3389-1638

N-(3,4-dichlorophenyl)-7-methoxy-2,2,4-trimethylquinoline-1-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_154
Screen concentration 511.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 5238686
SMILES CC1=CC(N(C2=C1C=CC(=C2)OC)C(=O)NC3=CC(=C(C=C3)Cl)Cl)(C)C
Standardized SMILES COc1ccc2C(=CC(C)(C)N(C(=O)Nc3ccc(Cl)c(Cl)c3)c2c1)C
Molecular weight 391.291
ALogP 5.29
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.87
% growth inhibition (Hom. pool) 3.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5238686
Download HIP data (tab-delimited text)  (excel)
Gene:DRS1(YLL008W)|FD-Score:-3.46|P-value:2.74E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG1(YGR175C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.2||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG29(YMR134W_p)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GCD2(YGR083C)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.16||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI12(YMR281W)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.04||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:LSG1(YGL099W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MEC1(YBR136W)|FD-Score:4.62|P-value:1.93E-6|Clearance:0.34||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MVD1(YNR043W)|FD-Score:3.2|P-value:6.76E-4|Clearance:0.09||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NOP8(YOL144W)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PRE1(YER012W)|FD-Score:3.93|P-value:4.18E-5|Clearance:0.02||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRP21(YJL203W)|FD-Score:3.31|P-value:4.62E-4|Clearance:0.04||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RFA3(YJL173C)|FD-Score:-4.17|P-value:1.50E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RHO1(YPR165W)|FD-Score:3.91|P-value:4.61E-5|Clearance:0.19||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL15A(YLR029C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL18A(YOL120C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:4.42|P-value:4.87E-6|Clearance:0.34||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC39(YLR440C)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.34||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SMX3(YPR182W)|FD-Score:3.27|P-value:5.41E-4|Clearance:0.02||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:YCG1(YDR325W)|FD-Score:3.12|P-value:9.18E-4|Clearance:0.06||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:DRS1(YLL008W)|FD-Score:-3.46|P-value:2.74E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG1(YGR175C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.2||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG29(YMR134W_p)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GCD2(YGR083C)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.16||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI12(YMR281W)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.04||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:LSG1(YGL099W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MEC1(YBR136W)|FD-Score:4.62|P-value:1.93E-6|Clearance:0.34||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MVD1(YNR043W)|FD-Score:3.2|P-value:6.76E-4|Clearance:0.09||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NOP8(YOL144W)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PRE1(YER012W)|FD-Score:3.93|P-value:4.18E-5|Clearance:0.02||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRP21(YJL203W)|FD-Score:3.31|P-value:4.62E-4|Clearance:0.04||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RFA3(YJL173C)|FD-Score:-4.17|P-value:1.50E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RHO1(YPR165W)|FD-Score:3.91|P-value:4.61E-5|Clearance:0.19||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL15A(YLR029C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL18A(YOL120C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:4.42|P-value:4.87E-6|Clearance:0.34||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC39(YLR440C)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.34||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SMX3(YPR182W)|FD-Score:3.27|P-value:5.41E-4|Clearance:0.02||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:YCG1(YDR325W)|FD-Score:3.12|P-value:9.18E-4|Clearance:0.06||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5238686
Download HOP data (tab-delimited text)  (excel)
Gene:ATP7(YKL016C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT3(YKL146W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CDC26(YFR036W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLN1(YMR199W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DAL7(YIR031C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIT2(YDR402C)|FD-Score:-5.39|P-value:3.45E-8||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EDC3(YEL015W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ELM1(YKL048C)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERJ5(YFR041C)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FMP10(YER182W_p)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE4(YNR060W)|FD-Score:5.01|P-value:2.75E-7||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GCG1(YER163C_p)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GLR1(YPL091W)|FD-Score:5.2|P-value:9.90E-8||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GUT1(YHL032C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HEM14(YER014W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIS6(YIL020C)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HIT1(YJR055W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HVG1(YER039C)|FD-Score:4.34|P-value:7.08E-6||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:HXT3(YDR345C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:KRE1(YNL322C)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KTR7(YIL085C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MAL11(YGR289C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MDJ2(YNL328C)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MDS3(YGL197W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MNN2(YBR015C)|FD-Score:6.14|P-value:4.07E-10||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOH1(YBL049W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MUP3(YHL036W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:MVP1(YMR004W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NFI1(YOR156C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:OSH2(YDL019C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OXA1(YER154W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAI3(YMR174C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PCD1(YLR151C)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