k295-0292

3-[2-oxo-2-(4-phenyl-3,6-dihydro-2H-pyridin-1-yl)ethyl]-1,2,3-benzotriazin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1470
Screen concentration 231.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5045612
SMILES C1CN(CC=C1C2=CC=CC=C2)C(=O)CN3C(=O)C4=CC=CC=C4N=N3
Standardized SMILES O=C(CN1N=Nc2ccccc2C1=O)N3CCC(=CC3)c4ccccc4
Molecular weight 346.3825
ALogP 3.18
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.52
% growth inhibition (Hom. pool) 6.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5045612
Download HIP data (tab-delimited text)  (excel)
Gene:NDC1(YML031W)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.21||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NSE1(YLR007W)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.11||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:OST2(YOR103C)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.23||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PIS1(YPR113W)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.12||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PZF1(YPR186C)|FD-Score:4|P-value:3.19E-5|Clearance:0.02||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RPC31(YNL151C)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.14||SGD DESC:RNA polymerase III subunit C31 Gene:RPL15A(YLR029C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:SEC4(YFL005W)|FD-Score:3.74|P-value:9.09E-5|Clearance:0.21||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:TUB1(YML085C)|FD-Score:4.75|P-value:1.04E-6|Clearance:0.64||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTR5(YEL035C_p)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.04||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:NDC1(YML031W)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.21||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NSE1(YLR007W)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.11||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:OST2(YOR103C)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.23||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PIS1(YPR113W)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.12||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PZF1(YPR186C)|FD-Score:4|P-value:3.19E-5|Clearance:0.02||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RPC31(YNL151C)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.14||SGD DESC:RNA polymerase III subunit C31 Gene:RPL15A(YLR029C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:SEC4(YFL005W)|FD-Score:3.74|P-value:9.09E-5|Clearance:0.21||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:TUB1(YML085C)|FD-Score:4.75|P-value:1.04E-6|Clearance:0.64||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTR5(YEL035C_p)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.04||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5045612
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AFT1(YGL071W)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO1(YDR127W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP5(YNL059C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:AVT1(YJR001W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BAP2(YBR068C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:CCW12(YLR110C)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUS2(YNL286W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DST1(YGL043W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ERI1(YPL096C-A)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FSH3(YOR280C)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FUI1(YBL042C)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAL1(YBR020W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCN2(YDR283C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GSF2(YML048W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GSY2(YLR258W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:IOC4(YMR044W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LNP1(YHR192W_p)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MCA1(YOR197W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MRPL31(YKL138C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OLA1(YBR025C)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:ROM2(YLR371W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:8.19|P-value:1.35E-16||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS19A(YOL121C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SNA3(YJL151C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SPO12(YHR152W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:TDA5(YLR426W_p)|FD-Score:6.67|P-value:1.32E-11||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TMA23(YMR269W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:UBX2(YML013W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VPS61(YDR136C_d)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:7.11|P-value:5.62E-13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK1(YHR135C)|FD-Score:3.74|P-value:9.05E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDR124W(YDR124W_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR220C(YDR220C_d)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIP4(YGL198W)|FD-Score:4.32|P-value:7.91E-6||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR096W(YJR096W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKL118W(YKL118W_d)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR036C(YLR036C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Putative protein of unknown function Gene:YLR446W(YLR446W_p)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML012C-A(YML012C-A_d)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YOR093C(YOR093C_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YPR059C(YPR059C_d)|FD-Score:7.82|P-value:2.73E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YTP1(YNL237W)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:YVH1(YIR026C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:AAD3(YCR107W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AFT1(YGL071W)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO1(YDR127W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP5(YNL059C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:AVT1(YJR001W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BAP2(YBR068C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:CCW12(YLR110C)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUS2(YNL286W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DST1(YGL043W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ERI1(YPL096C-A)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FSH3(YOR280C)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FUI1(YBL042C)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAL1(YBR020W