guanosine

2-amino-9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-3H-purin-6-one

Guanosine is a purine nucleoside

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1540
Screen concentration 70.6 μM
Source TimTec (Pure natural product library)
PubChem CID 6802
SMILES C1=NC2=C(N1C3C(C(C(O3)CO)O)O)NC(=NC2=O)N
Standardized SMILES Nc1nc(O)c2ncn(C3OC(CO)C(O)C3O)c2n1
Molecular weight 283.2407
ALogP -2.38
H-bond donor count 5
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.24
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6802
Download HIP data (tab-delimited text)  (excel)
Gene:ERG11(YHR007C)|FD-Score:4.4|P-value:5.31E-6|Clearance:0.32||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.05||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:EXO70(YJL085W)|FD-Score:3.45|P-value:2.79E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.42||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:-3.11|P-value:9.23E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:4.08|P-value:2.25E-5|Clearance:0.53||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:8.23|P-value:9.66E-17|Clearance:3.7||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:MCM6(YGL201C)|FD-Score:-3.11|P-value:9.44E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:PUP3(YER094C)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:SLD5(YDR489W)|FD-Score:-3.88|P-value:5.21E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SUP45(YBR143C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.1||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TOM40(YMR203W)|FD-Score:4.53|P-value:2.97E-6|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:ERG11(YHR007C)|FD-Score:4.4|P-value:5.31E-6|Clearance:0.32||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.05||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:EXO70(YJL085W)|FD-Score:3.45|P-value:2.79E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.42||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:-3.11|P-value:9.23E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:4.08|P-value:2.25E-5|Clearance:0.53||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:8.23|P-value:9.66E-17|Clearance:3.7||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:MCM6(YGL201C)|FD-Score:-3.11|P-value:9.44E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:PUP3(YER094C)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:SLD5(YDR489W)|FD-Score:-3.88|P-value:5.21E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SUP45(YBR143C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.1||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TOM40(YMR203W)|FD-Score:4.53|P-value:2.97E-6|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6802
Download HOP data (tab-delimited text)  (excel)
Gene:ARG2(YJL071W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARL1(YBR164C)|FD-Score:-4.51|P-value:3.23E-6||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASG7(YJL170C)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP15(YPL271W)|FD-Score:-3.72|P-value:9.84E-5||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:COX20(YDR231C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:CUE5(YOR042W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUL3(YGR003W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CWH43(YCR017C)|FD-Score:5.83|P-value:2.77E-9||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:ECM2(YBR065C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:FKH1(YIL131C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FRE4(YNR060W)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUS2(YMR232W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:MED1(YPR070W)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MFM1(YPL060W)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MIG1(YGL035C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MNN10(YDR245W)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MOH1(YBL049W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSS18(YPR134W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:PAF1(YBR279W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PHO5(YBR093C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO87(YCR037C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:RDL2(YOR286W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RTT101(YJL047C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SDL1(YIL167W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:TUP1(YCR084C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:URE2(YNL229C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAC7(YNL054W)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:YDL041W(YDL041W_d)|FD-Score:-4.61|P-value:1.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YGR035C(YGR035C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YKL053W(YKL053W_d)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR241W(YLR241W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YML082W(YML082W_p)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR074C(YMR074C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YOR015W(YOR015W_d)|FD-Score:4|P-value:3.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR238W(YOR238W_p)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ZRC1(YMR243C)|FD-Score:7.41|P-value:6.13E-14||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ZRT2(YLR130C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:ARG2(YJL071W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARL1(YBR164C)|FD-Score:-4.51|P-value:3.23E-6||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASG7(YJL170C)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP15(YPL271W)|FD-Score:-3.72|P-value:9.84E-5||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:COX20(YDR231C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:CUE5(YOR042W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUL3(YGR003W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CWH43(YCR017C)|FD-Score:5.83|P-value:2.77E-9||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:ECM2(YBR065C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:FKH1(YIL131C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FRE4(YNR060W)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUS2(YMR232W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:MED1(YPR070W)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MFM1(YPL060W)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MIG1(YGL035C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MNN10(YDR245W)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MOH1(YBL049W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSS18(YPR134W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:PAF1(YBR279W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PHO5(YBR093C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO87(YCR037C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:RDL2(YOR286W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RTT101(YJL047C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SDL1(YIL167W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:TUP1(YCR084C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:URE2(YNL229C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAC7(YNL054W)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:YDL041W(YDL041W_d)|FD-Score:-4.61|P-value:1.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YGR035C(YGR035C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YKL053W(YKL053W_d)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR241W(YLR241W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YML082W(YML082W_p)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR074C(YMR074C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YOR015W(YOR015W_d)|FD-Score:4|P-value:3.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR238W(YOR238W_p)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ZRC1(YMR243C)|FD-Score:7.41|P-value:6.13E-14||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ZRT2(YLR130C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL205C8.239.66E-173.70HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YMR203W4.532.97E-60.12TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YHR007C4.405.31E-60.32ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDR047W4.082.25E-50.53HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YBR143C3.551.94E-40.10SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YJL085W3.452.79E-40.00EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL001C3.452.83E-40.05ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YMR277W3.403.34E-40.42FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YGR116W2.990.001410.01SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YJL031C2.980.001460.11BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YJR023C_d2.860.002100.07YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YDR041W2.790.002610.01RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YOR218C_d2.790.002670.08YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YGL074C_d2.710.003400.12YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YDL147W2.580.004890.02RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR243C7.416.13E-14ZRC1Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
YCR017C5.832.77E-9CWH43Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
YJL071W4.395.70E-6ARG2Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
YCR037C4.191.38E-5PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YBL049W4.171.55E-5MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YMR074C4.151.66E-5YMR074CProtein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
YOR042W4.111.96E-5CUE5Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YOR015W_d4.003.19E-5YOR015W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNR060W3.894.97E-5FRE4Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
YOR286W3.855.81E-5RDL2Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
YMR232W3.711.02E-4FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YJL047C3.591.63E-4RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YIL167W3.541.97E-4SDL1Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase
YBR093C3.512.22E-4PHO5Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
YLR241W_p3.472.61E-4YLR241W_pPutative protein of unknown function, may be involved in detoxification

