4239-0156

N-(3,4-difluorophenyl)-3-[4-(2-methoxyphenyl)piperazin-1-yl]propanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1445
Screen concentration 97.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 4008346
SMILES COC1=CC=CC=C1N2CCN(CC2)CCC(=O)NC3=CC(=C(C=C3)F)F
Standardized SMILES COc1ccccc1N2CCN(CCC(=O)Nc3ccc(F)c(F)c3)CC2
Molecular weight 375.4123
ALogP 3.23
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.58
% growth inhibition (Hom. pool) -1.57


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4008346
Download HIP data (tab-delimited text)  (excel)
Gene:CBF2(YGR140W)|FD-Score:3.14|P-value:8.36E-4|Clearance:0.08||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.73|P-value:9.71E-5|Clearance:0.39||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CSE1(YGL238W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:GCD14(YJL125C)|FD-Score:4.4|P-value:5.39E-6|Clearance:0.09||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:HSP10(YOR020C)|FD-Score:5.03|P-value:2.51E-7|Clearance:0.62||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:PRP3(YDR473C)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.08||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:SOG2(YOR353C)|FD-Score:-4.72|P-value:1.20E-6|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:TRM112(YNR046W)|FD-Score:4.31|P-value:8.08E-6|Clearance:0.5||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR187C(YDR187C_d)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YIP1(YGR172C)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.17||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:CBF2(YGR140W)|FD-Score:3.14|P-value:8.36E-4|Clearance:0.08||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.73|P-value:9.71E-5|Clearance:0.39||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CSE1(YGL238W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:GCD14(YJL125C)|FD-Score:4.4|P-value:5.39E-6|Clearance:0.09||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:HSP10(YOR020C)|FD-Score:5.03|P-value:2.51E-7|Clearance:0.62||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:PRP3(YDR473C)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.08||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:SOG2(YOR353C)|FD-Score:-4.72|P-value:1.20E-6|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:TRM112(YNR046W)|FD-Score:4.31|P-value:8.08E-6|Clearance:0.5||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR187C(YDR187C_d)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YIP1(YGR172C)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.17||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4008346
Download HOP data (tab-delimited text)  (excel)
Gene:ADD37(YMR184W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:ALG12(YNR030W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ARG3(YJL088W)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL3(YPL051W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO10(YDR380W)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ARP6(YLR085C)|FD-Score:3.8|P-value:7.23E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BMH1(YER177W)|FD-Score:-3.9|P-value:4.86E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRE5(YNR051C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CDH1(YGL003C)|FD-Score:5|P-value:2.90E-7||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COQ10(YOL008W)|FD-Score:4.87|P-value:5.49E-7||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COQ8(YGL119W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:DFG16(YOR030W)|FD-Score:4.82|P-value:7.19E-7||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DGR2(YKL121W_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DMA1(YHR115C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DUS1(YML080W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:DYN1(YKR054C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM5(YMR176W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EXG1(YLR300W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FOX2(YKR009C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:FTR1(YER145C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GEA2(YEL022W)|FD-Score:8.11|P-value:2.58E-16||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLO1(YML004C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GSF2(YML048W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HEL2(YDR266C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:ISA2(YPR067W)|FD-Score:-4.81|P-value:7.63E-7||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISR1(YPR106W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KXD1(YGL079W_p)|FD-Score:3.78|P-value:7.86E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LOA1(YPR139C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM12(YHR121W)|FD-Score:4.39|P-value:5.68E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MDE1(YJR024C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant Gene:NAP1(YKR048C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NPL3(YDR432W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NTG2(YOL043C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:OPI1(YHL020C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PFK1(YGR240C)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGM3(YMR278W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIN3(YPR154W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PUS7(YOR243C)|FD-Score:4.78|P-value:8.83E-7||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD7(YJR052W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:REG1(YDR028C)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RML2(YEL050C)|FD-Score:6.97|P-value:1.62E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPI1(YIL119C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL26B(YGR034W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RTC3(YHR087W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT101(YJL047C)|FD-Score:-3.82|P-value:6.65E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RVS161(YCR009C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAY1(YGR263C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SDT1(YGL224C)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SGF11(YPL047W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SIT4(YDL047W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:STP1(YDR463W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TAX4(YJL083W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:THI74(YDR438W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:5.35|P-value:4.40E-8||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:USA1(YML029W)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VOA1(YGR106C)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS62(YGR141W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:WTM1(YOR230W)|FD-Score:4.94|P-value:3.81E-7||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YFH7(YFR007W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Putative protein of unknown function Gene:YHR095W(YHR095W_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIG1(YPL201C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL032C(YIL032C_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL043W(YJL043W_p)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YJL213W(YJL213W)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YJR037W(YJR037W_d)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL070W(YKL070W_p)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR078W(YKR078W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLL056C(YLL056C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YMR085W(YMR085W_p)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YPL077C(YPL077C_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:ALG12(YNR030W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ARG3(YJL088W)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL3(YPL051W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO10(YDR380W)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ARP6(YLR085C)|FD-Score:3.8|P-value:7.23E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BMH1(YER177W)|FD-Score:-3.9|P-value:4.86E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRE5(YNR051C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CDH1(YGL003C)|FD-Score:5|P-value:2.90E-7||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COQ10(YOL008W)|FD-Score:4.87|P-value:5.49E-7||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COQ8(YGL119W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:DFG16(YOR030W)|FD-Score:4.82|P-value:7.19E-7||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DGR2(YKL121W_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DMA1(YHR115C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DUS1(YML080W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:DYN1(YKR054C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM5(YMR176W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EXG1(YLR300W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FOX2(YKR009C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:FTR1(YER145C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GEA2(YEL022W)|FD-Score:8.11|P-value:2.58E-16||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLO1(YML004C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GSF2(YML048W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HEL2(YDR266C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:ISA2(YPR067W)|FD-Score:-4.81|P-value:7.63E-7||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISR1(YPR106W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KXD1(YGL079W_p)|FD-Score:3.78|P-value:7.86E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LOA1(YPR139C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM12(YHR121W)|FD-Score:4.39|P-value:5.68E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MDE1(YJR024C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant Gene:NAP1(YKR048C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NPL3(YDR432W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NTG2(YOL043C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:OPI1(YHL020C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PFK1(YGR240C)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGM3(YMR278W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIN3(YPR154W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PUS7(YOR243C)|FD-Score:4.78|P-value:8.83E-7||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD7(YJR052W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:REG1(YDR028C)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RML2(YEL050C)|FD-Score:6.97|P-value:1.62E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPI1(YIL119C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL26B(YGR034W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RTC3(YHR087W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT101(YJL047C)|FD-Score:-3.82|P-value:6.65E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RVS161(YCR009C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAY1(YGR263C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SDT1(YGL224C)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SGF11(YPL047W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SIT4(YDL047W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:STP1(YDR463W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TAX4(YJL083W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:THI74(YDR438W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:5.35|P-value:4.40E-8||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:USA1(YML029W)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VOA1(YGR106C)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS62(YGR141W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:WTM1(YOR230W)|FD-Score:4.94|P-value:3.81E-7||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YFH7(YFR007W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Putative