3253-0744

N-[(E)-[(Z)-2-bromo-3-phenylprop-2-enylidene]amino]-2,5-dimethylfuran-3-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_148
Screen concentration 288.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6893655
SMILES CC1=CC(=C(O1)C)C(=O)NN=CC(=CC2=CC=CC=C2)Br
Standardized SMILES Cc1oc(C)c(c1)C(=O)NN=CC(=Cc2ccccc2)Br
Molecular weight 347.2065
ALogP 2.89
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.82
% growth inhibition (Hom. pool) 14.12


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6893655
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:-4.43|P-value:4.81E-6|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CDC43(YGL155W)|FD-Score:-3.99|P-value:3.30E-5|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DUT1(YBR252W)|FD-Score:3.83|P-value:6.49E-5|Clearance:0.54||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:DYS1(YHR068W)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.11||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:GCD1(YOR260W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-4.06|P-value:2.42E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSP10(YOR020C)|FD-Score:-4.97|P-value:3.34E-7|Clearance:0||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:KRE5(YOR336W)|FD-Score:4.94|P-value:3.85E-7|Clearance:0.01||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LSG1(YGL099W)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.01||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM5(YLR274W)|FD-Score:5.12|P-value:1.54E-7|Clearance:0.18||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MIA40(YKL195W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.28||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NOP4(YPL043W)|FD-Score:-3.27|P-value:5.35E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:OST2(YOR103C)|FD-Score:-3.95|P-value:3.96E-5|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PGA1(YNL158W)|FD-Score:-3.28|P-value:5.22E-4|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RPT3(YDR394W)|FD-Score:4.93|P-value:4.10E-7|Clearance:0.62||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.03||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RPT5(YOR117W)|FD-Score:5.83|P-value:2.85E-9|Clearance:0.71||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRN6(YBL014C)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SMD1(YGR074W)|FD-Score:3.87|P-value:5.39E-5|Clearance:0.05||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SMT3(YDR510W)|FD-Score:4.31|P-value:8.13E-6|Clearance:0.12||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SRP72(YPL210C)|FD-Score:-3.44|P-value:2.90E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TEL2(YGR099W)|FD-Score:3.18|P-value:7.27E-4|Clearance:0.12||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:ALG14(YBR070C)|FD-Score:-4.43|P-value:4.81E-6|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CDC43(YGL155W)|FD-Score:-3.99|P-value:3.30E-5|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DUT1(YBR252W)|FD-Score:3.83|P-value:6.49E-5|Clearance:0.54||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:DYS1(YHR068W)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.11||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:GCD1(YOR260W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-4.06|P-value:2.42E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSP10(YOR020C)|FD-Score:-4.97|P-value:3.34E-7|Clearance:0||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:KRE5(YOR336W)|FD-Score:4.94|P-value:3.85E-7|Clearance:0.01||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LSG1(YGL099W)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.01||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM5(YLR274W)|FD-Score:5.12|P-value:1.54E-7|Clearance:0.18||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MIA40(YKL195W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.28||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NOP4(YPL043W)|FD-Score:-3.27|P-value:5.35E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:OST2(YOR103C)|FD-Score:-3.95|P-value:3.96E-5|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PGA1(YNL158W)|FD-Score:-3.28|P-value:5.22E-4|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RPT3(YDR394W)|FD-Score:4.93|P-value:4.10E-7|Clearance:0.62||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.03||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RPT5(YOR117W)|FD-Score:5.83|P-value:2.85E-9|Clearance:0.71||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRN6(YBL014C)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SMD1(YGR074W)|FD-Score:3.87|P-value:5.39E-5|Clearance:0.05||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SMT3(YDR510W)|FD-Score:4.31|P-value:8.13E-6|Clearance:0.12||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SRP72(YPL210C)|FD-Score:-3.44|P-value:2.90E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TEL2(YGR099W)|FD-Score:3.18|P-value:7.27E-4|Clearance:0.12||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6893655
Download HOP data (tab-delimited text)  (excel)
Gene:ADI1(YMR009W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:AHC1(YOR023C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AIM7(YDR063W)|FD-Score:-4.84|P-value:6.41E-7||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ARG7(YMR062C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATF2(YGR177C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking Gene:ATG33(YLR356W)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:ATG34(YOL083W)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:AVT3(YKL146W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM4(YPL161C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA2(YJR078W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BTS1(YPL069C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC26(YFR036W)|FD-Score:8.41|P-value:2.00E-17||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CKA1(YIL035C)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG1(YGL223C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSN9(YDR179C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:DBF2(YGR092W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIT2(YDR402C)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:DSS1(YMR287C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:FKS3(YMR306W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLE2(YER107C)|FD-Score:5.42|P-value:3.01E-8||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPT2(YKR067W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HO(YDL227C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:IES3(YLR052W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:LSM1(YJL124C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LYS4(YDR234W)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MET32(YDR253C)|FD-Score:-5.12|P-value:1.54E-7||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MPD2(YOL088C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRC1(YCL061C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL38(YKL170W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSH6(YDR097C)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:NAT1(YDL040C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NNT1(YLR285W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:OPI7(YDR360W_d)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OSH3(YHR073W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAU5(YFL020C)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PEP1(YBL017C)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments Gene:PET111(YMR257C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET191(YJR034W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PFK2(YMR205C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFS1(YHR185C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIF1(YML061C)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKP1(YIL042C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p Gene:PTC6(YCR079W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:PTP1(YDL230W)|FD-Score:-4.47|P-value:3.97E-6||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:QRI5(YLR204W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RKM4(YDR257C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RLM1(YPL089C)|FD-Score:-3.88|P-value:5.12E-5||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPA12(YJR063W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL22A(YLR061W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:9.61|P-value:3.68E-22||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRF1(YHR038W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RSC1(YGR056W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM24(YDR175C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC1(YOL138C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SAM37(YMR060C)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAM4(YPL273W)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SNX4(YJL036W)|FD-Score:5.34|P-value:4.67E-8||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOD1(YJR104C)|FD-Score:8.14|P-value:2.00E-16||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SSB2(YNL209W)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication Gene:STB3(YDR169C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress Gene:TFB6(YOR352W)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMN2(YDR107C)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TOM70(YNL121C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRS85(YDR108W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSC3(YBR058C-A)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UPS2(YLR168C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:UTP30(YKR060W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS63(YLR261C_d)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.73|P-value:9.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL086W(YDL086W_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene Gene:YDL176W(YDL176W)|FD-Score:-4.79|P-value:8.34E-7||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR109C(YDR109C_p)|FD-Score:-5.86|P-value:2.36E-9||SGD DESC:Putative kinase Gene:YGL199C(YGL199C_d)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YIL060W(YIL060W_p)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJR037W(YJR037W_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKE4(YIL023C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL169C(YKL169C_d)|FD-Score:5.72|P-value:5.41E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR122C(YLR122C_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL200C(YNL200C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR309C(YOR309C_d)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR117W(YPR117W_p)|FD-Score:-3.92|P-value:4.49E-5||SGD DESC:Putative protein of unknown function Gene:YPS7(YDR349C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YRF1-6(YNL339C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:ZIP2(YGL249W)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADI1(YMR009W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:AHC1(YOR023C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AIM7(YDR063W)|FD-Score:-4.84|P-value:6.41E-7||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ARG7(YMR062C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATF2(YGR177C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking Gene:ATG33(YLR356W)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:ATG34(YOL083W)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:AVT3(YKL146W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM4(YPL161C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA2(YJR078W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BTS1(YPL069C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC26(YFR036W)|FD-Score:8.41|P-value:2.00E-17||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CKA1(YIL035C)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG1(YGL223C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSN9(YDR179C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:DBF2(YGR092W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIT2(YDR402C)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:DSS1(YMR287C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:FKS3(YMR306W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLE2(YER107C)|FD-Score:5.42|P-value:3.01E-8||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPT2(YKR067W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HO(YDL227C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:IES3(YLR052W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:LSM1(YJL124C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LYS4(YDR234W)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MET32(YDR253C)|FD-Score:-5.12|P-value:1.54E-7||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MPD2(YOL088C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRC1(YCL061C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL38(YKL170W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSH6(YDR097C)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:NAT1(YDL040C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NNT1(YLR285W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:OPI7(YDR360W_d)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OSH3(YHR073W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAU5(YFL020C)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PEP1(YBL017C)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments Gene:PET111(YMR257C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET191(YJR034W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PFK2(YMR205C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFS1(YHR185C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIF1(YML061C)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKP1(YIL042C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p