4089-0339

5-chloro-2-(4-chloro-3-methylphenyl)-4-methyl-1,2-thiazol-3-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1490
Screen concentration 670.0 nM
Source ChemDiv (Drug-like library)
PubChem CID 742542
SMILES CC1=C(C=CC(=C1)N2C(=O)C(=C(S2)Cl)C)Cl
Standardized SMILES CC1=C(Cl)SN(C1=O)c2ccc(Cl)c(C)c2
Molecular weight 274.1663
ALogP 4.19
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.8
% growth inhibition (Hom. pool) 4.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 742542
Download HIP data (tab-delimited text)  (excel)
Gene:FAS2(YPL231W)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GPI15(YNL038W)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:PRE7(YBL041W)|FD-Score:3.31|P-value:4.66E-4|Clearance:0.16||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RIA1(YNL163C)|FD-Score:-3.87|P-value:5.41E-5|Clearance:0||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RPA43(YOR340C)|FD-Score:5.1|P-value:1.68E-7|Clearance:0.87||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPT3(YDR394W)|FD-Score:4.96|P-value:3.55E-7|Clearance:0.87||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT6(YGL048C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.12||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRP4(YHR069C)|FD-Score:4.72|P-value:1.21E-6|Clearance:0.87||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SMD2(YLR275W)|FD-Score:3.15|P-value:8.08E-4|Clearance:0.05||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TFA2(YKR062W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TRM5(YHR070W)|FD-Score:-3.78|P-value:7.74E-5|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TUB1(YML085C)|FD-Score:3.85|P-value:5.99E-5|Clearance:0.29||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP15(YMR093W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR115C(YGR115C_d)|FD-Score:3.41|P-value:3.31E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:FAS2(YPL231W)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GPI15(YNL038W)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:PRE7(YBL041W)|FD-Score:3.31|P-value:4.66E-4|Clearance:0.16||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RIA1(YNL163C)|FD-Score:-3.87|P-value:5.41E-5|Clearance:0||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RPA43(YOR340C)|FD-Score:5.1|P-value:1.68E-7|Clearance:0.87||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPT3(YDR394W)|FD-Score:4.96|P-value:3.55E-7|Clearance:0.87||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT6(YGL048C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.12||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRP4(YHR069C)|FD-Score:4.72|P-value:1.21E-6|Clearance:0.87||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SMD2(YLR275W)|FD-Score:3.15|P-value:8.08E-4|Clearance:0.05||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TFA2(YKR062W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TRM5(YHR070W)|FD-Score:-3.78|P-value:7.74E-5|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TUB1(YML085C)|FD-Score:3.85|P-value:5.99E-5|Clearance:0.29||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP15(YMR093W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR115C(YGR115C_d)|FD-Score:3.41|P-value:3.31E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 742542
Download HOP data (tab-delimited text)  (excel)
Gene:AQY1(YPR192W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:CDC73(YLR418C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM25(YJL201W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ERI1(YPL096C-A)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:GID7(YCL039W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GUP1(YGL084C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:ICT1(YLR099C)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:MDR1(YGR100W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MET18(YIL128W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:PET112(YBL080C)|FD-Score:7.95|P-value:9.51E-16||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:RAD51(YER095W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPL34A(YER056C-A)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SCP160(YJL080C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SUR4(YLR372W)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC3(YAL011W)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TDP1(YBR223C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TEX1(YNL253W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TSC3(YBR058C-A)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VCX1(YDL128W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:YCR085W(YCR085W_d)|FD-Score:-4.53|P-value:2.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL017W(YHL017W_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIL163C(YIL163C_p)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKE4(YIL023C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLR050C(YLR050C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YPL068C(YPL068C_p)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YSW1(YBR148W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:AQY1(YPR192W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:CDC73(YLR418C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM25(YJL201W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ERI1(YPL096C-A)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:GID7(YCL039W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GUP1(YGL084C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:ICT1(YLR099C)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:MDR1(YGR100W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MET18(YIL128W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:PET112(YBL080C)|FD-Score:7.95|P-value:9.51E-16||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:RAD51(YER095W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPL34A(YER056C-A)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SCP160(YJL080C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SUR4(YLR372W)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC3(YAL011W)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TDP1(YBR223C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TEX1(YNL253W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TSC3(YBR058C-A)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VCX1(YDL128W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:YCR085W(YCR085W_d)|FD-Score:-4.53|P-value:2.