3970-0793

2-(4-bromophenyl)-3a,4,7,7a-tetrahydro-octahydro-1H-4,7-epoxyisoindole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1486
Screen concentration 4.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 2844330
SMILES C1=CC2C3C(C1O2)C(=O)N(C3=O)C4=CC=C(C=C4)Br
Standardized SMILES Brc1ccc(cc1)N2C(=O)C3C4OC(C=C4)C3C2=O
Molecular weight 320.1381
ALogP 1.59
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.06
% growth inhibition (Hom. pool) 2.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2844330
Download HIP data (tab-delimited text)  (excel)
Gene:ERV1(YGR029W)|FD-Score:3.86|P-value:5.72E-5|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:MAS1(YLR163C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.17||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NSE4(YDL105W)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.17||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:POP3(YNL282W)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP45(YAL032C)|FD-Score:3.13|P-value:8.73E-4|Clearance:0.03||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:SAD1(YFR005C)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.22||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SCM3(YDL139C)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.17||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC23(YPR181C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.01||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SMD1(YGR074W)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SNU23(YDL098C)|FD-Score:4.29|P-value:8.89E-6|Clearance:0.43||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:ERV1(YGR029W)|FD-Score:3.86|P-value:5.72E-5|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:MAS1(YLR163C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.17||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NSE4(YDL105W)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.17||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:POP3(YNL282W)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP45(YAL032C)|FD-Score:3.13|P-value:8.73E-4|Clearance:0.03||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:SAD1(YFR005C)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.22||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SCM3(YDL139C)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.17||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC23(YPR181C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.01||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SMD1(YGR074W)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SNU23(YDL098C)|FD-Score:4.29|P-value:8.89E-6|Clearance:0.43||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2844330
Download HOP data (tab-delimited text)  (excel)
Gene:AQY2(YLL052C)|FD-Score:5.52|P-value:1.73E-8||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:BRE1(YDL074C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:COS111(YBR203W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CUS2(YNL286W)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:ECM12(YHR021W-A_p)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity Gene:FUI1(YBL042C)|FD-Score:7.66|P-value:9.32E-15||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GET1(YGL020C)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GLK1(YCL040W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:IMP2'(YIL154C)|FD-Score:4.8|P-value:8.09E-7||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISU1(YPL135W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:MDM36(YPR083W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MIC23(YBL107C_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MSG5(YNL053W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:PHO91(YNR013C)|FD-Score:5.72|P-value:5.33E-9||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PTH2(YBL057C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RPL21A(YBR191W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SAP30(YMR263W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAT4(YCR008W)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SDL1(YIL167W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SRL4(YPL033C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:STE50(YCL032W)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SYS1(YJL004C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TSR3(YOR006C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:TUB3(YML124C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:YBL029C-A(YBL029C-A_p)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YFL032W(YFL032W_d)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YJL070C(YJL070C_p)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKL069W(YKL069W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLR271W(YLR271W_p)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMR265C(YMR265C_p)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Putative protein of unknown function Gene:YPK3(YBR028C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:AQY2(YLL052C)|FD-Score:5.52|P-value:1.73E-8||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:BRE1(YDL074C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:COS111(YBR203W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CUS2(YNL286W)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:ECM12(YHR021W-A_p)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity Gene:FUI1(YBL042C)|FD-Score:7.66|P-value:9.32E-15||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GET1(YGL020C)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GLK1(YCL040W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:IMP2'(YIL154C)|FD-Score:4.8|P-value:8.09E-7||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISU1(YPL135W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:MDM36(YPR083W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MIC23(YBL107C_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MSG5(YNL053W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:PHO91(YNR013C)|FD-Score:5.72|P-value:5.33E-9||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PTH2(YBL057C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RPL21A(YBR191W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SAP30(YMR263W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAT4(YCR008W)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SDL1(YIL167W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SRL4(YPL033C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:STE50(YCL032W)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SYS1(YJL004C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TSR3(YOR006C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:TUB3(YML124C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:YBL029C-A(YBL029C-A_p)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YFL032W(YFL032W_d)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YJL070C(YJL070C_p)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKL069W(YKL069W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLR271W(YLR271W_p)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMR265C(YMR265C_p)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Putative protein of unknown function Gene:YPK3(YBR028C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL098C4.298.89E-60.43SNU23Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
YGR029W3.865.72E-50.00ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YDL105W3.865.77E-50.17NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YLR163C3.691.12E-40.17MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YFR005C3.522.15E-40.22SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YDL139C3.304.90E-40.17SCM3Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP
YAL032C3.138.73E-40.03PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YPR181C3.109.72E-40.01SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YDR052C3.090.001010.01DBF4Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress
YPR082C3.080.001030.00DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YLR229C3.080.001040.04CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YPR108W3.040.001170.00RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YLR230W_d3.040.001190.02YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YFL034C-B3.020.001260.02MOB2Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
YGL065C3.000.001340.02ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL042C7.669.32E-15FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YNR013C5.725.33E-9PHO91Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
YLL052C5.521.73E-8AQY2Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
YIL154C4.808.09E-7IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YMR263W4.532.99E-6SAP30Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
YNL053W4.221.21E-5MSG5Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p
YBL057C4.042.62E-5PTH2One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
YMR265C_p3.963.67E-5YMR265C_pPutative protein of unknown function
YPL135W3.934.32E-5ISU1Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
YGL020C3.885.29E-5GET1Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
YCR008W3.768.56E-5SAT4Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
YJL070C_p3.758.84E-5YJL070C_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
YFL032W_d3.739.56E-5YFL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
YPL001W3.433.06E-4HAT1Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
YBR028C3.423.13E-4YPK3An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner

