arabinosylhypoxanthine

9-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-3H-purin-6-one

An inhibitor of viral and cellular DNA polymerases.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1528
Screen concentration 74.6 μM
Source TimTec (Pure natural product library)
PubChem CID 804
SMILES C1=NC(=O)C2=C(N1)N(C=N2)C3C(C(C(O3)CO)O)O
Standardized SMILES OCC1OC(C(O)C1O)n2cnc3c(O)ncnc23
Molecular weight 268.2261
ALogP -2.25
H-bond donor count 4
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.28
% growth inhibition (Hom. pool) -4.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 804
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB3(YKL088W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.03||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CCA1(YER168C)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.07||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC28(YBR160W)|FD-Score:-3.28|P-value:5.13E-4|Clearance:0||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:ERG8(YMR220W)|FD-Score:4.71|P-value:1.25E-6|Clearance:0.7||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI18(YBR004C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.06||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KAR2(YJL034W)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.05||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KRE5(YOR336W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.02||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:MEX67(YPL169C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MIF2(YKL089W)|FD-Score:3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:PGI1(YBR196C)|FD-Score:-3.85|P-value:6.03E-5|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RER2(YBR002C)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.04||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIO2(YNL207W)|FD-Score:-3.88|P-value:5.32E-5|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RNR2(YJL026W)|FD-Score:4|P-value:3.12E-5|Clearance:0.05||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC10(YHR143W-A)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPC34(YNR003C)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.27||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RPL10(YLR075W)|FD-Score:3.42|P-value:3.15E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL33A(YPL143W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:SEC17(YBL050W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.08||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SEN2(YLR105C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.03||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:TFA2(YKR062W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.1||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:YJU2(YKL095W)|FD-Score:-3.2|P-value:6.79E-4|Clearance:0||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YLR076C(YLR076C_d)|FD-Score:3.95|P-value:3.92E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR198C(YLR198C_d)|FD-Score:-4.81|P-value:7.57E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:ARP9(YMR033W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB3(YKL088W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.03||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CCA1(YER168C)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.07||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC28(YBR160W)|FD-Score:-3.28|P-value:5.13E-4|Clearance:0||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:ERG8(YMR220W)|FD-Score:4.71|P-value:1.25E-6|Clearance:0.7||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI18(YBR004C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.06||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KAR2(YJL034W)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.05||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KRE5(YOR336W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.02||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:MEX67(YPL169C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MIF2(YKL089W)|FD-Score:3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:PGI1(YBR196C)|FD-Score:-3.85|P-value:6.03E-5|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RER2(YBR002C)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.04||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIO2(YNL207W)|FD-Score:-3.88|P-value:5.32E-5|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RNR2(YJL026W)|FD-Score:4|P-value:3.12E-5|Clearance:0.05||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC10(YHR143W-A)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPC34(YNR003C)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.27||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RPL10(YLR075W)|FD-Score:3.42|P-value:3.15E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL33A(YPL143W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:SEC17(YBL050W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.08||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SEN2(YLR105C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.03||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:TFA2(YKR062W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.1||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:YJU2(YKL095W)|FD-Score:-3.2|P-value:6.79E-4|Clearance:0||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YLR076C(YLR076C_d)|FD-Score:3.95|P-value:3.92E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR198C(YLR198C_d)|FD-Score:-4.81|P-value:7.57E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 804
Download HOP data (tab-delimited text)  (excel)
Gene:AHP1(YLR109W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:ALD6(YPL061W)|FD-Score:5.84|P-value:2.63E-9||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALG12(YNR030W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ATG12(YBR217W)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BPH1(YCR032W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD19(YJL188C_d)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD21(YOR078W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:COA3(YJL062W-A)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COS111(YBR203W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CWH43(YCR017C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DIE2(YGR227W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:EGD1(YPL037C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELP6(YMR312W)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FAU1(YER183C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:HAL1(YPR005C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