6-azauridine

2-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2,4-triazine-3,5-dione

6-Azauridine (AzUrd) blocks the conversion of orotic acid into UMP; used in antiviral studies.

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PubChem MeSH terms: Antimetabolites, Antineoplastic;Dermatologic Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1534
Screen concentration 81.6 μM
Source TimTec (Pure natural product library)
PubChem CID 233502
SMILES C1=NN(C(=O)NC1=O)C2C(C(C(O2)CO)O)O
Standardized SMILES OCC1OC(C(O)C1O)N2N=CC(=O)NC2=O
Molecular weight 245.1894
ALogP -2.43
H-bond donor count 4
H-bond acceptor count 7
Response signature RNA pol III & RNase P/MRP

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.61
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 233502
Download HIP data (tab-delimited text)  (excel)
Gene:BET5(YML077W)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CDC11(YJR076C)|FD-Score:-3.25|P-value:5.78E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC31(YOR257W)|FD-Score:5.58|P-value:1.20E-8|Clearance:0.97||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:GLN4(YOR168W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.08||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:GPI18(YBR004C)|FD-Score:4.61|P-value:2.06E-6|Clearance:0.29||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:PDS1(YDR113C)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.03||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:POP1(YNL221C)|FD-Score:8.11|P-value:2.44E-16|Clearance:2.53||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP4(YBR257W)|FD-Score:4.31|P-value:8.11E-6|Clearance:0.2||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RAT1(YOR048C)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.01||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RET1(YOR207C)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.45||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPA190(YOR341W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.05||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPC31(YNL151C)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RPC53(YDL150W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.11||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:3.12|P-value:8.94E-4|Clearance:0.09||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:15.4|P-value:6.02E-54|Clearance:7.31||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT3(YDR394W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RRP4(YHR069C)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC8(YFR037C)|FD-Score:3.43|P-value:3.04E-4|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:TIF6(YPR016C)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRE2(YOR256C)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.09||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YJL086C(YJL086C_d)|FD-Score:-3.32|P-value:4.56E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:BET5(YML077W)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CDC11(YJR076C)|FD-Score:-3.25|P-value:5.78E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC31(YOR257W)|FD-Score:5.58|P-value:1.20E-8|Clearance:0.97||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:GLN4(YOR168W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.08||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:GPI18(YBR004C)|FD-Score:4.61|P-value:2.06E-6|Clearance:0.29||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:PDS1(YDR113C)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.03||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:POP1(YNL221C)|FD-Score:8.11|P-value:2.44E-16|Clearance:2.53||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP4(YBR257W)|FD-Score:4.31|P-value:8.11E-6|Clearance:0.2||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RAT1(YOR048C)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.01||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RET1(YOR207C)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.45||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPA190(YOR341W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.05||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPC31(YNL151C)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RPC53(YDL150W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.11||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:3.12|P-value:8.94E-4|Clearance:0.09||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:15.4|P-value:6.02E-54|Clearance:7.31||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT3(YDR394W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RRP4(YHR069C)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC8(YFR037C)|FD-Score:3.43|P-value:3.04E-4|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:TIF6(YPR016C)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRE2(YOR256C)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.09||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YJL086C(YJL086C_d)|FD-Score:-3.32|P-value:4.56E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 233502
Download HOP data (tab-delimited text)  (excel)
Gene:AMD1(YML035C)|FD-Score:7.46|P-value:4.40E-14||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ARD1(YHR013C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:BSC2(YDR275W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:CCP1(YKR066C)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CUP9(YPL177C)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:CWC21(YDR482C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DAL82(YNL314W)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DAS1(YJL149W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DDC1(YPL194W)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:EAP1(YKL204W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EIS1(YMR031C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FMP21(YBR269C_p)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUM1(YPL262W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCV3(YAL044C)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GET1(YGL020C)|FD-Score:-3.8|P-value:7.25E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:IES3(YLR052W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IES6(YEL044W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRC7(YFR055W_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:KAP122(YGL016W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:KRE1(YNL322C)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LRS4(YDR439W)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAP1(YLR244C)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCT1(YOR221C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM34(YGL219C)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MFA1(YDR461W)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MNN2(YBR015C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRN1(YPL184C)|FD-Score:4.28|P-value:9.25E-6||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRPL15(YLR312W-A)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB4(YOL112W)|FD-Score:5.49|P-value:2.06E-8||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSS18(YPR134W)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MXR1(YER042W)|FD-Score:7.78|P-value:3.52E-15||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAT1(YDL040C)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NHP6A(YPR052C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUM1(YDR150W)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCT1(YKL134C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PGC1(YPL206C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO23(YNL097C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PKH3(YDR466W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMP3(YDR276C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PSK2(YOL045W)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PUS7(YOR243C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RML2(YEL050C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROT2(YBR229C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:ROX3(YBL093C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL13B(YMR142C)|FD-Score:6.65|P-value:1.44E-11||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL7A(YGL076C)|FD-Score:6.71|P-value:9.62E-12||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SCO1(YBR037C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SDH1(YKL148C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SET4(YJL105W)|FD-Score:7.3|P-value:1.41E-13||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SKI3(YPR189W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLM3(YDL033C)|FD-Score:5.06|P-value:2.13E-7||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLP1(YOR154W_p)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLX5(YDL013W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SLZ1(YNL196C_p)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNC1(YAL030W