GO enrichment analysis for SGTC_1486
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1506.94E-31SGTC_2234tolnaftate 200.0 μMMiscellaneous55100.0483871
0.1202.40E-20SGTC_3803970-0968 6.0 μMChemDiv (Drug-like library)28916010.235294
0.1103.23E-17SGTC_400oxethazaine 37.5 μMMiscellaneous46210.0666667calcium & mitochondrial duress
0.0984.82E-14SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0526316calcium & mitochondrial duress
0.0971.01E-13SGTC_2523spermine 47.7 μMMicrosource (Natural product library)1103NAcalcium & mitochondrial duress
0.0961.54E-13SGTC_960929-0093 37.3 μMChemDiv (Drug-like library)69915790.078125calcium & mitochondrial duress
0.0943.84E-13SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0422535calcium & mitochondrial duress
0.0921.11E-12SGTC_3883',4'-dichloroisocoumarin 16.7 μMMiscellaneous16090.136364
0.0871.88E-11SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.0843373calcium & mitochondrial duress
0.0872.39E-11SGTC_330986-0033 35.8 μMChemDiv (Drug-like library)28480820.0615385calcium & mitochondrial duress
0.0822.99E-10SGTC_12600700-1617 7.8 μMChemDiv (Drug-like library)28530780.0740741calcium & mitochondrial duress
0.0808.22E-10SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.0684932calcium & mitochondrial duress
0.0809.45E-10SGTC_1690st023502 41.2 μMTimTec (Natural product derivative library)242074820.111111calcium & mitochondrial duress
0.0765.39E-9SGTC_6090986-0026 86.3 μMChemDiv (Drug-like library)28486100.0677966calcium & mitochondrial duress
0.0759.30E-9SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.0555556

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2103537813810 μM0.6875244142Chembridge (Fragment library)241.242040.84403
SGTC_14873970-07952.82 μM0.6666673765103ChemDiv (Drug-like library)320.13811.59203DNA intercalators
SGTC_1903970-07903.72 μM0.6216222832352ChemDiv (Drug-like library)286.23960.73805
SGTC_215856571233.62 μM0.5945952858827Chembridge (Fragment library)269.29521.81603
SGTC_9963970-07991.8 μM0.5609766615648ChemDiv (Drug-like library)299.321181.704
SGTC_12790874-059065.2 μM0.5121953523074ChemDiv (Drug-like library)410.260643.99503
SGTC_2200660060421.91 μM0.52904777Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_216156625048.51 μM0.4871792859334Chembridge (Fragment library)275.68711.50803tubulin folding & SWR complex
SGTC_21735763948167 μM0.4634152867177Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_20985376439882.5 nM0.3617022844116Chembridge (Fragment library)255.268621.04903