HCS1(YKL017C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HSP31(YDR533C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:IDP3(YNL009W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IOC2(YLR095C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC16(YPR038W_d)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KIN2(YLR096W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LIA1(YJR070C)|FD-Score:5.11|P-value:1.61E-7||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LIP5(YOR196C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LNP1(YHR192W_p)|FD-Score:-4.27|P-value:9.56E-6||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MST1(YKL194C)|FD-Score:7.74|P-value:4.86E-15||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NAT1(YDL040C)|FD-Score:-4.7|P-value:1.32E-6||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NMD2(YHR077C)|FD-Score:5.95|P-value:1.34E-9||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NUP42(YDR192C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:NUP60(YAR002W)|FD-Score:12.8|P-value:1.07E-37||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:NYV1(YLR093C)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:PAC2(YER007W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAD1(YDR538W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PDR15(YDR406W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PER33(YLR064W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMU1(YKL128C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PST2(YDR032C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC3(YBL056W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL19A(YBR084C-A)|FD-Score:5.36|P-value:4.19E-8||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:SAC7(YDR389W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAF1(YBR280C)|FD-Score:-4.64|P-value:1.78E-6||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAM37(YMR060C)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SGF29(YCL010C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SMA1(YPL027W)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SST2(YLR452C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:THI74(YDR438W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TVP18(YMR071C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:TYE7(YOR344C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UPS3(YDR185C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VMA1(YDL185W)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:YBR284W(YBR284W_p)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR114C(YDR114C_p)|FD-Score:5.35|P-value:4.44E-8||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR246W-A(YDR246W-A_p)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR391C(YDR391C_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YFL034W(YFL034W_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YHL015W-A(YHL015W-A_p)|FD-Score:5.27|P-value:6.93E-8||SGD DESC:Putative protein of unknown function Gene:YIR044C(YIR044C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL120W(YJL120W_d)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL182C(YJL182C_d)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJR084W(YJR084W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL031W(YKL031W_d)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL069W(YKL069W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLF2(YHL014C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL043C(YNL043C_d)|FD-Score:7.92|P-value:1.15E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YPL108W(YPL108W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL260W(YPL260W_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AHP1(YLR109W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:ALD6(YPL061W)|FD-Score:5.84|P-value:2.63E-9||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALG12(YNR030W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ATG12(YBR217W)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BPH1(YCR032W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD19(YJL188C_d)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD21(YOR078W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:COA3(YJL062W-A)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:COS111(YBR203W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CWH43(YCR017C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DIE2(YGR227W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:EGD1(YPL037C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELP6(YMR312W)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FAU1(YER183C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:HAL1(YPR005C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HCS1(YKL017C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HSP31(YDR533C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:IDP3(YNL009W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IOC2(YLR095C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC16(YPR038W_d)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KIN2(YLR096W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LIA1(YJR070C)|FD-Score:5.11|P-value:1.61E-7||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LIP5(YOR196C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LNP1(YHR192W_p)|FD-Score:-4.27|P-value:9.56E-6||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MST1(YKL194C)|FD-Score:7.74|P-value:4.86E-15||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NAT1(YDL040C)|FD-Score:-4.7|P-value:1.32E-6||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NMD2(YHR077C)|FD-Score:5.95|P-value:1.34E-9||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NUP42(YDR192C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:NUP60(YAR002W)|FD-Score:12.8|P-value:1.07E-37||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:NYV1(YLR093C)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:PAC2(YER007W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAD1(YDR538W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PDR15(YDR406W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PER33(YLR064W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMU1(YKL128C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PST2(YDR032C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC3(YBL056W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL19A(YBR084C-A)|FD-Score:5.36|P-value:4.19E-8||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPP1A(YDL081C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component