st012844

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1523
Screen concentration 44.7 μM
Source TimTec (Pure natural product library)
PubChem CID 4274103
SMILES CC1=C(C(=O)N(N1C)C2=CC=CC=C2)N=CC(CN3CC4CC(C3)C5=CC=CC(=O)N5C4)O
Standardized SMILES CN1N(C(=O)C(=C1C)N=CC(O)CN2CC3CC(C2)C4=CC=CC(=O)N4C3)c5ccccc5
Molecular weight 447.5295
ALogP 0.23
H-bond donor count 1
H-bond acceptor count 6
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.26
% growth inhibition (Hom. pool) 2.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4274103
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:-7.69|P-value:7.08E-15|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CCT5(YJR064W)|FD-Score:3.73|P-value:9.70E-5|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CYR1(YJL005W)|FD-Score:-6.92|P-value:2.29E-12|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:GCD6(YDR211W)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.1||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:INO80(YGL150C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:KAR1(YNL188W)|FD-Score:-5.16|P-value:1.22E-7|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:LSG1(YGL099W)|FD-Score:4.31|P-value:8.19E-6|Clearance:0.2||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MES1(YGR264C)|FD-Score:-4.06|P-value:2.47E-5|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MEX67(YPL169C)|FD-Score:-3.21|P-value:6.72E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NMD3(YHR170W)|FD-Score:6.39|P-value:8.29E-11|Clearance:2.08||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE5(YML023C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.16||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PIK1(YNL267W)|FD-Score:-4.36|P-value:6.56E-6|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RIX1(YHR197W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.14||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPP1(YHR062C)|FD-Score:3.95|P-value:3.87E-5|Clearance:0.23||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SAD1(YFR005C)|FD-Score:3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SAH1(YER043C)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor Gene:SDA1(YGR245C)|FD-Score:-3.73|P-value:9.54E-5|Clearance:0||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SLD5(YDR489W)|FD-Score:-3.94|P-value:4.07E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SRP21(YKL122C)|FD-Score:-5.2|P-value:1.01E-7|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:SRP72(YPL210C)|FD-Score:-7.19|P-value:3.26E-13|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TIF5(YPR041W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.08||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL111C(YKL111C_d)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.22||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:ARP2(YDL029W)|FD-Score:-7.69|P-value:7.08E-15|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CCT5(YJR064W)|FD-Score:3.73|P-value:9.70E-5|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CYR1(YJL005W)|FD-Score:-6.92|P-value:2.29E-12|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:GCD6(YDR211W)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.1||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:INO80(YGL150C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:KAR1(YNL188W)|FD-Score:-5.16|P-value:1.22E-7|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:LSG1(YGL099W)|FD-Score:4.31|P-value:8.19E-6|Clearance:0.2||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MES1(YGR264C)|FD-Score:-4.06|P-value:2.47E-5|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MEX67(YPL169C)|FD-Score:-3.21|P-value:6.72E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NMD3(YHR170W)|FD-Score:6.39|P-value:8.29E-11|Clearance:2.08||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE5(YML023C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.16||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PIK1(YNL267W)|FD-Score:-4.36|P-value:6.56E-6|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RIX1(YHR197W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.14||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPP1(YHR062C)|FD-Score:3.95|P-value:3.87E-5|Clearance:0.23||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SAD1(YFR005C)|FD-Score:3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SAH1(YER043C)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor Gene:SDA1(YGR245C)|FD-Score:-3.73|P-value:9.54E-5|Clearance:0||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SLD5(YDR489W)|FD-Score:-3.94|P-value:4.07E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SRP21(YKL122C)|FD-Score:-5.2|P-value:1.01E-7|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:SRP72(YPL210C)|FD-Score:-7.19|P-value:3.26E-13|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TIF5(YPR041W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.08||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL111C(YKL111C_d)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.22||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4274103
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ALG12(YNR030W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APD1(YBR151W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:APP1(YNL094W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATP11(YNL315C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BNA3(YJL060W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BUD27(YFL023W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CDC55(YGL190C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CRS5(YOR031W)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DDR2(YOL052C-A)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:END3(YNL084C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERV29(YGR284C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FMP21(YBR269C_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE5(YOR384W)|FD-Score:-3.73|P-value:9.71E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUS2(YMR232W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GLR1(YPL091W)|FD-Score:4.35|P-value:6.71E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HNT2(YDR305C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HOP1(YIL072W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:ICS3(YJL077C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:KGD1(YIL125W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:KTR2(YKR061W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:MET16(YPR167C)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MRP13(YGR084C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC7(YEL033W_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NKP1(YDR383C)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NMD2(YHR077C)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NRP1(YDL167C)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUP133(YKR082W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NUP157(YER105C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication Gene:OGG1(YML060W)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PAL1(YDR348C)|FD-Score:6.6|P-value:2.08E-11||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PHO23(YNL097C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PLB3(YOL011W)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:POM33(YLL023C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PSK1(YAL017W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCY1(YJL204C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO2(YNL090W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIM20(YOR275C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RPS7A(YOR096W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SPH1(YLR313C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPI1(YER150W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO75(YLL005C)|FD-Score:5.68|P-value:6.85E-9||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPT10(YJL127C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SSK2(YNR031C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:STO1(YMR125W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWH1(YAR042W)|FD-Score:6.45|P-value:5.58E-11||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:TDA1(YMR291W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:THI7(YLR237W)|FD-Score:-3.73|P-value:9.65E-5||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TOK1(YJL093C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:UBP3(YER151C)|FD-Score:4.37|P-value:6.33E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBP9(YER098W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VFA1(YER128W)|FD-Score:-4.18|P-value:1.43E-5||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VIK1(YPL253C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS61(YDR136C_d)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WHI3(YNL197C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:XDJ1(YLR090W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR184W(YBR184W_p)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL129W(YDL129W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL144C(YDL144C_p)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YGR035W-A(YGR035W-A_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function Gene:YGR174W-A(YGR174W-A_p)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YGR266W(YGR266W)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL182C(YJL182C_d)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YLR118C(YLR118C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YML122C(YML122C_d)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR130W(YMR130W_p)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:3.72|P-value:9.85E-5||SGD DESC:Putative protein of unknown function Gene:YMR316C-B(YMR316C-B_d)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-4.55|P-value:2.64E-6||SGD DESC:Putative protein of unknown function Gene:YOR019W(YOR019W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR283W(YOR283W)|FD-Score:-3.96|P-value:3.75E-5||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YSW1(YBR148W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YTP1(YNL237W)|FD-Score:5|P-value:2.79E-7||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZAP1(YJL056C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AAP1(YHR047C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ALG12(YNR030W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APD1(YBR151W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:APP1(YNL094W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATP11(YNL315C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BNA3(YJL060W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BUD27(YFL023W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CDC55(YGL190C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CRS5(YOR031W)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DDR2(YOL052C-A)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:END3(YNL084C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERV29(YGR284C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FMP21(YBR269C_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE5(YOR384W)|FD-Score:-3.73|P-value:9.71E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUS2(YMR232W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GLR1(YPL091W)|FD-Score:4.35|P-value:6.71E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HNT2(YDR305C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HOP1(YIL072W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:ICS3(YJL077C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:KGD1(YIL125W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:KTR2(YKR061W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:MET16(YPR167C)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MRP13(YGR084C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC7(YEL033W_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NKP1(YDR383C)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NMD2(YHR077C)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NRP1(YDL167C)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUP133(YKR082W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NUP157(YER105C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication Gene:OGG1(YML060W)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PAL1(YDR348C)|FD-Score:6.6|P-value:2.08E-11||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PHO23(YNL097C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PLB3(YOL011W)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:POM33(YLL023C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PSK1(YAL017W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCY1(YJL204C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO2(YNL090W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIM20(YOR275C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RPS7A(YOR096W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SPH1(YLR313C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPI1(YER150W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO75(YLL005C)|FD-Score:5.68|P-value:6.85E-9||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPT10(YJL127C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SSK2(YNR031C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:STO1(YMR125W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWH1(YAR042W)|FD-Score:6.45|P-value:5.58E-11||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:TDA1(YMR291W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:THI7(YLR237W)|FD-Score:-3.73|P-value:9.65E-5||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TOK1(YJL093C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:UBP3(YER151C)|FD-Score:4.37|P-value:6.33E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBP9(YER098W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VFA1(YER128W)|FD-Score:-4.18|P-value:1.43E-5||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VIK1(YPL253C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS61(YDR136C_d)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WHI3(YNL197C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:XDJ1(YLR090W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR184W(YBR184W_p)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL129W(YDL129W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL144C(YDL144C_p)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YGR035W-A(YGR035W-A_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function