3909-9022

ethyl 4-(diethylamino)-5-methylthieno[2,3-d]pyrimidine-6-carboxylate

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1485
Screen concentration 254.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 714516
SMILES CCN(CC)C1=C2C(=C(SC2=NC=N1)C(=O)OCC)C
Standardized SMILES CCOC(=O)c1sc2ncnc(N(CC)CC)c2c1C
Molecular weight 293.3846
ALogP 3.49
H-bond donor count 0
H-bond acceptor count 6
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.85
% growth inhibition (Hom. pool) 8.75


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 714516
Download HIP data (tab-delimited text)  (excel)
Gene:RPP1(YHR062C)|FD-Score:6.15|P-value:3.93E-10|Clearance:2.35||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC31(YDL195W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.2||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN1(YLR430W)|FD-Score:5.92|P-value:1.63E-9|Clearance:2.35||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SFH1(YLR321C)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.55||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:RPP1(YHR062C)|FD-Score:6.15|P-value:3.93E-10|Clearance:2.35||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC31(YDL195W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.2||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN1(YLR430W)|FD-Score:5.92|P-value:1.63E-9|Clearance:2.35||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SFH1(YLR321C)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.55||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 714516
Download HOP data (tab-delimited text)  (excel)
Gene:ANP1(YEL036C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATG31(YDR022C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:EFM1(YHL039W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERF2(YLR246W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FMP52(YER004W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GEA2(YEL022W)|FD-Score:7.06|P-value:8.18E-13||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HOP1(YIL072W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:KRE1(YNL322C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:MDM31(YHR194W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MRPL38(YKL170W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MST1(YKL194C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NCA2(YPR155C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:PTR2(YKR093W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUF2(YPR042C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RIF2(YLR453C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPN4(YDL020C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SHE9(YDR393W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SMY1(YKL079W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SPE4(YLR146C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SPO12(YHR152W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SRO9(YCL037C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TFS1(YLR178C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress Gene:VPS65(YLR322W_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAR1(YPL239W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YCL046W(YCL046W_d)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR043C(YCR043C_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YGR273C(YGR273C_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL015W-A(YHL015W-A_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative protein of unknown function Gene:YHR022C(YHR022C_p)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YKR051W(YKR051W_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function Gene:YMR166C(YMR166C_p)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YNL058C(YNL058C_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YNR066C(YNR066C_p)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YTP1(YNL237W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ANP1(YEL036C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATG31(YDR022C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:EFM1(YHL039W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERF2(YLR246W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FMP52(YER004W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GEA2(YEL022W)|FD-Score:7.06|P-value:8.18E-13||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HOP1(YIL072W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:KRE1(YNL322C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:MDM31(YHR194W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MRPL38(YKL170W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MST1(YKL194C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NCA2(YPR155C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:PTR2(YKR093W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUF2(YPR042C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RIF2(YLR453C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPN4(YDL020C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SHE9(YDR393W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SMY1(YKL079W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SPE4(YLR146C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SPO12(YHR152W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SRO9(YCL037C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TFS1(YLR178C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress Gene:VPS65(YLR322W_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAR1(YPL239W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YCL046W(YCL046W_d)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR043C(YCR043C_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YGR273C(YGR273C_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL015W-A(YHL015W-A_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative protein of unknown function Gene:YHR022C(YHR022C_p)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YKR051W(YKR051W_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function Gene:YMR166C(YMR166C_p)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YNL058C(YNL058C_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YNR066C(YNR066C_p)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YTP1(YNL237W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C6.153.93E-102.35RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR430W5.921.63E-92.35SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YDL195W3.571.80E-40.20SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YLR321C3.373.82E-40.55SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YBL041W2.820.002430.15PRE7Beta 6 subunit of the 20S proteasome
YFL045C2.670.003832.34E-4SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YLR274W2.670.003830.03MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YGR030C2.630.004230.02POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR082C2.610.004510.09DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YDR081C2.520.005790.17PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YFL034C-B2.360.009190.04MOB2Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
YGR073C_d2.320.010200.01YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YJR046W2.310.010400.00TAH11DNA replication licensing factor, required for pre-replication complex assembly
YHR170W2.310.010501.38E-4NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W2.310.010500.01LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL022W7.068.18E-13GEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
YIL072W4.532.93E-6HOP1Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments
YPR155C4.211.25E-5NCA2Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
YHR152W4.181.43E-5SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
YKR093W4.131.85E-5PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YDR393W4.013.07E-5SHE9Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex
YNL058C_p3.963.68E-5YNL058C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene
YMR166C_p3.934.23E-5YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YHR022C_p3.865.77E-5YHR022C_pPutative protein of unknown function; YHR022C is not an essential gene
YKL170W3.826.66E-5MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YPL274W3.797.53E-5SAM3High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
YLR453C3.711.02E-4RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YCL001W-B_p3.681.15E-4YCL001W-B_pPutative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication
YKR051W_p3.651.29E-4YKR051W_pPutative protein of unknown function
YCR043C_p3.621.45E-4YCR043C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene

GO enrichment analysis for SGTC_1485
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1533.22E-32SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0759494RPP1 & pyrimidine depletion
0.1338.85E-25SGTC_14873970-0795 2.8 μMChemDiv (Drug-like library)37651030.0434783DNA intercalators
0.1339.54E-25SGTC_1468k284-1086 134.0 μMChemDiv (Drug-like library)159964570.0632911DNA intercalators
0.1226.30E-21SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0714286
0.1133.54E-18SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.0595238
0.1133.20E-18SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.232877
0.1102.77E-17SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.113924RPP1 & pyrimidine depletion
0.1071.32E-16SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.113924RNA pol III & RNase P/MRP
0.1071.68E-16SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.0724638
0.1001.57E-14SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.111111RPP1 & pyrimidine depletion
0.0992.05E-14SGTC_264promethazine 12.5 mMMiscellaneous60140.078125DNA intercalators
0.0993.18E-14SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.119403RPP1 & pyrimidine depletion
0.0983.81E-14SGTC_20245144227 134.0 μMChembridge (Fragment library)34752110.0555556
0.0979.36E-14SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.111111
0.0961.35E-13SGTC_1115galangin 695.7 nMTimTec (Natural product library)52816160.0428571RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_530973-002339.98 μM0.310345674768ChemDiv (Drug-like library)261.33943.3714RNA processing & uracil transport
SGTC_2113455-0679143.5 μM0.288136748649ChemDiv (Drug-like library)220.221282.87314
SGTC_2406ethylparaben405.7 μM0.288434Miscellaneous166.17391.79213
SGTC_2626ethyl orsellinate100 μM0.27777875653Microsource (Natural product library)196.199882.03624
SGTC_20965468907200 μM0.271186759785Chembridge (Fragment library)236.293480.44925
SGTC_9483448-029575.3 μM0.263158678569ChemDiv (Drug-like library)223.311343.70112
SGTC_23017769476200 μM0.258065977820Chembridge (Fragment library)253.343841.85915
SGTC_2506ethyl hematommate54.16 μM0.2542373940691Microsource (Natural product library)224.209981.79525RPP1 & pyrimidine depletion
SGTC_1746st03811742 μM0.2533332898218TimTec (Natural product derivative library)476.542623.20818
SGTC_12220366-00741.18 μM0.25286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4