0259-0544

2-[(2,4-dichlorophenoxy)methyl]-1H-benzimidazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1512
Screen concentration 12.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 18476
SMILES C1=CC=C2C(=C1)NC(=N2)COC3=C(C=C(C=C3)Cl)Cl
Standardized SMILES Clc1ccc(OCc2nc3ccccc3[nH]2)c(Cl)c1
Molecular weight 293.148
ALogP 4.43
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.45
% growth inhibition (Hom. pool) 1.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 18476
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:9.15|P-value:2.84E-20|Clearance:3.61||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CCA1(YER168C)|FD-Score:-3.75|P-value:8.76E-5|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCL1(YPR025C)|FD-Score:3.77|P-value:8.24E-5|Clearance:0.04||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:COG2(YGR120C)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.08||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ESF2(YNR054C)|FD-Score:3.72|P-value:9.78E-5|Clearance:0.17||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:MRD1(YPR112C)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:PGA1(YNL158W)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRP38(YGR075C)|FD-Score:4.03|P-value:2.79E-5|Clearance:0.2||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPF1(YHR088W)|FD-Score:-3.11|P-value:9.42E-4|Clearance:0||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL28(YGL103W)|FD-Score:4.62|P-value:1.90E-6|Clearance:0.59||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RSC4(YKR008W)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SCL1(YGL011C)|FD-Score:3.32|P-value:4.43E-4|Clearance:0.12||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC13(YLR208W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC3(YER008C)|FD-Score:5.54|P-value:1.52E-8|Clearance:0.92||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.06||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TCP1(YDR212W)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.04||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFA1(YKL028W)|FD-Score:-4.49|P-value:3.54E-6|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:YKL083W(YKL083W_d)|FD-Score:-3.53|P-value:2.04E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YRB1(YDR002W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.2||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:ARP2(YDL029W)|FD-Score:9.15|P-value:2.84E-20|Clearance:3.61||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CCA1(YER168C)|FD-Score:-3.75|P-value:8.76E-5|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCL1(YPR025C)|FD-Score:3.77|P-value:8.24E-5|Clearance:0.04||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:COG2(YGR120C)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.08||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ESF2(YNR054C)|FD-Score:3.72|P-value:9.78E-5|Clearance:0.17||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:MRD1(YPR112C)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:PGA1(YNL158W)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRP38(YGR075C)|FD-Score:4.03|P-value:2.79E-5|Clearance:0.2||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPF1(YHR088W)|FD-Score:-3.11|P-value:9.42E-4|Clearance:0||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL28(YGL103W)|FD-Score:4.62|P-value:1.90E-6|Clearance:0.59||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RSC4(YKR008W)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SCL1(YGL011C)|FD-Score:3.32|P-value:4.43E-4|Clearance:0.12||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC13(YLR208W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC3(YER008C)|FD-Score:5.54|P-value:1.52E-8|Clearance:0.92||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.06||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TCP1(YDR212W)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.04||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFA1(YKL028W)|FD-Score:-4.49|P-value:3.54E-6|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:YKL083W(YKL083W_d)|FD-Score:-3.53|P-value:2.04E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YRB1(YDR002W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.2||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 18476
Download HOP data (tab-delimited text)  (excel)
Gene:ADP1(YCR011C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AEP3(YPL005W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM44(YPL158C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:AIM6(YDL237W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALG5(YPL227C)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APM1(YPL259C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ASI2(YNL159C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:BAS1(YKR099W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BNA7(YDR428C)|FD-Score:5.09|P-value:1.75E-7||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BRE1(YDL074C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD30(YDL151C_d)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL2(YML111W)|FD-Score:-4.47|P-value:3.99E-6||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:COA3(YJL062W-A)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COG5(YNL051W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS4(YGR155W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAT1(YML113W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DBF2(YGR092W)|FD-Score:-3.99|P-value:3.37E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DPP1(YDR284C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:GAC1(YOR178C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GIC1(YHR061C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GIT1(YCR098C)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPM3(YOL056W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HIT1(YJR055W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IDP3(YNL009W)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:KEX1(YGL203C)|FD-Score:5.7|P-value:5.88E-9||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:MCH1(YDL054C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM36(YPR083W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MFM1(YPL060W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MIR1(YJR077C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MNN11(YJL183W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNN2(YBR015C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP7(YNL005C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST1(YKL194C)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NDT80(YHR124W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NOP13(YNL175C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NUP60(YAR002W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:OAF1(YAL051W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OSH2(YDL019C)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