4466-0538

6-(5-phenyl-1,2-dihydropyrazol-3-ylidene)cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_147
Screen concentration 29.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 6742687
SMILES C1=CC=C(C=C1)C2=CC(=C3C=CC=CC3=O)NN2
Standardized SMILES Oc1ccccc1c2cc([nH]n2)c3ccccc3
Molecular weight 236.2686
ALogP 1.35
H-bond donor count 2
H-bond acceptor count 3
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.79
% growth inhibition (Hom. pool) 19.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6742687
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:4.49|P-value:3.52E-6|Clearance:0.05||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCT2(YIL142W)|FD-Score:3.1|P-value:9.68E-4|Clearance:0.07||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC19(YAL038W)|FD-Score:4.34|P-value:6.97E-6|Clearance:0.18||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CIA1(YDR267C)|FD-Score:3.32|P-value:4.51E-4|Clearance:0.13||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:CKS1(YBR135W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.03||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:DAM1(YGR113W)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.09||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:ERG10(YPL028W)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.01||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:ERG11(YHR007C)|FD-Score:4.17|P-value:1.52E-5|Clearance:0.22||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:-4.74|P-value:1.06E-6|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG7(YHR072W)|FD-Score:16.6|P-value:5.05E-62|Clearance:11.97||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GCD2(YGR083C)|FD-Score:-3.59|P-value:1.65E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:IMP3(YHR148W)|FD-Score:-3.76|P-value:8.59E-5|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:IQG1(YPL242C)|FD-Score:3.41|P-value:3.31E-4|Clearance:0.09||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAP95(YLR347C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.05||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MCM5(YLR274W)|FD-Score:4.44|P-value:4.49E-6|Clearance:0.1||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MTW1(YAL034W-A)|FD-Score:4.58|P-value:2.34E-6|Clearance:0.09||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:PIK1(YNL267W)|FD-Score:3.19|P-value:7.02E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:POP4(YBR257W)|FD-Score:4.6|P-value:2.08E-6|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP8(YBL018C)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRI1(YIR008C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RIX1(YHR197W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.03||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RRP15(YPR143W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.07||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:SEC20(YDR498C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.08||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SWI1(YPL016W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.12||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF12(YDR145W)|FD-Score:3.83|P-value:6.39E-5|Clearance:0.01||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF13(YML098W)|FD-Score:3.76|P-value:8.62E-5|Clearance:0.03||SGD DESC:TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors Gene:TEL2(YGR099W)|FD-Score:3.72|P-value:9.90E-5|Clearance:0.04||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:YPR177C(YPR177C_d)|FD-Score:3.19|P-value:7.05E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:ARC19(YKL013C)|FD-Score:4.49|P-value:3.52E-6|Clearance:0.05||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCT2(YIL142W)|FD-Score:3.1|P-value:9.68E-4|Clearance:0.07||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC19(YAL038W)|FD-Score:4.34|P-value:6.97E-6|Clearance:0.18||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CIA1(YDR267C)|FD-Score:3.32|P-value:4.51E-4|Clearance:0.13||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:CKS1(YBR135W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.03||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:DAM1(YGR113W)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.09||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:ERG10(YPL028W)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.01||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:ERG11(YHR007C)|FD-Score:4.17|P-value:1.52E-5|Clearance:0.22||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:-4.74|P-value:1.06E-6|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG7(YHR072W)|FD-Score:16.6|P-value:5.05E-62|Clearance:11.97||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GCD2(YGR083C)|FD-Score:-3.59|P-value:1.65E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:IMP3(YHR148W)|FD-Score:-3.76|P-value:8.59E-5|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:IQG1(YPL242C)|FD-Score:3.41|P-value:3.31E-4|Clearance:0.09||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAP95(YLR347C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.05||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MCM5(YLR274W)|FD-Score:4.44|P-value:4.49E-6|Clearance:0.1||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MTW1(YAL034W-A)|FD-Score:4.58|P-value:2.34E-6|Clearance:0.09||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:PIK1(YNL267W)|FD-Score:3.19|P-value:7.02E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:POP4(YBR257W)|FD-Score:4.6|P-value:2.08E-6|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP8(YBL018C)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRI1(YIR008C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RIX1(YHR197W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.03||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RRP15(YPR143W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.07||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:SEC20(YDR498C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.08||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SWI1(YPL016W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.12||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF12(YDR145W)|FD-Score:3.83|P-value:6.39E-5|Clearance:0.01||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF13(YML098W)|FD-Score:3.76|P-value:8.62E-5|Clearance:0.03||SGD DESC:TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors Gene:TEL2(YGR099W)|FD-Score:3.72|P-value:9.90E-5|Clearance:0.04||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:YPR177C(YPR177C_d)|FD-Score:3.19|P-value:7.05E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6742687
