3460-0031

N-[(8-hydroxyquinolin-7-yl)-(2-methoxyphenyl)methyl]butanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1515
Screen concentration 42.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 2876596
SMILES CCCC(=O)NC(C1=C(C2=C(C=CC=N2)C=C1)O)C3=CC=CC=C3OC
Standardized SMILES CCCC(=O)NC(c1ccc2cccnc2c1O)c3ccccc3OC
Molecular weight 350.411
ALogP 3.62
H-bond donor count 2
H-bond acceptor count 4
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.82
% growth inhibition (Hom. pool) -1.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2876596
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:-3.27|P-value:5.40E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP2(YDL029W)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.06||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CEP3(YMR168C)|FD-Score:7.48|P-value:3.74E-14|Clearance:1.57||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CLP1(YOR250C)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.15||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:CWC23(YGL128C)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.07||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:DAD1(YDR016C)|FD-Score:-3.25|P-value:5.75E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG12(YMR208W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.09||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.15||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HRR25(YPL204W)|FD-Score:-5.13|P-value:1.47E-7|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MCM3(YEL032W)|FD-Score:5.96|P-value:1.27E-9|Clearance:1.57||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NAF1(YNL124W)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.01||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:POL3(YDL102W)|FD-Score:5.85|P-value:2.53E-9|Clearance:1.57||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE10(YOR362C)|FD-Score:5.72|P-value:5.18E-9|Clearance:1.57||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RRN7(YJL025W)|FD-Score:6.11|P-value:4.88E-10|Clearance:1.57||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SAM35(YHR083W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.09||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SEC26(YDR238C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.07||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SNU56(YDR240C)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.02||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRB6(YBR253W)|FD-Score:3.26|P-value:5.48E-4|Clearance:0.04||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:UTP10(YJL109C)|FD-Score:-3.17|P-value:7.62E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:UTP14(YML093W)|FD-Score:3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VHT1(YGR065C)|FD-Score:4|P-value:3.16E-5|Clearance:0.31||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YLL037W(YLL037W_d)|FD-Score:-3.65|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:ARC40(YBR234C)|FD-Score:-3.27|P-value:5.40E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP2(YDL029W)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.06||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CEP3(YMR168C)|FD-Score:7.48|P-value:3.74E-14|Clearance:1.57||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CLP1(YOR250C)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.15||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:CWC23(YGL128C)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.07||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:DAD1(YDR016C)|FD-Score:-3.25|P-value:5.75E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG12(YMR208W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.09||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.15||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HRR25(YPL204W)|FD-Score:-5.13|P-value:1.47E-7|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MCM3(YEL032W)|FD-Score:5.96|P-value:1.27E-9|Clearance:1.57||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NAF1(YNL124W)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.01||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:POL3(YDL102W)|FD-Score:5.85|P-value:2.53E-9|Clearance:1.57||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE10(YOR362C)|FD-Score:5.72|P-value:5.18E-9|Clearance:1.57||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RRN7(YJL025W)|FD-Score:6.11|P-value:4.88E-10|Clearance:1.57||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SAM35(YHR083W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.09||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SEC26(YDR238C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.07||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SNU56(YDR240C)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.02||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRB6(YBR253W)|FD-Score:3.26|P-value:5.48E-4|Clearance:0.04||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:UTP10(YJL109C)|FD-Score:-3.17|P-value:7.62E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:UTP14(YML093W)|FD-Score:3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VHT1(YGR065C)|FD-Score:4|P-value:3.16E-5|Clearance:0.31||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YLL037W(YLL037W_d)|FD-Score:-3.65|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2876596