PET54(YGR222W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX13(YLR191W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PKH1(YDR490C)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PKR1(YMR123W)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POX1(YGL205W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PSD2(YGR170W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUS4(YNL292W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAM1(YDL090C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RBG2(YGR173W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:REV7(YIL139C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGS2(YOR107W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RIM4(YHL024W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL34A(YER056C-A)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPN13(YLR421C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPP1A(YDL081C)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:SAM37(YMR060C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAS4(YDR181C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SNF4(YGL115W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPT8(YLR055C)|FD-Score:-3.82|P-value:6.80E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRF1(YDL133W)|FD-Score:4.32|P-value:7.73E-6||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSK2(YNR031C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:STF2(YGR008C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:STI1(YOR027W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:THI73(YLR004C)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:THP3(YPR045C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:VTS1(YOR359W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Putative protein of unknown function Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YCL002C(YCL002C_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR015C(YCR015C_p)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:4|P-value:3.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL177C(YDL177C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR445C(YDR445C_d)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL118C(YGL118C_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR015C(YGR015C_p)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YIR044C(YIR044C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL022W(YJL022W_d)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR061W(YJR061W_p)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YJU3(YKL094W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL031W(YKL031W_d)|FD-Score:-4.8|P-value:7.98E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL133C(YKL133C_p)|FD-Score:-4.04|P-value:2.63E-5||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YML047W-A(YML047W-A_d)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR245W(YMR245W_d)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL013W-A(YOL013W-A_p)|FD-Score:5.2|P-value:9.73E-8||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL131W(YOL131W_p)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Putative protein of unknown function Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR170W(YOR170W_d)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR225W(YOR225W_d)|FD-Score:5.41|P-value:3.13E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADP1(YCR011C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AEP3(YPL005W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM44(YPL158C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:AIM6(YDL237W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALG5(YPL227C)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APM1(YPL259C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ASI2(YNL159C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:BAS1(YKR099W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BNA7(YDR428C)|FD-Score:5.09|P-value:1.75E-7||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BRE1(YDL074C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD30(YDL151C_d)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL2(YML111W)|FD-Score:-4.47|P-value:3.99E-6||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:COA3(YJL062W-A)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COG5(YNL051W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS4(YGR155W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAT1(YML113W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DBF2(YGR092W)|FD-Score:-3.99|P-value:3.37E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DPP1(YDR284C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:GAC1(YOR178C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GIC1(YHR061C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GIT1(YCR098C)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPM3(YOL056W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HIT1(YJR055W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IDP3(YNL009W)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:KEX1(YGL203C)|FD-Score:5.7|P-value:5.88E-9||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:MCH1(YDL054C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM36(YPR083W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MFM1(YPL060W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MIR1(YJR077C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MNN11(YJL183W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNN2(YBR015C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP7(YNL005C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST1(YKL194C)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NDT80(YHR124W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NOP13(YNL175C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NUP60(YAR002W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:OAF1(YAL051W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OSH2(YDL019C)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PET54(YGR222W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX13(YLR191W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PKH1(YDR490C)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PKR1(YMR123W)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POX1(YGL205W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PSD2(YGR170W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUS4(YNL292W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAM1(YDL090C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RBG2(YGR173W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:REV7(YIL139C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGS2(YOR107W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RIM4(YHL024W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL34A(YER056C-A)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPN13(YLR421C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPP1A(YDL081C)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:SAM37(YMR060C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAS4(YDR181C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SNF4(YGL115W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPT8(YLR055C)|FD-Score:-3.82|P-value:6.80E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRF1(YDL133W)|FD-Score:4.32|P-value:7.73E-6||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSK2(YNR031C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:STF2(YGR008C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:STI1(YOR027W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:THI73(YLR004C)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:THP3(YPR045C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:VTS1(YOR359W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Putative protein of unknown function Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YCL002C(YCL002C_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR015C(YCR015C_p)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:4|P-value:3.