k284-1082

4-(thiophen-2-ylmethylamino)-1H-quinazoline-2-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1467
Screen concentration 293.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3334705
SMILES C1=CC=C2C(=C1)C(=NC(=S)N2)NCC3=CC=CS3
Standardized SMILES Sc1nc(NCc2cccs2)c3ccccc3n1
Molecular weight 273.3765
ALogP 3.52
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.86
% growth inhibition (Hom. pool) 2.34


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3334705
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.73|P-value:9.39E-5|Clearance:0.58||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT7(YJL111W)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.58||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC31(YOR257W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.58||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:EBP2(YKL172W)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.58||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ERG7(YHR072W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.58||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:ERG9(YHR190W)|FD-Score:4.35|P-value:6.85E-6|Clearance:0.58||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FMN1(YDR236C)|FD-Score:4|P-value:3.21E-5|Clearance:0.58||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLC7(YER133W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.58||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRA1(YBR140C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.58||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:RPN6(YDL097C)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SEC4(YFL005W)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.58||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:TAH18(YPR048W)|FD-Score:4.3|P-value:8.48E-6|Clearance:0.58||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TFB2(YPL122C)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.58||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:YKL111C(YKL111C_d)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.58||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YNL247W(YNL247W)|FD-Score:4.86|P-value:5.82E-7|Clearance:0.58||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:ARP9(YMR033W)|FD-Score:3.73|P-value:9.39E-5|Clearance:0.58||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT7(YJL111W)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.58||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC31(YOR257W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.58||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:EBP2(YKL172W)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.58||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ERG7(YHR072W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.58||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:ERG9(YHR190W)|FD-Score:4.35|P-value:6.85E-6|Clearance:0.58||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FMN1(YDR236C)|FD-Score:4|P-value:3.21E-5|Clearance:0.58||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLC7(YER133W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.58||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRA1(YBR140C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.58||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:RPN6(YDL097C)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SEC4(YFL005W)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.58||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:TAH18(YPR048W)|FD-Score:4.3|P-value:8.48E-6|Clearance:0.58||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TFB2(YPL122C)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.58||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:YKL111C(YKL111C_d)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.58||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YNL247W(YNL247W)|FD-Score:4.86|P-value:5.82E-7|Clearance:0.58||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3334705
Download HOP data (tab-delimited text)  (excel)
Gene:AIM36(YMR157C)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALE1(YOR175C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APA1(YCL050C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:ATG15(YCR068W)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BAP2(YBR068C)|FD-Score:4.29|P-value:8.87E-6||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BEM4(YPL161C)|FD-Score:6.42|P-value:6.89E-11||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA5(YLR231C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BOI2(YER114C)|FD-Score:4.88|P-value:5.38E-7||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BUD21(YOR078W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CIR2(YOR356W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CTF19(YPL018W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CUP2(YGL166W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:CWH43(YCR017C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DSE1(YER124C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSK2(YMR276W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:DUN1(YDL101C)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ERG2(YMR202W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FYV5(YCL058C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAL11(YOL051W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GIR2(YDR152W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLC3(YEL011W)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GRR1(YJR090C)|FD-Score:5.87|P-value:2.16E-9||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSY2(YLR258W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:IMG2(YCR071C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:INP52(YNL106C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain Gene:KDX1(YKL161C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LRS4(YDR439W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MDS3(YGL197W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIP6(YHR015W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:OSH3(YHR073W)|FD-Score:4.27|P-value:9.91E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAN5(YHR063C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PBP2(YBR233W)|FD-Score:5.64|P-value:8.69E-9||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO8(YDR481C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PRO1(YDR300C)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PST2(YDR032C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTK2(YJR059W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD14(YMR201C)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RKM5(YLR137W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RPL29(YFR032C-A)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS4B(YHR203C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SCD6(YPR129W)|FD-Score:6.04|P-value:7.74E-10||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SHM2(YLR058C)|FD-Score:5.44|P-value:2.64E-8||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SMI1(YGR229C)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPS2(YDR522C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRC1(YML034W)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SWM2(YNR004W_p)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAT1(YBR069C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TUB3(YML124C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URH1(YDR400W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YAR1(YPL239W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YDR008C(YDR008C_d)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGL034C(YGL034C_d)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR126W(YGR126W_p)|FD-Score:4.36|P-value:6.50E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR291C(YGR291C_d)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR059C(YOR059C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YPL191C(YPL191C_p)|FD-Score:4|P-value:3.12E-5||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YTA7(YGR270W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:AIM36(YMR157C)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALE1(YOR175C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APA1(YCL050C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:ATG15(YCR068W)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BAP2(YBR068C)|FD-Score:4.29|P-value:8.87E-6||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BEM4(YPL161C)|FD-Score:6.42|P-value:6.89E-11||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA5(YLR231C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BOI2(YER114C)|FD-Score:4.88|P-value:5.38E-7||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BUD21(YOR078W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CIR2(YOR356W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CTF19(YPL018W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CUP2(YGL166W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:CWH43(YCR017C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DSE1(YER124C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSK2(YMR276W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:DUN1(YDL101C)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ERG2(YMR202W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FYV5(YCL058C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAL11(YOL051W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GIR2(YDR152W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLC3(YEL011W)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GRR1(YJR090C)|FD-Score:5.87|P-value:2.16E-9||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSY2(YLR258W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:IMG2(YCR071C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:INP52(YNL106C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain Gene:KDX1(YKL161C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LRS4(YDR439W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MDS3(YGL197W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIP6(YHR015W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:OSH3(YHR073W)|FD-Score:4.27|P-value:9.91E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAN5(YHR063C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PBP2(YBR233W)|FD-Score:5.64|P-value:8.69E-9||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO8(YDR481C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PRO1(YDR300C)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PST2(YDR032C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTK2(YJR059W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD14(YMR201C)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RKM5(YLR137W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RPL29(YFR032C-A)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS4B(YHR203C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SCD6(YPR129W)|FD-Score:6.04|P-value:7.74E-10||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SHM2(YLR058C)|FD-Score:5.44|P-value:2.64E-8||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SMI1(YGR229C)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPS2(YDR522C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRC1(YML034W)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SWM2(YNR004W_p)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAT1(YBR069C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TUB3(YML124C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URH1(YDR400W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YAR1(YPL239W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YDR008C(YDR008C_d)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGL034C(YGL034C_d)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR126W(YGR126W_p)|FD-Score:4.36|P-value:6.50E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR291C(YGR291C_d)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR059C(YOR059C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YPL191C(YPL191C_p)|FD-Score:4|P-value:3.12E-5||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YTA7(YGR270W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL247W4.865.82E-70.58YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YHR190W4.356.85E-60.58ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YPR048W4.308.48E-60.58TAH18Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
YDR236C4.003.21E-50.58FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YFL005W3.944.11E-50.58SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YMR033W3.739.39E-50.58ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YPL122C3.711.05E-40.58TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YER133W3.691.11E-40.58GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YBR140C3.691.12E-40.58IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YHR107C3.661.27E-40.58CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YHR072W3.591.67E-40.58ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YOR257W3.482.52E-40.58CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YKL172W3.472.60E-40.58EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YKL111C_d3.452.81E-40.58YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YBL040C2.870.002050.12ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL161C6.426.89E-11BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YPR129W6.047.74E-10SCD6Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
YJR090C5.872.16E-9GRR1F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification
YBR233W5.648.69E-9PBP2RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress
YLR058C5.442.64E-8SHM2Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
YER114C4.885.38E-7BOI2Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
YCR068W4.651.62E-6ATG15Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
YGR126W_p4.366.50E-6YGR126W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YBR068C4.298.87E-6BAP2High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
YDL101C4.279.69E-6DUN1Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
YHR073W4.279.91E-6OSH3Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YOR078W4.241.12E-5BUD21Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
YMR202W4.241.14E-5ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YLR137W4.161.56E-5RKM5Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
YPL191C_p4.003.12E-5YPL191C_pPutative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

