tetrahydropapaveroline hydrobromide

1-[(3,4-dihydroxyphenyl)methyl]-1,2,3,4-tetrahydroisoquinoline-6,7-diol hydrobromide

An inhibitor of dopamine biosynthesis.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1535
Screen concentration 54.3 μM
Source TimTec (Pure natural product library)
PubChem CID 2724278
SMILES C1CNC(C2=CC(=C(C=C21)O)O)CC3=CC(=C(C=C3)O)O.Br
Standardized SMILES Oc1ccc(CC2NCCc3cc(O)c(O)cc23)cc1O
Molecular weight 368.2224
ALogP 2.9
H-bond donor count 5
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.14
% growth inhibition (Hom. pool) -2.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2724278
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.29||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:DFR1(YOR236W)|FD-Score:4.1|P-value:2.09E-5|Clearance:0.52||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:GLC7(YER133W)|FD-Score:4|P-value:3.19E-5|Clearance:0.52||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:RLP24(YLR009W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.02||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC17(YJL011C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.17||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:YDL221W(YDL221W_d)|FD-Score:4.09|P-value:2.19E-5|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:ARP4(YJL081C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.29||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:DFR1(YOR236W)|FD-Score:4.1|P-value:2.09E-5|Clearance:0.52||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:GLC7(YER133W)|FD-Score:4|P-value:3.19E-5|Clearance:0.52||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:RLP24(YLR009W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.02||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC17(YJL011C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.17||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:YDL221W(YDL221W_d)|FD-Score:4.09|P-value:2.19E-5|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2724278
Download HOP data (tab-delimited text)  (excel)
Gene:ALK2(YBL009W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATG26(YLR189C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BAR1(YIL015W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:CKA2(YOR061W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COX16(YJL003W)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:COX5B(YIL111W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication Gene:CYB2(YML054C)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DNL4(YOR005C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:FUS2(YMR232W)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:HAL9(YOL089C)|FD-Score:6.23|P-value:2.30E-10||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HIS1(YER055C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IES3(YLR052W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC16(YPR038W_d)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KES1(YPL145C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LSC1(YOR142W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MDM12(YOL009C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MDM34(YGL219C)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MLH3(YPL164C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:NTH2(YBR001C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUT1(YGL151W)|FD-Score:-3.96|P-value:3.77E-5||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:PGM3(YMR278W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIF1(YML061C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RAX2(YLR084C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:ROD1(YOR018W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPA49(YNL248C)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPL22A(YLR061W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:RRP8(YDR083W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SOH1(YGL127C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SYH1(YPL105C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBC8(YEL012W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VPS63(YLR261C_d)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL070C(YBL070C_d)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR022C(YHR022C_p)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YJL064W(YJL064W_d)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YKL118W(YKL118W_d)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL136W(YKL136W_d)|FD-Score:-3.93|P-value:4.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR428C(YLR428C_d)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YML002W(YML002W_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR027W(YMR027W_p)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL179C(YNL179C_d)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YPL261C(YPL261C_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:ALK2(YBL009W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATG26(YLR189C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BAR1(YIL015W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:CKA2(YOR061W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COX16(YJL003W)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:COX5B(YIL111W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication Gene:CYB2(YML054C)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DNL4(YOR005C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:FUS2(YMR232W)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:HAL9(YOL089C)|FD-Score:6.23|P-value:2.30E-10||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HIS1(YER055C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IES3(YLR052W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC16(YPR038W_d)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KES1(YPL145C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LSC1(YOR142W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MDM12(YOL009C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MDM34(YGL219C)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MLH3(YPL164C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:NTH2(YBR001C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUT1(YGL151W)|FD-Score:-3.96|P-value:3.77E-5||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:PGM3(YMR278W