esterone

3-hydroxy-13-methyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-17-one

A steroid.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1519
Screen concentration 74.0 μM
Source TimTec (Pure natural product library)
PubChem CID 698
SMILES CC12CCC3C(C1CCC2=O)CCC4=C3C=CC(=C4)O
Standardized SMILES CC12CCC3C(CCc4cc(O)ccc34)C1CCC2=O
Molecular weight 270.3661
ALogP 3.94
H-bond donor count 1
H-bond acceptor count 2
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.74
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 698
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.02||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BUR6(YER159C)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.09||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CEP3(YMR168C)|FD-Score:6.63|P-value:1.68E-11|Clearance:1.36||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:HRR25(YPL204W)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MCM3(YEL032W)|FD-Score:4.32|P-value:7.65E-6|Clearance:0.39||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:PDC2(YDR081C)|FD-Score:3.94|P-value:4.14E-5|Clearance:0.36||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RNR2(YJL026W)|FD-Score:-4.93|P-value:4.17E-7|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC19(YNL113W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPO26(YPR187W)|FD-Score:-4.97|P-value:3.42E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRN7(YJL025W)|FD-Score:5.27|P-value:6.71E-8|Clearance:0.72||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSC9(YML127W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.01||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:RVB2(YPL235W)|FD-Score:3.58|P-value:1.75E-4|Clearance:0.1||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC39(YLR440C)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.06||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEC53(YFL045C)|FD-Score:4.38|P-value:6.03E-6|Clearance:0.05||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:TOM22(YNL131W)|FD-Score:3.26|P-value:5.59E-4|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:VHT1(YGR065C)|FD-Score:3.33|P-value:4.37E-4|Clearance:0.07||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YDL196W(YDL196W_d)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR190C(YGR190C_d)|FD-Score:4.55|P-value:2.69E-6|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:ACC1(YNR016C)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.02||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BUR6(YER159C)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.09||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CEP3(YMR168C)|FD-Score:6.63|P-value:1.68E-11|Clearance:1.36||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:HRR25(YPL204W)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MCM3(YEL032W)|FD-Score:4.32|P-value:7.65E-6|Clearance:0.39||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:PDC2(YDR081C)|FD-Score:3.94|P-value:4.14E-5|Clearance:0.36||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RNR2(YJL026W)|FD-Score:-4.93|P-value:4.17E-7|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC19(YNL113W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPO26(YPR187W)|FD-Score:-4.97|P-value:3.42E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRN7(YJL025W)|FD-Score:5.27|P-value:6.71E-8|Clearance:0.72||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSC9(YML127W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.01||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:RVB2(YPL235W)|FD-Score:3.58|P-value:1.75E-4|Clearance:0.1||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC39(YLR440C)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.06||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEC53(YFL045C)|FD-Score:4.38|P-value:6.03E-6|Clearance:0.05||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:TOM22(YNL131W)|FD-Score:3.26|P-value:5.59E-4|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:VHT1(YGR065C)|FD-Score:3.33|P-value:4.37E-4|Clearance:0.07||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YDL196W(YDL196W_d)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR190C(YGR190C_d)|FD-Score:4.55|P-value:2.69E-6|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 698
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ABZ2(YMR289W)|FD-Score:6.17|P-value:3.46E-10||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AHC2(YCR082W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALE1(YOR175C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:AQY2(YLL052C)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARO2(YGL148W)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ART10(YLR392C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:CHL4(YDR254W)|FD-Score:-5.23|P-value:8.43E-8||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:CKI1(YLR133W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CTK1(YKL139W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CWP2(YKL096W-A)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DGR1(YNL130C-A_p)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DSF2(YBR007C_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM31(YBR176W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EFM1(YHL039W)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:FPS1(YLL043W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:GEA1(YJR031C)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:HAP2(YGL237C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HBT1(YDL223C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HFA1(YMR207C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HST1(YOL068C)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTL1(YCR020W-B)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:ISY1(YJR050W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KIN4(YOR233W)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:MAG2(YLR427W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MFT1(YML062C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MKT1(YNL085W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MOS2(YGR235C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRP13(YGR084C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL32(YCR003W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSC2(YDR205W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:NSG1(YHR133C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PAU7(YAR020C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PER1(YCR044C)|FD-Score:6.35|P-value:1.05E-10||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX15(YOL044W)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PKH1(YDR490C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:POP2(YNR052C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PST2(YDR032C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAD34(YDR314C)|FD-Score:4.29|P-value:8.96E-6||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:REH1(YLR387C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RGC1(YPR115W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RMR1(YGL250W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus Gene:RPL1B(YGL135W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL24A(YGL031C)|FD-Score:7.5|P-value:3.08E-14||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSC1(YGR056W)|FD-Score:4.92|P-value:4.34E-7||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAF1(YBR280C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SCO2(YBR024W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SIZ1(YDR409W)|FD-Score:4.42|P-value:4.84E-6||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SPC1(YJR010C-A)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SSD1(YDR293C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TDH2(YJR009C)|FD-Score:-4.39|P-value:5.59E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TGS1(YPL157W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TMA64(YDR117C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPK2(YPL203W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:UBX2(YML013W)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VID30(YGL227W)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VNX1(YNL321W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL002C(YCL002C_p)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR054C(YFR054C_d)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL041C(YGL041C_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL118C(YGL118C_d)|FD-Score:6.71|P-value:9.62E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL185C(YGL185C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGL218W(YGL218W_d)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR107W(YGR107W_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YGR291C(YGR291C_d)|FD-Score:-4.94|P-value:3.88E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL182C(YJL182C_d)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YLR123C(YLR123C_d)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YMR166C(YMR166C_p)|FD-Score:5.77|P-value:3.86E-9||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNR029C(YNR029C_p)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR225W(YOR225W_d)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL108W(YPL108W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:AAT2(YLR027C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ABZ2(YMR289W)|FD-Score:6.17|P-value:3.46E-10||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AHC2(YCR082W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALE1(YOR175C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:AQY2(YLL052C)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARO2(YGL148W)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ART10(YLR392C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:CHL4(YDR254W)|FD-Score:-5.23|P-value:8.43E-8||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:CKI1(YLR133W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CTK1(YKL139W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CWP2(YKL096W-A)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DGR1(YNL130C-A_p)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DSF2(YBR007C_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM31(YBR176W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EFM1(YHL039W)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:FPS1(YLL043W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:GEA1(YJR031C)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:HAP2(YGL237C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HBT1(YDL223C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HFA1(YMR207C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HST1(YOL068C)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTL1(YCR020W-B)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:ISY1(YJR050W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KIN4(YOR233W)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:MAG2(YLR427W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MFT1(YML062C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MKT1(YNL085W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MOS2(YGR235C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRP13(YGR084C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL32(YCR003W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSC2(YDR205W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:NSG1(YHR133C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PAU7(YAR020C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PER1(YCR044C)|FD-Score:6.35|P-value:1.05E-10||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX15(YOL044W)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PKH1(YDR490C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:POP2(YNR052C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PST2(YDR032C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAD34(YDR314C)|FD-Score:4.29|P-value:8.96E-6||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:REH1(YLR387C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RGC1(YPR115W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RMR1(YGL250W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus