k673-0312

[4-(4-bromophenyl)sulfonyl-3-nitrophenyl]-pyrrolidin-1-ylmethanone

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1471
Screen concentration 37.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 24099884
SMILES C1CCN(C1)C(=O)C2=CC(=C(C=C2)S(=O)(=O)C3=CC=C(C=C3)Br)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1cc(ccc1S(=O)(=O)c2ccc(Br)cc2)C(=O)N3CCCC3
Molecular weight 439.2804
ALogP 3.45
H-bond donor count 0
H-bond acceptor count 5
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.44
% growth inhibition (Hom. pool) 5.96


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24099884
Download HIP data (tab-delimited text)  (excel)
Gene:BFR2(YDR299W)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:EFB1(YAL003W)|FD-Score:-3.12|P-value:8.90E-4|Clearance:0||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERB1(YMR049C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.17||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:MCM7(YBR202W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.04||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:POP1(YNL221C)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RNA14(YMR061W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.05||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC53(YDL150W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0.08||SGD DESC:RNA polymerase III subunit C53 Gene:RPC82(YPR190C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.09||SGD DESC:RNA polymerase III subunit C82 Gene:RPL15A(YLR029C)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.05||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.42||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC62(YPL094C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.14||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:YDL152W(YDL152W_d)|FD-Score:4.09|P-value:2.14E-5|Clearance:0.42||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:BFR2(YDR299W)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:EFB1(YAL003W)|FD-Score:-3.12|P-value:8.90E-4|Clearance:0||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERB1(YMR049C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.17||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:MCM7(YBR202W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.04||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:POP1(YNL221C)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RNA14(YMR061W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.05||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC53(YDL150W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0.08||SGD DESC:RNA polymerase III subunit C53 Gene:RPC82(YPR190C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.09||SGD DESC:RNA polymerase III subunit C82 Gene:RPL15A(YLR029C)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.05||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.42||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC62(YPL094C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.14||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:YDL152W(YDL152W_d)|FD-Score:4.09|P-value:2.14E-5|Clearance:0.42||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24099884
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ACB1(YGR037C)|FD-Score:6.56|P-value:2.75E-11||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADA2(YDR448W)|FD-Score:6.43|P-value:6.27E-11||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ATG14(YBR128C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:AVT1(YJR001W)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAP2(YIL034C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAP2(YHR028C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DSF2(YBR007C_p)|FD-Score:5.77|P-value:3.89E-9||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUN1(YDL101C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EAF7(YNL136W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:FUR4(YBR021W)|FD-Score:12.4|P-value:1.13E-35||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLO3(YER122C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GSY2(YLR258W)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ISM1(YPL040C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LEM3(YNL323W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MLP1(YKR095W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MNT2(YGL257C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRPL32(YCR003W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NGG1(YDR176W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PEP3(YLR148W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PHO85(YPL031C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PKH3(YDR466W)|FD-Score:4.33|P-value:7.36E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PKR1(YMR123W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PSK1(YAL017W)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:RPL13B(YMR142C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:SIF2(YBR103W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SKM1(YOL113W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SPF1(YEL031W)|FD-Score:4.42|P-value:4.99E-6||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSD1(YDR293C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TSC3(YBR058C-A)|FD-Score:6.57|P-value:2.52E-11||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YBP2(YGL060W)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YGR111W(YGR111W_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJR115W(YJR115W_p)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKL077W(YKL077W_p)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLL020C(YLL020C_d)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YML100W-A(YML100W-A_p)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL120C(YNL120C_d)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:AAD3(YCR107W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ACB1(YGR037C)|FD-Score:6.56|P-value:2.75E-11||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADA2(YDR448W)|FD-Score:6.43|P-value:6.27E-11||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ATG14(YBR128C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:AVT1(YJR001W)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAP2(YIL034C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAP2(YHR028C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DSF2(YBR007C_p)|FD-Score:5.77|P-value:3.89E-9||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUN1(YDL101C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EAF7(YNL136W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:FUR4(YBR021W)|FD-Score:12.4|P-value:1.13E-35||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLO3(YER122C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GSY2(YLR258W)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ISM1(YPL040C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LEM3(YNL323W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MLP1(YKR095W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MNT2(YGL257C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRPL32(YCR003W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NGG1(YDR176W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PEP3(YLR148W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PHO85(YPL031C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PKH3(YDR466W)|FD-Score:4.33|P-value:7.36E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PKR1(YMR123W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PSK1(YAL017W)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:RPL13B(YMR142C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:SIF2(YBR103W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SKM1(YOL113W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SPF1(YEL031W)|FD-Score:4.42|P-value:4.99E-6||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSD1(YDR293C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TSC3(YBR058C-A)|FD-Score:6.57|P-value:2.52E-11||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YBP2(YGL060W)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YGR111W(YGR111W_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJR115W(YJR115W_p)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKL077W(YKL077W_p)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLL020C(YLL020C_d)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YML100W-A(YML100W-A_p)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL120C(YNL120C_d)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C4.191.38E-50.42RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL152W_d4.092.14E-50.42YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YDR280W3.671.22E-40.04RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YDL150W3.631.43E-40.08RPC53RNA polymerase III subunit C53
YMR061W3.551.95E-40.05RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YPR190C3.492.38E-40.09RPC82RNA polymerase III subunit C82
YLR029C3.413.28E-40.05RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YMR049C3.363.96E-40.17ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YBR202W3.197.11E-40.04MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YNL221C3.158.14E-40.03POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YPL094C3.129.16E-40.14SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YOR260W2.980.001440.04GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YLR274W2.940.001620.12MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YBR070C2.830.002340.03ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YER094C2.790.002610.13PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR021W12.401.13E-35FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YBR058C-A6.572.52E-11TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGR037C6.562.75E-11ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YDR448W6.436.27E-11ADA2Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
YBR007C_p5.773.89E-9DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL060W4.904.89E-7YBP2Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
YLR258W4.621.96E-6GSY2Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
YNL120C_d4.602.11E-6YNL120C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
YEL031W4.424.99E-6SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YDR466W4.337.36E-6PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
YDL101C4.131.79E-5DUN1Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
YKL077W_p3.865.74E-5YKL077W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YJR001W3.846.17E-5AVT1Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YDR293C3.807.20E-5SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YIL034C3.729.93E-5CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress