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIF1(YML061C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RAX2(YLR084C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:ROD1(YOR018W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPA49(YNL248C)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPL22A(YLR061W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:RRP8(YDR083W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SOH1(YGL127C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SYH1(YPL105C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBC8(YEL012W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VPS63(YLR261C_d)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL070C(YBL070C_d)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR022C(YHR022C_p)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YJL064W(YJL064W_d)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YKL118W(YKL118W_d)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL136W(YKL136W_d)|FD-Score:-3.93|P-value:4.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR428C(YLR428C_d)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YML002W(YML002W_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR027W(YMR027W_p)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL179C(YNL179C_d)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YPL261C(YPL261C_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR236W4.102.09E-50.52DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YDL221W_d4.092.19E-50.52YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YER133W4.003.19E-50.52GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YJL081C3.482.50E-40.29ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YLR009W3.197.13E-40.02RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YJL011C3.177.61E-40.17RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YKL210W3.000.001350.04UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YDR228C2.960.001550.09PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YGR095C2.870.002040.07RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YPR033C2.800.002530.01HTS1Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
YGL116W2.790.002610.02CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDR211W2.770.002810.30GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YHR196W2.470.006800.06UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YPL128C2.410.007910.01TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YOL021C2.400.008210.10DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL089C6.232.30E-10HAL9Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
YEL012W4.641.75E-6UBC8Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
YJL003W4.612.05E-6COX16Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
YKL118W_d4.425.04E-6YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YLR261C_d4.251.08E-5VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YOR018W4.191.37E-5ROD1Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication
YLR052W4.151.65E-5IES3Subunit of the INO80 chromatin remodeling complex
YJL064W_d4.072.35E-5YJL064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1
YPR038W_d4.072.36E-5IRC16_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci
YBR001C3.983.42E-5NTH2Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication
YMR278W3.963.69E-5PGM3Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
YNL179C_d3.934.22E-5YNL179C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
YNL248C3.904.88E-5RPA49RNA polymerase I subunit A49
YMR232W3.787.91E-5FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YIL111W3.701.08E-4COX5BSubunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1535
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0723.73E-8SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.1RNA processing & uracil transport
0.0699.95E-8SGTC_20615245884 81.0 μMChembridge (Fragment library)5738630.0491803tubulin folding & SWR complex
0.0672.81E-7SGTC_5761073-0113 22.7 μMChemDiv (Drug-like library)54395630.0740741redox potentiating
0.0654.85E-7SGTC_22047233102 200.0 μMChembridge (Fragment library)21724800.059701560S ribosome export
0.0641.00E-6SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid 61.9 μMTimTec (Pure natural product library)665350.0666667
0.0631.18E-6SGTC_2505galangin 7.9 μMMicrosource (Natural product library)52816160.116667RPP1 & pyrimidine depletion
0.0631.19E-6SGTC_2502avocadyne acetate 18.6 μMMicrosource (Natural product library)39520790.030303
0.0621.66E-6SGTC_31839108214 49.5 μMChembridge (Drug-like library)49748310.0625RPP1 & pyrimidine depletion
0.0621.94E-6SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0714286translation
0.0613.09E-6SGTC_1979st071809 45.3 μMTimTec (Natural product derivative library)49763330.1RPP1 & pyrimidine depletion
0.0595.12E-6SGTC_1470k295-0292 231.0 μMChemDiv (Drug-like library)50456120.08
0.0595.88E-6SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.0909091RSC complex & mRNA processing
0.0588.36E-6SGTC_506n9-isopropylolomoucine 153.0 μMICCB bioactive library66103550.0810811
0.0588.78E-6SGTC_32329132874 49.5 μMChembridge (Drug-like library)37849400.0655738RPP1 & pyrimidine depletion
0.0588.91E-6SGTC_32389133470 49.5 μMChembridge (Drug-like library)35346880.101695

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1808st05006361.5 μM0.3272734011993TimTec (Natural product derivative library)325.444584.66113
SGTC_470149-013524.25 μM0.2916675239155ChemDiv (Drug-like library)328.445225.87933TSC3-RPN4
SGTC_1806st05000560.3 μM0.2807025194765TimTec (Natural product derivative library)331.836484.38313
SGTC_2764methyldopa94.69 μM0.2838853Miscellaneous211.21452-1.77845
SGTC_1811st05006961.5 μM0.2758623877702TimTec (Natural product derivative library)325.444584.69113
SGTC_1781st03872566.3 μM0.2727274270275TimTec (Natural product derivative library)301.767443.50413NEO1
SGTC_1800st05007929.07 μM0.2711863645560TimTec (Natural product derivative library)373.487385.23713NEO1-PIK1
SGTC_1807st05009258.9 μM0.2678575008496TimTec (Natural product derivative library)339.471165.1781360S ribosome export
SGTC_1805st05001360.3 μM0.2666674037144TimTec (Natural product derivative library)331.836484.38313
SGTC_1809st05008157.6 μM0.2666675036894TimTec (Natural product derivative library)347.45014.62713