flindersine

2,2-dimethyl-6H-pyrano[3,2-c]quinolin-5-one

Flindersine is a natural alkaloid of the quinoline subtype.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1520
Screen concentration 88.0 μM
Source TimTec (Pure natural product library)
PubChem CID 68230
SMILES CC1(C=CC2=C(O1)C3=CC=CC=C3NC2=O)C
Standardized SMILES CC1(C)Oc2c(C=C1)c(O)nc3ccccc23
Molecular weight 227.2585
ALogP 1.7
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.95
% growth inhibition (Hom. pool) 6.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 68230
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.18||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:4.77|P-value:9.14E-7|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:5.24|P-value:7.91E-8|Clearance:0.47||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC25(YLR310C)|FD-Score:4.33|P-value:7.58E-6|Clearance:0.17||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:ERB1(YMR049C)|FD-Score:3.78|P-value:7.77E-5|Clearance:0.06||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG12(YMR208W)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.15||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG13(YML126C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.01||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:FCP1(YMR277W)|FD-Score:3.72|P-value:9.84E-5|Clearance:0.19||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GCR1(YPL075W)|FD-Score:-4.25|P-value:1.08E-5|Clearance:0||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:MAK5(YBR142W)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MED11(YMR112C)|FD-Score:3.9|P-value:4.81E-5|Clearance:0.12||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:NDC1(YML031W)|FD-Score:4.64|P-value:1.77E-6|Clearance:0.15||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP9(YJL010C)|FD-Score:-4.64|P-value:1.75E-6|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NUP85(YJR042W)|FD-Score:-3.76|P-value:8.58E-5|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PGA1(YNL158W)|FD-Score:-3.87|P-value:5.50E-5|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PKC1(YBL105C)|FD-Score:-3.21|P-value:6.67E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPP1(YHR062C)|FD-Score:7.59|P-value:1.55E-14|Clearance:2.35||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:4.15|P-value:1.65E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS5(YJR123W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC58(YLR033W)|FD-Score:-4.33|P-value:7.48E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC21(YNL287W)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.13||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SLD5(YDR489W)|FD-Score:-4.78|P-value:8.80E-7|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SNM1(YDR478W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPN1(YPR133C)|FD-Score:4.49|P-value:3.64E-6|Clearance:0.16||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRB4(YER022W)|FD-Score:-4.1|P-value:2.03E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TFB3(YDR460W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TIF11(YMR260C)|FD-Score:4.76|P-value:9.55E-7|Clearance:0.13||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:UTP4(YDR324C)|FD-Score:10.4|P-value:1.17E-25|Clearance:2.81||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YCG1(YDR325W)|FD-Score:-3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YPR136C(YPR136C_d)|FD-Score:4.07|P-value:2.38E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:YTH1(YPR107C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:CCT2(YIL142W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.18||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:4.77|P-value:9.14E-7|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:5.24|P-value:7.91E-8|Clearance:0.47||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC25(YLR310C)|FD-Score:4.33|P-value:7.58E-6|Clearance:0.17||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:ERB1(YMR049C)|FD-Score:3.78|P-value:7.77E-5|Clearance:0.06||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG12(YMR208W)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.15||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG13(YML126C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.01||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:FCP1(YMR277W)|FD-Score:3.72|P-value:9.84E-5|Clearance:0.19||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GCR1(YPL075W)|FD-Score:-4.25|P-value:1.08E-5|Clearance:0||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:MAK5(YBR142W)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MED11(YMR112C)|FD-Score:3.9|P-value:4.81E-5|Clearance:0.12||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:NDC1(YML031W)|FD-Score:4.64|P-value:1.77E-6|Clearance:0.15||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP9(YJL010C)|FD-Score:-4.64|P-value:1.75E-6|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NUP85(YJR042W)|FD-Score:-3.76|P-value:8.58E-5|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PGA1(YNL158W)|FD-Score:-3.87|P-value:5.50E-5|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PKC1(YBL105C)|FD-Score:-3.21|P-value:6.67E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPP1(YHR062C)|FD-Score:7.59|P-value:1.55E-14|Clearance:2.35||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:4.15|P-value:1.65E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS5(YJR123W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC58(YLR033W)|FD-Score:-4.33|P-value:7.48E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC21(YNL287W)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.13||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SLD5(YDR489W)|FD-Score:-4.78|P-value:8.80E-7|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SNM1(YDR478W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPN1(YPR133C)|FD-Score:4.49|P-value:3.64E-6|Clearance:0.16||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRB4(YER022W)|FD-Score:-4.1|P-value:2.03E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TFB3(YDR460W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TIF11(YMR260C)|FD-Score:4.76|P-value:9.55E-7|Clearance:0.13||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:UTP4(YDR324C)|FD-Score:10.4|P-value:1.17E-25|Clearance:2.81||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YCG1(YDR325W)|FD-Score:-3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YPR136C(YPR136C_d)|FD-Score:4.07|P-value:2.38E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:YTH1(YPR107C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 68230
Download HOP data (tab-delimited text)  (excel)