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNF4(YGL115W)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SUR1(YPL057C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:UBP3(YER151C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:6.39|P-value:8.30E-11||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS9(YML097C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YCR007C(YCR007C_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR022C(YCR022C_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:6.25|P-value:2.06E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHL045W(YHL045W_d)|FD-Score:4.35|P-value:6.66E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL100W(YIL100W_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YJL182C(YJL182C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJR015W(YJR015W_p)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKL027W(YKL027W)|FD-Score:4.36|P-value:6.46E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL147C(YKL147C_d)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YOR041C(YOR041C_d)|FD-Score:-4.1|P-value:2.05E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR052C(YOR052C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR097C(YOR097C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL136W(YPL136W_d)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR039W(YPR039W_d)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:AMD1(YML035C)|FD-Score:7.46|P-value:4.40E-14||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ARD1(YHR013C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:BSC2(YDR275W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:CCP1(YKR066C)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CUP9(YPL177C)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:CWC21(YDR482C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DAL82(YNL314W)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DAS1(YJL149W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DDC1(YPL194W)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:EAP1(YKL204W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EIS1(YMR031C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FMP21(YBR269C_p)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUM1(YPL262W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCV3(YAL044C)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GET1(YGL020C)|FD-Score:-3.8|P-value:7.25E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:IES3(YLR052W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IES6(YEL044W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRC7(YFR055W_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:KAP122(YGL016W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:KRE1(YNL322C)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LRS4(YDR439W)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAP1(YLR244C)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCT1(YOR221C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM34(YGL219C)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MFA1(YDR461W)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MNN2(YBR015C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRN1(YPL184C)|FD-Score:4.28|P-value:9.25E-6||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRPL15(YLR312W-A)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB4(YOL112W)|FD-Score:5.49|P-value:2.06E-8||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSS18(YPR134W)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MXR1(YER042W)|FD-Score:7.78|P-value:3.52E-15||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAT1(YDL040C)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NHP6A(YPR052C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUM1(YDR150W)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCT1(YKL134C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PGC1(YPL206C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO23(YNL097C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PKH3(YDR466W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMP3(YDR276C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PSK2(YOL045W)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PUS7(YOR243C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RML2(YEL050C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROT2(YBR229C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:ROX3(YBL093C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL13B(YMR142C)|FD-Score:6.65|P-value:1.44E-11||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL7A(YGL076C)|FD-Score:6.71|P-value:9.62E-12||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SCO1(YBR037C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SDH1(YKL148C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SET4(YJL105W)|FD-Score:7.3|P-value:1.41E-13||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SKI3(YPR189W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLM3(YDL033C)|FD-Score:5.06|P-value:2.13E-7||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLP1(YOR154W_p)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLX5(YDL013W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SLZ1(YNL196C_p)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNC1(YAL030W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNF4(YGL115W)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SUR1(YPL057C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:UBP3(YER151C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:6.39|P-value:8.30E-11||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS9(YML097C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YCR007C(YCR007C_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR022C(YCR022C_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:6.25|P-value:2.06E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHL045W(YHL045W_d)|FD-Score:4.35|P-value:6.66E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL100W(YIL100W_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YJL182C(YJL182C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJR015W(YJR015W_p)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKL027W(YKL027W)|FD-Score:4.36|P-value:6.46E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL147C(YKL147C_d)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YOR041C(YOR041C_d)|FD-Score:-4.1|P-value:2.05E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR052C(YOR052C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR097C(YOR097C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL136W(YPL136W_d)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR039W(YPR039W_d)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C15.406.02E-547.31RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNL221C8.112.44E-162.53POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YOR257W5.581.20E-80.97CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YBR004C4.612.06E-60.29GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YBR257W4.318.11E-60.20POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YPR016C4.111.96E-50.08TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YOR207C4.032.74E-50.45RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YOR341W3.591.68E-40.05RPA190RNA polymerase I largest subunit A190
YDL150W3.542.01E-40.11RPC53RNA polymerase III subunit C53
YFR037C3.433.04E-40.06RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDR394W3.373.72E-40.04RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YOR048C3.334.33E-40.01RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YDR113C3.324.45E-40.03PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YOR168W3.304.90E-40.08GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YOR256C3.226.48E-40.09TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER042W7.783.52E-15MXR1Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan
YML035C7.464.40E-14AMD1AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
YJL105W7.301.41E-13SET4Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
YGL076C6.719.62E-12RPL7ARibosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication
YMR142C6.651.44E-11RPL13BRibosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
YDR495C6.398.30E-11VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YGR290W_d6.252.06E-10YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YOL112W5.492.06E-8MSB4GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization
YDL033C5.062.13E-7SLM3tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
YOR243C4.621.96E-6PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YDR150W4.552.64E-6NUM1Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
YAL044C4.405.32E-6GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YLR244C4.386.05E-6MAP1Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
YKL027W4.366.46E-6YKL027WProtein of unknown function, localized to the mitochondrial outer membrane
YHL045W_d4.356.66E-6YHL045W_dPutative protein of unknown function; not an essential gene