GO enrichment analysis for SGTC_1540
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1531.88E-32SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0740741heme biosynthesis & mitochondrial translocase
0.1478.18E-30SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.0757576superoxide
0.1374.06E-26SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.0704225superoxide
0.1344.82E-25SGTC_208k072-0098 12.4 μMChemDiv (Drug-like library)59489010.0410959heme biosynthesis & mitochondrial translocase
0.1314.48E-24SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0394737heme biosynthesis & mitochondrial translocase
0.1301.72E-23SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0649351heme biosynthesis & mitochondrial translocase
0.1141.15E-18SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0597015heme biosynthesis & mitochondrial translocase
0.1141.30E-18SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.0735294heme biosynthesis & mitochondrial translocase
0.1134.13E-18SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0422535heme biosynthesis & mitochondrial translocase
0.1127.86E-18SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.0675676heme biosynthesis & mitochondrial translocase
0.1119.05E-18SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.0759494heme biosynthesis & mitochondrial translocase
0.1111.62E-17SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.0779221copper-dependent oxidative stress
0.1081.07E-16SGTC_6740498-0004 451.0 μMChemDiv (Drug-like library)42314730.0689655heme requiring
0.0992.57E-14SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0576923heme biosynthesis & mitochondrial translocase
0.0961.20E-13SGTC_2541muurolladie-3-one 83.1 μMMicrosource (Natural product library)39894190.0704225excess fatty acid

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1528arabinosylhypoxanthine74.6 μM0.535714804TimTec (Pure natural product library)268.22608-2.24946
SGTC_1539benzyladenosine56 μM0.36764792208TimTec (Pure natural product library)357.363860.25848
SGTC_15346-azauridine81.6 μM0.333333233502TimTec (Pure natural product library)245.18944-2.43247RNA pol III & RNase P/MRP
SGTC_2615-fluorouridine15.38 μM0.3278691821Miscellaneous262.191843-2.54147exosome
SGTC_1879gemcitabine100 μM0.26865760750Miscellaneous263.198146-1.39436
SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid61.9 μM0.26760666535TimTec (Pure natural product library)323.196522-2.46858
SGTC_1956st07708554 μM0.22368424208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.21794914077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes
SGTC_1569carminic acid40.6 μM0.2151914749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2490carminic acid95.39 μM0.2151914749TimTec (Pure natural product library)492.3864-0.436913
SGTC_248streptozotocin5.23 mM0.2121215300Miscellaneous265.2206-2.28558DNA damage response