4077-0274

9-chloro-5-(4-fluorophenyl)-2-thiophen-2-yl-5,10b-dihydro-1H-pyrazolo[1,5-c][1,3]benzoxazine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_153
Screen concentration 519.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 3121976
SMILES C1C2C3=C(C=CC(=C3)Cl)OC(N2N=C1C4=CC=CS4)C5=CC=C(C=C5)F
Standardized SMILES Fc1ccc(cc1)C2Oc3ccc(Cl)cc3C4CC(=NN24)c5cccs5
Molecular weight 384.8544
ALogP 5.5
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.38
% growth inhibition (Hom. pool) 10.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3121976
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.17||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC43(YGL155W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.04||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DBF4(YDR052C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DPB11(YJL090C)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ECO1(YFR027W)|FD-Score:3.97|P-value:3.56E-5|Clearance:0.1||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD2(YGR083C)|FD-Score:7.15|P-value:4.19E-13|Clearance:0.96||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HIP1(YGR191W)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HSH49(YOR319W)|FD-Score:-5.18|P-value:1.13E-7|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV5(YLR355C)|FD-Score:5.44|P-value:2.70E-8|Clearance:0.83||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MTW1(YAL034W-A)|FD-Score:4.33|P-value:7.34E-6|Clearance:0.36||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:PGI1(YBR196C)|FD-Score:-3.1|P-value:9.54E-4|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PMA1(YGL008C)|FD-Score:3.18|P-value:7.32E-4|Clearance:0.04||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RIB2(YOL066C)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.03||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPF2(YKR081C)|FD-Score:3.14|P-value:8.49E-4|Clearance:0.09||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RRP15(YPR143W)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.02||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP43(YCR035C)|FD-Score:-4.16|P-value:1.62E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSA4(YCR072C)|FD-Score:3.78|P-value:7.76E-5|Clearance:0.29||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SEC31(YDL195W)|FD-Score:4.61|P-value:2.02E-6|Clearance:0.28||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TAM41(YGR046W)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:YCR013C(YCR013C_d)|FD-Score:3.84|P-value:6.17E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YJL195C(YJL195C_d)|FD-Score:3.88|P-value:5.32E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YKL036C(YKL036C_d)|FD-Score:3.2|P-value:6.90E-4|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YOR102W(YOR102W_d)|FD-Score:-5.59|P-value:1.10E-8|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:YRB2(YIL063C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.04||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:ZIM17(YNL310C)|FD-Score:6.2|P-value:2.83E-10|Clearance:0.76||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:CCT2(YIL142W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.17||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC43(YGL155W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.04||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DBF4(YDR052C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DPB11(YJL090C)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ECO1(YFR027W)|FD-Score:3.97|P-value:3.56E-5|Clearance:0.1||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD2(YGR083C)|FD-Score:7.15|P-value:4.19E-13|Clearance:0.96||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HIP1(YGR191W)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HSH49(YOR319W)|FD-Score:-5.18|P-value:1.13E-7|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV5(YLR355C)|FD-Score:5.44|P-value:2.70E-8|Clearance:0.83||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MTW1(YAL034W-A)|FD-Score:4.33|P-value:7.34E-6|Clearance:0.36||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:PGI1(YBR196C)|FD-Score:-3.1|P-value:9.54E-4|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PMA1(YGL008C)|FD-Score:3.18|P-value:7.32E-4|Clearance:0.04||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RIB2(YOL066C)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.03||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPF2(YKR081C)|FD-Score:3.14|P-value:8.49E-4|Clearance:0.09||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RRP15(YPR143W)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.02||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP43(YCR035C)|FD-Score:-4.16|P-value:1.62E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSA4(YCR072C)|FD-Score:3.78|P-value:7.76E-5|Clearance:0.29||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SEC31(YDL195W)|FD-Score:4.61|P-value:2.02E-6|Clearance:0.28||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TAM41(YGR046W)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:YCR013C(YCR013C_d)|FD-Score:3.84|P-value:6.17E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YJL195C(YJL195C_d)|FD-Score:3.88|P-value:5.32E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YKL036C(YKL036C_d)|FD-Score:3.2|P-value:6.90E-4|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YOR102W(YOR102W_d)|FD-Score:-5.59|P-value:1.10E-8|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:YRB2(YIL063C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.04||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:ZIM17(YNL310C)|FD-Score:6.2|P-value:2.83E-10|Clearance:0.76||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3121976
Download HOP data (tab-delimited text)  (excel)
