k297-0037

4-[(4-chlorophenyl)methylamino]-1H-quinazoline-2-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1505
Screen concentration 20.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 4089396
SMILES C1=CC=C2C(=C1)C(=NC(=S)N2)NCC3=CC=C(C=C3)Cl
Standardized SMILES Sc1nc(NCc2ccc(Cl)cc2)c3ccccc3n1
Molecular weight 301.7939
ALogP 4.44
H-bond donor count 2
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.56
% growth inhibition (Hom. pool) 3.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4089396
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:4.63|P-value:1.79E-6|Clearance:0.29||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:ARP2(YDL029W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CCT4(YDL143W)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.1||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC13(YDL220C)|FD-Score:3.98|P-value:3.43E-5|Clearance:0.16||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:DBP2(YNL112W)|FD-Score:4.34|P-value:6.97E-6|Clearance:0.36||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG11(YHR007C)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.12||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:JIP5(YPR169W)|FD-Score:3.1|P-value:9.60E-4|Clearance:0.09||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MAK5(YBR142W)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MSL5(YLR116W)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.14||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:RLP24(YLR009W)|FD-Score:5.49|P-value:2.01E-8|Clearance:0.86||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:SEC23(YPR181C)|FD-Score:3.73|P-value:9.66E-5|Clearance:0.27||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TAF7(YMR227C)|FD-Score:3.36|P-value:3.90E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YGR190C(YGR190C_d)|FD-Score:3.82|P-value:6.71E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:APC1(YNL172W)|FD-Score:4.63|P-value:1.79E-6|Clearance:0.29||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:ARP2(YDL029W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CCT4(YDL143W)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.1||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC13(YDL220C)|FD-Score:3.98|P-value:3.43E-5|Clearance:0.16||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:DBP2(YNL112W)|FD-Score:4.34|P-value:6.97E-6|Clearance:0.36||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG11(YHR007C)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.12||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:JIP5(YPR169W)|FD-Score:3.1|P-value:9.60E-4|Clearance:0.09||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MAK5(YBR142W)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MSL5(YLR116W)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.14||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:RLP24(YLR009W)|FD-Score:5.49|P-value:2.01E-8|Clearance:0.86||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:SEC23(YPR181C)|FD-Score:3.73|P-value:9.66E-5|Clearance:0.27||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TAF7(YMR227C)|FD-Score:3.36|P-value:3.90E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YGR190C(YGR190C_d)|FD-Score:3.82|P-value:6.71E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4089396
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ALG5(YPL227C)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARC18(YLR370C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARD1(YHR013C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARR3(YPR201W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:BDH1(YAL060W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BNI4(YNL233W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BXI1(YNL305C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CIT1(YNR001C)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CRS5(YOR031W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DAK2(YFL053W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DSE2(YHR143W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:FET4(YMR319C)|FD-Score:6.45|P-value:5.71E-11||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FUM1(YPL262W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GSH2(YOL049W)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:GTT2(YLL060C)|FD-Score:-5.43|P-value:2.86E-8||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HNT1(YDL125C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HOR7(YMR251W-A)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HXT10(YFL011W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:KHA1(YJL094C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MFA1(YDR461W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MFT1(YML062C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MKT1(YNL085W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRP51(YPL118W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:NUP133(YKR082W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OMA1(YKR087C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PFK1(YGR240C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGC1(YPL206C)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PRO2(YOR323C)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUS4(YNL292W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RPH1(YER169W)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:SAP1(YER047C)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SGA1(YIL099W)|FD-Score:-4.98|P-value:3.13E-7||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SIN3(YOL004W)|FD-Score:6.91|P-value:2.40E-12||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SNF3(YDL194W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SOM1(YEL059C-A)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPE4(YLR146C)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSE1(YPL106C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:8.13|P-value:2.11E-16||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:TCM62(YBR044C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TGL3(YMR313C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TRP1(YDR007W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VAC14(YLR386W)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAN1(YML115C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA16(YHR026W)|FD-Score:7.41|P-value:6.15E-14||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA4(YOR332W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:XYL2(YLR070C)|FD-Score:6.64|P-value:1.56E-11||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR099C(YCR099C_p)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function Gene:YDL177C(YDL177C_p)|FD-Score:-4.86|P-value:5.79E-7||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR095C(YDR095C_d)|FD-Score:-4.36|P-value:6.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL034W(YFL034W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YKE4(YIL023C)|FD-Score:-4.37|P-value:6.31E-6||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL133C(YKL133C_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLL059C(YLL059C_d)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR012C(YLR012C_p)|FD-Score:4.98|P-value:3.12E-7||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR177W(YLR177W_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YMR075C-A(YMR075C-A_d)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL043C(YNL043C_d)|FD-Score:-4.84|P-value:6.49E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL198C(YNL198C_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR345C(YOR345C_d)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPK1(YKL126W)|FD-Score:5.02|P-value:2.58E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR027C(YPR027C_p)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function Gene:ACE2(YLR131C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ALG5(YPL227C)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARC18(YLR370C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARD1(YHR013C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARR3(YPR201W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:BDH1(YAL060W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BNI4(YNL233W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BXI1(YNL305C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CIT1(YNR001C)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CRS5(YOR031W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DAK2(YFL053W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DSE2(YHR143W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:FET4(YMR319C)|FD-Score:6.45|P-value:5.71E-11||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FUM1(YPL262W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GSH2(YOL049W)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:GTT2(YLL060C)|FD-Score:-5.43|P-value:2.86E-8||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HNT1(YDL125C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HOR7(YMR251W-A)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HXT10(YFL011W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:KHA1(YJL094C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MFA1(YDR461W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MFT1(YML062C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MKT1(YNL085W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRP51(YPL118W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:NUP133(YKR082W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OMA1(YKR087C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PFK1(YGR240C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGC1(YPL206C)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PRO2(YOR323C)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUS4(YNL292W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RPH1(YER169W)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:SAP1(YER047C)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SGA1(YIL099W)|FD-Score:-4.98|P-value:3.13E-7||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SIN3(YOL004W)|FD-Score:6.91|P-value:2.40E-12||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SNF3(YDL194W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SOM1(YEL059C-A)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPE4(YLR146C)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSE1(YPL106C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:8.13|P-value:2.11E-16||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:TCM62(YBR044C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TGL3(YMR313C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TRP1(YDR007W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VAC14(YLR386W)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAN1(YML115C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA16(YHR026W)|FD-Score:7.41|P-value:6.15E-14||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA4(YOR332W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:XYL2(YLR070C)|FD-Score:6.64|P-value:1.56E-11||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR099C(YCR099C_p)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function Gene:YDL177C(YDL177C_p)|FD-Score:-4.86|P-value:5.79E-7||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR095C(YDR095C_d)|FD-Score:-4.36|P-value:6.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL034W(YFL034W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YKE4(YIL023C)|FD-Score:-4.37|P-value:6.31E-6||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL133C(YKL133C_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLL059C(YLL059C_d)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR012C(YLR012C_p)|FD-Score:4.98|P-value:3.12E-7||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR177W(YLR177W_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YMR075C-A(YMR075C-A_d)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL043C(YNL043C_d)|FD-Score:-4.84|P-value:6.49E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL198C(YNL198C_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR345C(YOR345C_d)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPK1(YKL126W)|FD-Score:5.02|P-value:2.58E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR027C(YPR027C_p)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR009W5.492.01E-80.86RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YNL172W4.631.79E-60.29APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YNL112W4.346.97E-60.36DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YDL220C3.983.43E-50.16CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YGR190C_d3.826.71E-50.09YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YPR181C3.739.66E-50.27SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YDL143W3.462.70E-40.10CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YMR227C3.363.90E-40.00TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YLR116W3.363.94E-40.14MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YHR007C3.226.37E-40.12ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YPR169W3.109.60E-40.09JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YLR249W3.010.001310.07YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YGR119C2.940.001650.06NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YOR217W2.880.001970.16RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YIL142W2.720.003270.01CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNR031C8.132.11E-16SSK2MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
YHR026W7.416.15E-14VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YOL004W6.912.40E-12SIN3Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
YLR070C6.641.56E-11XYL2Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
YMR319C6.455.71E-11FET4Low-affinity Fe(II) transporter of the plasma membrane
YOR323C6.281.72E-10PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YKL126W5.022.58E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR012C_p4.983.12E-7YLR012C_pPutative protein of unknown function; YLR012C is not an essential gene
YNR029C_p4.885.35E-7YNR029C_pPutative protein of unknown function, deletion confers reduced fitness in saline
YPL227C4.562.57E-6ALG5UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum
YHR013C4.376.09E-6ARD1Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress
YHR143W4.356.85E-6DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YNL085W4.298.90E-6MKT1Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress
YMR075C-A_d4.221.21E-5YMR075C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W
YPL118W4.211.26E-5MRP51Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences