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AFT1(YGL071W)|FD-Score:4.35|P-value:6.74E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM44(YPL158C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:AQY2(YLL052C)|FD-Score:-4.01|P-value:3.01E-5||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARG82(YDR173C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG12(YBR217W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:CCW12(YLR110C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:COX7(YMR256C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CWP2(YKL096W-A)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:EFM1(YHL039W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HNT1(YDL125C)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSP30(YCR021C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:MAC1(YMR021C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM34(YGL219C)|FD-Score:4.28|P-value:9.28E-6||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP13(YGR084C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL24(YMR193W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL37(YBR268W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAB6(YML117W)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NUP2(YLR335W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:PMU1(YKL128C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:RAD34(YDR314C)|FD-Score:4.79|P-value:8.48E-7||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RAX1(YOR301W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RPN4(YDL020C)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP155(YFR040W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SHS1(YDL225W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SNT1(YCR033W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:STR3(YGL184C)|FD-Score:-4.9|P-value:4.89E-7||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:TDA8(YAL064C-A_p)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TIM18(YOR297C)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TMA64(YDR117C)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPM2(YIL138C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:UBX5(YDR330W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR015C(YDR015C_d)|FD-Score:-4.34|P-value:7.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR271C(YDR271C_d)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YER084W(YER084W_p)|FD-Score:-4.84|P-value:6.56E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFR020W(YFR020W_p)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL041C(YGL041C_d)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative protein of unknown function Gene:YGL118C(YGL118C_d)|FD-Score:11.1|P-value:3.89E-29||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR153W(YGR153W_p)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Putative protein of unknown function Gene:YIL102C(YIL102C_p)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Putative protein of unknown function Gene:YJL022W(YJL022W_d)|FD-Score:-5|P-value:2.89E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YMR166C(YMR166C_p)|FD-Score:5.2|P-value:1.00E-7||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR206W(YMR206W_p)|FD-Score:4.84|P-value:6.55E-7||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YMR262W(YMR262W_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR027C(YPR027C_p)|FD-Score:5.4|P-value:3.36E-8||SGD DESC:Putative protein of unknown function Gene:YPR170C(YPR170C_d)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPR197C(YPR197C_d)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ABZ2(YMR289W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AFT1(YGL071W)|FD-Score:4.35|P-value:6.74E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM44(YPL158C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:AQY2(YLL052C)|FD-Score:-4.01|P-value:3.01E-5||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARG82(YDR173C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG12(YBR217W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:CCW12(YLR110C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:COX7(YMR256C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CWP2(YKL096W-A)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:EFM1(YHL039W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HNT1(YDL125C)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSP30(YCR021C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:MAC1(YMR021C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM34(YGL219C)|FD-Score:4.28|P-value:9.28E-6||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP13(YGR084C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL24(YMR193W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL37(YBR268W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAB6(YML117W)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NUP2(YLR335W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:PMU1(YKL128C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:RAD34(YDR314C)|FD-Score:4.79|P-value:8.48E-7||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RAX1(YOR301W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RPN4(YDL020C)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP155(YFR040W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SHS1(YDL225W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SNT1(YCR033W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:STR3(YGL184C)|FD-Score:-4.9|P-value:4.89E-7||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:TDA8(YAL064C-A_p)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TIM18(YOR297C)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TMA64(YDR117C)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPM2(YIL138C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:UBX5(YDR330W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR015C(YDR015C_d)|FD-Score:-4.34|P-value:7.