4138-0063

8-fluoro-4-piperidin-1-yl-5H-pyrimido[5,4-b]indole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1491
Screen concentration 26.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 23883227
SMILES C1CCN(CC1)C2=NC=NC3=C2NC4=C3C=C(C=C4)F
Standardized SMILES Fc1ccc2[nH]c3c(ncnc3c2c1)N4CCCCC4
Molecular weight 270.3048
ALogP 3.8
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.8
% growth inhibition (Hom. pool) 4.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 23883227
Download HIP data (tab-delimited text)  (excel)
Gene:BCD1(YHR040W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.35||SGD DESC:Essential protein required for the accumulation of box C/D snoRNA Gene:BIG1(YHR101C)|FD-Score:3.18|P-value:7.29E-4|Clearance:0.1||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:MED4(YOR174W)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET4(YNL103W)|FD-Score:9.48|P-value:1.27E-21|Clearance:4.39||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MVD1(YNR043W)|FD-Score:5.09|P-value:1.79E-7|Clearance:1.03||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:RNR2(YJL026W)|FD-Score:4.06|P-value:2.42E-5|Clearance:0.44||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:SFH1(YLR321C)|FD-Score:-3.14|P-value:8.39E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SRM1(YGL097W)|FD-Score:-3.18|P-value:7.27E-4|Clearance:0||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TRR1(YDR353W)|FD-Score:-3.47|P-value:2.65E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:TRS31(YDR472W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0.09||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:BCD1(YHR040W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.35||SGD DESC:Essential protein required for the accumulation of box C/D snoRNA Gene:BIG1(YHR101C)|FD-Score:3.18|P-value:7.29E-4|Clearance:0.1||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:MED4(YOR174W)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET4(YNL103W)|FD-Score:9.48|P-value:1.27E-21|Clearance:4.39||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MVD1(YNR043W)|FD-Score:5.09|P-value:1.79E-7|Clearance:1.03||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:RNR2(YJL026W)|FD-Score:4.06|P-value:2.42E-5|Clearance:0.44||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:SFH1(YLR321C)|FD-Score:-3.14|P-value:8.39E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SRM1(YGL097W)|FD-Score:-3.18|P-value:7.27E-4|Clearance:0||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TRR1(YDR353W)|FD-Score:-3.47|P-value:2.65E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:TRS31(YDR472W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0.09||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 23883227
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AQY1(YPR192W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:BIM1(YER016W)|FD-Score:4|P-value:3.14E-5||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTS1(YPL069C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:ECM18(YDR125C)|FD-Score:-3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GUP1(YGL084C)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HUB1(YNR032C-A)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:MAL11(YGR289C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MRP49(YKL167C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL4(YLR439W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:NUP84(YDL116W)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:RNQ1(YCL028W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RRI1(YDL216C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SLY41(YOR307C)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SMM1(YNR015W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SNA2(YDR525W-A)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SRB2(YHR041C)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SWI5(YDR146C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRK1(YJL129C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:7.28|P-value:1.67E-13||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VPS45(YGL095C)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:WHI2(YOR043W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:WSS1(YHR134W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAP1801(YHR161C)|FD-Score:4.66|P-value:1.62E-6||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YGL007C-A(YGL007C-A_p)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YNL155W(YNL155W_p)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:AAT2(YLR027C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AQY1(YPR192W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:BIM1(YER016W)|FD-Score:4|P-value:3.14E-5||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTS1(YPL069C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:ECM18(YDR125C)|FD-Score:-3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GUP1(YGL084C)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HUB1(YNR032C-A)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:MAL11(YGR289C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MRP49(YKL167C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL4(YLR439W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:NUP84(YDL116W)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:RNQ1(YCL028W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RRI1(YDL216C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SLY41(YOR307C)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SMM1(YNR015W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SNA2(YDR525W-A)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SRB2(YHR041C)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SWI5(YDR146C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRK1(YJL129C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:7.28|P-value:1.67E-13||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VPS45(YGL095C)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:WHI2(YOR043W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:WSS1(YHR134W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAP1801(YHR161C)|FD-Score:4.66|P-value:1.62E-6||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YGL007C-A(YGL007C-A_p)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YNL155W(YNL155W_p)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL103W9.481.27E-214.39MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YNR043W5.091.79E-71.03MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YJL026W4.062.42E-50.43RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YDR472W3.631.43E-40.09TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YHR040W3.532.04E-40.35BCD1Essential protein required for the accumulation of box C/D snoRNA
YHR101C3.187.29E-40.10BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YIR011C3.080.001040.07STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YDL150W3.010.001320.13RPC53RNA polymerase III subunit C53
YPL131W2.870.002030.07RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YHR196W2.810.002490.07UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YNL002C2.740.003080.03RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YBL004W2.710.003360.02UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YML092C2.690.003540.01PRE8Alpha 2 subunit of the 20S proteasome
YGL225W2.690.003610.01VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YJL087C2.680.003720.16TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML013W7.281.67E-13UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YHR161C4.661.62E-6YAP1801Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
YDL116W4.641.71E-6NUP84Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
YDR146C4.582.32E-6SWI5Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
YLR027C4.503.37E-6AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
YBR069C4.356.85E-6TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YGL095C4.327.96E-6VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YPL069C4.161.57E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YHR134W4.072.37E-5WSS1Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
YGL007C-A_p4.032.79E-5YGL007C-A_pPutative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding
YER016W4.003.14E-5BIM1Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
YGR289C3.875.42E-5MAL11Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
YCL028W3.855.89E-5RNQ1[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate
YOR307C3.758.84E-5SLY41Protein involved in ER-to-Golgi transport
YGL084C3.749.22E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport