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCN2(YDR283C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GSF2(YML048W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GSY2(YLR258W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:IOC4(YMR044W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LNP1(YHR192W_p)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MCA1(YOR197W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MRPL31(YKL138C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OLA1(YBR025C)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:ROM2(YLR371W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:8.19|P-value:1.35E-16||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS19A(YOL121C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SNA3(YJL151C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SPO12(YHR152W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:TDA5(YLR426W_p)|FD-Score:6.67|P-value:1.32E-11||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TMA23(YMR269W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:UBX2(YML013W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VPS61(YDR136C_d)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:7.11|P-value:5.62E-13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK1(YHR135C)|FD-Score:3.74|P-value:9.05E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDR124W(YDR124W_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR220C(YDR220C_d)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIP4(YGL198W)|FD-Score:4.32|P-value:7.91E-6||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR096W(YJR096W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKL118W(YKL118W_d)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR036C(YLR036C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Putative protein of unknown function Gene:YLR446W(YLR446W_p)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML012C-A(YML012C-A_d)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YOR093C(YOR093C_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YPR059C(YPR059C_d)|FD-Score:7.82|P-value:2.73E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YTP1(YNL237W)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:YVH1(YIR026C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML085C4.751.04E-60.64TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YLR007W4.112.00E-50.11NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YPR186C4.003.19E-50.02PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YOR103C3.973.53E-50.23OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YFL005W3.749.09E-50.21SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YML031W3.542.03E-40.21NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YPR113W3.324.44E-40.12PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YEL035C_p3.216.72E-40.04UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YLR029C3.177.69E-40.06RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YNL151C3.119.45E-40.14RPC31RNA polymerase III subunit C31
YDR166C2.970.001500.10SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YGL169W2.870.002050.01SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YGR013W2.860.002110.03SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
YCR057C2.830.002300.13PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YNL178W2.700.003420.10RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL020C8.191.35E-16RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPR059C_d7.822.73E-15YPR059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
YLR261C_d7.115.62E-13VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YLR426W_p6.671.32E-11TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YGL071W5.601.05E-8AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YKL118W_d5.591.13E-8YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YML012C-A_d4.444.42E-6YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YBL042C4.444.46E-6FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YGL198W4.327.91E-6YIP4Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
YMR269W4.072.37E-5TMA23Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
YHR152W4.022.96E-5SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
YLR446W_p3.934.22E-5YLR446W_pPutative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene
YGL043W3.885.22E-5DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YOR280C3.875.50E-5FSH3Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
YBR025C3.807.34E-5OLA1P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress

GO enrichment analysis for SGTC_1470
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1722.81E-40SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.117647
0.1694.01E-39SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0416667
0.1505.42E-31SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.0930233
0.1461.13E-29SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.117647
0.1398.19E-27SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0615385
0.1373.50E-26SGTC_28519015922 71.4 μMChembridge (Drug-like library)29968930.101266
0.1374.51E-26SGTC_14884013-0813 75.9 μMChemDiv (Drug-like library)29100570.153846RPP1 & pyrimidine depletion
0.1292.54E-23SGTC_2695st077686 52.0 μMTimTec (Natural product derivative library)28760920.133333
0.1284.34E-23SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.0864198
0.1254.06E-22SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.0810811
0.1254.79E-22SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0724638
0.1241.13E-21SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.128205
0.1232.48E-21SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.103448PDR1
0.1226.33E-21SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.168831
0.1181.10E-19SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.1125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2832799276251.95 μM0.4655172985177Chembridge (Drug-like library)299.71183.75204
SGTC_21775811945192 μM0.2698415343475Chembridge (Fragment library)201.224540.91212
SGTC_21785812956200 μM0.2656255403383Chembridge (Fragment library)215.251121.49212
SGTC_3393091-483371.4 μM0.2575763240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_13853091-483414.1 μM0.2575764072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion
SGTC_5931203-021861.7 μM0.2461543666956ChemDiv (Drug-like library)244.33213.65302
SGTC_21685727979200 μM0.245902695210Chembridge (Fragment library)206.26422.23603
SGTC_13993562-577225 μM0.2423847490ChemDiv (Drug-like library)394.7829834.86305RPP1 & pyrimidine depletion
SGTC_23829035899200 μM0.2352946462679Chembridge (Fragment library)288.368041.9431460S ribosome export
SGTC_3084911687749.47 μM0.23376627241325Chembridge (Drug-like library)385.28814.07205