GO enrichment analysis for SGTC_1505
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0977.50E-14SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.0266667
0.0961.84E-13SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.0857143
0.0862.92E-11SGTC_15110906-3109 28.5 μMChemDiv (Drug-like library)28821800.0555556excess fatty acid
0.0863.09E-11SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.134328iron homeostasis
0.0863.47E-11SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.134328
0.0831.52E-10SGTC_772k072-0172 35.3 μMChemDiv (Drug-like library)58619800.1
0.0813.81E-10SGTC_18845633444 25.0 μMMiscellaneous57242390.241935redox potentiating
0.0808.05E-10SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.111111
0.0772.68E-9SGTC_251melphalan 456.5 μMMiscellaneous4606120.111111DNA damage response
0.0764.88E-9SGTC_1076itraconazole 331.0 nMMiscellaneous37930.0825688
0.0751.01E-8SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)53280140.372549
0.0741.43E-8SGTC_2074185-0010 4.5 μMChemDiv (Drug-like library)37617530.0212766endomembrane recycling
0.0732.34E-8SGTC_2657nystatin 74.4 μMMiscellaneous114687070.0178571
0.0723.27E-8SGTC_20835306046 65.9 μMChembridge (Fragment library)5398370.031746
0.0715.63E-8SGTC_9811488-1094 54.9 μMChemDiv (Drug-like library)34495220.047619

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1037k284-109178.7 μM0.7619053334711ChemDiv (Drug-like library)285.3392833.98522
SGTC_282k297-003665.7 μM0.755061378ChemDiv (Drug-like library)301.793884.44421
SGTC_1467k284-1082293 μM0.5957453334705ChemDiv (Drug-like library)273.376543.5222
SGTC_1468k284-1086134 μM0.52830215996457ChemDiv (Drug-like library)360.453842.80634DNA intercalators
SGTC_1504k297-003330 μM0.475414388117ChemDiv (Drug-like library)413.966764.69123
SGTC_14023909-767335.9 μM0.3725495328014ChemDiv (Drug-like library)269.728883.98313
SGTC_232clozapine119 μM0.32818Miscellaneous326.823263.47614NEO1
SGTC_443clozapine122 μM0.32818Miscellaneous326.823263.47614NEO1-PIK1
SGTC_900870-08634.61 μM0.2622951738164ChemDiv (Drug-like library)311.762263.53812
SGTC_797k781-0471270 μM0.26229523738129ChemDiv (Drug-like library)306.701143.4125RPP1 & pyrimidine depletion
SGTC_20375169046200 μM0.255718022Chembridge (Fragment library)204.22518-0.0923460S ribosome export