Gene:RPL1B(YGL135W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL24A(YGL031C)|FD-Score:7.5|P-value:3.08E-14||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSC1(YGR056W)|FD-Score:4.92|P-value:4.34E-7||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAF1(YBR280C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SCO2(YBR024W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SIZ1(YDR409W)|FD-Score:4.42|P-value:4.84E-6||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SPC1(YJR010C-A)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SSD1(YDR293C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TDH2(YJR009C)|FD-Score:-4.39|P-value:5.59E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TGS1(YPL157W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TMA64(YDR117C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPK2(YPL203W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:UBX2(YML013W)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VID30(YGL227W)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VNX1(YNL321W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL002C(YCL002C_p)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR054C(YFR054C_d)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL041C(YGL041C_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL118C(YGL118C_d)|FD-Score:6.71|P-value:9.62E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL185C(YGL185C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGL218W(YGL218W_d)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR107W(YGR107W_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YGR291C(YGR291C_d)|FD-Score:-4.94|P-value:3.88E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL182C(YJL182C_d)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YLR123C(YLR123C_d)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YMR166C(YMR166C_p)|FD-Score:5.77|P-value:3.86E-9||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNR029C(YNR029C_p)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR225W(YOR225W_d)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL108W(YPL108W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR168C6.631.68E-111.36CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YJL025W5.276.71E-80.72RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YGR190C_d4.552.69E-60.17YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YFL045C4.386.03E-60.05SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YEL032W4.327.65E-60.39MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YDR081C3.944.14E-50.36PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YPL235W3.581.75E-40.10RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YLR440C3.482.55E-40.06SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YER159C3.423.16E-40.09BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YGR065C3.334.37E-40.07VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YNL131W3.265.59E-40.06TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YNL113W3.206.81E-40.07RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YNR016C3.138.63E-40.02ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YDL196W_d3.119.28E-40.02YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YER125W3.099.98E-40.01RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL031C7.503.08E-14RPL24ARibosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
YGL118C_d6.719.62E-12YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR044C6.351.05E-10PER1Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
YMR289W6.173.46E-10ABZ2Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
YOR133W5.981.10E-9EFT1Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
YMR166C_p5.773.86E-9YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YHL039W5.334.87E-8EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YGL148W5.042.34E-7ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YGR056W4.924.34E-7RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YOL044W4.464.19E-6PEX15Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly
YDR409W4.424.84E-6SIZ1SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
YDL223C4.415.20E-6HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YDR314C4.298.96E-6RAD34Protein involved in nucleotide excision repair (NER); homologous to RAD4
YGL218W_d4.211.27E-5YGL218W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
YLR387C4.201.31E-5REH1Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains

GO enrichment analysis for SGTC_1519
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3581.23E-177SGTC_264promethazine 12.5 mMMiscellaneous60140.0606061DNA intercalators
0.3122.93E-133SGTC_15153460-0031 42.1 μMChemDiv (Drug-like library)28765960.0823529DNA intercalators
0.2888.80E-113SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.112676DNA intercalators
0.2621.80E-92SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.0909091DNA intercalators
0.2451.29E-81SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.0945946DNA intercalators
0.2202.43E-65SGTC_14873970-0795 2.8 μMChemDiv (Drug-like library)37651030.106061DNA intercalators
0.2109.29E-60SGTC_1468k284-1086 134.0 μMChemDiv (Drug-like library)159964570.0493827DNA intercalators
0.1771.57E-42SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.107692DNA intercalators
0.1632.07E-36SGTC_257daunorubicin 18.5 μMMiscellaneous303230.111111anthracycline transcription coupled DNA repair
0.1575.51E-34SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0232558DNA intercalators
0.1553.19E-33SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.0581395
0.1532.46E-32SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.121212DNA intercalators
0.1331.42E-24SGTC_33109130229 44.1 μMChembridge (Drug-like library)77612580.0810811Golgi
0.1224.36E-21SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.0597015DNA intercalators
0.1134.38E-18SGTC_1434032-1194 15.7 μMChemDiv (Drug-like library)18069630.126761

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_406estrone100 μM15870Miscellaneous270.366083.93812
SGTC_1767st04499270.3 μM0.79600693TimTec (Natural product derivative library)284.395963.50123
SGTC_231b-estradiol524 μM0.62450Miscellaneous272.381963.83822
SGTC_1587estradiol valerate56.1 μM0.513791TimTec (Pure natural product library)356.498385.79613
SGTC_416estradiol valerate100 μM0.513791TimTec (Pure natural product library)356.498385.79613
SGTC_2678equilin100 μM0.53247Microsource (Natural product library)268.35023.68812
SGTC_2650prasterone100 μM0.41071416759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.4107145881Miscellaneous288.424423.33812
SGTC_417st075190100 μM0.402985222757TimTec (Pure natural product library)376.488025.51213
SGTC_1572epiandrosterone68.9 μM0.385965441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.385965441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.3859652754130Microsource (Natural product library)290.44033.58812cell wall