GO enrichment analysis for SGTC_1467
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0953.03E-13SGTC_12370330-0133 46.1 μMChemDiv (Drug-like library)X12370.0597015ergosterol biosynthesis
0.0921.20E-12SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.101449iron homeostasis
0.0831.84E-10SGTC_33439148379 19.0 μMChembridge (Drug-like library)19550360.0897436
0.0814.12E-10SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0779221ERG2
0.0791.06E-9SGTC_5554296-0685 37.0 μMChemDiv (Drug-like library)29085400.164384ergosterol biosynthesis
0.0763.96E-9SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.0853659ERG2
0.0764.64E-9SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0606061ERG2
0.0741.39E-8SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.09375ergosterol biosynthesis
0.0723.46E-8SGTC_31729105863 49.5 μMChembridge (Drug-like library)23487850.0759494ERG2
0.0715.09E-8SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0555556ERG2
0.0706.84E-8SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0821918ERG2
0.0691.37E-7SGTC_30989119039 49.5 μMChembridge (Drug-like library)171615280.0821918Golgi
0.0672.64E-7SGTC_6090986-0026 86.3 μMChemDiv (Drug-like library)28486100.0735294calcium & mitochondrial duress
0.0663.37E-7SGTC_13481493-0373 93.8 μMChemDiv (Drug-like library)30101290.0833333
0.0654.77E-7SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.0645161calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1037k284-109178.7 μM0.5957453334711ChemDiv (Drug-like library)285.3392833.98522
SGTC_1505k297-003720.6 μM0.5957454089396ChemDiv (Drug-like library)301.793884.44421
SGTC_282k297-003665.7 μM0.565061378ChemDiv (Drug-like library)301.793884.44421
SGTC_1468k284-1086134 μM0.46428615996457ChemDiv (Drug-like library)360.453842.80634DNA intercalators
SGTC_1504k297-003330 μM0.44388117ChemDiv (Drug-like library)413.966764.69123
SGTC_14083909-797648.8 μM0.29687524152374ChemDiv (Drug-like library)315.456284.12215Golgi
SGTC_2927901180247.9 μM0.26666743912568Chembridge (Drug-like library)308.19363.0512
SGTC_2834900175558.44 μM0.2461542989575Chembridge (Drug-like library)376.289163.28215
SGTC_20375169046200 μM0.2456145718022Chembridge (Fragment library)204.22518-0.0923460S ribosome export
SGTC_22127123476200 μM0.2452831085715Chembridge (Fragment library)204.291382.85711