4491-0275

N-(4-methoxyphenyl)-2-methylbenzo[h]quinolin-1-ium-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1501
Screen concentration 38.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 5104718
SMILES CC1=[NH+]C2=C(C=CC3=CC=CC=C32)C(=C1)NC4=CC=C(C=C4)OC
Standardized SMILES COc1ccc(Nc2cc(C)[nH+]c3c2ccc4ccccc34)cc1
Molecular weight 315.3884
ALogP 5.02
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.97
% growth inhibition (Hom. pool) 4.94


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5104718
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.23||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:BET3(YKR068C)|FD-Score:4.29|P-value:8.74E-6|Clearance:0.51||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:BOS1(YLR078C)|FD-Score:4.41|P-value:5.21E-6|Clearance:0.51||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CEG1(YGL130W)|FD-Score:-3.85|P-value:5.86E-5|Clearance:0||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:EPL1(YFL024C)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.07||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GCD1(YOR260W)|FD-Score:3.79|P-value:7.57E-5|Clearance:0.05||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI10(YGL142C)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.08||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:HTB1(YDR224C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.2||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:NAT2(YGR147C)|FD-Score:-3.83|P-value:6.32E-5|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NBP1(YLR457C)|FD-Score:3.22|P-value:6.36E-4|Clearance:0.02||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.13||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:AFG2(YLR397C)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.23||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:BET3(YKR068C)|FD-Score:4.29|P-value:8.74E-6|Clearance:0.51||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:BOS1(YLR078C)|FD-Score:4.41|P-value:5.21E-6|Clearance:0.51||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CEG1(YGL130W)|FD-Score:-3.85|P-value:5.86E-5|Clearance:0||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:EPL1(YFL024C)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.07||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GCD1(YOR260W)|FD-Score:3.79|P-value:7.57E-5|Clearance:0.05||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI10(YGL142C)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.08||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:HTB1(YDR224C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.2||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:NAT2(YGR147C)|FD-Score:-3.83|P-value:6.32E-5|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NBP1(YLR457C)|FD-Score:3.22|P-value:6.36E-4|Clearance:0.02||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.13||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5104718
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AIM14(YGL160W)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM32(YML050W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARG7(YMR062C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATG29(YPL166W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BUD26(YDR241W_d)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CPR3(YML078W)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:DSE2(YHR143W)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EST3(YIL009C-A)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:IML3(YBR107C)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IMP2'(YIL154C)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC5(YFR038W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEU3(YLR451W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MMS22(YLR320W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MPA43(YNL249C)|FD-Score:4.3|P-value:8.36E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL31(YKL138C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSI1(YBR195C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSO1(YNR049C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NBP2(YDR162C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PDX3(YBR035C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PKH3(YDR466W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RCR2(YDR003W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RTT102(YGR275W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SHE4(YOR035C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHE9(YDR393W)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SPR1(YOR190W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:URH1(YDR400W)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:VHS1(YDR247W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VPS45(YGL095C)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR064W(YBR064W_d)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCL012C(YCL012C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:6.36|P-value:9.95E-11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR114C(YDR114C_p)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YEL057C(YEL057C_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YGR210C(YGR210C_p)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJL150W(YJL150W_d)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:5.43|P-value:2.75E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YOL159C-A(YOL159C-A)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:AAP1(YHR047C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AIM14(YGL160W)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM32(YML050W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARG7(YMR062C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATG29(YPL166W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BUD26(YDR241W_d)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CPR3(YML078W)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:DSE2(YHR143W)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EST3(YIL009C-A)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:IML3(YBR107C)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IMP2'(YIL154C)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC5(YFR038W)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEU3(YLR451W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MMS22(YLR320W