Gene:ADD66(YKL206C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ADY3(YDL239C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:ALO1(YML086C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ANS1(YHR126C_p)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARO1(YDR127W)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:7.88|P-value:1.69E-15||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ART10(YLR392C)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:AXL1(YPR122W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BOP2(YLR267W_p)|FD-Score:4.47|P-value:3.93E-6||SGD DESC:Protein of unknown function Gene:BSC6(YOL137W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CCZ1(YBR131W)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CIS3(YJL158C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:COA6(YMR244C-A_p)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CTK1(YKL139W)|FD-Score:5.73|P-value:5.04E-9||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL81(YIR023W)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM27(YJR106W)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:ERG2(YMR202W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FAR1(YJL157C)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FMP21(YBR269C_p)|FD-Score:-3.8|P-value:7.20E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:5.89|P-value:1.94E-9||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.19|P-value:1.27E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GRR1(YJR090C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HEH2(YDR458C)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IRS4(YKR019C)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LEO1(YOR123C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LIA1(YJR070C)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LST4(YKL176C)|FD-Score:-4.87|P-value:5.56E-7||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAG2(YLR427W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MDM20(YOL076W)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MND2(YIR025W)|FD-Score:4.31|P-value:8.27E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:NCS2(YNL119W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NTE1(YML059C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OMS1(YDR316W)|FD-Score:6.5|P-value:4.03E-11||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PAU5(YFL020C)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PDC5(YLR134W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PIB2(YGL023C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PPH3(YDR075W)|FD-Score:-4.44|P-value:4.44E-6||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PRS2(YER099C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAD26(YJR035W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RIT1(YMR283C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL14B(YHL001W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RUB1(YDR139C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SAC6(YDR129C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDH4(YDR178W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGO1(YOR073W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHE4(YOR035C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SKI3(YPR189W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SNF12(YNR023W)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNT1(YCR033W)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SOH1(YGL127C)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SWA2(YDR320C)|FD-Score:-5.36|P-value:4.27E-8||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TFB6(YOR352W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TOM7(YNL070W)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM2(YKR056W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP1(YDR007W)|FD-Score:9.5|P-value:1.03E-21||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:11.6|P-value:2.69E-31||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:11.5|P-value:6.75E-31||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:10.1|P-value:3.43E-24||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:URN1(YPR152C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VAC14(YLR386W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS20(YMR077C)|FD-Score:-4.3|P-value:8.71E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YCL049C(YCL049C_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEN1(YER041W)|FD-Score:-5.07|P-value:1.97E-7||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFT2(YDR319C_p)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL117W(YGL117W_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function Gene:YGR069W(YGR069W_d)|FD-Score:6|P-value:9.95E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL016W(YJL016W_p)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR061W(YJR061W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YJR111C(YJR111C_p)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKL202W(YKL202W_d)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR307C-A(YLR307C-A_p)|FD-Score:-6.89|P-value:2.81E-12||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR103C(YMR103C_d)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL120C(YNL120C_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YPL071C(YPL071C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPR096C(YPR096C)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPR147C(YPR147C_p)|FD-Score:-6.15|P-value:3.89E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YPR170C(YPR170C_d)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YSW1(YBR148W)|FD-Score:-4.46|P-value:4.14E-6||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:ADD66(YKL206C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ADY3(YDL239C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:ALO1(YML086C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ANS1(YHR126C_p)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARO1(YDR127W)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:7.88|P-value:1.69E-15||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ART10(YLR392C)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:AXL1(YPR122W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BOP2(YLR267W_p)|FD-Score:4.47|P-value:3.93E-6||SGD DESC:Protein of unknown function Gene:BSC6(YOL137W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CCZ1(YBR131W)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CIS3(YJL158C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:COA6(YMR244C-A_p)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CTK1(YKL139W)|FD-Score:5.73|P-value:5.04E-9||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL81(YIR023W)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM27(YJR106W)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:ERG2(YMR202W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FAR1(YJL157C)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FMP21(YBR269C_p)|FD-Score:-3.8|P-value:7.20E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:5.89|P-value:1.94E-9||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.19|P-value:1.27E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GRR1(YJR090C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HEH2(YDR458C)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IRS4(YKR019C)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LEO1(YOR123C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LIA1(YJR070C)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LST4(YKL176C)|FD-Score:-4.87|P-value:5.56E-7||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAG2(YLR427W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MDM20(YOL076W)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MND2(YIR025W)|FD-Score:4.31|P-value:8.27E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:NCS2(YNL119W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NTE1(YML059C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OMS1(YDR316W)|FD-Score:6.5|P-value:4.03E-11||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PAU5(YFL020C)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PDC5(YLR134W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PIB2(YGL023C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PPH3(YDR075W)|FD-Score:-4.44|P-value:4.44E-6||