protein of unknown function Gene:YHR095W(YHR095W_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIG1(YPL201C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL032C(YIL032C_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL043W(YJL043W_p)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YJL213W(YJL213W)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YJR037W(YJR037W_d)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL070W(YKL070W_p)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR078W(YKR078W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLL056C(YLL056C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YMR085W(YMR085W_p)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YPL077C(YPL077C_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YOR020C5.032.51E-70.62HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YJL125C4.405.39E-60.09GCD14Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YNR046W4.318.08E-60.50TRM112Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
YDR473C3.816.98E-50.08PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YAL041W3.739.71E-50.39CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDR187C_d3.344.24E-40.02YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YGR172C3.314.61E-40.17YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YGR140W3.148.36E-40.08CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YKR083C3.060.001090.04DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YDL003W3.030.001230.08MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YGR115C_d2.950.001580.08YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YMR093W2.880.002020.03UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YHR085W2.840.002250.05IPI1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
YJL015C_d2.790.002630.01YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YML049C2.780.002720.06RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL022W8.112.58E-16GEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
YEL050C6.971.62E-12RML2Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
YGR106C5.393.61E-8VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YMR156C5.354.40E-8TPP1DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
YJL088W5.121.52E-7ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YGL003C5.002.90E-7CDH1Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
YOR230W4.943.81E-7WTM1Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
YOL008W4.875.49E-7COQ10Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
YGL188C-A_p4.836.77E-7YGL188C-A_pPutative protein of unknown function
YOR030W4.827.19E-7DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
YOR243C4.788.83E-7PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YGR240C4.671.52E-6PFK1Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YBR033W_p4.621.90E-6EDS1_pPutative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication
YKR078W4.621.94E-6YKR078WCytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P)
YBR196C-A_p4.612.06E-6YBR196C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR

GO enrichment analysis for SGTC_1445
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0663.77E-7SGTC_446go6976 132.0 μMICCB bioactive library35010.101124
0.0589.82E-6SGTC_21665933061 31.5 μMChembridge (Fragment library)28773750.0684932tubulin folding & SWR complex
0.0571.14E-5SGTC_1644st010134 20.9 μMTimTec (Natural product derivative library)38181340.0759494mitochondrial processes
0.0552.31E-5SGTC_24445485484 36.4 μMMiscellaneous67906310.0759494
0.0552.58E-5SGTC_14813346-2004 36.0 μMChemDiv (Drug-like library)27705220.259259
0.0552.60E-5SGTC_8860866-0150 153.0 μMChemDiv (Drug-like library)2714630.112676
0.0543.65E-5SGTC_14853909-9022 254.0 μMChemDiv (Drug-like library)7145160.0987654RPP1 & pyrimidine depletion
0.0501.07E-4SGTC_6811642-0006 26.6 μMChemDiv (Drug-like library)44465300.134146
0.0501.14E-4SGTC_1073triclabendazole 3.1 μMNIH Clinical Collection502480.1125
0.0491.61E-4SGTC_29969066192 71.4 μMChembridge (Drug-like library)64728540.111111
0.0491.65E-4SGTC_31029121669 49.5 μMChembridge (Drug-like library)419050720.0875
0.0482.12E-4SGTC_1579chlormadinone acetate 49.4 μMMiscellaneous451142580.0879121
0.0482.30E-4SGTC_3341502-1024 68.5 μMChemDiv (Drug-like library)32962160.207792
0.0482.52E-4SGTC_7771315-0167 517.0 μMChemDiv (Drug-like library)42217670.0659341Golgi
0.0472.86E-4SGTC_21585657123 3.6 μMChembridge (Fragment library)28588270.0958904

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14464239-019062 μM0.7241381340085ChemDiv (Drug-like library)379.495263.87414
SGTC_14544239-005442.6 μM0.7241381340767ChemDiv (Drug-like library)432.354044.05214
SGTC_2939904457871.43 μM0.6779662653634Chembridge (Drug-like library)377.8403433.43915
SGTC_10174239-0030168 μM0.5862071149593ChemDiv (Drug-like library)406.2919433.78814
SGTC_3284910817164.86 μM0.5806458687505Chembridge (Drug-like library)373.876463.7214
SGTC_3202911376149.47 μM0.5468754798415Chembridge (Drug-like library)377.8403433.43915
SGTC_3208911411549.47 μM0.53125650895Chembridge (Drug-like library)357.4218633.26115
SGTC_2798781363745.45 μM0.474576956368Chembridge (Drug-like library)277.2660263.34714
SGTC_14474239-081681.9 μM0.4328361341554ChemDiv (Drug-like library)367.4597433.41814
SGTC_3130912741449.47 μM0.3835621494646Chembridge (Drug-like library)425.914564.0328