GO enrichment analysis for SGTC_1471
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2857.71E-111SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0945946RNA processing & uracil transport
0.2781.46E-105SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.0704225RNA processing & uracil transport
0.2531.29E-86SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.0704225RNA processing & uracil transport
0.2261.95E-69SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.0547945RNA processing & uracil transport
0.2243.19E-68SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.168831RNA processing & uracil transport
0.2161.56E-63SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0416667TSC3-RPN4
0.2034.89E-56SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.084507TSC3-RPN4
0.2014.60E-55SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0470588TSC3-RPN4
0.1951.64E-51SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.0632911RNA processing & uracil transport
0.1937.57E-51SGTC_22977971671 152.3 μMChembridge (Fragment library)9090160.0649351RNA processing & uracil transport
0.1871.85E-47SGTC_452diindolylmethane 8.0 μMICCB bioactive library30710.0483871RNA processing & uracil transport
0.1811.32E-44SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.0740741RNA processing & uracil transport
0.1782.39E-43SGTC_3051682-2595 20.8 μMChemDiv (Drug-like library)2260330.169231RNA processing & uracil transport
0.1763.16E-42SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0779221TSC3-RPN4
0.1722.80E-40SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_16794130-12766.7 μM0.4032261005053Miscellaneous363.7707033.63705Golgi
SGTC_2914130-127835.5 μM0.3731341068044ChemDiv (Drug-like library)375.806223.41505Golgi
SGTC_600986-0278202 μM0.317463110598ChemDiv (Drug-like library)415.056744.56703
SGTC_990109-0120622.8 μM0.315789759414ChemDiv (Drug-like library)321.126163.25713
SGTC_1340269-00186.92 μM0.3114754378915ChemDiv (Drug-like library)411.121283.04617
SGTC_12590671-013243 μM0.306452X1259ChemDiv (Drug-like library)371.206443.44641
SGTC_7930488-0545106 μM0.303571292548ChemDiv (Drug-like library)237.212042.24105
SGTC_1893970-07422.12 μM0.2982462891807ChemDiv (Drug-like library)297.06172.03104
SGTC_3158910000749.47 μM0.29230819297233Chembridge (Drug-like library)373.286844.01902
SGTC_1631327-00367.55 μM0.28358215992207ChemDiv (Drug-like library)432.117625.56615