)|FD-Score:4.47|P-value:3.93E-6||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:SAE3(YHR079C-A)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SCW11(YGL028C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SLX9(YGR081C)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SPE3(YPR069C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SRC1(YML034W)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STE50(YCL032W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SYF2(YGR129W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRI1(YMR233W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP5(YER144C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA13(YPR036W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS35(YJL154C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS68(YOL129W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YDL085C-A(YDL085C-A_p)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YGL088W(YGL088W_d)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YJL055W(YJL055W_p)|FD-Score:4.92|P-value:4.42E-7||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YML018C(YML018C_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR141C(YMR141C_d)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL195C(YNL195C_p)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL217W(YNL217W_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YPL182C(YPL182C_d)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:ADY2(YCR010C)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM46(YHR199C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALK2(YBL009W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATC1(YDR184C)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CWP1(YKL096W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance Gene:ECM34(YHL043W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:EMC1(YCL045C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:ERP5(YHR110W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ESC2(YDR363W)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FYV7(YLR068W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:HBS1(YKR084C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HOG1(YLR113W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:LRE1(YCL051W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSB6(YJL100W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MCT1(YOR221C)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM31(YHR194W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MET12(YPL023C)|FD-Score:4.32|P-value:7.98E-6||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MKK1(YOR231W)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRF1(YGL143C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:NOP12(YOL041C)|FD-Score:4.27|P-value:9.67E-6||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NVJ1(YHR195W)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OCA5(YHL029C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PES4(YFR023W)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:RAD54(YGL163C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RPL40A(YIL148W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RRF1(YHR038W)|FD-Score:4.47|P-value:3.93E-6||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:SAE3(YHR079C-A)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SCW11(YGL028C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SLX9(YGR081C)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SPE3(YPR069C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SRC1(YML034W)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STE50(YCL032W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SYF2(YGR129W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRI1(YMR233W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP5(YER144C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA13(YPR036W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS35(YJL154C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS68(YOL129W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YDL085C-A(YDL085C-A_p)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YGL088W(YGL088W_d)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YJL055W(YJL055W_p)|FD-Score:4.92|P-value:4.42E-7||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YML018C(YML018C_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR141C(YMR141C_d)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL195C(YNL195C_p)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL217W(YNL217W_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YPL182C(YPL182C_d)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR119C4.072.32E-50.41RIO1Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA
YOR310C3.797.43E-50.41NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YFL039C3.383.59E-40.22ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLR005W3.167.79E-40.08SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YDR238C3.090.001010.10SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YKR062W2.990.001380.04TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YOL040C2.950.001570.02RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YNL114C_d2.940.001660.02YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YGR103W2.920.001750.00NOP7Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
YDR280W2.920.001780.14RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YFR002W2.770.002780.13NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YDL111C2.640.004110.04RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YKR071C2.610.004590.12DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YGR048W2.480.006510.03UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YGL207W2.460.007000.01SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL055W_p4.924.42E-7YJL055W_pPutative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU
YFR023W4.483.67E-6PES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
YHR038W4.473.93E-6RRF1Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
YOR221C4.454.34E-6MCT1Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
YDL085C-A_p4.376.29E-6YDL085C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YPL023C4.327.98E-6MET12Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR
YOL041C4.279.67E-6NOP12Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
YCL045C4.221.21E-5EMC1Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
YLR068W4.191.37E-5FYV7Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
YGR129W4.032.74E-5SYF2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest
YKR084C3.983.44E-5HBS1GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay
YGR081C3.953.88E-5SLX9Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
YCR010C3.924.50E-5ADY2Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication
YPR036W3.904.79E-5VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YNL195C_p3.904.82E-5YNL195C_pPutative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_1442
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0993.36E-14SGTC_11931488-0126 41.5 μMChemDiv (Drug-like library)13847100.132353
0.0814.85E-10SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.134328
0.0806.78E-10SGTC_1663st014140 55.4 μMTimTec (Natural product derivative library)42071200.060975660S ribosome export
0.0791.10E-9SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0169492
0.0773.32E-9SGTC_14294049-0205 9.5 μMChemDiv (Drug-like library)227744780.126984
0.0765.14E-9SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.109091
0.0756.73E-9SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.0983607
0.0758.86E-9SGTC_15892',5'-dimethoxyflavone 47.2 μMTimTec (Pure natural product library)6886670.107692
0.0731.63E-8SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.116667
0.0706.97E-8SGTC_31729105863 49.5 μMChembridge (Drug-like library)23487850.0897436ERG2
0.0691.14E-7SGTC_2636apigenin dimethyl ether 100.0 μMMicrosource (Natural product library)52816010.0714286
0.0681.57E-7SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.144928
0.0681.97E-7SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.105263
0.0672.76E-7SGTC_24865283202 22.4 μMMiscellaneous13777510.0793651
0.0664.32E-7SGTC_2661dactinomycin 13.0 μMMicrosource (Natural product library)450061400.05redox potentiating

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8024112-3352742 μM0.625704594ChemDiv (Drug-like library)267.348823.65914
SGTC_14764112-32943.34 μM0.622222704369ChemDiv (Drug-like library)332.21834.351460S ribosome export
SGTC_14434112-330314 μM0.617021704433ChemDiv (Drug-like library)296.390043.76415
SGTC_10104112-329615.2 μM0.55102704384ChemDiv (Drug-like library)283.348223.58515
SGTC_14444112-358128 μM0.4629632877249ChemDiv (Drug-like library)303.402523.37226
SGTC_730327-032516.23 μM0.41666753298ChemDiv (Drug-like library)284.354445.38912DNA damage response
SGTC_1524469-0003551.23 μM0.4697280ChemDiv (Drug-like library)268.333584.08224
SGTC_6281636-025316 μM0.44456143ChemDiv (Drug-like library)331.230245.07213sphingolipid biosynthesis & PDR1
SGTC_2916787599368.79 μM0.3770491299250Chembridge (Drug-like library)327.3759633.6525
SGTC_15064226-2149212 μM0.359375991114ChemDiv (Drug-like library)355.844564.54115