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGE2(YIL044C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:APA1(YCL050C)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:APS3(YJL024C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASG7(YJL170C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP22(YDR350C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:AZR1(YGR224W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BAG7(YOR134W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BCK1(YJL095W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CIR2(YOR356W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CLC1(YGR167W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLN3(YAL040C)|FD-Score:-5.5|P-value:1.94E-8||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CPR5(YDR304C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CSG2(YBR036C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSR2(YPR030W)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CWH41(YGL027C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DSS1(YMR287C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DSS4(YPR017C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECI1(YLR284C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EHT1(YBR177C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:EMC2(YJR088C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:EOS1(YNL080C)|FD-Score:-3.82|P-value:6.80E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FAA4(YMR246W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:GAL10(YBR019C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GSF2(YML048W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GYP6(YJL044C)|FD-Score:-3.78|P-value:7.89E-5||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HMI1(YOL095C)|FD-Score:4.36|P-value:6.40E-6||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HVG1(YER039C)|FD-Score:6.04|P-value:7.60E-10||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:ICS3(YJL077C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:KRE6(YPR159W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LOA1(YPR139C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MBP1(YDL056W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MFT1(YML062C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MGT1(YDL200C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MKT1(YNL085W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MOH1(YBL049W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC54(YOR177C)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRP1(YDR347W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPL38(YKL170W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MSB4(YOL112W)|FD-Score:-4.4|P-value:5.41E-6||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSD1(YPL104W)|FD-Score:4.78|P-value:8.56E-7||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSI1(YBR195C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSO1(YNR049C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NAB6(YML117W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NAS2(YIL007C)|FD-Score:6.22|P-value:2.50E-10||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:OSM1(YJR051W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAH1(YMR165C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PAR32(YDL173W)|FD-Score:5.34|P-value:4.56E-8||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDE1(YGL248W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PDX3(YBR035C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PHA2(YNL316C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PMT2(YAL023C)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PPR1(YLR014C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PRM10(YJL108C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PSY2(YNL201C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PTK2(YJR059W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUS4(YNL292W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:QRI5(YLR204W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RFX1(YLR176C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGC1(YPR115W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RHR2(YIL053W)|FD-Score:-3.73|P-value:9.60E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:7.02|P-value:1.12E-12||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-5.25|P-value:7.52E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14B(YJL191W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:-5.44|P-value:2.63E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTK1(YDL025C)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SGF29(YCL010C)|FD-Score:4.76|P-value:9.46E-7||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SLG1(YOR008C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SRO9(YCL037C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYC1(YOR179C)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:9.26|P-value:1.07E-20||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM732(YMR259C_p)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UMP1(YBR173C)|FD-Score:-3.87|P-value:5.39E-5||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:UPS3(YDR185C)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:UTR1(YJR049C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS65(YLR322W_d)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR013C(YBR013C_p)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER077C(YER077C_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER175W-A(YER175W-A_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function Gene:YIG1(YPL201C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL215C(YJL215C_d)|FD-Score:-5.16|P-value:1.