PET122(YER153C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PIN3(YPR154W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PPR1(YLR014C)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PTC7(YHR076W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PUS7(YOR243C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RPL23B(YER117W)|FD-Score:5.33|P-value:5.03E-8||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24B(YIL069C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:5.81|P-value:3.15E-9||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTC5(YOR118W)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:SAS2(YMR127C)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SAW1(YAL027W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SFC1(YJR095W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SGE1(YPR198W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIA1(YOR137C)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIN3(YOL004W)|FD-Score:-4.63|P-value:1.83E-6||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SOD1(YJR104C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE3(YPR069C)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPO21(YOL091W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPS100(YHR139C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSN3(YPL042C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STV1(YMR054W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUN4(YNL066W)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TRK1(YJL129C)|FD-Score:-4.9|P-value:4.73E-7||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:ULS1(YOR191W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VEL1(YGL258W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS15(YBR097W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAT1(YAR035W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative protein of unknown function Gene:YCT1(YLL055W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YEL045C(YEL045C_d)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGR021W(YGR021W_p)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR266W(YGR266W)|FD-Score:6.34|P-value:1.17E-10||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHM2(YMR241W)|FD-Score:4.91|P-value:4.65E-7||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIA6(YIL006W)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIG1(YPL201C)|FD-Score:-3.78|P-value:7.73E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YJL022W(YJL022W_d)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJL213W(YJL213W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKE4(YIL023C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL133C(YKL133C_p)|FD-Score:4.77|P-value:9.02E-7||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLF2(YHL014C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YMR209C(YMR209C_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:4.34|P-value:7.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPL088W(YPL088W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPR130C(YPR130C_d)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR195C(YPR195C_d)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ZRC1(YMR243C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ATP7(YKL016C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT3(YKL146W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CDC26(YFR036W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLN1(YMR199W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DAL7(YIR031C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIT2(YDR402C)|FD-Score:-5.39|P-value:3.45E-8||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EDC3(YEL015W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ELM1(YKL048C)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERJ5(YFR041C)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FMP10(YER182W_p)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE4(YNR060W)|FD-Score:5.01|P-value:2.75E-7||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GCG1(YER163C_p)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GLR1(YPL091W)|FD-Score:5.2|P-value:9.90E-8||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GUT1(YHL032C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HEM14(YER014W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIS6(YIL020C)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HIT1(YJR055W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HVG1(YER039C)|FD-Score:4.34|P-value:7.08E-6||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:HXT3(YDR345C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:KRE1(YNL322C)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KTR7(YIL085C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MAL11(YGR289C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MDJ2(YNL328C)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MDS3(YGL197W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MNN2(YBR015C)|FD-Score:6.14|P-value:4.07E-10||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOH1(YBL049W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MUP3(YHL036W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:MVP1(YMR004W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NFI1(YOR156C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:OSH2(YDL019C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OXA1(YER154W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAI3(YMR174C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PCD1(YLR151C)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PET122(YER153C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PIN3(YPR154W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PPR1(YLR014C)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PTC7(YHR076W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PUS7(YOR243C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RPL23B(YER117W)|FD-Score:5.33|P-value:5.03E-8||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24B(YIL069C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:5.81|P-value:3.15E-9||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTC5(YOR118W)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:SAS2(YMR127C)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SAW1(YAL027W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SFC1(YJR095W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SGE1(YPR198W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIA1(YOR137C)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIN3(YOL004W)|FD-Score:-4.63|P-value:1.83E-6||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SOD1(YJR104C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE3(YPR069C)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPO21(YOL091W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPS100(YHR139C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSN3(YPL042C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STV1(YMR054W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUN4(YNL066W)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TRK1(YJL129C)|FD-Score:-4.9|P-value:4.73E-7||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:ULS1(YOR191W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VEL1(YGL258W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS15(YBR097W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAT1(YAR035W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative protein of unknown function Gene:YCT1(YLL055W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YEL045C(YEL045C_d)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGR021W(YGR021W_p)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR266W(YGR266W)|FD-Score:6.34|P-value:1.17E-10||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHM2(YMR241W)|FD-Score:4.91|P-value:4.65E-7||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIA6(YIL006W)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIG1(YPL201C)|FD-Score:-3.78|P-value:7.73E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YJL022W(YJL022W_d)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJL213W(YJL213W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKE4(YIL023C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL133C(YKL133C_p)|FD-Score:4.77|P-value:9.02E-7||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLF2(YHL014C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YMR209C(YMR209C_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:4.34|P-value:7.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPL088W(YPL088W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPR130C(YPR130C_d)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR195C(YPR195C_d)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ZRC1(YMR243C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR440C4.681.42E-60.34SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YBR136W4.621.93E-60.34MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YDR280W4.424.87E-60.34RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YOL120C4.082.26E-50.15RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YER012W3.934.18E-50.02PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YPR165W3.914.61E-50.19RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YGR175C3.721.00E-40.20ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YGR083C3.522.17E-40.16GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGL099W3.353.97E-40.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YLR029C3.334.40E-40.01RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YJL203W3.314.62E-40.04PRP21Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YPR182W3.275.41E-40.02SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
YMR281W3.245.89E-40.04GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YNR043W3.206.76E-40.09MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YDR325W3.129.18E-40.06YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR266W6.341.17E-10YGR266WProtein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress
YBR015C6.144.07E-10MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YLR287C-A5.813.15E-9RPS30AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
YHL014C5.492.01E-8YLF2Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YER117W5.335.03E-8RPL23BRibosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
YPL091W5.209.90E-8GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YAL027W5.181.12E-7SAW15'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YLR151C5.072.02E-7PCD1Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member
YNR060W5.012.75E-7FRE4Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
YMR241W4.914.65E-7YHM2Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
YKL133C_p4.779.02E-7YKL133C_pPutative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype)
YJR104C4.631.85E-6SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YNL322C4.612.06E-6KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YER163C_p4.582.35E-6GCG1_pGamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
YJR055W4.444.42E-6HIT1Protein of unknown function, required for growth at high temperature

GO enrichment analysis for SGTC_154
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0806.06E-10SGTC_2751anetholtrithion 17.2 μMMiscellaneous21940.15625
0.0741.49E-8SGTC_1862st057513 3.8 μMTimTec (Natural product derivative library)53931630.0649351
0.0707.73E-8SGTC_8890919-1524 82.7 μMChemDiv (Drug-like library)39054520.126582
0.0707.94E-8SGTC_1483253-0744 288.0 μMChemDiv (Drug-like library)68936550.125
0.0672.46E-7SGTC_5401187-0821 17.3 μMChemDiv (Drug-like library)8475200.132353
0.0649.96E-7SGTC_33069127676 59.8 μMChembridge (Drug-like library)419052870.0487805
0.0631.16E-6SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.048780560S ribosome export
0.0631.17E-6SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.0560S ribosome export
0.0621.73E-6SGTC_1513013-0144 2.3 μMChemDiv (Drug-like library)9065580.153846iron homeostasis
0.0621.79E-6SGTC_1117retinoic acid 1.1 μMTimTec (Natural product library)4447950.0987654
0.0621.93E-6SGTC_33019125354 19.8 μMChembridge (Drug-like library)419051620.0581395
0.0604.15E-6SGTC_27927535235 81.8 μMChembridge (Drug-like library)12818910.164557fatty acid desaturase (OLE1)
0.0595.73E-6SGTC_6450845-0943 5.4 μMChemDiv (Drug-like library)18851060.26666760S ribosome export
0.0588.77E-6SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.12987RNA processing & uracil transport
0.0571.06E-5SGTC_750159-0059 199.0 μMChemDiv (Drug-like library)53772700.148649fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1823448-4371550.52 μM0.4307693491403ChemDiv (Drug-like library)363.28095.42211
SGTC_1460k062-00599.15 μM0.3384621373085ChemDiv (Drug-like library)368.2328434.31513Golgi
SGTC_8991083-0020478 μM0.338235767123ChemDiv (Drug-like library)306.701143.15714
SGTC_12961068-010211.9 μM0.333333767053ChemDiv (Drug-like library)310.175224.02712
SGTC_1270180-033359.13 μM0.328125771601ChemDiv (Drug-like library)324.158743.71113mitochondrial response to ROS
SGTC_3298911685871.43 μM0.3181822506942Chembridge (Drug-like library)279.718862.65713
SGTC_2900532852885.29 μM0.31746795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_3265913726649.47 μM0.31343317224265Chembridge (Drug-like library)357.274783.71112
SGTC_3284910817164.86 μM0.3108118687505Chembridge (Drug-like library)373.876463.7214
SGTC_2783773816571.43 μM0.3030317201110Chembridge (Drug-like library)289.756743.55612