GO enrichment analysis for SGTC_1491
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1202.64E-20SGTC_33299141233 27.6 μMChembridge (Drug-like library)49091010.123457
0.1093.94E-17SGTC_33239138564 53.9 μMChembridge (Drug-like library)439123130.0555556
0.1015.59E-15SGTC_33289140624 29.0 μMChembridge (Drug-like library)49043920.126761
0.0993.10E-14SGTC_24315490079 200.0 μMMiscellaneous57223450.056338
0.0895.89E-12SGTC_21425622915 118.5 μMChembridge (Fragment library)53221960.0952381
0.0841.05E-10SGTC_155k072-0202 43.6 μMChemDiv (Drug-like library)58577370.0526316
0.0831.64E-10SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0571429
0.0805.90E-10SGTC_1593970-0959 50.0 μMChemDiv (Drug-like library)66156530.0465116
0.0791.21E-9SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.0806452heat shock/prefoldin
0.0791.42E-9SGTC_30049076122 71.4 μMChembridge (Drug-like library)447825510.0657895
0.0782.30E-9SGTC_20315180455 200.0 μMChembridge (Fragment library)299490.0625
0.0741.35E-8SGTC_2395hr heat shock (37°C) + mitomycin C 819.4 μMMiscellaneous57460.0344828heat shock/prefoldin
0.0723.12E-8SGTC_2605digitonin 300.0 nMTimTec (Pure natural product library)27350100.00806452
0.0714.67E-8SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.0266667heat shock/prefoldin
0.0714.95E-8SGTC_15956-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 67.5 μMTimTec (Pure natural product library)6887880.0526316

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_5953909-800373.7 μM0.285714699517ChemDiv (Drug-like library)259.369883.65704
SGTC_11961588-0294184 μM0.2539686144269ChemDiv (Drug-like library)355.4125834.57918
SGTC_22587960471200 μM0.252974708Chembridge (Fragment library)298.834762.64715
SGTC_11483909-766327.7 μM0.2459024084526ChemDiv (Drug-like library)320.38833.70205
SGTC_2049526283167.19 μM0.233333763417Chembridge (Fragment library)272.3206832.4805
SGTC_3219913111249.47 μM0.22950817612988Chembridge (Drug-like library)298.3546633.4513Golgi
SGTC_3259913848549.47 μM0.22950817646153Chembridge (Drug-like library)334.4083633.15315
SGTC_1516st013856110 μM0.2222225281404TimTec (Pure natural product library)182.22122.45511
SGTC_2696st07769174.32 μM0.220339757133TimTec (Natural product derivative library)200.279542.83611
SGTC_3010908008971.43 μM0.21739117027233Chembridge (Drug-like library)378.2387833.49714