deoxycholic acid methyl ester

methyl (4R)-4-[(3R,5R,8R,9S,10S,12S,13R,14S,17R)-3,12-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoate

A bile salt which induces apoptosis.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1517
Screen concentration 49.2 μM
Source TimTec (Pure natural product library)
PubChem CID 229346
SMILES CC(CCC(=O)OC)C1CCC2C1(C(CC3C2CCC4C3(CCC(C4)O)C)O)C
Standardized SMILES COC(=O)CCC(C)C1CCC2C3CCC4CC(O)CCC4(C)C3CC(O)C12C
Molecular weight 406.5986
ALogP 4.31
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.89
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 229346
Download HIP data (tab-delimited text)  (excel)
Gene:CCA1(YER168C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.04||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT4(YDL143W)|FD-Score:6.56|P-value:2.64E-11|Clearance:2.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC3(YLR314C)|FD-Score:-4.54|P-value:2.78E-6|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:MED11(YMR112C)|FD-Score:3.21|P-value:6.61E-4|Clearance:0.11||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:SEC12(YNR026C)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:TRE2(YOR256C)|FD-Score:-3.35|P-value:4.09E-4|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:VTI1(YMR197C)|FD-Score:5.32|P-value:5.26E-8|Clearance:2.09||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YEF3(YLR249W)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.02||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YGL074C(YGL074C_d)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:CCA1(YER168C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.04||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT4(YDL143W)|FD-Score:6.56|P-value:2.64E-11|Clearance:2.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC3(YLR314C)|FD-Score:-4.54|P-value:2.78E-6|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:MED11(YMR112C)|FD-Score:3.21|P-value:6.61E-4|Clearance:0.11||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:SEC12(YNR026C)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:TRE2(YOR256C)|FD-Score:-3.35|P-value:4.09E-4|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:VTI1(YMR197C)|FD-Score:5.32|P-value:5.26E-8|Clearance:2.09||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YEF3(YLR249W)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.02||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YGL074C(YGL074C_d)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 229346
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AQY2(YLL052C)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ATO3(YDR384C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:ATP18(YML081C-A)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms Gene:BAP2(YBR068C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BIM1(YER016W)|FD-Score:-8.33|P-value:3.99E-17||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BUD23(YCR047C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CDC73(YLR418C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-3.87|P-value:5.38E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CUR1(YPR158W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DBP7(YKR024C)|FD-Score:-4.58|P-value:2.37E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DEG1(YFL001W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DSF2(YBR007C_p)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG1(YFR044C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELP3(YPL086C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENO1(YGR254W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:EST2(YLR318W)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FLO8(YER109C)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FSF1(YOR271C_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:FYV12(YOR183W_p)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GIT1(YCR098C)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GTT2(YLL060C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HVG1(YER039C)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IOC4(YMR044W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC13(YOR235W_d)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:KEL1(YHR158C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LDB16(YCL005W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEM3(YNL323W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAL13(YGR288W)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MNS1(YJR131W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MRPL20(YKR085C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA1(YOR066W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSB2(YGR014W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSS51(YLR203C)|FD-Score:-3.81|P-value:6.96E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MTG1(YMR097C)|FD-Score:-17.3|P-value:1.95E-67||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NPC2(YDL046W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:PAC10(YGR078C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAT1(YCR077C)|FD-Score:-6.94|P-value:1.91E-12||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PCK1(YKR097W)|FD-Score:4.5|P-value:3.40E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PGM1(YKL127W)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:RCF2(YNR018W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RIM4(YHL024W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL26A(YLR344W)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTF1(YGL244W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RUD3(YOR216C)|FD-Score:-5.49|P-value:2.00E-8||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC7(YDR389W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SCP160(YJL080C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEG1(YMR086W)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SNF12(YNR023W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SRO9(YCL037C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSP120(YLR250W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUR2(YDR297W)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUR4(YLR372W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCB3(YML072C)|FD-Score:-5|P-value:2.80E-7||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TES1(YJR019C)|FD-Score:-3.89|P-value:4.98E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TOS1(YBR162C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRP4(YDR354W)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YAP1801(YHR161C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR074W(YBR074W_p)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative metalloprotease Gene:YDR095C(YDR095C_d)|FD-Score:-4.42|P-value:4.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR020W(YFR020W_p)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL118C(YGL118C_d)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR269W(YGR269W_d)|FD-Score:-4.27|P-value:9.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YJL022W(YJL022W_d)|FD-Score:-5.68|P-value:6.78E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YLL044W(YLL044W_d)|FD-Score:-7.02|P-value:1.08E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR123C(YLR123C_d)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YLR402W(YLR402W_d)|FD-Score:-3.24|P-value:6.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL057W(YNL057W_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function Gene:YPL035C(YPL035C_d)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPR096C(YPR096C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:ADE4(YMR300C)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AQY2(YLL052C)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ATO3(YDR384C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:ATP18(YML081C-A)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms