4239-0816

N-(3-fluorophenyl)-3-[4-[(E)-3-phenylprop-2-enyl]piperazin-1-yl]propanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1447
Screen concentration 81.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 1341554
SMILES C1CN(CCN1CCC(=O)NC2=CC(=CC=C2)F)CC=CC3=CC=CC=C3
Standardized SMILES Fc1cccc(NC(=O)CCN2CCN(CC=Cc3ccccc3)CC2)c1
Molecular weight 367.4597
ALogP 3.42
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7
% growth inhibition (Hom. pool) 2.58


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1341554
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.38||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:3.39|P-value:3.49E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC7(YDL017W)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p Gene:DCP1(YOL149W)|FD-Score:-3.45|P-value:2.85E-4|Clearance:0||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:HEM12(YDR047W)|FD-Score:6.02|P-value:8.65E-10|Clearance:0.44||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IRA1(YBR140C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.15||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KAP95(YLR347C)|FD-Score:5.59|P-value:1.13E-8|Clearance:0.44||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MAS1(YLR163C)|FD-Score:4.68|P-value:1.45E-6|Clearance:0.17||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD4(YKL165C)|FD-Score:4.51|P-value:3.29E-6|Clearance:0.27||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:OLE1(YGL055W)|FD-Score:3.16|P-value:7.96E-4|Clearance:0.04||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RPL10(YLR075W)|FD-Score:3.39|P-value:3.54E-4|Clearance:0.23||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL5(YPL131W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPR2(YIR015W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.02||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSC9(YML127W)|FD-Score:4.79|P-value:8.34E-7|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SPC42(YKL042W)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:STH1(YIL126W)|FD-Score:5.23|P-value:8.56E-8|Clearance:0.44||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TEN1(YLR010C)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.03||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:YJL086C(YJL086C_d)|FD-Score:5.38|P-value:3.63E-8|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR317W(YLR317W_d)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.18||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:BET4(YJL031C)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.38||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:3.39|P-value:3.49E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC7(YDL017W)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p Gene:DCP1(YOL149W)|FD-Score:-3.45|P-value:2.85E-4|Clearance:0||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:HEM12(YDR047W)|FD-Score:6.02|P-value:8.65E-10|Clearance:0.44||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IRA1(YBR140C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.15||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KAP95(YLR347C)|FD-Score:5.59|P-value:1.13E-8|Clearance:0.44||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MAS1(YLR163C)|FD-Score:4.68|P-value:1.45E-6|Clearance:0.17||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD4(YKL165C)|FD-Score:4.51|P-value:3.29E-6|Clearance:0.27||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:OLE1(YGL055W)|FD-Score:3.16|P-value:7.96E-4|Clearance:0.04||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RPL10(YLR075W)|FD-Score:3.39|P-value:3.54E-4|Clearance:0.23||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL5(YPL131W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPR2(YIR015W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.02||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSC9(YML127W)|FD-Score:4.79|P-value:8.34E-7|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SPC42(YKL042W)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:STH1(YIL126W)|FD-Score:5.23|P-value:8.56E-8|Clearance:0.44||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TEN1(YLR010C)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.03||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:YJL086C(YJL086C_d)|FD-Score:5.38|P-value:3.63E-8|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR317W(YLR317W_d)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.18||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1341554
Download HOP data (tab-delimited text)  (excel)
Gene:ARO2(YGL148W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG2(YNL242W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATG33(YLR356W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:AXL1(YPR122W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BCK1(YJL095W)|FD-Score:9.53|P-value:7.90E-22||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIO4(YNR057C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BPH1(YCR032W)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BRE5(YNR051C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:COQ8(YGL119W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:DHH1(YDL160C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:ECI1(YLR284C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EIS1(YMR031C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ENV10(YLR065C)|FD-Score:8.15|P-value:1.76E-16||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERV46(YAL042W)|FD-Score:-4.7|P-value:1.29E-6||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FCY22(YER060W-A)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FET4(YMR319C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE8(YLR047C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GDB1(YPR184W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HHT1(YBR010W)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HOP1(YIL072W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HOS1(YPR068C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:ICS2(YBR157C)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IKI3(YLR384C)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IRS4(YKR019C)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JNM1(YMR294W)|FD-Score:5.01|P-value:2.78E-7||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KRE6(YPR159W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LSC1(YOR142W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MBP1(YDL056W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MET12(YPL023C)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MET28(YIR017C)|FD-Score