4158-0558

1-(2-hydroxy-4-methylphenyl)-2-(7-phenyl-1,4-diazepan-5-ylidene)ethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1492
Screen concentration 28.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 3967268
SMILES CC1=CC(=C(C=C1)C(=O)C=C2CC(NCCN2)C3=CC=CC=C3)O
Standardized SMILES Cc1ccc(C(=CC2=NCCNC(C2)c3ccccc3)O)c(O)c1
Molecular weight 322.4009
ALogP 2.69
H-bond donor count 3
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.38
% growth inhibition (Hom. pool) 6.57


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3967268
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.07||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:ESF2(YNR054C)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.39||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:LSG1(YGL099W)|FD-Score:6.95|P-value:1.87E-12|Clearance:3.12||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.49||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPF2(YKR081C)|FD-Score:3.83|P-value:6.39E-5|Clearance:0.13||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL25(YOL127W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPS2(YGL123W)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RRP9(YPR137W)|FD-Score:-4.57|P-value:2.47E-6|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SLD3(YGL113W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.02||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:-3.18|P-value:7.42E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:CCT3(YJL014W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.07||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:ESF2(YNR054C)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.39||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:LSG1(YGL099W)|FD-Score:6.95|P-value:1.87E-12|Clearance:3.12||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.49||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPF2(YKR081C)|FD-Score:3.83|P-value:6.39E-5|Clearance:0.13||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL25(YOL127W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPS2(YGL123W)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RRP9(YPR137W)|FD-Score:-4.57|P-value:2.47E-6|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SLD3(YGL113W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.02||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:-3.18|P-value:7.42E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3967268
Download HOP data (tab-delimited text)  (excel)
Gene:ANS1(YHR126C_p)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARO9(YHR137W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:CHA1(YCL064C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CSH1(YBR161W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CSM2(YIL132C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:FAR11(YNL127W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FMP33(YJL161W_p)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRD1(YEL047C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GTR1(YML121W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HST1(YOL068C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HXT3(YDR345C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:ICS2(YBR157C)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IRC14(YOR135C_d)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC21(YMR073C)|FD-Score:5.25|P-value:7.50E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:MDM20(YOL076W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:NPR2(YEL062W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NUP84(YDL116W)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PAH1(YMR165C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PBS2(YJL128C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDR18(YNR070W_p)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PHO89(YBR296C)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIG2(YIL045W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PUT4(YOR348C)|FD-Score:7.04|P-value:9.28E-13||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RBK1(YCR036W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Putative ribokinase Gene:RCY1(YJL204C)|FD-Score:7.34|P-value:1.10E-13||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REI1(YBR267W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RPN4(YDL020C)|FD-Score:5.81|P-value:3.22E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT13(YER066W_p)|FD-Score:-3.36|P-value:3.97E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:SGS1(YMR190C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SLH1(YGR271W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SUL2(YLR092W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWR1(YDR334W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA20(YER007C-A)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UGA2(YBR006W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VHC1(YBR235W_p)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VID22(YLR373C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS27(YNR006W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:XBP1(YIL101C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR455C(YDR455C_d)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR541C(YDR541C_p)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YLR224W(YLR224W_p)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YLR312C(YLR312C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function Gene:YNL228W(YNL228W_d)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNR065C(YNR065C_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR325W(YOR325W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:ANS1(YHR126C_p)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARO9(YHR137W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:CHA1(YCL064C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CSH1(YBR161W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CSM2(YIL132C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:FAR11(YNL127W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FMP33(YJL161W_p)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRD1(YEL047C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GTR1(YML121W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HST1(YOL068C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HXT3(YDR345C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:ICS2(YBR157C)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IRC14(YOR135C_d)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC21(YMR073C)|FD-Score:5.25|P-value:7.50E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:MDM20(YOL076W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:NPR2(YEL062W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NUP84(YDL116W)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PAH1(YMR165C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PBS2(YJL128C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDR18(YNR070W_p)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PHO89(YBR296C)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIG2(YIL045W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PUT4(YOR348C)|FD-Score:7.04|P-value:9.28E-13||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RBK1(YCR036W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Putative ribokinase Gene:RCY1(YJL204C)|FD-Score:7.34|P-value:1.10E-13||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REI1(YBR267W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RPN4(YDL020C)|FD-Score:5.81|P-value:3.22E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT13(YER066W_p)|FD-Score:-3.36|P-value:3.97E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:SGS1(YMR190C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SLH1(YGR271W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SUL2(YLR092W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWR1(YDR334W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA20(YER007C-A)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UGA2(YBR006W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VHC1(YBR235W_p)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VID22(YLR373C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS27(YNR006W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:XBP1(YIL101C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR455C(YDR455C_d)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR541C(YDR541C_p)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YLR224W(YLR224W_p)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YLR312C(YLR312C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function Gene:YNL228W(YNL228W_d)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNR065C(YNR065C_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR325W(YOR325W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W6.951.87E-123.12LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YKR081C3.836.39E-50.13RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YGL116W3.701.07E-40.07CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YGL113W3.641.39E-40.02SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
YHR170W3.621.49E-40.49NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YNR054C3.138.72E-40.40ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YER112W2.740.003110.19LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YBR252W2.540.005480.06DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YOR046C2.480.006530.01DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p
YLR317W_d2.480.006660.02YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
YNL188W2.450.007120.03KAR1Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
YHR164C2.420.007660.02DNA2Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
YLR347C2.410.008020.02KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YDR331W2.390.008470.07GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YOR181W2.320.010300.02LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C7.341.10E-13RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YOR348C7.049.28E-13PUT4Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
YDL020C5.813.22E-9RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YMR073C5.257.50E-8IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YBR235W_p4.983.11E-7VHC1_pVacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
YBR267W4.671.49E-6REI1Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
YDL116W4.592.23E-6NUP84Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
YLR092W4.454.31E-6SUL2High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
YIL132C4.241.10E-5CSM2Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
YOR325W_d4.231.17E-5YOR325W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1
YDL010W4.231.17E-5GRX6Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
YCR036W4.062.46E-5RBK1Putative ribokinase
YJL161W_p3.993.27E-5FMP33_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBR006W3.953.95E-5UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YHR137W3.934.23E-5ARO9Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism

GO enrichment analysis for SGTC_1492
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1925.45E-50SGTC_1081sertraline 6.0 μMNIH Clinical Collection630090.13888960S ribosome export
0.1782.82E-43SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0606061
0.1742.21E-41SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.11111160S ribosome export
0.1697.18E-39SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.14634160S ribosome export
0.1697.78E-39SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.10256460S ribosome export
0.1604.33E-35SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.088888960S ribosome export
0.1561.83E-33SGTC_32499135183 49.5 μMChembridge (Drug-like library)49027790.12222260S ribosome export
0.1503.98E-31SGTC_13943474-0001 65.7 μMChemDiv (Drug-like library)50176660.144737endomembrane recycling
0.1506.12E-31SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.081632760S ribosome export
0.1491.28E-30SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.14084560S ribosome export
0.1452.56E-29SGTC_2699st077704 54.6 μMTimTec (Natural product derivative library)6458520.133333endomembrane recycling
0.1432.95E-28SGTC_1733st037357 54.9 μMTimTec (Natural product derivative library)12723390.09195460S ribosome export
0.1404.02E-27SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.063291160S ribosome export
0.1399.85E-27SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.068181860S ribosome export
0.1344.41E-25SGTC_2694st077685 81.9 μMTimTec (Natural product derivative library)28380960.153846endomembrane recycling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_24825981709142.65 μM0.261538877755Miscellaneous333.176624.14713PDR1
SGTC_13051181-0477144 μM0.2463776252086ChemDiv (Drug-like library)282.337023.39423
SGTC_7560508-199928.3 μM0.246753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_918glabranin770.73 nM0.236842124049TimTec (Natural product library)324.37044.47224
SGTC_2484580981812.04 μM0.2337662870548Miscellaneous355.407583.66915
SGTC_20214019055173 μM0.22580644827708Chembridge (Fragment library)163.216321.26112endomembrane recycling
SGTC_18233-hydroxy-2-naphthoic acid106 μM0.2241387104TimTec (Natural product derivative library)188.179422.12623
SGTC_23217390090138.78 μM0.223881649046Chembridge (Fragment library)214.263062.85423
SGTC_1691st01993384.6 μM0.2153856176082TimTec (Natural product derivative library)236.308344.67401
SGTC_2475581272815.03 μM0.214286776160Miscellaneous272.2710233.60414mitochondrial processes