k213-0056

3-(2-chlorophenyl)-5-methyl-N-[4-(phenylcarbamoyl)phenyl]-1,2-oxazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1465
Screen concentration 144.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 24048857
SMILES CC1=C(C(=NO1)C2=CC=CC=C2Cl)C(=O)NC3=CC=C(C=C3)C(=O)NC4=CC=CC=C4
Standardized SMILES Cc1onc(c2ccccc2Cl)c1C(=O)Nc3ccc(cc3)C(=O)Nc4ccccc4
Molecular weight 431.871
ALogP 4.61
H-bond donor count 2
H-bond acceptor count 4
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.97
% growth inhibition (Hom. pool) -1.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24048857
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC20(YGL116W)|FD-Score:4.29|P-value:8.92E-6|Clearance:0.3||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG12(YMR208W)|FD-Score:11.6|P-value:1.32E-31|Clearance:6.03||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:4.85|P-value:6.22E-7|Clearance:0.4||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCR1(YPL075W)|FD-Score:3.89|P-value:4.92E-5|Clearance:0.15||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GPI8(YDR331W)|FD-Score:4.45|P-value:4.33E-6|Clearance:0.11||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HEM4(YOR278W)|FD-Score:3.74|P-value:9.20E-5|Clearance:0.13||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:IMP4(YNL075W)|FD-Score:5.61|P-value:1.00E-8|Clearance:0.35||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:MRS6(YOR370C)|FD-Score:-3.53|P-value:2.07E-4|Clearance:0||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:MSL5(YLR116W)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:PMI40(YER003C)|FD-Score:3.92|P-value:4.41E-5|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PSF2(YJL072C)|FD-Score:-3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPB5(YBR154C)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC31(YNL151C)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C31 Gene:RPG1(YBR079C)|FD-Score:3.99|P-value:3.30E-5|Clearance:0.02||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPL18A(YOL120C)|FD-Score:5.26|P-value:7.19E-8|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS2(YGL123W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.17||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:4.33|P-value:7.34E-6|Clearance:0.04||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP9(YPR137W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.11||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RRS1(YOR294W)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.11||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RVB1(YDR190C)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.01||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAD1(YFR005C)|FD-Score:3.23|P-value:6.08E-4|Clearance:0.04||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SPB1(YCL054W)|FD-Score:3.97|P-value:3.65E-5|Clearance:0.05||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SRB7(YDR308C)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.09||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SRP68(YPL243W)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:SSL1(YLR005W)|FD-Score:5.26|P-value:7.36E-8|Clearance:0.41||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFC6(YDR362C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.09||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:3.97|P-value:3.58E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:ARC19(YKL013C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC20(YGL116W)|FD-Score:4.29|P-value:8.92E-6|Clearance:0.3||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG12(YMR208W)|FD-Score:11.6|P-value:1.32E-31|Clearance:6.03||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:4.85|P-value:6.22E-7|Clearance:0.4||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCR1(YPL075W)|FD-Score:3.89|P-value:4.92E-5|Clearance:0.15||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GPI8(YDR331W)|FD-Score:4.45|P-value:4.33E-6|Clearance:0.11||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HEM4(YOR278W)|FD-Score:3.74|P-value:9.20E-5|Clearance:0.13||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:IMP4(YNL075W)|FD-Score:5.61|P-value:1.00E-8|Clearance:0.35||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:MRS6(YOR370C)|FD-Score:-3.53|P-value:2.07E-4|Clearance:0||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:MSL5(YLR116W)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:PMI40(YER003C)|FD-Score:3.92|P-value:4.41E-5|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PSF2(YJL072C)|FD-Score:-3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPB5(YBR154C)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC31(YNL151C)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C31 Gene:RPG1(YBR079C)|FD-Score:3.99|P-value:3.30E-5|Clearance:0.02||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPL18A(YOL120C)|FD-Score:5.26|P-value:7.19E-8|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS2(YGL123W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.17||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:4.33|P-value:7.34E-6|Clearance:0.04||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP9(YPR137W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.11||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RRS1(YOR294W)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.11||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RVB1(YDR190C)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.01||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAD1(YFR005C)|FD-Score:3.23|P-value:6.08E-4|Clearance:0.04||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SPB1(YCL054W)|FD-Score:3.97|P-value:3.65E-5|Clearance:0.05||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SRB7(YDR308C)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.09||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SRP68(YPL243W)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:SSL1(YLR005W)|FD-Score:5.26|P-value:7.36E-8|Clearance:0.41||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFC6(YDR362C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.09||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:3.97|P-value:3.58E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24048857