:4.79|P-value:8.18E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MRPL27(YBR282W)|FD-Score:4.83|P-value:6.86E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPS5(YBR251W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NCS2(YNL119W)|FD-Score:-4.69|P-value:1.36E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NHP6B(YBR089C-A)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:PFK2(YMR205C)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHR1(YOR386W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:POR1(YNL055C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:5.38|P-value:3.74E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY1(YJL079C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RDS1(YCR106W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RGD1(YBR260C)|FD-Score:4.77|P-value:9.04E-7||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RTT109(YLL002W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAC3(YDR159W)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SLF1(YDR515W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPO19(YPL130W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:SPS18(YNL204C)|FD-Score:4.98|P-value:3.10E-7||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPT4(YGR063C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRB8(YCR081W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSF2(YDR312W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:THI6(YPL214C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TIM8(YJR135W-A)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRP2(YER090W)|FD-Score:5.81|P-value:3.16E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.27|P-value:7.00E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UIP4(YPL186C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA13(YPR036W)|FD-Score:-4.38|P-value:5.93E-6||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA9(YCL005W-A)|FD-Score:4.82|P-value:7.22E-7||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS72(YDR485C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:WHI3(YNL197C)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL083C(YBL083C_d)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR032W(YBR032W_d)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDL009C(YDL009C_p)|FD-Score:5.8|P-value:3.39E-9||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.53|P-value:1.60E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR445C(YDR445C_d)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER137C(YER137C_p)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Putative protein of unknown function Gene:YGR069W(YGR069W_d)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL043W(YJL043W_p)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YKR015C(YKR015C_p)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function Gene:YKR073C(YKR073C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR252W(YLR252W_d)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL010W(YNL010W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR131C(YOR131C_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR139C(YOR139C_d)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPK3(YBR028C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YSP1(YHR155W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ARO2(YGL148W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG2(YNL242W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATG33(YLR356W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:AXL1(YPR122W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BCK1(YJL095W)|FD-Score:9.53|P-value:7.90E-22||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIO4(YNR057C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BPH1(YCR032W)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BRE5(YNR051C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:COQ8(YGL119W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:DHH1(YDL160C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:ECI1(YLR284C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EIS1(YMR031C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ENV10(YLR065C)|FD-Score:8.15|P-value:1.76E-16||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERV46(YAL042W)|FD-Score:-4.7|P-value:1.29E-6||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FCY22(YER060W-A)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FET4(YMR319C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE8(YLR047C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GDB1(YPR184W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HHT1(YBR010W)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HOP1(YIL072W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HOS1(YPR068C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:ICS2(YBR157C)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IKI3(YLR384C)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IRS4(YKR019C)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JNM1(YMR294W)|FD-Score:5.01|P-value:2.78E-7||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KRE6(YPR159W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LSC1(YOR142W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MBP1(YDL056W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MET12(YPL023C)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MET28(YIR017C)|FD-Score:4.79|P-value:8.18E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MRPL27(YBR282W)|FD-Score:4.83|P-value:6.86E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPS5(YBR251W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NCS2(YNL119W)|FD-Score:-4.69|P-value:1.36E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NHP6B(YBR089C-A)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:PFK2(YMR205C)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHR1(YOR386W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:POR1(YNL055C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:5.38|P-value:3.74E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY1(YJL079C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RDS1(YCR106W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RGD1(YBR260C)|FD-Score:4.77|P-value:9.04E-7||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RTT109(YLL002W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAC3(YDR159W)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SLF1(YDR515W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPO19(YPL130W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:SPS18(YNL204C)|FD-Score:4.98|P-value:3.10E-7||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPT4(YGR063