Gene:YGR174W-A(YGR174W-A_p)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YGR266W(YGR266W)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL182C(YJL182C_d)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YLR118C(YLR118C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YML122C(YML122C_d)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR130W(YMR130W_p)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:3.72|P-value:9.85E-5||SGD DESC:Putative protein of unknown function Gene:YMR316C-B(YMR316C-B_d)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-4.55|P-value:2.64E-6||SGD DESC:Putative protein of unknown function Gene:YOR019W(YOR019W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR283W(YOR283W)|FD-Score:-3.96|P-value:3.75E-5||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YSW1(YBR148W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YTP1(YNL237W)|FD-Score:5|P-value:2.79E-7||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZAP1(YJL056C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W6.398.29E-112.08NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W4.318.19E-60.20LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YML023C4.111.94E-50.16NSE5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YHR062C3.953.87E-50.23RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJR064W3.739.70E-50.11CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YHR197W3.621.50E-40.14RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YKL111C_d3.482.52E-40.22YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YFR005C3.265.66E-40.00SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YPR041W3.255.70E-40.08TIF5Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
YDR211W3.177.61E-40.10GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YJR042W3.070.001080.06NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YNL088W3.010.001310.09TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YGR002C2.920.001750.04SWC4Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
YIL026C2.880.002000.03IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YMR220W2.850.002220.17ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR348C6.602.08E-11PAL1Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
YAR042W6.455.58E-11SWH1Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
YLL005C5.686.85E-9SPO75Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
YJL077C5.191.07E-7ICS3Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2
YNL237W5.002.79E-7YTP1Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
YDL144C_p4.612.06E-6YDL144C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
YNL090W4.572.43E-6RHO2Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
YOR031W4.473.86E-6CRS5Copper-binding metallothionein, required for wild-type copper resistance
YOL052C-A4.424.85E-6DDR2Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
YER151C4.376.33E-6UBP3Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
YPL091W4.356.71E-6GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YGR035W-A_p4.261.01E-5YGR035W-A_pPutative protein of unknown function
YNL197C4.251.09E-5WHI3RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication
YDL129W_p4.191.40E-5YDL129W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
YOR275C4.082.22E-5RIM20Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation

GO enrichment analysis for SGTC_1523
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0984.55E-14SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.081632760S ribosome export
0.0921.41E-12SGTC_28097964133 13.0 μMChembridge (Drug-like library)12536650.085714360S ribosome export
0.0904.61E-12SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10576960S ribosome export
0.0841.07E-10SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.084210560S ribosome export
0.0814.97E-10SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.069565260S ribosome export
0.0806.10E-10SGTC_22047233102 200.0 μMChembridge (Fragment library)21724800.096774260S ribosome export
0.0807.98E-10SGTC_13833049-0032 20.7 μMChemDiv (Drug-like library)7128640.086021560S ribosome export
0.0791.30E-9SGTC_13611608-0041 5.6 μMChemDiv (Drug-like library)X13610.0531915RPP1 & pyrimidine depletion
0.0781.61E-9SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.074468160S ribosome export
0.0758.52E-9SGTC_1081sertraline 6.0 μMNIH Clinical Collection630090.087912160S ribosome export
0.0741.08E-8SGTC_32609137109 49.5 μMChembridge (Drug-like library)174783460.11363660S ribosome export
0.0732.17E-8SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.091836760S ribosome export
0.0722.97E-8SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.071428660S ribosome export
0.0722.97E-8SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.071428660S ribosome export
0.0723.75E-8SGTC_5700987-0065 62.6 μMChemDiv (Drug-like library)31044280.085106460S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1522st01284245.6 μM0.610393491067TimTec (Pure natural product library)438.520942.65815RNA pol III & RNase P/MRP
SGTC_1521st01294249.4 μM0.45070424207259TimTec (Pure natural product library)404.776780.21635
SGTC_1673672-005166.21 μM0.1979173237971ChemDiv (Drug-like library)366.49654.56514
SGTC_3341502-102468.5 μM0.1847833296216ChemDiv (Drug-like library)352.469924.07814
SGTC_10583448-542597.3 μM0.1829277279705ChemDiv (Drug-like library)262.305863.71602
SGTC_12360330-0013258 μM0.1785714617066ChemDiv (Drug-like library)262.347381.96212
SGTC_2474532157043.32 μM0.1764712841384Miscellaneous297.37153.55303
SGTC_5891227-007034.1 μM0.1764712841384ChemDiv (Drug-like library)297.37153.55303
SGTC_511blebbistatin171 μM0.1739133476986ICCB bioactive library292.331842.42613DNA intercalators
SGTC_21725754775163.92 μM0.1686752263357Chembridge (Fragment library)257.327561.72113