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL177C(YDL177C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR445C(YDR445C_d)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL118C(YGL118C_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR015C(YGR015C_p)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YIR044C(YIR044C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL022W(YJL022W_d)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR061W(YJR061W_p)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YJU3(YKL094W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL031W(YKL031W_d)|FD-Score:-4.8|P-value:7.98E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL133C(YKL133C_p)|FD-Score:-4.04|P-value:2.63E-5||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YML047W-A(YML047W-A_d)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR245W(YMR245W_d)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL013W-A(YOL013W-A_p)|FD-Score:5.2|P-value:9.73E-8||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL131W(YOL131W_p)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Putative protein of unknown function Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR170W(YOR170W_d)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR225W(YOR225W_d)|FD-Score:5.41|P-value:3.13E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL029W9.152.84E-203.61ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YER008C5.541.52E-80.92SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YGL103W4.621.90E-60.59RPL28Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance
YGR075C4.032.79E-50.20PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YGL093W3.836.36E-50.06SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YPR025C3.778.24E-50.04CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
YNR054C3.729.78E-50.17ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YDR002W3.561.86E-40.20YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YDR212W3.363.91E-40.04TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YGL011C3.324.43E-40.12SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YGR120C3.216.72E-40.08COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR208W3.138.82E-40.08SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YCR093W3.050.001150.07CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YIR022W2.980.001430.18SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YPR104C2.810.002510.00FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL203C5.705.88E-9KEX1Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
YPL227C5.601.05E-8ALG5UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum
YDL054C5.442.60E-8MCH1Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
YOR225W_d5.413.13E-8YOR225W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL013W-A_p5.209.73E-8YOL013W-A_pPutative protein of unknown function; identified by SAGE
YDR428C5.091.75E-7BNA7Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
YDL081C4.592.18E-6RPP1ARibosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
YDL019C4.347.20E-6OSH2Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YDL133W4.327.73E-6SRF1Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
YDR284C4.131.85E-5DPP1Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
YGR015C_p4.092.17E-5YGR015C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YOL131W_p4.072.39E-5YOL131W_pPutative protein of unknown function
YDL090C4.022.93E-5RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
YDL074C4.013.08E-5BRE1E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control
YCR049C_d4.003.23E-5YCR049C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1512
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1376.05E-26SGTC_14314049-0260 9.1 μMChemDiv (Drug-like library)38548410.132353
0.1025.01E-15SGTC_2419paclitaxel 166.5 μMMiscellaneous46660.0508475
0.0952.26E-13SGTC_2537larixol 68.9 μMMicrosource (Natural product library)67087590.025641
0.0823.37E-10SGTC_14123909-8563 178.0 μMChemDiv (Drug-like library)237675300.11236
0.0813.84E-10SGTC_13762910-0861 41.3 μMChemDiv (Drug-like library)11128590.12987
0.0791.02E-9SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.164179
0.0791.32E-9SGTC_23387999974 200.0 μMChembridge (Fragment library)3159250.0588235
0.0722.48E-8SGTC_24045161160 200.0 μMMiscellaneous28315430.140845
0.0723.16E-8SGTC_25422-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 100.0 μMMicrosource (Natural product library)5024745NA
0.0691.10E-7SGTC_33379144342 33.5 μMChembridge (Drug-like library)176829490.08
0.0681.55E-7SGTC_15130337-0559 19.0 μMChemDiv (Drug-like library)7750730.157895
0.0672.62E-7SGTC_15666,7-dimethoxy-4-ethylcoumarin 85.4 μMTimTec (Pure natural product library)6887610.0967742
0.0654.97E-7SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0694444
0.0648.44E-7SGTC_1563conessine 56.1 μMTimTec (Pure natural product library)4410820.0243902
0.0631.19E-6SGTC_1804st050076 49.1 μMTimTec (Natural product derivative library)38959760.131579

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1974544-004341.56 μM0.444444896523ChemDiv (Drug-like library)322.189224.30713endomembrane recycling
SGTC_287k060-003929.6 μM0.4259265861974ChemDiv (Drug-like library)305.1624.83423
SGTC_660109-018742.4 μM0.422222759417ChemDiv (Drug-like library)228.676963.93511
SGTC_6480375-047333.3 μM0.3958331713139ChemDiv (Drug-like library)230.348585.0111
SGTC_3295912236253.57 μM0.3770494730473Chembridge (Drug-like library)349.214564.14614
SGTC_3119912518349.47 μM0.36538522695334Chembridge (Drug-like library)291.370224.38913
SGTC_655k060-001242 μM0.3620695994525ChemDiv (Drug-like library)288.7074034.37524
SGTC_6681682-049962.6 μM0.357143682214ChemDiv (Drug-like library)324.20184.10612
SGTC_1704197-0004226 μM0.344828218692ChemDiv (Drug-like library)317.793283.72923
SGTC_3303912561840.58 μM0.34482822695467Chembridge (Drug-like library)332.422144.32714