Gene:BAP2(YBR068C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BIM1(YER016W)|FD-Score:-8.33|P-value:3.99E-17||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BUD23(YCR047C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CDC73(YLR418C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-3.87|P-value:5.38E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CUR1(YPR158W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DBP7(YKR024C)|FD-Score:-4.58|P-value:2.37E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DEG1(YFL001W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DSF2(YBR007C_p)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG1(YFR044C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELP3(YPL086C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENO1(YGR254W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:EST2(YLR318W)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FLO8(YER109C)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FSF1(YOR271C_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:FYV12(YOR183W_p)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GIT1(YCR098C)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GTT2(YLL060C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HVG1(YER039C)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IOC4(YMR044W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC13(YOR235W_d)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:KEL1(YHR158C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LDB16(YCL005W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEM3(YNL323W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAL13(YGR288W)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MNS1(YJR131W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MRPL20(YKR085C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA1(YOR066W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSB2(YGR014W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSS51(YLR203C)|FD-Score:-3.81|P-value:6.96E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MTG1(YMR097C)|FD-Score:-17.3|P-value:1.95E-67||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NPC2(YDL046W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:PAC10(YGR078C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAT1(YCR077C)|FD-Score:-6.94|P-value:1.91E-12||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PCK1(YKR097W)|FD-Score:4.5|P-value:3.40E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PGM1(YKL127W)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:RCF2(YNR018W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RIM4(YHL024W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL26A(YLR344W)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTF1(YGL244W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RUD3(YOR216C)|FD-Score:-5.49|P-value:2.00E-8||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC7(YDR389W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SCP160(YJL080C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEG1(YMR086W)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SNF12(YNR023W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SRO9(YCL037C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSP120(YLR250W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUR2(YDR297W)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUR4(YLR372W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCB3(YML072C)|FD-Score:-5|P-value:2.80E-7||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TES1(YJR019C)|FD-Score:-3.89|P-value:4.98E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TOS1(YBR162C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRP4(YDR354W)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YAP1801(YHR161C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR074W(YBR074W_p)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative metalloprotease Gene:YDR095C(YDR095C_d)|FD-Score:-4.42|P-value:4.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR020W(YFR020W_p)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL118C(YGL118C_d)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR269W(YGR269W_d)|FD-Score:-4.27|P-value:9.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YJL022W(YJL022W_d)|FD-Score:-5.68|P-value:6.78E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YLL044W(YLL044W_d)|FD-Score:-7.02|P-value:1.08E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR123C(YLR123C_d)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YLR402W(YLR402W_d)|FD-Score:-3.24|P-value:6.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL057W(YNL057W_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function Gene:YPL035C(YPL035C_d)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPR096C(YPR096C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL143W6.562.64E-112.09CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YMR197C5.325.26E-82.09VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YLR249W3.236.16E-40.02YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YMR112C3.216.61E-40.11MED11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
YER168C3.109.69E-40.04CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YOR095C3.060.001110.00RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YJL085W3.060.001120.10EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YPL093W2.960.001550.03NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YMR131C2.920.001740.00RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YGL098W2.920.001750.00USE1Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
YLR086W2.920.001770.03SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YNR043W2.880.001960.01MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YJL025W2.870.002040.15RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YML093W2.720.003250.12UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDL193W2.600.004700.05NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W5.121.51E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YCR098C4.562.59E-6GIT1Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
YKR097W4.503.40E-6PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YMR300C4.415.27E-6ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YGL118C_d4.395.70E-6YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER167W4.318.18E-6BCK2Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
YNR023W4.102.06E-5SNF1273 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive
YLR372W4.032.76E-5SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YOR066W3.963.78E-5MSA1Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YGR288W3.914.54E-5MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YJL003W3.865.75E-5COX16Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
YER109C3.826.58E-5FLO8Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene
YBR007C_p3.787.93E-5DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YKR085C3.711.04E-4MRPL20Mitochondrial ribosomal protein of the large subunit
YNL057W_d3.621.46E-4YNL057W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1517
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2174.10E-64SGTC_14833453-0561 55.9 μMChemDiv (Drug-like library)45469910.0721649
0.0961.25E-13SGTC_7580868-0029 16.0 μMChemDiv (Drug-like library)7626870.0119048
0.0921.60E-12SGTC_28699037858 32.5 μMChembridge (Drug-like library)271870420.0434783
0.0881.25E-11SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.0731707RSC complex & mRNA processing
0.0848.52E-11SGTC_329k048-0121 21.3 μMChemDiv (Drug-like library)68329020.0116279RSC & ERG11
0.0831.53E-10SGTC_414piperonyl butoxide 100.0 μMMiscellaneous57940.0481928
0.0808.75E-10SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0519481
0.0791.03E-9SGTC_1472k841-0093 193.0 μMChemDiv (Drug-like library)X14720.0434783
0.0791.21E-9SGTC_15153460-0031 42.1 μMChemDiv (Drug-like library)28765960.106383DNA intercalators
0.0759.36E-9SGTC_13201313-0481 95.3 μMChemDiv (Drug-like library)68133650.0588235
0.0731.62E-8SGTC_421streptovitacin 30.0 mMMiscellaneous914670.109756
0.0654.96E-7SGTC_22216807166 181.1 μMChembridge (Fragment library)6749250.0470588
0.0631.16E-6SGTC_28529023854 52.0 μMChembridge (Drug-like library)197985280.0449438
0.0631.30E-6SGTC_28639028737 58.4 μMChembridge (Drug-like library)64609170.0652174
0.0621.62E-6SGTC_1560lithocholic acid 53.1 μMTimTec (Pure natural product library)99030.622951

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2531methyl deoxycholate70.07 μM116667744Microsource (Natural product library)406.598584.30724
SGTC_2555cholic acid100 μM0.7540986708657Microsource (Natural product library)422.597983.13835
SGTC_1560lithocholic acid53.1 μM0.6229519903TimTec (Pure natural product library)376.57265.18423
SGTC_2621lithochol-11-enic acid100 μM0.5142866708711Microsource (Natural product library)374.556724.73923
SGTC_2687chenodiol100 μM0.4925375702181Microsource (Natural product library)392.5724.01434
SGTC_1572epiandrosterone68.9 μM0.378788441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.378788441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.3787882754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2628lathosterol100 μM0.3333336710616Microsource (Natural product library)386.653547.37611
SGTC_1974st06944826.9 μM0.281696323587TimTec (Natural product derivative library)305.454943.36923
SGTC_1750st0818592.36 μM0.2537315291695TimTec (Natural product derivative library)275.472024.43511endomembrane recycling