GO enrichment analysis for SGTC_1534
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2281.75E-70SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.0769231RNA pol III & RNase P/MRP
0.2181.99E-64SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.0943396RNA pol III & RNase P/MRP
0.2091.64E-59SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0769231RPP1 & pyrimidine depletion
0.1998.88E-54SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)29016130.0740741RNA pol III & RNase P/MRP
0.1931.61E-50SGTC_919tnp00227 403.6 nMTimTec (Natural product library)12692460.0641026RNA pol III & RNase P/MRP
0.1742.24E-41SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0675676RNA pol III & RNase P/MRP
0.1736.98E-41SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.08RPP1 & pyrimidine depletion
0.1578.77E-34SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.0526316RNA pol III & RNase P/MRP
0.1527.86E-32SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.0714286
0.1512.69E-31SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.0757576RPP1 & pyrimidine depletion
0.1505.91E-31SGTC_1642st009582 77.7 μMTimTec (Natural product derivative library)67393110.0606061RPP1 & pyrimidine depletion
0.1446.44E-29SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.0909091copper-dependent oxidative stress
0.1441.02E-28SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.1441.35E-28SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.0645161RNA pol III & RNase P/MRP
0.1425.59E-28SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0779221

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2615-fluorouridine15.38 μM0.4897961821Miscellaneous262.191843-2.54147exosome
SGTC_1540guanosine70.6 μM0.3333336802TimTec (Pure natural product library)283.24072-2.3856
SGTC_1528arabinosylhypoxanthine74.6 μM0.322034804TimTec (Pure natural product library)268.22608-2.24946
SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid61.9 μM0.30158766535TimTec (Pure natural product library)323.196522-2.46858
SGTC_1789tunicamycin25 nM0.28735623847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_255tunicamycin200.47 nM0.28735623847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_1879gemcitabine100 μM0.26229560750Miscellaneous263.198146-1.39436
SGTC_1956st07708554 μM0.2524208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.24285714077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes
SGTC_248streptozotocin5.23 mM0.2413795300Miscellaneous265.2206-2.28558DNA damage response
SGTC_1569carminic acid40.6 μM0.23943714749TimTec (Pure natural product library)492.3864-0.436913