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL017W(YHL017W_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIL163C(YIL163C_p)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKE4(YIL023C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLR050C(YLR050C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YPL068C(YPL068C_p)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YSW1(YBR148W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR340C5.101.68E-70.87RPA43RNA polymerase I subunit A43
YDR394W4.963.55E-70.87RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YHR069C4.721.21E-60.87RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YML085C3.855.99E-50.29TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YMR093W3.551.91E-48.48E-4UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YBR154C3.551.91E-40.15RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YGR115C_d3.413.31E-40.09YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YBL041W3.314.66E-40.16PRE7Beta 6 subunit of the 20S proteasome
YLR275W3.158.08E-40.05SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YGL048C3.109.72E-40.12RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YGR116W2.980.001430.06SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YBR202W2.930.001710.02MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YLR215C2.910.001830.08CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YCL052C2.820.002380.01PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YJL001W2.820.002420.04PRE3Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL080C7.959.51E-16PET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
YLR418C5.462.32E-8CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YAL011W4.721.20E-6SWC3Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
YIL163C_p4.356.69E-6YIL163C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YPL096C-A4.241.13E-5ERI1Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
YER056C-A4.161.61E-5RPL34ARibosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
YLR099C4.131.80E-5ICT1Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication
YBR148W3.904.79E-5YSW1Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane
YGR100W3.865.65E-5MDR1Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
YLR372W3.797.44E-5SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YJL080C3.768.54E-5SCP160Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
YLR050C_p3.651.32E-4YLR050C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene
YDL128W3.601.60E-4VCX1Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
YHL017W_p3.571.76E-4YHL017W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
YOR014W3.561.89E-4RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A

GO enrichment analysis for SGTC_1490
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1426.04E-28SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0714286
0.1343.37E-25SGTC_2740felodipine 17.1 μMMiscellaneous33330.138462
0.1292.17E-23SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0666667TSC3-RPN4
0.1249.74E-22SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.0684932
0.1241.10E-21SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.179104TSC3-RPN4
0.1211.10E-20SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0877193TSC3-RPN4
0.1202.65E-20SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.115942
0.1193.43E-20SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.114286
0.1164.34E-19SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0545455
0.1164.46E-19SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0819672
0.1134.18E-18SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0714286
0.1071.24E-16SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.142857TSC3-RPN4
0.1062.64E-16SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0508475TSC3-RPN4
0.1048.39E-16SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.2TSC3-RPN4
0.1032.24E-15SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0555556TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21405586273200 μM0.348837854421Chembridge (Fragment library)223.65562.1402RSC complex & mRNA processing
SGTC_21075363541170.16 μM0.297872244052Chembridge (Fragment library)199.63421.81812
SGTC_20685226020147 μM0.2666671810509Chembridge (Fragment library)195.602441.82112
SGTC_215856571233.62 μM0.2653062858827Chembridge (Fragment library)269.29521.81603
SGTC_2246729350791.56 μM0.26887656Chembridge (Fragment library)244.245980.99413
SGTC_2001402350257.66 μM0.23913108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_1898st06019918.6 μM0.230769688866TimTec (Natural product derivative library)219.623842.37703
SGTC_3028908777849.47 μM0.23076917217395Chembridge (Drug-like library)373.876464.04304
SGTC_3050909351349.47 μM0.23076925236664Chembridge (Drug-like library)352.898763.86913
SGTC_1882561564320 μM0.2272732253075Miscellaneous392.8979635.84215TRP & mitochondrial translation