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKE4(YIL023C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLF2(YHL014C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR030W(YLR030W_p)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Putative protein of unknown function Gene:YMR194C-A(YMR194C-A_d)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YPL150W(YPL150W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPL199C(YPL199C_p)|FD-Score:-3.89|P-value:5.11E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:AFT1(YGL071W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGE2(YIL044C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:APA1(YCL050C)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:APS3(YJL024C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASG7(YJL170C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP22(YDR350C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:AZR1(YGR224W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BAG7(YOR134W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BCK1(YJL095W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CIR2(YOR356W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CLC1(YGR167W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLN3(YAL040C)|FD-Score:-5.5|P-value:1.94E-8||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CPR5(YDR304C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CSG2(YBR036C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSR2(YPR030W)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CWH41(YGL027C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DSS1(YMR287C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DSS4(YPR017C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECI1(YLR284C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EHT1(YBR177C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:EMC2(YJR088C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:EOS1(YNL080C)|FD-Score:-3.82|P-value:6.80E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FAA4(YMR246W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:GAL10(YBR019C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GSF2(YML048W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GYP6(YJL044C)|FD-Score:-3.78|P-value:7.89E-5||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HMI1(YOL095C)|FD-Score:4.36|P-value:6.40E-6||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HVG1(YER039C)|FD-Score:6.04|P-value:7.60E-10||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:ICS3(YJL077C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:KRE6(YPR159W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LOA1(YPR139C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MBP1(YDL056W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MFT1(YML062C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MGT1(YDL200C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MKT1(YNL085W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MOH1(YBL049W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC54(YOR177C)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRP1(YDR347W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPL38(YKL170W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MSB4(YOL112W)|FD-Score:-4.4|P-value:5.41E-6||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSD1(YPL104W)|FD-Score:4.78|P-value:8.56E-7||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSI1(YBR195C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSO1(YNR049C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NAB6(YML117W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NAS2(YIL007C)|FD-Score:6.22|P-value:2.50E-10||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:OSM1(YJR051W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAH1(YMR165C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PAR32(YDL173W)|FD-Score:5.34|P-value:4.56E-8||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDE1(YGL248W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PDX3(YBR035C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PHA2(YNL316C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PMT2(YAL023C)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PPR1(YLR014C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PRM10(YJL108C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PSY2(YNL201C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PTK2(YJR059W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUS4(YNL292W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:QRI5(YLR204W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RFX1(YLR176C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGC1(YPR115W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RHR2(YIL053W)|FD-Score:-3.73|P-value:9.60E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:7.02|P-value:1.12E-12||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-5.25|P-value:7.52E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14B(YJL191W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:-5.44|P-value:2.63E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTK1(YDL025C)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SGF29(YCL010C)|FD-Score:4.76|P-value:9.46E-7||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SLG1(YOR008C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SRO9(YCL037C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYC1(YOR179C)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:9.26|P-value:1.07E-20||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM732(YMR259C_p)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UMP1(YBR173C)|FD-Score:-3.87|P-value:5.39E-5||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:UPS3(YDR185C)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:UTR1(YJR049C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS65(YLR322W_d)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR013C(YBR013C_p)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER077C(YER077C_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER175W-A(YER175W-A_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function Gene:YIG1(YPL201C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL215C(YJL215C_d)|FD-Score:-5.16|P-value:1.