4480-0877

N-(8-methyl-8-azabicyclo[3.2.1]oct-3-en-3-yl)-4-phenyl-N-(2-phenylethyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1499
Screen concentration 67.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 2861909
SMILES CN1C2CCC1C=C(C2)N(CCC3=CC=CC=C3)C(=O)C4=CC=C(C=C4)C5=CC=CC=C5
Standardized SMILES CN1C2CCC1C=C(C2)N(CCc3ccccc3)C(=O)c4ccc(cc4)c5ccccc5
Molecular weight 422.5613
ALogP 5.31
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.89
% growth inhibition (Hom. pool) 2.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2861909
Download HIP data (tab-delimited text)  (excel)
Gene:DIB1(YPR082C)|FD-Score:-3.19|P-value:7.04E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:GPI11(YDR302W)|FD-Score:-3.2|P-value:6.76E-4|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:NSE1(YLR007W)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.04||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PRE3(YJL001W)|FD-Score:-3.1|P-value:9.69E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPB2(YOR151C)|FD-Score:-3.85|P-value:5.83E-5|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPN7(YPR108W)|FD-Score:-3.72|P-value:9.81E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RTS2(YOR077W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.8||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SEC4(YFL005W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.1||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SFH1(YLR321C)|FD-Score:-3.53|P-value:2.12E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:YPL142C(YPL142C_d)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:DIB1(YPR082C)|FD-Score:-3.19|P-value:7.04E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:GPI11(YDR302W)|FD-Score:-3.2|P-value:6.76E-4|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:NSE1(YLR007W)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.04||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PRE3(YJL001W)|FD-Score:-3.1|P-value:9.69E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPB2(YOR151C)|FD-Score:-3.85|P-value:5.83E-5|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPN7(YPR108W)|FD-Score:-3.72|P-value:9.81E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RTS2(YOR077W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.8||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SEC4(YFL005W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.1||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SFH1(YLR321C)|FD-Score:-3.53|P-value:2.12E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:YPL142C(YPL142C_d)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2861909
Download HOP data (tab-delimited text)  (excel)
Gene:AIR2(YDL175C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALK1(YGL021W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:CAN1(YEL063C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CLN1(YMR199W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COX5A(YNL052W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:GIR2(YDR152W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:HOP1(YIL072W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:NBP2(YDR162C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PRS1(YKL181W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RCK2(YLR248W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RKM5(YLR137W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RMT2(YDR465C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL14A(YKL006W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL14B(YHL001W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL2B(YIL018W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RTA1(YGR213C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SNA4(YDL123W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:TMA108(YIL137C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TMN2(YDR107C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TRX2(YGR209C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBA4(YHR111W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UME6(YDR207C)|FD-Score:5.98|P-value:1.13E-9||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VPS20(YMR077C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS63(YLR261C_d)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VTC1(YER072W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YBR197C(YBR197C_p)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCL001W-B(YCL001W-B_p)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDR109C(YDR109C_p)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Putative kinase Gene:YIL024C(YIL024C_p)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIR014W(YIR014W_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YJR015W(YJR015W_p)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YOR292C(YOR292C_p)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPS7(YDR349C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT6(YLR262C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AIR2(YDL175C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALK1(YGL021W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:CAN1(YEL063C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CLN1(YMR199W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COX5A(YNL052W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:GIR2(YDR152W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:HOP1(YIL072W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:NBP2(YDR162C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PRS1(YKL181W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RCK2(YLR248W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RKM5(YLR137W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RMT2(YDR465C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL14A(YKL006W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL14B(YHL001W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL2B(YIL018W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RTA1(YGR213C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SNA4(YDL123W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:TMA108(YIL137C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TMN2(YDR107C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TRX2(YGR209C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBA4(YHR111W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UME6(YDR207C)|FD-Score:5.98|P-value:1.13E-9||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VPS20(YMR077C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS63(YLR261C_d)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VTC1(YER072W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YBR197C(YBR197C_p)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCL001W-B(YCL001W-B_p)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDR109C(YDR109C_p)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Putative