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MPA43(YNL249C)|FD-Score:4.3|P-value:8.36E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL31(YKL138C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSI1(YBR195C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSO1(YNR049C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NBP2(YDR162C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PDX3(YBR035C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PKH3(YDR466W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RCR2(YDR003W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RTT102(YGR275W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SHE4(YOR035C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHE9(YDR393W)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SPR1(YOR190W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:URH1(YDR400W)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:VHS1(YDR247W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VPS45(YGL095C)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR064W(YBR064W_d)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCL012C(YCL012C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:6.36|P-value:9.95E-11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR114C(YDR114C_p)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YEL057C(YEL057C_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YGR210C(YGR210C_p)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJL150W(YJL150W_d)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:5.43|P-value:2.75E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YOL159C-A(YOL159C-A)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR078C4.415.21E-60.51BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YKR068C4.298.74E-60.51BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YOR260W3.797.57E-50.05GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YLR397C3.739.41E-50.23AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YKL035W3.512.26E-40.13UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YGL142C3.373.69E-40.08GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YFL024C3.294.99E-40.07EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YLR457C3.226.36E-40.02NBP1Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
YDR224C3.206.91E-40.20HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YPR169W3.000.001360.08JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YOL123W2.920.001780.04HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YDR267C2.880.002010.08CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YJR012C_p2.800.002590.02YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YLR103C2.770.002780.00CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
YDR167W2.770.002810.05TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL118W_p6.369.95E-11YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YKL118W_d5.432.75E-8YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YDR114C_p4.572.39E-6YDR114C_pPutative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
YDR393W4.424.87E-6SHE9Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex
YNL249C4.308.36E-6MPA43Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL095C4.279.70E-6VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YBR035C4.072.39E-5PDX3Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
YDR400W4.032.73E-5URH1Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
YHR143W3.993.26E-5DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YDR466W3.983.42E-5PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
YBR064W_d3.846.15E-5YBR064W_dDubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C
YCL012C3.826.56E-5YCL012CPutative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
YGR210C_p3.758.73E-5YGR210C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YGL160W3.758.82E-5AIM14NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
YPL079W3.701.07E-4RPL21BRibosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1501
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1016.94E-15SGTC_171k064-0041 9.7 μMChemDiv (Drug-like library)533250.155172heme requiring
0.0961.21E-13SGTC_14984478-3555 97.6 μMChemDiv (Drug-like library)34379190.0657895
0.0921.55E-12SGTC_31729105863 49.5 μMChembridge (Drug-like library)23487850.0740741ERG2
0.0766.27E-9SGTC_350k064-0020 19.8 μMChemDiv (Drug-like library)27907050.15873heme requiring
0.0681.50E-7SGTC_1904st060225 78.0 μMTimTec (Natural product derivative library)665950.140625
0.0656.67E-7SGTC_9641287-0107 19.4 μMChemDiv (Drug-like library)515168120.0963855cell wall
0.0631.28E-6SGTC_12161165-0504 9.7 μMChemDiv (Drug-like library)63040040.0769231
0.0612.42E-6SGTC_2689tacrolimus 100.0 μMNIH Clinical Collection235817960.048
0.0612.50E-6SGTC_8730591-4978 488.0 μMChemDiv (Drug-like library)33051450.215385
0.0603.38E-6SGTC_8241304-0008 100.0 μMChemDiv (Drug-like library)7682450.0178571DNA damage response
0.0595.94E-6SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0714286azole & statin
0.0561.49E-5SGTC_12263909-7979 35.4 μMChemDiv (Drug-like library)66155100.115385
0.0561.96E-5SGTC_31889110789 49.5 μMChembridge (Drug-like library)8492170.114286iron homeostasis
0.0543.51E-5SGTC_14801070-0044 41.2 μMChemDiv (Drug-like library)31249870.107692
0.0534.37E-5SGTC_1660st012960 28.1 μMTimTec (Natural product derivative library)242072660.108108iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_5794491-1009110 μM0.450981271041ChemDiv (Drug-like library)284.354444.7571260S ribosome export
SGTC_13953486-01904.35 μM0.3442626748079ChemDiv (Drug-like library)316.353242.38224
SGTC_13833049-003220.7 μM0.333333712864ChemDiv (Drug-like library)291.347084.0261460S ribosome export
SGTC_5171365-0151113 μM0.290909768657ChemDiv (Drug-like library)229.297543.06603
SGTC_3192911036249.47 μM0.27142916649480Chembridge (Drug-like library)358.393223.83914mitochondrial processes
SGTC_2863902873758.44 μM0.2686576460917Chembridge (Drug-like library)322.357823.0514
SGTC_9521185-0122232 μM0.258621678600ChemDiv (Drug-like library)282.360163.8214
SGTC_1547harmine94.2 μM0.2586215280953Miscellaneous212.247182.43812ergosterol biosynthesis
SGTC_2525harmine47.85 μM0.2586215280953Miscellaneous212.247182.43812ergosterol biosynthesis
SGTC_5423381-024489.3 μM0.2575761112843ChemDiv (Drug-like library)360.448863.67603
SGTC_7323970-172752.7 μM0.2567571275699ChemDiv (Drug-like library)416.4263.87115