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKE4(YIL023C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLF2(YHL014C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR030W(YLR030W_p)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Putative protein of unknown function Gene:YMR194C-A(YMR194C-A_d)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YPL150W(YPL150W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPL199C(YPL199C_p)|FD-Score:-3.89|P-value:5.11E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR072W16.605.05E-6212.00ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YBR257W4.602.08E-60.03POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YAL034W-A4.582.34E-60.09MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YKL013C4.493.52E-60.05ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YLR274W4.444.49E-60.10MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YAL038W4.346.97E-60.17CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YHR007C4.171.52E-50.22ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YPL016W3.953.91E-50.12SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YDR145W3.836.39E-50.01TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YBL018C3.826.62E-50.07POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YML098W3.768.62E-50.03TAF13TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
YGR099W3.729.90E-50.04TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YPR143W3.681.15E-40.07RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YGR113W3.611.52E-40.09DAM1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
YLR347C3.532.11E-40.05KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR069C9.261.07E-20TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YLR406C7.021.12E-12RPL31BRibosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
YIL007C6.222.50E-10NAS2Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress
YER039C6.047.60E-10HVG1Protein of unknown function, has homology to Vrg4p
YJL108C5.561.35E-8PRM10Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation
YDL173W5.344.56E-8PAR32Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene
YPL104W4.788.56E-7MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YCL010C4.769.46E-7SGF29Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
YOL095C4.366.40E-6HMI1Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
YGL027C4.201.35E-5CWH41Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
YBL049W4.191.41E-5MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YPR017C4.151.65E-5DSS4Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
YMR264W4.151.66E-5CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YJL024C4.151.69E-5APS3Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
YDL056W4.131.78E-5MBP1Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes

GO enrichment analysis for SGTC_147
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2143.82E-62SGTC_5554296-0685 37.0 μMChemDiv (Drug-like library)29085400.0555556ergosterol biosynthesis
0.2011.08E-54SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.101695ergosterol biosynthesis
0.1641.05E-36SGTC_12370330-0133 46.1 μMChemDiv (Drug-like library)X12370.0689655ergosterol biosynthesis
0.1553.96E-33SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.109091ergosterol biosynthesis
0.1547.56E-33SGTC_1547harmine 94.2 μMMiscellaneous52809530.107143ergosterol biosynthesis
0.1482.86E-30SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.0655738ergosterol biosynthesis
0.1402.85E-27SGTC_269exalamide 29.0 μMMiscellaneous33160.0892857ergosterol biosynthesis
0.1323.17E-24SGTC_2525harmine 47.9 μMMiscellaneous52809530.107143ergosterol biosynthesis
0.1133.94E-18SGTC_610978-8327 98.6 μMChemDiv (Drug-like library)61500590.101695ergosterol biosynthesis
0.1111.19E-17SGTC_7342023-0417 80.0 μMChemDiv (Drug-like library)39357320.0615385ergosterol biosynthesis
0.1041.49E-15SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.0727273azole & statin
0.0977.16E-14SGTC_29749094440 9.7 μMChembridge (Drug-like library)252361340.0571429ergosterol biosynthesis
0.0971.01E-13SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.0517241ergosterol biosynthesis
0.0952.50E-13SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.0727273ergosterol biosynthesis
0.0881.02E-11SGTC_14324049-0298 68.4 μMChemDiv (Drug-like library)7416460.0677966

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3333486-013140.1 μM0.6341466744690ChemDiv (Drug-like library)296.320541.31525amide catabolism
SGTC_11443486-018312.5 μM0.523816744069ChemDiv (Drug-like library)296.320541.0225
SGTC_13953486-01904.35 μM0.426748079ChemDiv (Drug-like library)316.353242.38224
SGTC_8490386-0493241 μM0.292683726119ChemDiv (Drug-like library)235.283823.28422
SGTC_2181631624054.26 μM0.29268312779990Chembridge (Fragment library)200.233182.50122
SGTC_1830st05598250.3 μM0.266667455313TimTec (Natural product derivative library)254.23752.35624
SGTC_2507purpurin79.67 μM0.2619056683Microsource (Natural product library)256.210322.08235copper-dependent oxidative stress
SGTC_15130337-055919 μM0.26087775073ChemDiv (Drug-like library)211.216063.03813
SGTC_2671danthron100 μM0.256412950Microsource (Natural product library)240.210922.32424DNA damage response
SGTC_20084012253172 μM0.255814603940Chembridge (Fragment library)185.225142.05513