C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRB8(YCR081W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSF2(YDR312W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:THI6(YPL214C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TIM8(YJR135W-A)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRP2(YER090W)|FD-Score:5.81|P-value:3.16E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.27|P-value:7.00E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UIP4(YPL186C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA13(YPR036W)|FD-Score:-4.38|P-value:5.93E-6||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA9(YCL005W-A)|FD-Score:4.82|P-value:7.22E-7||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS72(YDR485C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:WHI3(YNL197C)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL083C(YBL083C_d)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR032W(YBR032W_d)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDL009C(YDL009C_p)|FD-Score:5.8|P-value:3.39E-9||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.53|P-value:1.60E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR445C(YDR445C_d)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER137C(YER137C_p)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Putative protein of unknown function Gene:YGR069W(YGR069W_d)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL043W(YJL043W_p)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YKR015C(YKR015C_p)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function Gene:YKR073C(YKR073C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR252W(YLR252W_d)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL010W(YNL010W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR131C(YOR131C_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR139C(YOR139C_d)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPK3(YBR028C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YSP1(YHR155W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W6.028.65E-100.44HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YLR347C5.591.13E-80.44KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YJL086C_d5.383.63E-80.44YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YIL126W5.238.56E-80.44STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YML127W4.798.34E-70.11RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YLR163C4.681.45E-60.17MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YKL165C4.513.29E-60.27MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YPL131W4.231.15E-50.14RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YLR317W_d4.102.08E-50.18YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
YJL031C3.924.51E-50.38BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YBR140C3.542.00E-40.15IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YGL116W3.393.49E-40.00CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YLR075W3.393.54E-40.23RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YGL055W3.167.96E-40.04OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YIR015W3.129.13E-40.02RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL095W9.537.90E-22BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YLR065C8.151.76E-16ENV10Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
YER090W5.813.16E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDL009C_p5.803.39E-9YDL009C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
YDR008C_d5.531.60E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER137C_p5.432.89E-8YER137C_pPutative protein of unknown function
YDR159W5.423.02E-8SAC3Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
YOR323C5.383.74E-8PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YKL211C5.277.00E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YMR294W5.012.78E-7JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YNL204C4.983.10E-7SPS18Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation
YBR028C4.963.51E-7YPK3An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner
YOR139C_d4.895.05E-7YOR139C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W
YBR282W4.836.86E-7MRPL27Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
YCL005W-A4.827.22E-7VMA9Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis

GO enrichment analysis for SGTC_1447
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1701.55E-39SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.145161plasma membrane duress
0.1695.68E-39SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.141176
0.1631.93E-36SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.121622cell wall signaling
0.1597.12E-35SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.113924
0.1507.40E-31SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.078125
0.1483.10E-30SGTC_2725haloperidol 53.2 μMMiscellaneous35590.22973plasma membrane duress
0.1476.18E-30SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.128205
0.1477.83E-30SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.0857143
0.1403.39E-27SGTC_20034023581 200.0 μMChembridge (Fragment library)166413070.119403cell wall signaling
0.1374.85E-26SGTC_11604092-0570 10.5 μMChemDiv (Drug-like library)49949750.151899plasma membrane duress
0.1366.83E-26SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.111111cell wall signaling
0.1361.31E-25SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0365854
0.1301.09E-23SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0416667
0.1293.28E-23SGTC_10114112-3381 17.4 μMChemDiv (Drug-like library)28771390.0689655
0.1286.70E-23SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.1125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2896905265671.43 μM0.6140356469436Chembridge (Drug-like library)369.887763.62913endomembrane recycling
SGTC_14454239-015697.7 μM0.4328364008346ChemDiv (Drug-like library)375.4123263.22816
SGTC_10174239-0030168 μM0.4285711149593ChemDiv (Drug-like library)406.2919433.78814
SGTC_2977901119771.43 μM0.3965522704722Chembridge (Drug-like library)279.3050463.41914
SGTC_12810884-003111.8 μM0.389831548255ChemDiv (Drug-like library)366.498045.21202fatty acid desaturase (OLE1)
SGTC_2880904298358.44 μM0.3846156464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_3282910849815.46 μM0.38235316459645Chembridge (Drug-like library)280.2947831.4215
SGTC_14464239-019062 μM0.3802821340085ChemDiv (Drug-like library)379.495263.87414
SGTC_14544239-005442.6 μM0.3802821340767ChemDiv (Drug-like library)432.354044.05214
SGTC_8870885-00079.62 μM0.3484855719458ChemDiv (Drug-like library)425.565245.16513endomembrane recycling