k072-0203

1-(3-phenoxyphenyl)-N-(1,2,4-triazol-4-yl)methanimine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1461
Screen concentration 53.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 5418714
SMILES C1=CC=C(C=C1)OC2=CC=CC(=C2)C=NN3C=NN=C3
Standardized SMILES O(c1ccccc1)c2cccc(C=Nn3cnnc3)c2
Molecular weight 264.282
ALogP 2.61
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.9
% growth inhibition (Hom. pool) 6.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5418714
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:-3.9|P-value:4.80E-5|Clearance:0||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ACT1(YFL039C)|FD-Score:3.79|P-value:7.64E-5|Clearance:0.02||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AOS1(YPR180W)|FD-Score:-8.08|P-value:3.32E-16|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CCT5(YJR064W)|FD-Score:4.87|P-value:5.71E-7|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CEP3(YMR168C)|FD-Score:4.75|P-value:1.01E-6|Clearance:0.04||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:ERG12(YMR208W)|FD-Score:5.08|P-value:1.93E-7|Clearance:0.21||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MIA40(YKL195W)|FD-Score:4.67|P-value:1.49E-6|Clearance:0.89||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MPS3(YJL019W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.3||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:RLP24(YLR009W)|FD-Score:3.77|P-value:8.22E-5|Clearance:0.15||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPN3(YER021W)|FD-Score:8.13|P-value:2.07E-16|Clearance:3.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:STH1(YIL126W)|FD-Score:3.13|P-value:8.68E-4|Clearance:0.16||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TOM40(YMR203W)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.04||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL196W(YDL196W_d)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ABF1(YKL112W)|FD-Score:-3.9|P-value:4.80E-5|Clearance:0||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ACT1(YFL039C)|FD-Score:3.79|P-value:7.64E-5|Clearance:0.02||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AOS1(YPR180W)|FD-Score:-8.08|P-value:3.32E-16|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CCT5(YJR064W)|FD-Score:4.87|P-value:5.71E-7|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CEP3(YMR168C)|FD-Score:4.75|P-value:1.01E-6|Clearance:0.04||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:ERG12(YMR208W)|FD-Score:5.08|P-value:1.93E-7|Clearance:0.21||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MIA40(YKL195W)|FD-Score:4.67|P-value:1.49E-6|Clearance:0.89||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MPS3(YJL019W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.3||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:RLP24(YLR009W)|FD-Score:3.77|P-value:8.22E-5|Clearance:0.15||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPN3(YER021W)|FD-Score:8.13|P-value:2.07E-16|Clearance:3.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:STH1(YIL126W)|FD-Score:3.13|P-value:8.68E-4|Clearance:0.16||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TOM40(YMR203W)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.04||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL196W(YDL196W_d)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5418714
Download HOP data (tab-delimited text)  (excel)
Gene:AIM10(YER087W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ATC1(YDR184C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG27(YJL178C)|FD-Score:-3.25|P-value:5.73E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BNI5(YNL166C)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:BPH1(YCR032W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CIN8(YEL061C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CRR1(YLR213C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation Gene:DAL4(YIR028W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:-4.92|P-value:4.29E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EFM1(YHL039W)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ETT1(YOR051C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:GCS1(YDL226C)|FD-Score:-4.43|P-value:4.71E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEP4(YHR100C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIS3(YLR094C)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Protein of unknown function Gene:INP51(YIL002C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:ITC1(YGL133W)|FD-Score:-3.84|P-value:6.06E-5||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:MEF1(YLR069C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM101(YJR144W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MIP1(YOR330C)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MND2(YIR025W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPS17(YMR188C)|FD-Score:4.97|P-value:3.36E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS5(YBR251W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRT4(YKL009W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSH5(YDL154W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NUP120(YKL057C)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:PIF1(YML061C)|FD-Score:5.21|P-value:9.56E-8||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:POP2(YNR052C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPT1(YGR123C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:PSO2(YMR137C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RDR1(YOR380W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RIB4(YOL143C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIM1(YCR028C-A)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RML2(YEL050C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPN4(YDL020C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SRL3(YKR091W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SUT1(YGL162W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TGS1(YPL157W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPO3(YPR156C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP2(YER090W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UPF3(YGR072W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VEL1(YGL258W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:YDL176W(YDL176W)|FD-Score:-5.39|P-value:3.44E-8||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR521W(YDR521W_d)|FD-Score:-3.81|P-value:7.05E-5||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YET3(YDL072C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGR022C(YGR022C_d)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Gene:YGR107W(YGR107W_d)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL060W(YIL060W_p)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJL022W(YJL022W_d)|FD-Score:-4.57|P-value:2.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJL171C(YJL171C)|FD-Score:-4.61|P-value:2.03E-6||SGD DESC:GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress Gene:YKL044W(YKL044W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLL054C(YLL054C_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR361C-A(YLR361C-A_p)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR074C(YMR074C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR196W(YMR196W_p)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOR050C(YOR050C_d)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR225W(YOR225W_d)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AIM10(YER087W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ATC1(YDR184C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG27(YJL178C)|FD-Score:-3.25|P-value:5.73E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BNI5(YNL166C)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:BPH1(YCR032W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CIN8(YEL061C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CRR1(YLR213C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation Gene:DAL4(YIR028W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:-4.92|P-value:4.29E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EFM1(YHL039W)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ETT1(YOR051C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:GCS1(YDL226C)|FD-Score:-4.43|P-value:4.71E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEP4(YHR100C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIS3(YLR094C)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Protein of unknown function Gene:INP51(YIL002C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:ITC1(YGL133W)|FD-Score:-3.84|P-value:6.06E-5||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:MEF1(YLR069C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM101(YJR144W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MIP1(YOR330C)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MND2(YIR025W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPS17(YMR188C)|FD-Score:4.97|P-value:3.36E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS5(YBR251W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRT4(YKL009W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSH5(YDL154W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NUP120(YKL057C)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:PIF1(YML061C)|FD-Score:5.21|P-value:9.56E-8||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:POP2(YNR052C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPT1(YGR123C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:PSO2(YMR137C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RDR1(YOR380W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RIB4(YOL143C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIM1(YCR028C-A)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RML2(YEL050C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPN4(YDL020C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SRL3(YKR091W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SUT1(YGL162W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TGS1(YPL157W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPO3(YPR156C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP2(YER090W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UPF3(YGR072W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VEL1(YGL258W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:YDL176W(YDL176W)|FD-Score:-5.39|P-value:3.44E-8||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR521W(YDR521W_d)|FD-Score:-3.81|P-value:7.05E-5||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YET3(YDL072C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGR022C(YGR022C_d)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Gene:YGR107W(YGR107W_d)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL060W(YIL060W_p)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJL022W(YJL022W_d)|FD-Score:-4.57|P-value:2.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJL171C(YJL171C)|FD-Score:-4.61|P-value:2.03E-6||SGD DESC:GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress Gene:YKL044W(YKL044W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLL054C(YLL054C_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR361C-A(YLR361C-A_p)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR074C(YMR074C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR196W(YMR196W_p)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOR050C(YOR050C_d)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR225W(YOR225W_d)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER021W8.132.07E-163.06RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YMR208W5.081.93E-70.21ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YJR064W4.875.71E-70.11CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YMR168C4.751.01E-60.04CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YMR203W4.721.20E-60.04TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YKL195W4.671.49E-60.89MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YFL039C3.797.64E-50.02ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLR009W3.778.22E-50.15RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YJL019W3.621.47E-40.30MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YDL196W_d3.324.45E-40.19YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YIL126W3.138.68E-40.16STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YDL147W2.970.001470.12RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YPR016C2.850.002170.19TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YLL018C2.660.003890.06DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YDL217C2.600.004630.01TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML061C5.219.56E-8PIF1DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
YMR188C4.973.36E-7MRPS17Mitochondrial ribosomal protein of the small subunit
YML122C_d4.924.41E-7YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR056W4.691.39E-6TRM2tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair
YIR025W4.503.33E-6MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YHL039W4.483.66E-6EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YPL157W4.082.26E-5TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YIL002C4.032.76E-5INP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
YER090W4.013.02E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YMR196W_p3.963.82E-5YMR196W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
YLR094C3.944.16E-5GIS3Protein of unknown function
YDR159W3.934.17E-5SAC3Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
YEL050C3.855.96E-5RML2Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
YDR184C3.826.69E-5ATC1Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
YKR091W3.807.35E-5SRL3Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate

GO enrichment analysis for SGTC_1461
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2211.52E-64SGTC_1462k081-0014 26.5 μMChemDiv (Drug-like library)32706340.0757576
0.2111.12E-58SGTC_1463k081-0032 34.1 μMChemDiv (Drug-like library)9777550.107692
0.1561.41E-32SGTC_264promethazine 12.5 mMMiscellaneous60140.0877193DNA intercalators
0.1524.18E-31SGTC_1952st077036 64.1 μMTimTec (Natural product derivative library)29370070.0694444
0.1451.91E-28SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0571429
0.1423.11E-27SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.107143
0.1363.43E-25SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)5150859NA
0.1357.84E-25SGTC_1457k015-0032 57.3 μMChemDiv (Drug-like library)43026600.0833333
0.1252.08E-21SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.131148
0.1243.38E-21SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0166667
0.1243.58E-21SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.0641026
0.1238.22E-21SGTC_2727riluzole 51.8 μMMiscellaneous50700.122807mitochondrial processes
0.1231.03E-20SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.0757576DNA intercalators
0.1221.11E-20SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0545455TRP & mitochondrial translation
0.1213.74E-20SGTC_14534237-0541 195.0 μMChemDiv (Drug-like library)7398920.0909091heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_155k072-020243.6 μM0.4230775857737ChemDiv (Drug-like library)332.352644.63825
SGTC_13792144-075536.5 μM0.4074075392327ChemDiv (Drug-like library)330.7440833.48205
SGTC_6690108-002126.2 μM0.32517201ChemDiv (Drug-like library)292.331843.45724fatty acid desaturase (OLE1)
SGTC_577r071-000338.6 μM0.2888894568517ChemDiv (Drug-like library)276.332443.41523fatty acid desaturase (OLE1)
SGTC_464bw-b 70c158 μM0.2786895353454ICCB bioactive library316.3268833.37124
SGTC_1030k029-004236.7 μM0.2727276080581ChemDiv (Drug-like library)341.205224.05813TSC3-RPN4
SGTC_9903985-001141.3 μM0.2678575399565ChemDiv (Drug-like library)266.297862.97514amide catabolism
SGTC_480382-001855 μM0.26762596ChemDiv (Drug-like library)231.204163.04214
SGTC_15101123-020071.5 μM0.265378812ChemDiv (Drug-like library)313.392365.39602
SGTC_80354-000170 μM0.2456144993152ChemDiv (Drug-like library)354.229326.15902