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE16(YLR028C)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ARE2(YNR019W)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ASI1(YMR119W)|FD-Score:3.73|P-value:9.48E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:AXL2(YIL140W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BCK1(YJL095W)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM4(YPL161C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BFR1(YOR198C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:CAP2(YIL034C)|FD-Score:5.47|P-value:2.21E-8||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CGI121(YML036W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CHS6(YJL099W)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CLB1(YGR108W)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG1(YGL223C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CPR7(YJR032W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTK1(YKL139W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DIC1(YLR348C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DSF2(YBR007C_p)|FD-Score:6.34|P-value:1.13E-10||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FCY22(YER060W-A)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FOX2(YKR009C)|FD-Score:-4.29|P-value:9.04E-6||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GLO3(YER122C)|FD-Score:7.25|P-value:2.11E-13||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GRR1(YJR090C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOG1(YLR113W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HOP1(YIL072W)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:IRC6(YFR043C)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:JNM1(YMR294W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAP120(YPL125W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KEX1(YGL203C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KSP1(YHR082C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LDB7(YBL006C)|FD-Score:-5.62|P-value:9.42E-9||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LOA1(YPR139C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MAD3(YJL013C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MEC3(YLR288C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MED1(YPR070W)|FD-Score:7.17|P-value:3.83E-13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MMS22(YLR320W)|FD-Score:5.68|P-value:6.67E-9||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MOD5(YOR274W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRH4(YGL064C)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRS2(YOR334W)|FD-Score:-4.5|P-value:3.33E-6||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MTC7(YEL033W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MTG2(YHR168W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:MTO1(YGL236C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:MTQ2(YDR140W)|FD-Score:4.92|P-value:4.34E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:PAU2(YEL049W)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDR8(YLR266C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PET494(YNR045W)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PHO86(YJL117W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PSK2(YOL045W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RPS12(YOR369C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RTC3(YHR087W)|FD-Score:-4.38|P-value:6.06E-6||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFP1(YLR403W)|FD-Score:5|P-value:2.89E-7||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SNC1(YAL030W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNF8(YPL002C)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:STV1(YMR054W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SWA2(YDR320C)|FD-Score:4.27|P-value:9.82E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TCA17(YEL048C)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TMT1(YER175C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TUS1(YLR425W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UAF30(YOR295W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UBP14(YBR058C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP3(YER151C)|FD-Score:5.28|P-value:6.33E-8||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:URE2(YNL229C)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS20(YMR077C)|FD-Score:4.32|P-value:7.90E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS41(YDR080W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YCF1(YDR135C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDJ1(YNL064C)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YFR056C(YFR056C_d)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGR228W(YGR228W_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIP4(YGL198W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL118W(YKL118W_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR278C(YLR278C_p)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YMD8(YML038C)|FD-Score:-3.77|P-value:8.14E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML009C-A(YML009C-A_d)|FD-Score:4.77|P-value:9.08E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR131C(YOR131C_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR345C(YOR345C_d)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:ADE1(YAR015W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE16(YLR028C)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ARE2(YNR019W)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ASI1(YMR119W)|FD-Score:3.73|P-value:9.48E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:AXL2(YIL140W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BCK1(YJL095W)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM4(YPL161C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BFR1(YOR198C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:CAP2(YIL034C)|FD-Score:5.47|P-value:2.21E-8||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CGI121(YML036W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CHS6(YJL099W)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CLB1(YGR108W)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG1(YGL223C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CPR7(YJR032W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTK1(YKL139W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DIC1(YLR348C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DSF2(YBR007C_p)|FD-Score:6.34|P-value:1.13E-10||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FCY22(YER060W-A)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FOX2(YKR009C)|FD-Score:-4.29|P-value:9.04E-6||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GLO3(YER122C)|FD-Score:7.25|P-value:2.11E-13||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GRR1(YJR090C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOG1(YLR113W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HOP1(YIL072W)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:IRC6(YFR043C)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:JNM1(YMR294W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAP120(YPL125W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KEX1(YGL203C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KSP1(YHR082C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LDB7(YBL006C)|FD-Score:-5.62|P-value:9.42E-9||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LOA1(YPR139C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MAD3(YJL013C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MEC3(YLR288C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MED1(YPR070W)|FD-Score:7.17|P-value:3.83E-13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MMS22(YLR320W)|FD-Score:5.68|P-value:6.67E-9||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MOD5(YOR274W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRH4(YGL064C)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRS2(YOR334W)|FD-Score:-4.5|P-value:3.33E-6||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MTC7(YEL033W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MTG2(YHR168W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