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PRS2(YER099C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAD26(YJR035W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RIT1(YMR283C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL14B(YHL001W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RUB1(YDR139C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SAC6(YDR129C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDH4(YDR178W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGO1(YOR073W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHE4(YOR035C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SKI3(YPR189W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SNF12(YNR023W)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNT1(YCR033W)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SOH1(YGL127C)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SWA2(YDR320C)|FD-Score:-5.36|P-value:4.27E-8||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TFB6(YOR352W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TOM7(YNL070W)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM2(YKR056W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP1(YDR007W)|FD-Score:9.5|P-value:1.03E-21||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:11.6|P-value:2.69E-31||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:11.5|P-value:6.75E-31||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:10.1|P-value:3.43E-24||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:URN1(YPR152C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VAC14(YLR386W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS20(YMR077C)|FD-Score:-4.3|P-value:8.71E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YCL049C(YCL049C_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEN1(YER041W)|FD-Score:-5.07|P-value:1.97E-7||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFT2(YDR319C_p)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL117W(YGL117W_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function Gene:YGR069W(YGR069W_d)|FD-Score:6|P-value:9.95E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL016W(YJL016W_p)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR061W(YJR061W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YJR111C(YJR111C_p)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKL202W(YKL202W_d)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR307C-A(YLR307C-A_p)|FD-Score:-6.89|P-value:2.81E-12||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR103C(YMR103C_d)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL120C(YNL120C_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YPL071C(YPL071C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPR096C(YPR096C)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPR147C(YPR147C_p)|FD-Score:-6.15|P-value:3.89E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YPR170C(YPR170C_d)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YSW1(YBR148W)|FD-Score:-4.46|P-value:4.14E-6||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR324C10.401.17E-252.81UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YHR062C7.591.55E-142.35RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJL111W5.247.91E-80.47CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YDR188W4.779.14E-70.01CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YMR260C4.769.55E-70.13TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YML031W4.641.77E-60.15NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YPR133C4.493.64E-60.16SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YLR310C4.337.58E-60.17CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YOL040C4.151.65E-50.08RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YPR136C_d4.072.38E-50.17YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YMR112C3.904.81E-50.12MED11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
YMR049C3.787.77E-50.06ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YMR277W3.729.84E-50.18FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YMR208W3.542.02E-40.15ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YML126C3.393.53E-40.01ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W11.602.69E-31TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKL211C11.506.75E-31TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W10.103.43E-24TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR007W9.501.03E-21TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YEL009C8.191.27E-16GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR035W7.881.69E-15ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YDR316W6.504.03E-11OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YGR069W_d6.009.95E-10YGR069W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR283C5.891.94E-9GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YKL139W5.735.04E-9CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YOL076W5.171.14E-7MDM20Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
YDR008C_d5.131.43E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJL157C5.062.14E-7FAR1Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress
YKR019C4.983.11E-7IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YIR023W4.836.70E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism

GO enrichment analysis for SGTC_1520
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4172.67E-247SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.107692
0.3961.45E-220SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0789474
0.3874.60E-210SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.078125
0.3791.22E-200SGTC_18825615643 20.0 μMMiscellaneous22530750.0769231TRP & mitochondrial translation
0.3751.01E-196SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.147541
0.3743.98E-195SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.037037
0.3737.88E-194SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0481928
0.3727.28E-193SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0909091
0.3706.87E-191SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0983607
0.3681.14E-188SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.15
0.3665.07E-187SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.107692
0.3622.17E-182SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.09375
0.3611.54E-181SGTC_18815652484 16.0 μMMiscellaneous22551290.0921053TRP & mitochondrial translation
0.3584.96E-178SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.09375
0.3577.65E-177SGTC_24885268135 45.7 μMMiscellaneous12703560.178571

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22226885208172.14 μM0.3609514Chembridge (Fragment library)233.26311.43313
SGTC_20405100729123 μM0.28888911840Chembridge (Fragment library)161.157381.15612
SGTC_289k017-0060526 μM0.2857145512909ChemDiv (Drug-like library)380.21651.56725
SGTC_2528xanthyletin44.79 μM0.2865188Microsource (Natural product library)228.243282.68703
SGTC_900870-08634.61 μM0.2758621738164ChemDiv (Drug-like library)311.762263.53812
SGTC_23799035213200 μM0.272727646551Chembridge (Fragment library)240.300342.32213
SGTC_20375169046200 μM0.2641515718022Chembridge (Fragment library)204.22518-0.0923460S ribosome export
SGTC_20545233173129 μM0.260876889Chembridge (Fragment library)226.026861.41812mitochondrial response to ROS
SGTC_235890371574 μM0.2592592388925Chembridge (Fragment library)231.250520.20513
SGTC_2706st07823982.5 μM0.25925915434492TimTec (Natural product derivative library)242.273161.08712