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR271C(YDR271C_d)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YER084W(YER084W_p)|FD-Score:-4.84|P-value:6.56E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFR020W(YFR020W_p)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL041C(YGL041C_d)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative protein of unknown function Gene:YGL118C(YGL118C_d)|FD-Score:11.1|P-value:3.89E-29||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR153W(YGR153W_p)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Putative protein of unknown function Gene:YIL102C(YIL102C_p)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Putative protein of unknown function Gene:YJL022W(YJL022W_d)|FD-Score:-5|P-value:2.89E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YMR166C(YMR166C_p)|FD-Score:5.2|P-value:1.00E-7||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR206W(YMR206W_p)|FD-Score:4.84|P-value:6.55E-7||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YMR262W(YMR262W_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR027C(YPR027C_p)|FD-Score:5.4|P-value:3.36E-8||SGD DESC:Putative protein of unknown function Gene:YPR170C(YPR170C_d)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPR197C(YPR197C_d)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR168C7.483.74E-141.57CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YJL025W6.114.88E-101.57RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YEL032W5.961.27E-91.57MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YDL102W5.852.53E-91.57POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YOR362C5.725.18E-91.57PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YOR250C4.151.64E-50.15CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YGR065C4.003.16E-50.31VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YJL085W3.691.11E-40.15EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YMR208W3.541.99E-40.09ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YNL124W3.462.75E-40.01NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YHR083W3.452.81E-40.09SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YML093W3.363.94E-40.00UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDR240C3.354.01E-40.02SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YDL029W3.334.36E-40.06ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YBR253W3.265.48E-40.04SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL118C_d11.103.89E-29YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR027C_p5.403.36E-8YPR027C_pPutative protein of unknown function
YMR166C_p5.201.00E-7YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YMR289W5.161.23E-7ABZ2Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
YDR271C_d5.042.37E-7YDR271C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
YPR170C_d4.993.00E-7YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YOR133W4.983.13E-7EFT1Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
YMR206W_p4.846.55E-7YMR206W_pPutative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
YDR314C4.798.48E-7RAD34Protein involved in nucleotide excision repair (NER); homologous to RAD4
YDL223C4.552.65E-6HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YGL071W4.356.74E-6AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YGL219C4.289.28E-6MDM34Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
YHL039W4.221.20E-5EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YIL102C_p4.171.53E-5YIL102C_pPutative protein of unknown function
YDL100C4.141.77E-5GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1515
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4286.02E-262SGTC_264promethazine 12.5 mMMiscellaneous60140.111111DNA intercalators
0.3566.09E-176SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.155844DNA intercalators
0.3161.78E-136SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.146341DNA intercalators
0.3122.93E-133SGTC_1519esterone 74.0 μMTimTec (Pure natural product library)6980.0823529DNA intercalators
0.3103.82E-132SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.123457DNA intercalators
0.2734.62E-101SGTC_1468k284-1086 134.0 μMChemDiv (Drug-like library)159964570.104651DNA intercalators
0.2482.92E-83SGTC_14873970-0795 2.8 μMChemDiv (Drug-like library)37651030.0641026DNA intercalators
0.1902.67E-49SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0769231DNA intercalators
0.1832.08E-45SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.108108DNA intercalators
0.1633.27E-36SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.113636DNA intercalators
0.1611.18E-35SGTC_257daunorubicin 18.5 μMMiscellaneous303230.165049anthracycline transcription coupled DNA repair
0.1596.91E-35SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.16092
0.1449.07E-29SGTC_1260417-1624 672.5 μMChemDiv (Drug-like library)419120.103896DNA intercalators
0.1431.56E-28SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.208955DNA intercalators
0.1361.27E-25SGTC_1529apigenin 74.0 μMTimTec (Pure natural product library)52804430.0779221

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_5783460-005192 μM0.5645162876744ChemDiv (Drug-like library)320.3853.66623
SGTC_2914796821845.32 μM0.4117652977111Chembridge (Drug-like library)345.3696433.83325
SGTC_1666st01482929 μM0.3037973858230TimTec (Natural product derivative library)431.450773.8228calcium & mitochondrial duress
SGTC_1090132-003633.7 μM0.29850767274ChemDiv (Drug-like library)293.31663.26423
SGTC_9292805-0021398 μM0.2962963098024ChemDiv (Drug-like library)369.39443.07216
SGTC_3157909927449.47 μM0.26315817217662Chembridge (Drug-like library)339.43143.29104
SGTC_20945332512191.67 μM0.261538755774Chembridge (Fragment library)215.247821.59512
SGTC_2000st07525436.4 μM0.26136417234175TimTec (Natural product derivative library)549.594883.7210
SGTC_3016908086649.47 μM0.25925917066299Chembridge (Drug-like library)331.392781.94116
SGTC_21695-(ethoxymethyl)quinolin-8-ol49.51 μM0.253731279621Chembridge (Fragment library)203.237121.92713