:MTO1(YGL236C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:MTQ2(YDR140W)|FD-Score:4.92|P-value:4.34E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:PAU2(YEL049W)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDR8(YLR266C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PET494(YNR045W)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PHO86(YJL117W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PSK2(YOL045W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RPS12(YOR369C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RTC3(YHR087W)|FD-Score:-4.38|P-value:6.06E-6||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFP1(YLR403W)|FD-Score:5|P-value:2.89E-7||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SNC1(YAL030W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNF8(YPL002C)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:STV1(YMR054W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SWA2(YDR320C)|FD-Score:4.27|P-value:9.82E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TCA17(YEL048C)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TMT1(YER175C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TUS1(YLR425W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UAF30(YOR295W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UBP14(YBR058C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP3(YER151C)|FD-Score:5.28|P-value:6.33E-8||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:URE2(YNL229C)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS20(YMR077C)|FD-Score:4.32|P-value:7.90E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS41(YDR080W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YCF1(YDR135C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDJ1(YNL064C)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YFR056C(YFR056C_d)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGR228W(YGR228W_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIP4(YGL198W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL118W(YKL118W_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR278C(YLR278C_p)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YMD8(YML038C)|FD-Score:-3.77|P-value:8.14E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML009C-A(YML009C-A_d)|FD-Score:4.77|P-value:9.08E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR131C(YOR131C_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR345C(YOR345C_d)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR208W11.601.32E-316.03ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YNL075W5.611.00E-80.35IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YOL120C5.267.19E-80.00RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YLR005W5.267.36E-80.41SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YJL085W4.856.22E-70.40EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR331W4.454.33E-60.12GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YHL015W4.337.34E-60.04RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YGL116W4.298.92E-60.30CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YBR079C3.993.30E-50.02RPG1eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
YDR429C3.973.58E-50.00TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YCL054W3.973.65E-50.05SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YER003C3.924.41E-50.03PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YPL075W3.894.92E-50.15GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YOR278W3.749.20E-50.13HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YBR154C3.611.51E-40.04RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER122C7.252.11E-13GLO3ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p
YPR070W7.173.83E-13MED1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YBR007C_p6.341.13E-10DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YLR320W5.686.67E-9MMS22Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
YJL099W5.501.88E-8CHS6Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
YIL034C5.472.21E-8CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YER151C5.286.33E-8UBP3Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
YLR403W5.002.89E-7SFP1Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
YJR090C4.953.63E-7GRR1F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification
YDR140W4.924.34E-7MTQ2S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
YNL229C4.885.25E-7URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YML009C-A_d4.779.08E-7YML009C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR266C4.621.96E-6PDR8Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication
YJL117W4.454.33E-6PHO86Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
YMR077C4.327.90E-6VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes

GO enrichment analysis for SGTC_1465
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2267.33E-69SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.09375DNA intercalators
0.1339.87E-25SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.111111DNA intercalators
0.1262.78E-22SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.045977DNA intercalators
0.1258.45E-22SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.109091DNA intercalators
0.1251.10E-21SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.144737DNA intercalators
0.1232.95E-21SGTC_6330437-0093 97.7 μMChemDiv (Drug-like library)28311180.146067DNA intercalators
0.1173.09E-19SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.0941176amide catabolism
0.1111.83E-17SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.135802translation
0.1089.66E-17SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.147541DNA intercalators
0.1032.67E-15SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.121212DNA intercalators
0.1001.25E-14SGTC_469w7 172.0 μMICCB bioactive library451155700.132353ERG2
0.0952.84E-13SGTC_2000st075254 36.4 μMTimTec (Natural product derivative library)172341750.11828
0.0904.82E-12SGTC_512prima-1 270.0 μMICCB bioactive library3229680.030303DNA intercalators
0.0872.77E-11SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.130435DNA intercalators
0.0873.05E-11SGTC_711k292-0785 179.0 μMChemDiv (Drug-like library)41463150.0666667DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1466k213-007957.5 μM0.8936173657981ChemDiv (Drug-like library)445.89765.09624
SGTC_625k213-0058121 μM0.7924533655420ChemDiv (Drug-like library)476.868584.50426translation
SGTC_3148909789349.47 μM0.41269817174100Chembridge (Drug-like library)322.357823.63114
SGTC_3203911354349.47 μM0.37517018012Chembridge (Drug-like library)241.28513.08412
SGTC_29087930301100 μM0.3725492223178Chembridge (Drug-like library)387.431143.00533
SGTC_1210557-049561.34 μM0.353104427ChemDiv (Drug-like library)436.481884.25923calcium & mitochondrial duress
SGTC_5700987-006562.6 μM0.353104428ChemDiv (Drug-like library)436.481884.2592360S ribosome export
SGTC_10154130-452582 μM0.3492061363237ChemDiv (Drug-like library)392.834983.8032360S ribosome export
SGTC_8480368-008985.6 μM0.344828762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis
SGTC_10553448-067286 μM0.344828748378ChemDiv (Drug-like library)302.369723.92412