kinase Gene:YIL024C(YIL024C_p)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIR014W(YIR014W_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YJR015W(YJR015W_p)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YOR292C(YOR292C_p)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPS7(YDR349C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT6(YLR262C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR077W4.181.47E-50.80RTS2Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
YFL005W3.373.71E-40.10SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YLR007W3.275.39E-40.04NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YPL142C_d3.236.19E-40.22YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YJR141W_p3.010.001320.16YJR141W_pEssential protein of unknown function
YMR281W2.840.002230.03GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YOR103C2.810.002450.05OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YLR198C_d2.770.002840.09YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YIR006C2.670.003770.01PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YOL134C_d2.660.003930.01YOL134C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
YMR112C2.640.004100.03MED11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
YMR197C2.610.004480.04VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YGL225W2.570.005060.03VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YER008C2.540.005570.05SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YNL038W2.490.006370.08GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR207C5.981.13E-9UME6Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p
YMR199W4.731.10E-6CLN1G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
YIR014W_p4.611.98E-6YIR014W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene
YIL024C_p4.102.11E-5YIL024C_pPutative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p
YGR209C4.072.40E-5TRX2Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
YDR152W4.062.43E-5GIR2Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein
YDR109C_p3.904.80E-5YDR109C_pPutative kinase
YIL137C3.816.98E-5TMA108Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease
YJR015W_p3.787.87E-5YJR015W_pPutative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene
YMR153C-A_d3.721.00E-4YMR153C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53
YDR162C3.551.94E-4NBP2Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
YDR107C3.522.16E-4TMN2Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments
YKL006W3.502.32E-4RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YOR292C_p3.462.66E-4YOR292C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
YGL021W3.403.39E-4ALK1Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins

GO enrichment analysis for SGTC_1499
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0813.58E-10SGTC_9641287-0107 19.4 μMChemDiv (Drug-like library)515168120.0526316cell wall
0.0706.97E-8SGTC_800159-0065 512.2 μMChemDiv (Drug-like library)15480790.136364
0.0708.46E-8SGTC_33149135860 59.4 μMChembridge (Drug-like library)252524780.109756
0.0673.12E-7SGTC_3191348-1596 45.5 μMChemDiv (Drug-like library)53805050.0666667RSC & ERG11
0.0612.44E-6SGTC_2235salicylic acid 500.0 μMMiscellaneous3380.133333
0.0571.24E-5SGTC_2625euphol 100.0 μMMicrosource (Natural product library)67085380.0744681
0.0543.16E-5SGTC_9453381-0768 11.3 μMChemDiv (Drug-like library)11262360.106667endomembrane recycling
0.0519.99E-5SGTC_10430916-0009 19.3 μMChemDiv (Drug-like library)31011300.0759494
0.0501.11E-4SGTC_23749071832 200.0 μMChembridge (Fragment library)173816980.0857143endomembrane recycling
0.0491.48E-4SGTC_15004488-1866 171.0 μMChemDiv (Drug-like library)29000030.150538RPP1 & pyrimidine depletion
0.0491.65E-4SGTC_80354-0001 70.0 μMChemDiv (Drug-like library)49931520.12987
0.0491.83E-4SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.178082NEO1
0.0482.34E-4SGTC_7531322-0200 289.0 μMChemDiv (Drug-like library)54039070.0963855
0.0473.00E-4SGTC_8650488-0499 301.0 μMChemDiv (Drug-like library)96346510.0810811
0.0473.43E-4SGTC_1834st055981 52.8 μMTimTec (Natural product derivative library)4717190.12987RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15091326-113180 μM0.315789768313ChemDiv (Drug-like library)239.312283.09402
SGTC_11584092-049491.8 μM0.2739731148199ChemDiv (Drug-like library)416.512124.2704
SGTC_3162909965349.47 μM0.26865725237511Chembridge (Drug-like library)300.438423.56802
SGTC_2985902237571.43 μM0.2602744939335Chembridge (Drug-like library)350.454043.68812RPP1 & pyrimidine depletion
SGTC_10472043-304482 μM0.246575669592ChemDiv (Drug-like library)323.385643.66903
SGTC_400327-010319.75 μM0.242424409036ChemDiv (Drug-like library)324.395125.11701
SGTC_680986-028482.83 μM0.242424423278ChemDiv (Drug-like library)324.395125.40801
SGTC_218alverine citrate93.75 μM0.24137921718Miscellaneous473.55861.03748fatty acid desaturase (OLE1)
SGTC_2250alverine citrate130 μM0.24137921718Miscellaneous473.55861.03748
SGTC_2409alverine citrate63.97 μM0.24137921718Miscellaneous473.55861.03748
SGTC_3030908761949.47 μM0.23880625236973Chembridge (Drug-like library)261.359323.08502
SGTC_2970986-003224 μM0.2352942847425ChemDiv (Drug-like library)338.42175.6010160S ribosome export