Gene:SAC7(YDR389W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAF1(YBR280C)|FD-Score:-4.64|P-value:1.78E-6||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAM37(YMR060C)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SGF29(YCL010C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SMA1(YPL027W)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SST2(YLR452C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:THI74(YDR438W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TVP18(YMR071C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:TYE7(YOR344C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UPS3(YDR185C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VMA1(YDL185W)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:YBR284W(YBR284W_p)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR114C(YDR114C_p)|FD-Score:5.35|P-value:4.44E-8||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR246W-A(YDR246W-A_p)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR391C(YDR391C_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YFL034W(YFL034W_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YHL015W-A(YHL015W-A_p)|FD-Score:5.27|P-value:6.93E-8||SGD DESC:Putative protein of unknown function Gene:YIR044C(YIR044C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL120W(YJL120W_d)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL182C(YJL182C_d)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJR084W(YJR084W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL031W(YKL031W_d)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL069W(YKL069W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLF2(YHL014C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL043C(YNL043C_d)|FD-Score:7.92|P-value:1.15E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YPL108W(YPL108W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL260W(YPL260W_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR220W4.711.25E-60.70ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YJL026W4.003.12E-50.05RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YLR076C_d3.953.92E-50.01YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YNR003C3.944.11E-50.27RPC34RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
YBL050W3.671.23E-40.08SEC17Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
YER168C3.581.71E-40.07CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YKR062W3.512.21E-40.10TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YLR075W3.423.15E-40.04RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YPL143W3.383.67E-40.00RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YMR033W3.373.73E-40.01ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR336W3.363.86E-40.02KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YBR004C3.354.09E-40.06GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YLR105C3.285.15E-40.03SEN2Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
YBR002C3.255.75E-40.04RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKL089W3.216.61E-42.51E-4MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YAR002W12.801.07E-37NUP60FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153
YNL043C_d7.921.15E-15YNL043C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
YKL194C7.744.86E-15MST1Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
YHR077C5.951.34E-9NMD2Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
YPL061W5.842.63E-9ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YBR084C-A5.364.19E-8RPL19ARibosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
YDR114C_p5.354.44E-8YDR114C_pPutative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
YHL015W-A_p5.276.93E-8YHL015W-A_pPutative protein of unknown function
YJR070C5.111.61E-7LIA1Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
YCR032W5.071.97E-7BPH1Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
YKL031W_d5.062.06E-7YKL031W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
YPL260W_p4.641.72E-6YPL260W_pPutative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
YMR060C4.621.88E-6SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YLR093C4.298.85E-6NYV1v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane
YHL014C4.241.14E-5YLF2Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_1528
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0655.00E-7SGTC_3861509-0011 3.1 μMChemDiv (Drug-like library)31072800.0555556
0.0641.02E-6SGTC_20295175081 200.0 μMChembridge (Fragment library)13808760.0615385
0.0621.66E-6SGTC_2729fluphenazine 10.7 μMMiscellaneous33720.0609756
0.0621.99E-6SGTC_22206782180 200.0 μMChembridge (Fragment library)8338030.101449RNA processing & uracil transport
0.0612.63E-6SGTC_14974476-2978 41.3 μMChemDiv (Drug-like library)15283790.0394737
0.0596.17E-6SGTC_9651291-5240 308.0 μMChemDiv (Drug-like library)28345980.0581395
0.0597.43E-6SGTC_24215690543 90.7 μMChembridge (Fragment library)33979930.0519481
0.0552.38E-5SGTC_23196033012 152.6 μMChembridge (Fragment library)8472460.0714286tubulin folding & SWR complex
0.0534.15E-5SGTC_2515chrysin 20.8 μMMicrosource (Natural product library)52816070.0869565RPP1 & pyrimidine depletion
0.0534.29E-5SGTC_1807st050092 58.9 μMTimTec (Natural product derivative library)50084960.067567660S ribosome export
0.0525.88E-5SGTC_200481-0013 4.9 μMChemDiv (Drug-like library)1820450.0833333
0.0526.57E-5SGTC_14544239-0054 42.6 μMChemDiv (Drug-like library)13407670.0449438
0.0526.67E-5SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.0724638superoxide
0.0527.17E-5SGTC_1516st013856 110.0 μMTimTec (Pure natural product library)52814040.0615385
0.0527.22E-5SGTC_27927535235 81.8 μMChembridge (Drug-like library)12818910.0617284fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1540guanosine70.6 μM0.5357146802TimTec (Pure natural product library)283.24072-2.3856
SGTC_1539benzyladenosine56 μM0.37878892208TimTec (Pure natural product library)357.363860.25848
SGTC_15346-azauridine81.6 μM0.322034233502TimTec (Pure natural product library)245.18944-2.43247RNA pol III & RNase P/MRP
SGTC_2615-fluorouridine15.38 μM0.2950821821Miscellaneous262.191843-2.54147exosome
SGTC_1956st07708554 μM0.2297324208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.22368414077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes
SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid61.9 μM0.22222266535TimTec (Pure natural product library)323.196522-2.46858
SGTC_1569carminic acid40.6 μM0.22077914749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2490carminic acid95.39 μM0.22077914749TimTec (Pure natural product library)492.3864-0.436913
SGTC_1879gemcitabine100 μM0.22058860750Miscellaneous263.198146-1.39436
SGTC_248streptozotocin5.23 mM0.218755300Miscellaneous265.2206-2.28558DNA damage response