Gene:ADE5,7(YGL234W)|FD-Score:-3.92|P-value:4.42E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ARR3(YPR201W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:AST2(YER101C)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATG36(YJL185C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:AVT5(YBL089W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BRP1(YGL007W_d)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CIR2(YOR356W)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CKA1(YIL035C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLN3(YAL040C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COX7(YMR256C)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:ECM31(YBR176W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EHT1(YBR177C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ENV9(YOR246C)|FD-Score:8.19|P-value:1.32E-16||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERJ5(YFR041C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAR7(YFR008W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FOX2(YKR009C)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:INO2(YDR123C)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:ISF1(YMR081C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LEO1(YOR123C)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MDL2(YPL270W)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEP1(YGR121C)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MGA2(YIR033W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MRS1(YIR021W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSH1(YHR120W)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NNF2(YGR089W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PAR32(YDL173W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PHO88(YBR106W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PHO90(YJL198W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PKR1(YMR123W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMT3(YOR321W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PRM5(YIL117C)|FD-Score:8.32|P-value:4.36E-17||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTK2(YJR059W)|FD-Score:4.8|P-value:7.85E-7||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD1(YPL022W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD18(YCR066W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAV1(YJR033C)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:6.56|P-value:2.63E-11||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:ROM2(YLR371W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL21B(YPL079W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPN4(YDL020C)|FD-Score:8.04|P-value:4.60E-16||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT102(YGR275W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAS3(YBL052C)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SDS3(YIL084C)|FD-Score:4.82|P-value:7.24E-7||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SFB3(YHR098C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHB17(YKR043C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIS2(YKR072C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLM6(YBR266C_d)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SPE3(YPR069C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SSA3(YBL075C)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TGL4(YKR089C)|FD-Score:-3.84|P-value:6.28E-5||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:URA1(YKL216W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VMR1(YHL035C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS15(YBR097W)|FD-Score:-4.32|P-value:7.63E-6||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:WSS1(YHR134W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR023C(YAR023C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Putative protein of unknown function Gene:YCL022C(YCL022C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDL062W(YDL062W_d)|FD-Score:-3.76|P-value:8.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDR149C(YDR149C_d)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR203W(YDR203W_d)|FD-Score:5.03|P-value:2.41E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR039C(YFR039C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGR054W(YGR054W)|FD-Score:-3.83|P-value:6.52E-5||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YKL077W(YKL077W_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKR051W(YKR051W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function Gene:YLR406C-A(YLR406C-A_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNR029C(YNR029C_p)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:ADE5,7(YGL234W)|FD-Score:-3.92|P-value:4.42E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ARR3(YPR201W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:AST2(YER101C)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATG36(YJL185C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:AVT5(YBL089W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BRP1(YGL007W_d)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CIR2(YOR356W)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:CKA1(YIL035C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLN3(YAL040C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COX7(YMR256C)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:ECM31(YBR176W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EHT1(YBR177C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ENV9(YOR246C)|FD-Score:8.19|P-value:1.32E-16||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERJ5(YFR041C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAR7(YFR008W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FOX2(YKR009C)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:INO2(YDR123C)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:ISF1(YMR081C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LEO1(YOR123C)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MDL2(YPL270W)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEP1(YGR121C)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MGA2(YIR033W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MRS1(YIR021W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSH1(YHR120W)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NNF2(YGR089W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PAR32(YDL173W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PHO88(YBR106W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PHO90(YJL198W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PKR1(YMR123W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMT3(YOR321W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PRM5(YIL117C)|FD-Score:8.32|P-value:4.36E-17||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTK2(YJR059W)|FD-Score:4.8|P-value:7.85E-7||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD1(YPL022W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD18(YCR066W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAV1(YJR033C)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:6.56|P-value:2.63E-11||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:ROM2(YLR371W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL21B(YPL079W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPN4(YDL020C)|FD-Score:8.04|P-value:4.60E-16||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT102(YGR275W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAS3(YBL052C)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SDS3(YIL084C)|FD-Score:4.82|P-value:7.24E-7||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SFB3(YHR098C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHB17(YKR043C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIS2(YKR072C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLM6(YBR266C_d)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SPE3(YPR069C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SSA3(YBL075C)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TGL4(YKR089C)|FD-Score:-3.84|P-value:6.28E-5||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:URA1(YKL216W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VMR1(YHL035C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS15(YBR097W)|FD-Score:-4.32|P-value:7.63E-6||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:WSS1(YHR134W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR023C(YAR023C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Putative protein of unknown function Gene:YCL022C(YCL022C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDL062W(YDL062W_d)|FD-Score:-3.76|P-value:8.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDR149C(YDR149C_d)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR203W(YDR203W_d)|FD-Score:5.03|P-value:2.41E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR039C(YFR039C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGR054W(YGR054W)|FD-Score:-3.83|P-value:6.52E-5||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YKL077W(YKL077W_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKR051W(YKR051W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function Gene:YLR406C-A(YLR406C-A_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNR029C(YNR029C_p)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR083C7.154.19E-130.96GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YNL310C6.202.83E-100.76ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YLR355C5.442.70E-80.83ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YDL195W4.612.02E-60.28SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YAL034W-A4.337.34E-60.36MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YFR027W3.973.56E-50.10ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YJL195C_d3.885.32E-50.04YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YCR013C_d3.846.17E-50.06YCR013C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
YCR072C3.787.76E-50.29RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YIL063C3.492.39E-40.04YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
YPR143W3.452.76E-40.02RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YIL142W3.442.92E-40.17CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGL155W3.275.40E-40.04CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
YOL066C3.236.17E-40.03RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YKL036C_d3.206.90E-40.02YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL117C8.324.36E-17PRM5Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling
YOR246C8.191.32E-16ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YDL020C8.044.60E-16RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR202C6.562.63E-11RAV2Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
YDR203W_d5.032.41E-7YDR203W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML122C_d4.993.00E-7YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YIL084C4.827.24E-7SDS3Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
YJR059W4.807.85E-7PTK2Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
YMR123W4.711.23E-6PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YHR098C4.592.23E-6SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YGL007W_d4.523.09E-6BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YBL075C4.405.31E-6SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YCR066W4.111.98E-5RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YBL052C4.032.83E-5SAS3Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal
YER101C3.846.24E-5AST2Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_153
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2114.33E-60SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.373134
0.1783.85E-43SGTC_204PMA1.184 62.4 μMChemDiv (Drug-like library)22522130.0869565TRP & mitochondrial translation
0.1301.34E-23SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.111111
0.1293.25E-23SGTC_2248tofa 1.1 μMMiscellaneous1151750.0493827sphingolipid biosynthesis & PDR1
0.1255.37E-22SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0422535
0.1241.41E-21SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.0617284sphingolipid biosynthesis & PDR1
0.1171.86E-19SGTC_16014'-hydroxyflavanone 55.5 μMTimTec (Pure natural product library)1655060.166667RPP1 & pyrimidine depletion
0.1164.05E-19SGTC_1420335-0849 62.7 μMChemDiv (Drug-like library)38890430.0795455ubiquinone biosynthesis & proteasome
0.1141.59E-18SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0519481
0.1095.37E-17SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0675676
0.1072.02E-16SGTC_315k915-0449 187.0 μMChemDiv (Drug-like library)209702150.114583
0.1056.54E-16SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 150.0 μMICCB bioactive library23740.046875Golgi
0.1024.23E-15SGTC_2695st077686 52.0 μMTimTec (Natural product derivative library)28760920.0808081
0.1016.95E-15SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0519481
0.0961.22E-13SGTC_29679089538 49.9 μMChembridge (Drug-like library)7282160.108434

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3354077-025627.6 μM0.4142863121989ChemDiv (Drug-like library)363.27213.69404
SGTC_2094077-0173175.59 μM0.37313415993494ChemDiv (Drug-like library)284.740223.53903
SGTC_3364111-0029111 μM0.3714293131175ChemDiv (Drug-like library)327.379183.52504
SGTC_1780st03844824.54 μM0.304348728868TimTec (Natural product derivative library)278.710883.21614iron homeostasis
SGTC_687k029-004434.9 μM0.243595829338ChemDiv (Drug-like library)337.2274.59214PDR1
SGTC_1799st04582246.14 μM0.2134833674992TimTec (Natural product derivative library)349.42792.42606
SGTC_1743553-038974.3 μM0.208333738071ChemDiv (Drug-like library)287.78563.25414
SGTC_2731amoxapine43.43 μM0.2077922170Miscellaneous313.781442.89713
SGTC_1781st03872566.3 μM0.2054794270275TimTec (Natural product derivative library)301.767443.50413NEO1
SGTC_10184259-001328.1 μM0.2025326801262ChemDiv (Drug-like library)318.75491.44935