Gene:PTC6(YCR079W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:PTP1(YDL230W)|FD-Score:-4.47|P-value:3.97E-6||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:QRI5(YLR204W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RKM4(YDR257C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RLM1(YPL089C)|FD-Score:-3.88|P-value:5.12E-5||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPA12(YJR063W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL22A(YLR061W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:9.61|P-value:3.68E-22||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRF1(YHR038W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RSC1(YGR056W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM24(YDR175C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC1(YOL138C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SAM37(YMR060C)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAM4(YPL273W)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SNX4(YJL036W)|FD-Score:5.34|P-value:4.67E-8||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOD1(YJR104C)|FD-Score:8.14|P-value:2.00E-16||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SSB2(YNL209W)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication Gene:STB3(YDR169C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress Gene:TFB6(YOR352W)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMN2(YDR107C)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TOM70(YNL121C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRS85(YDR108W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSC3(YBR058C-A)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UPS2(YLR168C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:UTP30(YKR060W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS63(YLR261C_d)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.73|P-value:9.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL086W(YDL086W_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene Gene:YDL176W(YDL176W)|FD-Score:-4.79|P-value:8.34E-7||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR109C(YDR109C_p)|FD-Score:-5.86|P-value:2.36E-9||SGD DESC:Putative kinase Gene:YGL199C(YGL199C_d)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YIL060W(YIL060W_p)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJR037W(YJR037W_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKE4(YIL023C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL169C(YKL169C_d)|FD-Score:5.72|P-value:5.41E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR122C(YLR122C_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL200C(YNL200C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR309C(YOR309C_d)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR117W(YPR117W_p)|FD-Score:-3.92|P-value:4.49E-5||SGD DESC:Putative protein of unknown function Gene:YPS7(YDR349C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YRF1-6(YNL339C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:ZIP2(YGL249W)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR117W5.832.85E-90.71RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YLR274W5.121.54E-70.18MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YOR336W4.943.85E-70.01KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YDR394W4.934.10E-70.62RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YDR510W4.318.13E-60.12SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YOR259C4.191.37E-50.03RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YKL195W4.161.58E-50.28MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YGL099W3.895.11E-50.01LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGR074W3.875.39E-50.05SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YBR252W3.836.49E-50.54DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YHR068W3.295.00E-40.11DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YGR099W3.187.27E-40.12TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YPR187W3.060.001100.00RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YHR143W-A3.060.001110.01RPC10RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
YOL120C3.050.001150.02RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL020C9.613.68E-22RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YFR036W8.412.00E-17CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YJR104C8.142.00E-16SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YKL169C_d5.725.41E-9YKL169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
YBR058C-A5.501.88E-8TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YER107C5.423.01E-8GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YJL036W5.344.67E-8SNX4Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
YIL035C5.151.33E-7CKA1Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
YMR060C4.943.83E-7SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YIL060W_p4.464.07E-6YIL060W_pPutative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
YNL200C4.261.01E-5YNL200CNADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOR309C_d4.181.43E-5YOR309C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
YLR261C_d4.082.28E-5VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YNL298W4.082.30E-5CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YJL124C3.983.40E-5LSM1Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress

GO enrichment analysis for SGTC_148
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1904.86E-49SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.183333
0.1721.91E-40SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.166667
0.1652.15E-37SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.183333
0.1625.24E-36SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.153846TSC3-RPN4
0.1611.19E-35SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.141026
0.1528.79E-32SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0952381
0.1512.16E-31SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.171875TSC3-RPN4
0.1499.96E-31SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.126984TSC3-RPN4
0.1471.08E-29SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.122807
0.1404.67E-27SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0606061
0.1351.48E-25SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.114286TSC3-RPN4
0.1351.85E-25SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.171053
0.1352.81E-25SGTC_28pentachloropyridine N-oxide 1.6 μMChemDiv (Drug-like library)1402500.0185185TSC3-RPN4
0.1331.06E-24SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0735294TSC3-RPN4
0.1331.14E-24SGTC_8790831-1030 162.0 μMChemDiv (Drug-like library)15492520.116667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8273253-0474693 μM0.5090915402065ChemDiv (Drug-like library)288.728922.66113
SGTC_22757947463200 μM0.3928571225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_7051124-0212310 μM0.3759554485ChemDiv (Drug-like library)293.1484.01212
SGTC_3008907891271.43 μM0.3516648731Chembridge (Drug-like library)287.310482.21314
SGTC_3298911685871.43 μM0.352506942Chembridge (Drug-like library)279.718862.65713
SGTC_21945927483104.55 μM0.314815760979Chembridge (Fragment library)201.221241.89112
SGTC_12011889-337245.9 μM0.3015875392199ChemDiv (Drug-like library)282.337023.49113Golgi
SGTC_6541124-027180.9 μM0.2968755403748ChemDiv (Drug-like library)309.115462.58524
SGTC_7531322-0200289 μM0.2923085403907ChemDiv (Drug-like library)275.216961.85526
SGTC_563tetrahydrosecaminediol614 μM0.283582334274ChemDiv (Drug-like library)624.898248.24734amide catabolism